Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,723,809 G→T 100% E156* (GAA→TAA)  lhr → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,723,8090GT95.9% 579.0 / 14.5 198E156* (GAA→TAA) lhrputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (8/0);  new base T (88/102);  total (96/102)
Fisher's exact test for biased strand distribution p-value = 2.61e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 198 total aligned reads displayed.

CCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGC  >  CP009273/1723664‑1723953
                                                                                                                                                 |                                                                                                                                                
cccGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGaa                                                                                                                                                >  1:1590576/1‑148 (MQ=255)
cccGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGaa                                                                                                                                                >  1:1590850/1‑148 (MQ=255)
 ccGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAAt                                                                                                                                               <  2:1778956/148‑1 (MQ=255)
 ccGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAAt                                                                                                                                               >  1:3051348/1‑148 (MQ=255)
     tGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCAct                                                                                                                                           >  2:2539178/1‑148 (MQ=255)
     tGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCAct                                                                                                                                           >  1:3342954/1‑148 (MQ=255)
     tGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCAct                                                                                                                                           <  2:2094680/148‑1 (MQ=255)
       aaGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATCTTCTGATCACCACACCCTAATCActct                                                                                                                                         >  1:2813509/1‑148 (MQ=255)
          ggTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATc                                                                                                                                      <  2:3460276/148‑1 (MQ=255)
            tATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTg                                                                                                                                    <  2:2791759/148‑1 (MQ=255)
            tATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTg                                                                                                                                    <  2:3278730/148‑1 (MQ=255)
             aTTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGa                                                                                                                                   <  1:3171008/148‑1 (MQ=255)
               tGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATg                                                                                                                                 <  2:3027515/148‑1 (MQ=255)
               tGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATg                                                                                                                                 >  2:257845/1‑148 (MQ=255)
                  cGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTg                                                                                                                              <  1:3286116/148‑1 (MQ=255)
                     tGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGAcc                                                                                                                           >  1:2866594/1‑148 (MQ=255)
                     tGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGAcc                                                                                                                           <  2:1269938/148‑1 (MQ=255)
                     tGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGAcc                                                                                                                           >  1:1088015/1‑148 (MQ=255)
                        aCGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTcc                                                                                                                        <  1:1942833/148‑1 (MQ=255)
                         cggcggcggCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTccc                                                                                                                       >  2:1702494/1‑148 (MQ=255)
                         cggcggcggCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTccc                                                                                                                       >  2:925948/1‑148 (MQ=255)
                                 gcgcggcgAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCcgcgcgcgc                                                                                                               <  1:16932/148‑1 (MQ=255)
                                  cgcggcgAAACGGAAGTCAATCTTCGCGTAGGTATCCGTACTGGCGATACGACTGCACAGGAACGCAGCAAACTCTCCCGTAATCCGCCGGATATTCTTATAACCACACCCTATTCACTCTATCTGATGCTGACCTCCcgcgcgcgcg                                                                                                              <  2:371909/148‑1 (MQ=255)
                                  cgcggcgAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCcgcgcgcgcg                                                                                                              <  2:2166693/148‑1 (MQ=255)
                                    cggcgAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGaa                                                                                                            <  1:2309347/148‑1 (MQ=255)
                                       cgAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACg                                                                                                         <  1:2439675/148‑1 (MQ=255)
                                       cgAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACg                                                                                                         <  1:1729928/148‑1 (MQ=255)
                                         aaaCGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCt                                                                                                       <  1:2528501/148‑1 (MQ=255)
                                         aaaCGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCt                                                                                                       <  2:270900/148‑1 (MQ=255)
                                         aaaCGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCt                                                                                                       >  2:3011880/1‑148 (MQ=255)
                                           aCGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTAc                                                                                                     >  1:2038733/1‑148 (MQ=255)
                                             ggAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTAcgc                                                                                                   <  2:1733906/148‑1 (MQ=255)
                                               aaGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACgcgg                                                                                                 >  1:659052/1‑148 (MQ=255)
                                               aaGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACgcgg                                                                                                 >  2:1293013/1‑148 (MQ=255)
                                               aaGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACgcgg                                                                                                 >  2:2901274/1‑148 (MQ=255)
                                                  tCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGt                                                                                              <  2:1712075/148‑1 (MQ=255)
                                                      tCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGaa                                                                                          <  2:536590/148‑1 (MQ=255)
                                                      tCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGaa                                                                                          <  1:612988/148‑1 (MQ=255)
                                                      tCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGaa                                                                                          <  1:222259/148‑1 (MQ=255)
                                                       cTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGaaa                                                                                         >  2:1241368/1‑148 (MQ=255)
                                                         tCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACg                                                                                       <  2:3408406/148‑1 (MQ=255)
                                                           gcgTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGt                                                                                     <  2:2050702/148‑1 (MQ=255)
                                                            cgTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTa                                                                                    >  2:949535/1‑148 (MQ=255)
                                                              tAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAat                                                                                  >  2:2033409/1‑148 (MQ=255)
                                                                 ggATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAattat                                                                               <  1:1732530/148‑1 (MQ=255)
                                                                   aTCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtg                                                                             <  1:2598735/148‑1 (MQ=255)
                                                                   aTCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtg                                                                             >  2:1231128/1‑148 (MQ=255)
                                                                   aTCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtg                                                                             <  2:1417795/148‑1 (MQ=255)
                                                                    tCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtga                                                                            >  2:1623829/1‑148 (MQ=255)
                                                                       gTACTGTCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTAAGCGGCGTCTAAAAGGTAATTATtgatga                                                                         <  2:2919641/148‑1 (MQ=255)
                                                                       gTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTCTCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtgatga                                                                         <  1:71438/148‑1 (MQ=255)
                                                                       gTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtgatga                                                                         <  1:2718593/148‑1 (MQ=255)
                                                                        tACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGaa                                                                        <  1:1314949/148‑1 (MQ=255)
                                                                         aCTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAg                                                                       <  2:2825505/148‑1 (MQ=255)
                                                                            ggCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTcc                                                                    <  1:844485/148‑1 (MQ=255)
                                                                                aTACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCAcgc                                                                >  1:1505213/1‑148 (MQ=255)
                                                                                aTACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCAcgc                                                                >  2:1917361/1‑148 (MQ=255)
                                                                                  aCGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCgg                                                              >  2:1253153/1‑148 (MQ=255)
                                                                                  aCGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCgg                                                              >  2:1253253/1‑148 (MQ=255)
                                                                                  aCGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCgg                                                              <  1:3132858/148‑1 (MQ=255)
                                                                                   cGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGt                                                             <  1:3502379/148‑1 (MQ=255)
                                                                                    gCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTa                                                            <  1:257845/148‑1 (MQ=255)
                                                                                       tGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCg                                                         <  2:796547/148‑1 (MQ=255)
                                                                                        gCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTACTCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCgg                                                        <  2:151740/148‑1 (MQ=255)
                                                                                        gCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCgg                                                        >  1:571990/1‑148 (MQ=255)
                                                                                         cacaGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCggg                                                       >  1:2159203/1‑148 (MQ=255)
                                                                                          acaGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGc                                                      <  2:1088015/148‑1 (MQ=255)
                                                                                             ggAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGt                                                   >  1:3216753/1‑148 (MQ=255)
                                                                                               aaCGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTaa                                                 >  2:3488338/1‑148 (MQ=255)
                                                                                               aaCGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTaa                                                 >  2:1724601/1‑148 (MQ=255)
                                                                                                aCGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTaaa                                                <  2:2490093/148‑1 (MQ=255)
                                                                                                 cGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAAc                                               >  2:573793/1‑148 (MQ=255)
                                                                                                   cagcaAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACgt                                             <  2:1022540/148‑1 (MQ=255)
                                                                                                    agcaAACTCACCCGTAATCCGCCGGATATACTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACgtg                                            <  2:1396126/148‑1 (MQ=255)
                                                                                                     gcaAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACgtgg                                           >  1:461930/1‑148 (MQ=255)
                                                                                                      caAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACgtggt                                          >  2:2531326/1‑148 (MQ=255)
                                                                                                      caAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACgtggt                                          >  1:2755382/1‑148 (MQ=255)
                                                                                                            cACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCAt                                    <  1:3005787/148‑1 (MQ=255)
                                                                                                             aCCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATc                                   >  2:3123895/1‑148 (MQ=255)
                                                                                                              cccGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCt                                  <  1:1241368/148‑1 (MQ=255)
                                                                                                              cccGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCt                                  >  1:1466817/1‑148 (MQ=255)
                                                                                                               ccGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTg                                 >  1:1522487/1‑148 (MQ=255)
                                                                                                                    aTCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGtt                            >  1:971130/1‑148 (MQ=255)
                                                                                                                         ccgGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTc                       >  2:1667724/1‑148 (MQ=255)
                                                                                                                         ccgGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTc                       >  1:257670/1‑148 (MQ=255)
                                                                                                                         ccgGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCGACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTc                       <  2:1492603/148‑1 (MQ=255)
                                                                                                                           ggATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTg                     >  1:1705073/1‑148 (MQ=255)
                                                                                                                           ggATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTg                     <  2:3212876/148‑1 (MQ=255)
                                                                                                                           ggATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTg                     >  1:1854231/1‑148 (MQ=255)
                                                                                                                            gATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTgg                    <  2:2309179/148‑1 (MQ=255)
                                                                                                                            gATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTgg                    >  2:2426725/1‑148 (MQ=255)
                                                                                                                             atatTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTCAACGTGGTGCGCATCTGGCGTTAAGTCTGGa                   >  2:3043714/1‑148 (MQ=255)
                                                                                                                                 tCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCgg               >  1:2895574/1‑148 (MQ=255)
                                                                                                                                 tCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCgg               <  1:2033409/148‑1 (MQ=255)
                                                                                                                                 tCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCgg               >  2:2634527/1‑148 (MQ=255)
                                                                                                                                 tCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCgg               >  1:519117/1‑148 (MQ=255)
                                                                                                                                  cTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGc              <  2:109732/148‑1 (MQ=255)
                                                                                                                                      tCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTgg                    <  2:2878872/138‑1 (MQ=255)
                                                                                                                                           acacCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATgcgc     >  1:3179134/1‑148 (MQ=255)
                                                                                                                                              cccTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCgctgc  >  2:695467/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGC  >  CP009273/1723664‑1723953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑