Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,508,681 C→A 100% F198L (TTC→TTA)  ydcU → putative spermidine/putrescine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,508,6810CA99.3% 488.5 / ‑4.0 154F198L (TTC→TTA) ydcUputative spermidine/putrescine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (87/65);  total (88/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 154 total aligned reads displayed.

GGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATC  >  CP009273/1508538‑1508825
                                                                                                                                               |                                                                                                                                                
ggCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGa                                                                                                                                              <  1:2610412/148‑1 (MQ=255)
ggCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTGTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGa                                                                                                                                              >  2:701408/1‑148 (MQ=255)
    tGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGGCGTTAATTATCCt                                                                                                                                          >  1:2694970/1‑148 (MQ=255)
    tGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCt                                                                                                                                          >  2:1425727/1‑148 (MQ=255)
      gCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGc                                                                                                                                        >  2:3395284/1‑148 (MQ=255)
      gCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGc                                                                                                                                        >  2:1156866/1‑148 (MQ=255)
      gCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGc                                                                                                                                        >  1:2001879/1‑148 (MQ=255)
      gCTCAGGGGTTTTTACAACATCTTGGGCTGGAACCACAGCTGACTGCGTTCCTTACATTACCTCCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCGTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGc                                                                                                                                        >  2:1416692/1‑148 (MQ=255)
       cTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGcc                                                                                                                                       >  1:2755701/1‑148 (MQ=255)
       cTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTGTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGcc                                                                                                                                       >  2:1552611/1‑148 (MQ=255)
          aGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGt                                                                                                                                    <  2:416219/148‑1 (MQ=255)
           gTGGTTTTTACAACATCTTGGTCTGGAACCACTGCTGACTTCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGtt                                                                                                                                   <  1:520454/148‑1 (MQ=255)
           gTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGtt                                                                                                                                   >  1:1067196/1‑148 (MQ=255)
            tGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTc                                                                                                                                  <  2:772243/148‑1 (MQ=255)
            tGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTc                                                                                                                                  <  2:771361/148‑1 (MQ=255)
             ggTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCa                                                                                                                                 <  2:256187/148‑1 (MQ=255)
              gTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAg                                                                                                                                >  1:2232080/1‑148 (MQ=255)
              gTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAg                                                                                                                                >  2:1719779/1‑148 (MQ=255)
               tttttACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAgg                                                                                                                               >  1:48110/1‑148 (MQ=255)
                  ttACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAggcgg                                                                                                                            <  1:2594996/148‑1 (MQ=255)
                    acaacaTCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGgcggcg                                                                                                                          <  2:2479571/148‑1 (MQ=255)
                    acaacaTCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGgcggcg                                                                                                                          <  1:1156866/148‑1 (MQ=255)
                      aacaTCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCt                                                                                                                        >  2:511740/1‑148 (MQ=255)
                       acaTCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCtt                                                                                                                       >  1:3549497/1‑148 (MQ=255)
                        caTCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTg                                                                                                                      <  2:3079374/148‑1 (MQ=255)
                         aTCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGa                                                                                                                     >  2:2504857/1‑148 (MQ=255)
                         aTCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGa                                                                                                                     >  2:2481214/1‑148 (MQ=255)
                          tCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAg                                                                                                                    <  2:1670462/148‑1 (MQ=255)
                          tCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAg                                                                                                                    <  1:3022424/148‑1 (MQ=255)
                          tCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAg                                                                                                                    <  2:3084765/148‑1 (MQ=255)
                               ggCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTGTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGttt                                                                                                               >  1:1716686/1‑148 (MQ=255)
                               ggCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGttt                                                                                                               >  1:1716825/1‑148 (MQ=255)
                                 cTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGttttc                                                                                                             <  2:157960/148‑3 (MQ=255)
                                   ggAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTgccg                                                                                                           >  2:3509022/1‑148 (MQ=255)
                                       ccACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGt                                                                                                       <  2:1348347/148‑1 (MQ=255)
                                       ccACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGt                                                                                                       >  2:152972/1‑148 (MQ=255)
                                         aCTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCa                                                                                                     >  2:2590657/1‑148 (MQ=255)
                                          ctgctgACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCAt                                                                                                    >  2:3207139/1‑148 (MQ=255)
                                          ctgctgACTGCGTTCCTTACATTACCTGCGGTGGGAGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGGTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCAt                                                                                                    <  2:3546838/148‑1 (MQ=255)
                                               gACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCAttgttg                                                                                               >  2:544188/1‑148 (MQ=255)
                                               gACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCAttgttg                                                                                               >  2:2336969/1‑148 (MQ=255)
                                               gACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCAttgttg                                                                                               >  1:2688899/1‑148 (MQ=255)
                                                 cTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCa                                                                                             <  2:2268842/148‑1 (MQ=255)
                                                     gTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCg                                                                                         <  1:3336603/148‑1 (MQ=255)
                                                      ttCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGt                                                                                        >  2:258955/1‑148 (MQ=255)
                                                       tCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCTCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTc                                                                                       >  1:1675249/1‑148 (MQ=255)
                                                       tCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCTCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTc                                                                                       >  1:1675212/1‑148 (MQ=255)
                                                        ccTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCg                                                                                      <  1:1752020/148‑1 (MQ=255)
                                                            aCATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTg                                                                                  >  1:763418/1‑148 (MQ=255)
                                                            aCATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTg                                                                                  <  1:1762453/148‑1 (MQ=255)
                                                            aCATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTg                                                                                  >  2:2638417/1‑148 (MQ=255)
                                                              aTTACCTGCTGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCGGTCTGGTGGATCTGTCTAACAGGTTGACGATATTTATCCAGCACGTTCAGGCGGCGGTTGAGCGTTTGCGGCCGTCATTGGTGCAGGCGACGGCAGAt                                                                                >  1:2385305/1‑148 (MQ=255)
                                                              aTTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGAt                                                                                >  1:2674625/1‑148 (MQ=255)
                                                                tACCTGCGGTGGGCGGAAATACGCTGCCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGAtct                                                                              <  2:80244/148‑1 (MQ=255)
                                                                  ccTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCg                                                                            <  1:3549256/148‑1 (MQ=255)
                                                                  ccTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCg                                                                            <  2:1067196/148‑1 (MQ=255)
                                                                   cTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATTATACTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCATGCGTCGGCTGATCTCgg                                                                           >  1:2540655/1‑148 (MQ=255)
                                                                       ggTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCa                                                                       <  1:1347677/148‑1 (MQ=255)
                                                                           ggCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTc                                                                   >  2:3081652/1‑148 (MQ=255)
                                                                                   tACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATTATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACaa                                                           >  2:1393291/1‑148 (MQ=255)
                                                                                   tACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACaa                                                           <  2:3160928/148‑1 (MQ=255)
                                                                                   tACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACaa                                                           >  2:1934571/1‑148 (MQ=255)
                                                                                   tACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACaa                                                           <  2:1384654/148‑1 (MQ=255)
                                                                                     cGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAAc                                                         <  1:1288220/148‑1 (MQ=255)
                                                                                     cGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGGTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAAc                                                         >  1:644516/1‑148 (MQ=255)
                                                                                      gCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAAcc                                                        <  1:2451758/148‑1 (MQ=255)
                                                                                        tGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGACATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCtt                                                      >  2:1134365/1‑148 (MQ=255)
                                                                                              cTTCCGGGCTGGGGCGCTTTCTTGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTa                                                <  2:2912639/148‑1 (MQ=255)
                                                                                              cTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTa                                                <  2:2913640/148‑1 (MQ=255)
                                                                                                    ggCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATgtggt                                          >  2:482662/1‑148 (MQ=255)
                                                                                                    ggCTGGGGCGCTTTCTGGTGTTTCTCTATATATGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTTCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATgtggt                                          >  1:2200733/1‑148 (MQ=255)
                                                                                                     gCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATgtggtg                                         >  2:678841/1‑148 (MQ=255)
                                                                                                      cTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGtgc                                        <  1:1031158/148‑1 (MQ=255)
                                                                                                         gggCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTTAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGtgctgc                                     >  2:3484713/1‑148 (MQ=255)
                                                                                                         gggCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGtgctgc                                     <  2:507275/148‑1 (MQ=255)
                                                                                                         gggCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGtgctgc                                     >  2:2083570/1‑148 (MQ=255)
                                                                                                           gcgcTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCATTCGCTATGTGGTGCTGCCg                                   >  1:2337951/1‑148 (MQ=255)
                                                                                                           gcgcTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCg                                   <  1:3051423/148‑1 (MQ=255)
                                                                                                               tttCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTgg                               >  2:1052473/1‑148 (MQ=255)
                                                                                                                 tCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCa                             <  2:1675249/148‑1 (MQ=255)
                                                                                                                      tgtTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATccc                        >  2:612787/1‑148 (MQ=255)
                                                                                                                      tgtTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATccc                        >  2:612246/1‑148 (MQ=255)
                                                                                                                      tgtTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCCTTTGCCGCCGTAAATTTTTCAGGCGACGGCTGATCTCGGCGCACGTCCACGACAAACCTGTCGCTATCTGGTGCTGCCGCAGGCAAAccc                        >  1:344500/1‑148 (MQ=255)
                                                                                                                               atatCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGc               >  2:278691/1‑148 (MQ=255)
                                                                                                                                  tCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGc            >  1:1926764/1‑148 (MQ=255)
                                                                                                                                   cTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCCATCCCGGGTATTGCCGCt           >  1:330049/1‑148 (MQ=255)
                                                                                                                                   cTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCt           <  2:216493/148‑1 (MQ=255)
                                                                                                                                   cTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCt           >  2:1962889/1‑148 (MQ=255)
                                                                                                                                     ggTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTgg         >  1:1156997/1‑148 (MQ=255)
                                                                                                                                       ttGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGct       >  2:3401432/1‑148 (MQ=255)
                                                                                                                                       ttGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGct       <  1:2777189/148‑1 (MQ=255)
                                                                                                                                       ttGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGct       <  1:997246/148‑1 (MQ=255)
                                                                                                                                         gCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGctct     >  1:1782328/1‑148 (MQ=255)
                                                                                                                                          ccGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTa    <  2:3288679/148‑1 (MQ=255)
                                                                                                                                          ccGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTa    <  2:313247/148‑1 (MQ=255)
                                                                                                                                           cGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTAt   >  1:1879191/1‑148 (MQ=255)
                                                                                                                                           cGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTAt   >  1:1880663/1‑148 (MQ=255)
                                                                                                                                            gTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATc  <  1:3178912/148‑1 (MQ=255)
                                                                                                                                            gTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATc  <  2:3549497/148‑1 (MQ=255)
                                                                                                                                            gTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATc  <  2:2113414/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
GGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATC  >  CP009273/1508538‑1508825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑