Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,735,082 G→T 42.9% V305V (GTG→GTT)  ydhC → putative arabinose efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,735,0820GT42.9% 191.7 / 220.4 198V305V (GTG→GTT) ydhCputative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (63/50);  new base T (36/49);  total (99/99)
Fisher's exact test for biased strand distribution p-value = 8.46e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 210 total aligned reads displayed.

TGGCTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTCCTGGCTG  >  CP009273/1734935‑1735229
                                                                                                                                                   |                                                                                                                                                   
tGGCTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCtgtgtg                                                                                                                                                     <  1:1165224/148‑1 (MQ=255)
 ggCTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGa                                                                                                                                                    >  2:591902/1‑148 (MQ=255)
 ggCTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGa                                                                                                                                                    <  2:137845/148‑1 (MQ=255)
  gCTGTCGCGCCGCGCTGCAGAAATGGCTAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTAt                                                                                                                                                   <  1:164765/148‑1 (MQ=255)
    tGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATgg                                                                                                                                                 >  1:3074347/1‑148 (MQ=255)
      tCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCg                                                                                                                                               >  2:1417375/1‑148 (MQ=255)
              cgcTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCaa                                                                                                                                       <  2:2050757/148‑1 (MQ=255)
               gcTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAAt                                                                                                                                      <  1:800926/148‑1 (MQ=255)
                      aaaTGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGa                                                                                                                               >  2:449435/1‑148 (MQ=255)
                       aaTGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGAt                                                                                                                              <  1:354333/148‑1 (MQ=255)
                       aaTGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGAt                                                                                                                              <  1:3456708/148‑1 (MQ=255)
                        aTGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATc                                                                                                                             <  1:1726322/148‑1 (MQ=255)
                        aTGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATc                                                                                                                             <  2:2087975/148‑1 (MQ=255)
                         tGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCt                                                                                                                            >  2:572376/1‑148 (MQ=255)
                         tGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCt                                                                                                                            >  2:572215/1‑148 (MQ=255)
                           gcaaggcaagCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTAc                                                                                                                          >  2:2281542/1‑148 (MQ=255)
                            caaggcaagCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTAcc                                                                                                                         <  2:2512672/148‑1 (MQ=255)
                            caaggcaagCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTAcc                                                                                                                         <  2:776467/148‑1 (MQ=255)
                             aaggcaagCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTAccc                                                                                                                        <  2:499969/148‑1 (MQ=255)
                              aggcaagCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCt                                                                                                                       <  2:2346595/148‑1 (MQ=255)
                              aggcaagCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCt                                                                                                                       <  1:3179641/148‑1 (MQ=255)
                               ggcaagCAGTTATTACCGTGGTTTCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTTATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTa                                                                                                                      <  2:966196/148‑1 (MQ=255)
                               ggcaagCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTa                                                                                                                      <  1:2265701/148‑1 (MQ=255)
                               ggcaagCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTa                                                                                                                      <  1:343817/148‑1 (MQ=255)
                               ggcaagCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTa                                                                                                                      >  1:2069713/1‑148 (MQ=255)
                               ggcaagCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTa                                                                                                                      <  2:1509512/148‑1 (MQ=255)
                                gcaagCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTAt                                                                                                                     <  1:1780595/148‑1 (MQ=255)
                                 caagCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTAtt                                                                                                                    >  1:2184775/1‑148 (MQ=255)
                                 caagCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTAtt                                                                                                                    >  2:2848566/1‑148 (MQ=255)
                                  aagCAGTTATTACCGTGGGTGCTGGTGCTGTTTGCTGTCAGCGTCAGTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTAttg                                                                                                                   <  2:2026099/148‑1 (MQ=255)
                                     cAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTAttgttg                                                                                                                >  1:1110809/1‑148 (MQ=255)
                                         tattaCCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGc                                                                                                            >  2:1079742/1‑148 (MQ=255)
                                           ttaCCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCTATTGCCAATGGCGCGATCTACCCTATTGTTGTCGccc                                                                                                          <  2:3229863/148‑1 (MQ=255)
                                           ttaCCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGccc                                                                                                          >  2:2211892/1‑148 (MQ=255)
                                               cgaggTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGgc                                                                                                      >  2:1746649/4‑148 (MQ=255)
                                                gTGGGTGCCCGTGCAGATAGCTGTCAGCGTCATTGCGACCGGGGCTGCGGGCTTCAAGAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGgcg                                                                                                     <  1:2109672/148‑1 (MQ=255)
                                                 tGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGgcgc                                                                                                    >  2:196594/1‑148 (MQ=255)
                                                 tGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGgcgc                                                                                                    >  1:3553357/1‑148 (MQ=255)
                                                      gCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGt                                                                                               <  2:3429759/148‑1 (MQ=255)
                                                        tGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTcc                                                                                             <  1:471380/148‑1 (MQ=255)
                                                             cTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTcc                                                                                        <  1:2327813/148‑1 (MQ=255)
                                                              tGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTccc                                                                                       <  1:1599946/148‑1 (MQ=255)
                                                                tttGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCcac                                                                                     >  1:1164225/1‑148 (MQ=255)
                                                                 ttGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCcaca                                                                                    <  1:1298263/148‑1 (MQ=255)
                                                                    cTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGGCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGc                                                                                 >  1:596912/1‑148 (MQ=255)
                                                                     tGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCa                                                                                >  2:2855211/1‑148 (MQ=255)
                                                                      gTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCaa                                                                               <  2:2755103/148‑1 (MQ=255)
                                                                        cAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACt                                                                             >  2:1829572/1‑148 (MQ=255)
                                                                        cAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACt                                                                             >  1:2005043/1‑148 (MQ=255)
                                                                         aGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTg                                                                            >  1:2818026/1‑148 (MQ=255)
                                                                           cGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGt                                                                          <  1:1619098/148‑1 (MQ=255)
                                                                             tCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTcg                                                                        <  1:982341/148‑1 (MQ=255)
                                                                             tCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTcg                                                                        >  1:578512/1‑148 (MQ=255)
                                                                             tCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTcg                                                                        >  1:2368372/1‑148 (MQ=255)
                                                                             tCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTcg                                                                        >  2:116094/1‑148 (MQ=255)
                                                                              cATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTcgc                                                                       >  2:2363873/1‑148 (MQ=255)
                                                                               aTTGCGACCTGGGCTGCGGGATTCATTAGCCATGTGTCGCTGGTCGAAATCCTGCTCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTTCCCTATTGTTGTCGCCCAGGCGCTGCGTCCCGTCCCACACGCAACTGGTcgcg                                                                      <  2:1001711/148‑1 (MQ=255)
                                                                                     aCCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAg                                                                <  1:3510985/148‑1 (MQ=255)
                                                                                      ccTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGc                                                               >  2:2550088/1‑148 (MQ=255)
                                                                                        tGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCATCCCACACGCAACTGGTCGCGCCGCAGCGt                                                             >  2:3085567/1‑148 (MQ=255)
                                                                                               cGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGaa                                                      <  1:2062662/148‑1 (MQ=255)
                                                                                                 ggCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAaca                                                    >  2:2239533/1‑148 (MQ=255)
                                                                                                  gCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAacac                                                   >  1:1854049/1‑148 (MQ=255)
                                                                                                     tCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACActct                                                >  2:3477504/1‑148 (MQ=255)
                                                                                                     tCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACActct                                                >  2:1879478/1‑148 (MQ=255)
                                                                                                       aTTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTc                                              >  2:2617074/1‑148 (MQ=255)
                                                                                                        ttAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGa                                                       >  1:1925851/1‑138 (MQ=255)
                                                                                                        ttAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGa                                                       <  2:124162/138‑1 (MQ=255)
                                                                                                         tAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCaa                                            <  1:2848566/148‑1 (MQ=255)
                                                                                                         tAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCaa                                            >  1:689924/1‑148 (MQ=255)
                                                                                                           gCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACt                                          >  2:1353685/1‑148 (MQ=255)
                                                                                                             cATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTgg                                        <  2:1471209/148‑1 (MQ=255)
                                                                                                             cATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTgg                                        <  2:1111956/148‑1 (MQ=255)
                                                                                                                 tgtCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCt                                    <  1:2629801/148‑1 (MQ=255)
                                                                                                                   tCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCtgt                                  >  1:2473823/1‑148 (MQ=255)
                                                                                                                      cTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCt                               <  1:3219737/148‑1 (MQ=255)
                                                                                                                      cTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCt                               <  2:3147037/148‑1 (MQ=255)
                                                                                                                       tGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCtt                              <  1:1350478/148‑1 (MQ=255)
                                                                                                                       tGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCtt                              <  1:1351841/148‑1 (MQ=255)
                                                                                                                        ggTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTc                             >  1:2323360/1‑148 (MQ=255)
                                                                                                                          tCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCt                           >  1:1239731/1‑148 (MQ=255)
                                                                                                                             aaaTCCTGATCCCATTCTGTGTTAGGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGc                        <  2:3564204/148‑1 (MQ=255)
                                                                                                                              aaTCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCa                       <  2:730941/148‑1 (MQ=255)
                                                                                                                                tCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAg                     >  1:2713632/1‑148 (MQ=255)
                                                                                                                                 ccTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGt                    >  2:376549/1‑148 (MQ=255)
                                                                                                                                  cTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTc                   <  2:144542/148‑1 (MQ=255)
                                                                                                                                    gATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTg                 <  2:3154602/148‑1 (MQ=255)
                                                                                                                                    gATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTg                 <  2:3009929/148‑1 (MQ=255)
                                                                                                                                     aTCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTgg                <  1:3450055/148‑1 (MQ=255)
                                                                                                                                        ccATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAg             <  1:3157304/148‑1 (MQ=255)
                                                                                                                                         cagtCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGt            <  2:3224375/145‑1 (MQ=255)
                                                                                                                                         cATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGt            >  1:1136386/1‑148 (MQ=255)
                                                                                                                                         cATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGt            >  1:2086394/1‑148 (MQ=255)
                                                                                                                                          aTTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGtt           >  1:2399575/1‑148 (MQ=255)
                                                                                                                                              tgtgttATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTCCt       >  2:2066819/1‑148 (MQ=255)
                                                                                                                                              tgtgtgATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTCCt       <  1:1079741/148‑1 (MQ=255)
                                                                                                                                                tgttATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTCCTgg     <  1:295643/148‑1 (MQ=255)
                                                                                                                                                tgttATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTCCTgg     >  1:1474258/1‑148 (MQ=255)
                                                                                                                                                  tgATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTCCTGGCt   >  1:489109/1‑148 (MQ=255)
                                                                                                                                                   gATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTCCTGGCTg  <  2:2196534/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGGCTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTCCTGGCTG  >  CP009273/1734935‑1735229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑