Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,537,806 A→T 100% L171* (TTA→TAA)  narU ← nitrate/nitrite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,537,8060AT95.1% 569.9 / 15.1 182L171* (TTA→TAA) narUnitrate/nitrite transporter
Reads supporting (aligned to +/- strand):  ref base A (5/4);  new base T (97/76);  total (102/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 182 total aligned reads displayed.

CGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTT  >  CP009273/1537661‑1537952
                                                                                                                                                 |                                                                                                                                                  
cGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCACATACAGGTACAAAAATGACCAGCGGT‑CAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATc                                                                                                                                                 >  2:2651641/1‑148 (MQ=255)
cGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTAt                                                                                                                                                  >  2:2423868/1‑148 (MQ=255)
    aCCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGc                                                                                                                                              <  2:847820/148‑1 (MQ=255)
    aCCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGc                                                                                                                                              <  1:1958870/148‑1 (MQ=255)
    aCCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGc                                                                                                                                              <  1:1957087/148‑1 (MQ=255)
        aaaTCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCCGCGGTGCACCCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCAtt                                                                                                                                          >  2:119442/1‑148 (MQ=255)
          aTCGATGCGTCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACACAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTTAGTTTCCTTATCCGCCATTaa                                                                                                                                        <  2:3141278/148‑1 (MQ=255)
          aTCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTaa                                                                                                                                        >  1:2803518/1‑148 (MQ=255)
          aTCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTaa                                                                                                                                        <  2:3141319/148‑1 (MQ=255)
           tCCATGCGGCATTCGCCAGCGACATCACCGAACCTTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAAt                                                                                                                                       <  2:2822545/148‑1 (MQ=255)
           tCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAAt                                                                                                                                       >  1:1012572/1‑148 (MQ=255)
               tGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCa                                                                                                                                   <  2:3478224/148‑1 (MQ=255)
                   gCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAagag                                                                                                                               >  2:645278/1‑148 (MQ=255)
                    cATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAgc                                                                                                                              >  2:240471/1‑148 (MQ=255)
                     aTTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAgcg                                                                                                                             <  2:2050264/148‑1 (MQ=255)
                       tCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCt                                                                                                                           >  1:670991/1‑148 (MQ=255)
                       tCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCt                                                                                                                           >  1:2721693/1‑148 (MQ=255)
                          ccAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTccc                                                                                                                        <  2:1800747/148‑1 (MQ=255)
                          ccAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTccc                                                                                                                        <  2:1013170/148‑1 (MQ=255)
                           cAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCt                                                                                                                       <  2:43823/148‑1 (MQ=255)
                             gCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTg                                                                                                                     <  1:3471694/148‑1 (MQ=255)
                                aCATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGttt                                                                                                                  <  1:1860725/148‑1 (MQ=255)
                                 cATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTg                                                                                                                 >  2:1836312/1‑148 (MQ=255)
                                    cACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCt                                                                                                              <  1:1281258/148‑1 (MQ=255)
                                    cACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCt                                                                                                              <  2:2566730/148‑1 (MQ=255)
                                      ccGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCttt                                                                                                            >  2:2977681/1‑148 (MQ=255)
                                        gAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTg                                                                                                          >  1:1566506/1‑148 (MQ=255)
                                           ccGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGaa                                                                                                       <  1:3098851/148‑1 (MQ=255)
                                           ccGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGaa                                                                                                       <  1:3084121/148‑1 (MQ=255)
                                           ccGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGaa                                                                                                       <  1:2976325/148‑1 (MQ=255)
                                            cGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGaaa                                                                                                      >  2:645545/1‑148 (MQ=255)
                                                  ccTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAgaagaa                                                                                                <  1:3400833/148‑1 (MQ=255)
                                                   cTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGaaa                                                                                               <  1:2548035/148‑1 (MQ=255)
                                                   cTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGaaa                                                                                               <  1:160929/148‑1 (MQ=255)
                                                   cTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGaaa                                                                                               <  2:2217878/148‑1 (MQ=255)
                                                    tGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAAc                                                                                              <  1:2206194/148‑1 (MQ=255)
                                                     gCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACt                                                                                             >  1:3130672/1‑148 (MQ=255)
                                                      cGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTg                                                                                            <  1:1597485/148‑1 (MQ=255)
                                                      cGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTg                                                                                            <  1:120710/148‑1 (MQ=255)
                                                        gTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGat                                                                                          <  2:2254125/148‑1 (MQ=255)
                                                          aCGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGatat                                                                                        <  1:1710579/148‑1 (MQ=255)
                                                            gCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTg                                                                                      >  1:112705/1‑148 (MQ=255)
                                                            gCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTg                                                                                      >  2:1738307/1‑148 (MQ=255)
                                                                          gaAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGc                                                                        >  1:927484/1‑148 (MQ=255)
                                                                           aaaGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCa                                                                       >  2:1704269/1‑148 (MQ=255)
                                                                               gCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGt                                                                   <  2:3395024/148‑1 (MQ=255)
                                                                                    tACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCa                                                              >  1:3446083/1‑148 (MQ=255)
                                                                                      cAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAaaga                                                                                                    <  2:2932561/108‑1 (MQ=255)
                                                                                          tACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCa                                                        >  2:1119992/1‑148 (MQ=255)
                                                                                            cAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCaaa                                                      <  2:3544424/148‑1 (MQ=255)
                                                                                             aaaaaTGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCaaaa                                                     <  1:2611987/148‑1 (MQ=255)
                                                                                               aaaTGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAAcc                                                   >  1:991584/1‑148 (MQ=255)
                                                                                                aaTGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCg                                                  >  1:3497126/1‑148 (MQ=255)
                                                                                                   gACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCAt                                               <  1:761067/148‑1 (MQ=255)
                                                                                                   gACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCCGTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCAt                                               <  2:3134722/148‑1 (MQ=255)
                                                                                                     ccAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAg                                             >  2:1269438/1‑148 (MQ=255)
                                                                                                     ccAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAg                                             >  2:1992487/1‑148 (MQ=255)
                                                                                                      cAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGc                                            >  2:2695409/1‑148 (MQ=255)
                                                                                                      cAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGc                                            >  1:2736149/1‑148 (MQ=255)
                                                                                                      cAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGc                                            >  1:841114/1‑148 (MQ=255)
                                                                                                        gCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCaa                                          <  2:3100639/148‑1 (MQ=255)
                                                                                                         cGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCaaa                                         >  2:2660670/1‑148 (MQ=255)
                                                                                                         cGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCaaa                                         >  1:1143551/1‑148 (MQ=255)
                                                                                                         cGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCaaa                                         >  1:1143510/1‑148 (MQ=255)
                                                                                                          ggTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAg                                        <  2:407059/148‑1 (MQ=255)
                                                                                                          ggTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAg                                        <  2:1955349/148‑1 (MQ=255)
                                                                                                           gTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGc                                       >  2:2890632/1‑148 (MQ=255)
                                                                                                           gTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACATGCAAAACCGCATAGCAAAGc                                       >  2:2520029/1‑148 (MQ=255)
                                                                                                            tGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcg                                      >  1:2997901/1‑148 (MQ=255)
                                                                                                            gtcAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcg                                      <  1:2651641/146‑1 (MQ=255)
                                                                                                              cAACCAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcgat                                    <  1:2319241/148‑1 (MQ=255)
                                                                                                                 ccAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcgataac                                 >  1:2076391/1‑148 (MQ=255)
                                                                                                                 ccAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcgataac                                 >  2:1433524/1‑148 (MQ=255)
                                                                                                                  cAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcgataacg                                <  1:1509912/148‑1 (MQ=255)
                                                                                                                  cAGCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcgataacg                                >  2:3521345/1‑148 (MQ=255)
                                                                                                                    gCTGCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGcgataacgat                              <  1:3277106/148‑1 (MQ=255)
                                                                                                                       gCATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATaaa                           >  1:1009675/1‑148 (MQ=255)
                                                                                                                        cATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAat                          <  1:242367/148‑1 (MQ=255)
                                                                                                                        cATTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAat                          >  1:636284/1‑148 (MQ=255)
                                                                                                                         aTTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAata                         >  1:2545521/1‑148 (MQ=255)
                                                                                                                         aTTACACTTACACCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAata                         >  2:1664749/1‑148 (MQ=255)
                                                                                                                           tacacttacacCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATc                       >  2:509728/1‑148 (MQ=255)
                                                                                                                           tacacttacacCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATc                       >  1:63306/1‑148 (MQ=255)
                                                                                                                             cacttacacCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATccc                     >  2:907500/1‑148 (MQ=255)
                                                                                                                                 tacacCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCaaaa                 >  2:2608292/1‑148 (MQ=255)
                                                                                                                                 tacacCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCaaaa                 >  1:1465755/1‑148 (MQ=255)
                                                                                                                                   cacCTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAACACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAgg               >  2:2282100/1‑148 (MQ=255)
                                                                                                                                      cTAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGt            <  1:2085836/148‑1 (MQ=255)
                                                                                                                                       tAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTa           <  2:927484/148‑1 (MQ=255)
                                                                                                                                       tAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTa           <  2:2724694/148‑1 (MQ=255)
                                                                                                                                       tAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTa           <  2:1240406/148‑1 (MQ=255)
                                                                                                                                       tAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTa           <  1:1822057/148‑1 (MQ=255)
                                                                                                                                       tAAGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTa           <  1:1987147/148‑1 (MQ=255)
                                                                                                                                        aaGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTAt          >  1:2006849/1‑148 (MQ=255)
                                                                                                                                        aaGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTAt          >  2:839956/1‑148 (MQ=255)
                                                                                                                                        aaGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTAt          <  2:1009675/148‑1 (MQ=255)
                                                                                                                                        aaGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTAt          >  1:48217/1‑148 (MQ=255)
                                                                                                                                         aGTTTCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTAtt         >  1:3262022/1‑148 (MQ=255)
                                                                                                                                           tttCCTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCg       >  1:1273429/1‑148 (MQ=255)
                                                                                                                                              ccTTATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGAt    >  2:3279903/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CGGTACCCAAATCCATGCGGCATTCGCCAGCGACATCACCGAACCGTCGGCCTGCGGTACGCCATTGACGCCGAGAAAGGCAAATACAGGTACAAAAATGACCAGCGGTGCAACCAGCTGCATTACACTTACACCTAAGTTTCCTAATCCGCCATTAATCCCAAGAGCGCTCCCTTGTTTGGCTTTTGGAAAGAAGAAACTGATATTGCCCATGCTCGAAGCAAAGTTTGCACCTGCAAAACCGCATAGCAAAGCGATAACGATAAATATCCCAAAAGGAGTATTCGGATTT  >  CP009273/1537661‑1537952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑