Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,769,763 T→A 100% intergenic (+62/‑81) aroD → / → ydiF 3‑dehydroquinate dehydratase/putative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,769,7630TA97.0% 739.1 / 8.5 229intergenic (+62/‑81)aroD/ydiF3‑dehydroquinate dehydratase/putative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit
Reads supporting (aligned to +/- strand):  ref base T (5/2);  new base A (133/89);  total (138/91)
Fisher's exact test for biased strand distribution p-value = 7.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 229 total aligned reads displayed.

TAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGG  >  CP009273/1769625‑1769907
                                                                                                                                          |                                                                                                                                                
  aaaaaaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAatat                                                                                                                                       <  1:3263678/148‑1 (MQ=255)
  aaaaaaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAatat                                                                                                                                       <  1:729666/148‑1 (MQ=255)
  aaaaaaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAatat                                                                                                                                       <  2:2110308/148‑1 (MQ=255)
  aaaaaaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAatat                                                                                                                                       <  2:157341/148‑1 (MQ=255)
    aaaaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATAttt                                                                                                                                     <  2:656449/148‑1 (MQ=255)
      aaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCg                                                                                                                                   >  2:2295919/1‑148 (MQ=255)
      aaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCg                                                                                                                                   >  1:2149789/1‑148 (MQ=255)
      aaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCg                                                                                                                                   >  2:1490286/1‑148 (MQ=255)
       cgCGTCTGCGCCAGGGAAAATCTCGTTAAATGATTTGCGCACGTTATTAACTATTTTACACCAGGAATAAGAAATAATATTTCGGGGGGAACACCCTCCCCGCCGAACT‑AAAAATATATTCAATCGTATTAAATAAAAATATTTCGTg                                                                                                                                 <  1:1678881/147‑1 (MQ=255)
       aGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGt                                                                                                                                  <  1:2177105/148‑1 (MQ=255)
         cGTCTGCGCCAGGGCAAATCTCGGTACATGATGTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGa                                                                                                                                <  2:3024032/148‑1 (MQ=255)
             tGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGtct                                                                                                                            >  1:99146/1‑148 (MQ=255)
              gcgcCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGtctc                                                                                                                           <  1:2334381/148‑1 (MQ=255)
              gcgcCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGtctc                                                                                                                           <  2:3470212/148‑1 (MQ=255)
                 ccAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCtgt                                                                                                                        <  2:1826802/148‑1 (MQ=255)
                    gggCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTgcg                                                                                                                     >  2:2855402/1‑148 (MQ=255)
                     ggCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGTAACACCCTCCCCGCCGAACTAAAAAATATATTCAAGCGTATTAAATAAAAATATTTCGTGAGTCTCTGTgcgc                                                                                                                    >  1:1189750/1‑148 (MQ=255)
                     ggCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTgcgc                                                                                                                    >  1:1475242/1‑148 (MQ=255)
                        aaaTCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTaa                                                                                                                 <  2:641123/148‑1 (MQ=255)
                          aTCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAAtt                                                                                                               >  2:689705/1‑148 (MQ=255)
                          aTCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAAtt                                                                                                               >  1:74568/1‑148 (MQ=255)
                           tctcGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATtc                                                                                                              >  2:1252510/1‑148 (MQ=255)
                           tctcGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATtc                                                                                                              >  1:653414/1‑148 (MQ=255)
                              cGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTcc                                                                                                           <  1:3493698/148‑1 (MQ=255)
                              cGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTcc                                                                                                           >  1:2912206/1‑148 (MQ=255)
                                gTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCAt                                                                                                         >  2:674318/1‑148 (MQ=255)
                                  aaaTGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCAttt                                                                                                       <  2:373720/148‑1 (MQ=255)
                                    aTGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTgg                                                                                                     <  1:455109/148‑1 (MQ=255)
                                    aTGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTgg                                                                                                     <  2:3521688/148‑1 (MQ=255)
                                    aTGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTgg                                                                                                     <  1:3146059/148‑1 (MQ=255)
                                      gATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCg                                                                                                   >  2:620773/1‑148 (MQ=255)
                                       aTTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGt                                                                                                  >  2:1746472/1‑148 (MQ=255)
                                        tttGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATAGTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTa                                                                                                 <  2:697200/148‑1 (MQ=255)
                                         ttGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAg                                                                                                <  2:1730748/148‑1 (MQ=255)
                                         ttGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAg                                                                                                >  1:3509002/1‑148 (MQ=255)
                                         gtGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAg                                                                                                <  2:1043926/147‑1 (MQ=255)
                                              cACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGaaa                                                                                           >  1:719943/1‑148 (MQ=255)
                                              cACGGTATTAACTATTGTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTACGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGaaa                                                                                           <  2:3252394/148‑1 (MQ=255)
                                                cGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAAt                                                                                         <  1:2573399/148‑1 (MQ=255)
                                                   tATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATcac                                                                                      <  2:2149795/148‑1 (MQ=255)
                                                   tATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATcac                                                                                      >  2:2249351/1‑148 (MQ=255)
                                                   tATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATcac                                                                                      >  2:1914046/1‑148 (MQ=255)
                                                      tAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATc                                                                                   >  1:2888175/1‑148 (MQ=255)
                                                      tAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCAGTTGGCGTAGGGAAAATCACATc                                                                                   >  1:19283/1‑148 (MQ=255)
                                                         cTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGa                                                                                >  2:896111/1‑148 (MQ=255)
                                                         cTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGa                                                                                >  2:2940568/1‑148 (MQ=255)
                                                         cTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGa                                                                                >  1:1644206/1‑148 (MQ=255)
                                                         cTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGa                                                                                <  1:964371/148‑1 (MQ=255)
                                                           aTTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAAt                                                                              >  2:494788/1‑148 (MQ=255)
                                                           aTTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAAt                                                                              <  1:186610/148‑1 (MQ=255)
                                                            ttttACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATc                                                                             >  1:3365120/1‑148 (MQ=255)
                                                                acacCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGa                                                                         >  1:1753323/1‑148 (MQ=255)
                                                                  acCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAAt                                                                       >  1:2125118/1‑148 (MQ=255)
                                                                  acCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAAt                                                                       <  1:2855402/148‑1 (MQ=255)
                                                                       gCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACa                                                                  <  1:1252510/148‑1 (MQ=255)
                                                                          tAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATg                                                               >  2:1172606/1‑148 (MQ=255)
                                                                           aaGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGa                                                              >  1:2050475/1‑148 (MQ=255)
                                                                            aGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGaa                                                             <  2:1977035/148‑1 (MQ=255)
                                                                              cAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAAc                                                           >  2:1028603/1‑148 (MQ=255)
                                                                               aataatATTTCGGCGGTAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAAcc                                                          <  2:3077366/148‑1 (MQ=255)
                                                                               aataatATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTTCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAAcc                                                          >  1:3003640/1‑148 (MQ=255)
                                                                                ataataTTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCt                                                         >  2:2848098/1‑148 (MQ=255)
                                                                                  aataTTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGt                                                       >  2:2522868/1‑148 (MQ=255)
                                                                                  aataTTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGt                                                       >  2:967651/1‑148 (MQ=255)
                                                                                  aataTTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGt                                                       <  1:1972588/148‑1 (MQ=255)
                                                                                   atatTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTa                                                      >  1:595115/1‑148 (MQ=255)
                                                                                   atatTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTa                                                      >  1:472288/1‑148 (MQ=255)
                                                                                   atatTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTa                                                      >  1:3563486/1‑148 (MQ=255)
                                                                                     atTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTaaa                                                    >  1:142557/1‑148 (MQ=255)
                                                                                       ttCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAac                                                  >  1:1003792/1‑148 (MQ=255)
                                                                                        tCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAacc                                                 <  1:2518892/148‑1 (MQ=255)
                                                                                            cggGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCt                                             >  1:1127741/1‑148 (MQ=255)
                                                                                               gAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGt                                          <  2:2655107/148‑1 (MQ=255)
                                                                                               gAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGt                                          >  2:1177321/1‑148 (MQ=255)
                                                                                               gAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGt                                          >  1:1529898/1‑148 (MQ=255)
                                                                                                 acacCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTAt                                        <  2:2476251/148‑1 (MQ=255)
                                                                                                     ccTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAAt                                    >  2:2446166/1‑148 (MQ=255)
                                                                                                      cTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATg                                   >  1:3223926/1‑148 (MQ=255)
                                                                                                         cccGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGAc                                >  1:2268596/1‑148 (MQ=255)
                                                                                                         cccGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGAc                                <  1:3216860/148‑1 (MQ=255)
                                                                                                          ccgccgAACTAAAAAATATAGTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACg                               <  2:1739536/148‑1 (MQ=255)
                                                                                                            gccgAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAg                             <  1:2476711/148‑1 (MQ=255)
                                                                                                                aaCTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAGATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTgac                         >  1:1490872/1‑146 (MQ=255)
                                                                                                                aaCTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGcc                         >  2:1366974/1‑148 (MQ=255)
                                                                                                                aaCTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGcc                         >  1:1490422/1‑148 (MQ=255)
                                                                                                                 aCTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCg                        <  2:3026360/148‑1 (MQ=255)
                                                                                                                 aCTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCg                        >  2:2352085/1‑148 (MQ=255)
                                                                                                                  cTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCgg                       >  2:3561487/1‑148 (MQ=255)
                                                                                                                    aaaaaaTATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAATTCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTc                     >  2:3318278/1‑148 (MQ=255)
                                                                                                                    aaaaaaTATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTc                     >  2:2788674/1‑148 (MQ=255)
                                                                                                                          tatatTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCg               >  1:3407181/1‑148 (MQ=255)
                                                                                                                          tatatTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCg               >  1:351082/1‑148 (MQ=255)
                                                                                                                             atTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGca            <  1:1172606/148‑1 (MQ=255)
                                                                                                                             atTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGca            >  2:788683/1‑148 (MQ=255)
                                                                                                                             atTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGGAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGca            >  2:2655537/1‑148 (MQ=255)
                                                                                                                               tCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGcaca          >  2:2168887/1‑148 (MQ=255)
                                                                                                                               tCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGcaca          >  1:1297373/1‑148 (MQ=255)
                                                                                                                               tCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGcaca          >  2:84152/1‑148 (MQ=255)
                                                                                                                                 aaTCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAgg        >  1:162369/1‑148 (MQ=255)
                                                                                                                                     gTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATAAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGc    <  1:2295919/148‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                
TAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGG  >  CP009273/1769625‑1769907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑