Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,565,497	G→A	100%	S269L (TCG→TTG)	gadB ←	glutamate decarboxylase B, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,565,497	0	G	A	100.0%	116.6 / NA	52	S269L (TCG→TTG)	gadB	glutamate decarboxylase B, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/21);  total (31/21)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGCGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTT  >  CP009273/1565458‑1565641                                        |                                                                                                                                                 aGCGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTAt                                      <  2:3361135/148‑1 (MQ=14)  gCGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATc                                     <  1:743980/148‑1 (MQ=17)  gCGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATc                                     <  1:3167165/148‑1 (MQ=17)   cGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCg                                    >  1:689550/1‑148 (MQ=17)   cGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCg                                    >  1:1364520/1‑148 (MQ=17)   cGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCg                                    >  1:1746054/1‑148 (MQ=17)   cGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCg                                    >  1:2590066/1‑148 (MQ=17)   cGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCg                                    >  1:3062329/1‑148 (MQ=17)   cGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCg                                    >  1:3062199/1‑148 (MQ=17)   cGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCg                                    >  1:3062293/1‑148 (MQ=17)     gAGCCAGACCGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGc                                  <  2:1131574/148‑1 (MQ=17)     gAGCCAGACCGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGc                                  <  1:3022886/148‑1 (MQ=17)        ccAGACCGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTg                               >  2:2206455/1‑148 (MQ=17)          aGACCGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGa                             >  2:759355/1‑148 (MQ=17)          aGACCGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGa                             >  2:1725695/1‑148 (MQ=17)          aGACCGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGa                             >  2:1725925/1‑148 (MQ=17)          aGACCGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGa                             <  1:3430587/148‑1 (MQ=17)           taCCGAATTTATGGCCTGAAGCACTGATCAATTTCACACGAGGCAGGCGGAAGTCCCAGACGAAATCCGGGGCGACGAACGGTGCCAGCAAGACACCGCTGGCAGCGTCGATGTGCATGTCCATGTCGATACCGGTATCGGCCTGGaa                            >  2:2663483/2‑148 (MQ=11)           gACCGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGaa                            >  1:3181470/1‑148 (MQ=17)           gACCGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGaa                            <  2:814848/148‑1 (MQ=17)            aCCGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAAt                           >  2:721384/1‑148 (MQ=17)            aCCGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAAt                           >  1:2368986/1‑148 (MQ=17)             ccGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAAtt                          <  1:842623/148‑1 (MQ=17)              cGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAAttt                         <  1:3533970/148‑1 (MQ=17)              cGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAAttt                         >  2:72961/1‑148 (MQ=17)              cGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAAttt                         >  2:76115/1‑148 (MQ=17)              cGAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAAttt                         >  2:76476/1‑148 (MQ=17)               gAATTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGGCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTAGATACCGGTATAGGCCTGGAATTTa                        <  1:3340867/148‑1 (MQ=255)                aaTTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTAt                       <  1:1360505/148‑1 (MQ=17)                aaTTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTAt                       <  2:3153342/148‑1 (MQ=17)                aaTTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTAt                       <  2:3153299/148‑1 (MQ=17)                aaTGTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCGAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGTTACCGGTATCGGCCTGGAATTTAt                       <  2:3153274/148‑1 (MQ=255)                 aTTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATc                      >  2:2490391/1‑148 (MQ=17)                 aTTTATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATc                      >  2:2489484/1‑148 (MQ=17)                  tttATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATcc                     <  1:1195416/148‑1 (MQ=17)                  tttATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATcc                     >  1:2871687/1‑148 (MQ=17)                  tttATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATcc                     <  2:745985/148‑1 (MQ=17)                  tttATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATcc                     >  2:649569/1‑148 (MQ=17)                  tttATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATcc                     >  2:649557/1‑148 (MQ=17)                  tgtATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATcc                     <  2:745181/146‑1 (MQ=17)                   ttATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCa                    >  1:2911730/1‑148 (MQ=17)                   ttATGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCa                    >  1:169656/1‑148 (MQ=17)                      tGGCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAgcg                 >  2:1279133/1‑148 (MQ=17)                        gCCTGAAGCACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCa               >  2:1335445/1‑148 (MQ=17)                                cACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAg       >  2:1528151/1‑148 (MQ=17)                                cACTGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAg       <  2:2048276/148‑1 (MQ=17)                                   tGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCgg    <  1:3417660/148‑1 (MQ=17)                                   tGATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCgg    <  1:203648/148‑1 (MQ=17)                                    gATCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGt   <  2:637222/148‑1 (MQ=17)                                     aTCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCTGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGtt  >  2:694081/1‑148 (MQ=17)                                     aTCAATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGtt  >  2:446159/1‑148 (MQ=17)                                     aTCAATTTCACACGCGACAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGtt  >  2:2520859/1‑148 (MQ=17)                                        |                                                                                                                                                 AGCGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTT  >  CP009273/1565458‑1565641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑