Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,796,101 C→A 94.5% D243Y (GAC→TAC)  thrS ← threonyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,796,1010CA94.5% 537.3 / 17.5 191D243Y (GAC→TAC) thrSthreonyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (4/7);  new base A (89/91);  total (93/98)
Fisher's exact test for biased strand distribution p-value = 5.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 194 total aligned reads displayed.

TGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAATG  >  CP009273/1795956‑1796245
                                                                                                                                                 |                                                                                                                                                
tGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTagc                                                                                                                                                >  2:3199119/1‑148 (MQ=255)
 gATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTagcg                                                                                                                                               >  1:217302/1‑148 (MQ=255)
    aCTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGttt                                                                                                                                            <  2:1016116/148‑1 (MQ=255)
      tGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTcg                                                                                                                                          <  2:1579293/148‑1 (MQ=255)
       ggTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTcgc                                                                                                                                         >  1:1481677/1‑148 (MQ=255)
         tACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCgg                                                                                                                                       >  2:3429514/1‑148 (MQ=255)
         tACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCgg                                                                                                                                       >  1:3534956/1‑148 (MQ=255)
            tctTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGctt                                                                                                                                    <  2:1828300/148‑1 (MQ=255)
            tctTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCAAATGGTACAGGTCGAGCTGTTTAACGATTTTACGGTGGTAGCGTTTAGCGGctt                                                                                                                                    >  1:2992556/1‑148 (MQ=255)
             ctTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGcttc                                                                                                                                   >  1:554404/1‑148 (MQ=255)
                tCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCttcttc                                                                                                                                <  1:2469020/148‑1 (MQ=255)
                   gTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAg                                                                                                                             >  2:2129374/1‑148 (MQ=255)
                     ttAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTAACGAATTTACTGTGGTAGCGTTTCGCGGCTTATTACAGgc                                                                                                                           >  2:1985674/1‑148 (MQ=255)
                     ttAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCGTCTTCCTGGATATGGTAAAGGTCGAGCTGTTTAACCATTTTAAGGTGGTAGCGTTTGGCGGCTTGTTCCAGgc                                                                                                                           >  1:3316682/1‑148 (MQ=255)
                      tAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGgcg                                                                                                                          >  2:1010902/1‑148 (MQ=255)
                         aacgaacAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTCCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTg                                                                                                                       >  1:1948175/1‑148 (MQ=255)
                           cgaacAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCa                                                                                                                     <  2:2176135/148‑1 (MQ=255)
                              acaaacaCTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGt                                                                                                                  <  2:597752/148‑1 (MQ=255)
                              acaaacaCTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTAACGATTTTAAGGTGGTATCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGt                                                                                                                  >  1:420373/1‑148 (MQ=255)
                               caaacaCTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCCTATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTa                                                                                                                 >  2:139126/1‑148 (MQ=255)
                                  acacTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGGTGCAGGTAAGc                                                                                                              >  2:2397949/1‑148 (MQ=255)
                                   cacTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCg                                                                                                             <  2:3007728/148‑1 (MQ=255)
                                   cacTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCg                                                                                                             <  2:1231411/148‑1 (MQ=255)
                                   cacTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCg                                                                                                             >  1:763965/1‑148 (MQ=255)
                                    acTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGt                                                                                                            <  2:3137617/148‑1 (MQ=255)
                                    acTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGATGCAGGTAAGCGt                                                                                                            <  1:986015/148‑1 (MQ=255)
                                     cTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTGTTCCTTGATATTGTACAGGTCGAGCTGTTTAACGATTTTAAGGTGGTCGCGTTTCGCGGCGTCGTCCCTGCGCTGCAGGGAAGCggt                                                                                                           >  1:190547/1‑146 (MQ=255)
                                     cTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTTCCGATTTTAAGGTGGTAGCGTTTAGCGGCTTCTTCCAGGCGCGGCAGGTAAGCggg                                                                                                           >  1:3280544/1‑146 (MQ=255)
                                      ttCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTa                                                                                                          >  2:169012/1‑148 (MQ=255)
                                      ttCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCGGCATATGGTACAGGTCGAGCTGTTTAACGATTTTAAGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTa                                                                                                          >  2:965658/1‑148 (MQ=255)
                                        ccAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAg                                                                                                        >  2:1158988/1‑148 (MQ=255)
                                          aGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTg                                                                                                      <  2:76004/148‑1 (MQ=255)
                                            ttCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCt                                                                                                    <  2:240852/148‑1 (MQ=255)
                                             tCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCtt                                                                                                   <  2:1798964/148‑1 (MQ=255)
                                               aCGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCtttt                                                                                                 <  1:352834/148‑1 (MQ=255)
                                                cGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCttttt                                                                                                >  2:845785/1‑148 (MQ=255)
                                                 ggAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCCTATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGGTGAGTGCtttttt                                                                                               >  1:2819984/1‑148 (MQ=255)
                                                 ggAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCtttttt                                                                                               <  1:284288/148‑1 (MQ=255)
                                                  gAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTg                                                                                              <  2:3123952/148‑1 (MQ=255)
                                                  gAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCGGTTTAACGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTTCttttttt                                                                                              >  1:3112194/1‑147 (MQ=255)
                                                   aaGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCttttt                                                                                                >  2:3419126/1‑145 (MQ=255)
                                                   aaGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGt                                                                                             <  2:2727529/148‑1 (MQ=255)
                                                   aaGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGt                                                                                             <  2:2903095/148‑1 (MQ=255)
                                                    aGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTc                                                                                            <  2:989719/148‑1 (MQ=255)
                                                    aGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTc                                                                                            <  2:2009284/148‑1 (MQ=255)
                                                    aGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCAGCATATGGTACAGGTCGAGCTGTTTACAGATTTTAATGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTc                                                                                            >  1:2319416/1‑148 (MQ=255)
                                                     gATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCGTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCt                                                                                           <  2:1104143/148‑1 (MQ=255)
                                                      aTGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTg                                                                                          >  2:1284401/1‑148 (MQ=255)
                                                                     ttGTGCCAGAATACCATACCCGGCGCTTCTTCCGGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGccgtaccgt                                                                           >  2:602997/1‑148 (MQ=255)
                                                                             gAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGt                                                                   <  1:3429514/148‑1 (MQ=255)
                                                                                    ataccCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACa                                                            <  1:1019954/148‑1 (MQ=255)
                                                                                     taccCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACAt                                                           <  2:2676696/148‑1 (MQ=255)
                                                                                      accCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACAtt                                                          <  2:1781642/148‑1 (MQ=255)
                                                                                      accCGGCGCGTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACAtt                                                          <  1:1911985/148‑1 (MQ=255)
                                                                                            cgcTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTttgttg                                                    <  2:190547/148‑1 (MQ=255)
                                                                                            cgcTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTttgttg                                                    >  2:3468084/1‑148 (MQ=255)
                                                                                             gcTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTttgttgt                                                   >  2:351798/1‑148 (MQ=255)
                                                                                              cttcttCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTttgttgtt                                                  >  2:2221270/1‑148 (MQ=255)
                                                                                               ttcttcCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTttgttgttg                                                 >  2:1513080/1‑148 (MQ=255)
                                                                                               ttcttcCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTttgttgttg                                                 >  2:1513212/1‑148 (MQ=255)
                                                                                               ttcttcCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTttgttgttg                                                 >  1:2484237/1‑148 (MQ=255)
                                                                                                tcttcCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGc                                                >  2:2825690/1‑148 (MQ=255)
                                                                                                tcttcCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGc                                                >  1:2361325/1‑148 (MQ=255)
                                                                                                   tcCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGt                                             <  1:2657382/148‑1 (MQ=255)
                                                                                                   tcCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGt                                             <  2:3316682/148‑1 (MQ=255)
                                                                                                      tGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgc                                          <  1:513328/148‑1 (MQ=255)
                                                                                                      tGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgc                                          >  2:649418/1‑148 (MQ=255)
                                                                                                      tGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgc                                          >  2:1117405/1‑148 (MQ=255)
                                                                                                      tGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgc                                          >  2:1118332/1‑148 (MQ=255)
                                                                                                      tGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGGAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgc                                          <  2:2296963/148‑1 (MQ=255)
                                                                                                       gCATATGGTACAGGTCGAGCTGTTTACCGATTATACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgcc                                         >  2:3266485/1‑148 (MQ=255)
                                                                                                        cATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgcca                                        >  1:3060949/1‑148 (MQ=255)
                                                                                                          tatGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgccacg                                      <  1:3162229/148‑1 (MQ=255)
                                                                                                              gTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAg                                  >  2:488740/1‑148 (MQ=255)
                                                                                                               tACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGTTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGt                                 <  1:2397949/148‑1 (MQ=255)
                                                                                                                     tCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGccc                           <  1:2635122/148‑1 (MQ=255)
                                                                                                                     tCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGccc                           <  1:1224191/148‑1 (MQ=255)
                                                                                                                     tCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGccc                           <  1:1114416/148‑1 (MQ=255)
                                                                                                                     tCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGccc                           >  1:267275/1‑148 (MQ=255)
                                                                                                                        aGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTg                        <  1:2166531/148‑1 (MQ=255)
                                                                                                                         gCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGc                       >  1:1707440/1‑148 (MQ=255)
                                                                                                                              ttACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGttt                  >  2:1058509/1‑148 (MQ=255)
                                                                                                                               tACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGtttt                 >  2:1557463/1‑148 (MQ=255)
                                                                                                                                aCCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTc                <  1:1616661/148‑1 (MQ=255)
                                                                                                                                aCCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTc                <  2:542543/148‑1 (MQ=255)
                                                                                                                                aCCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGAGTTAAGTGCTTTTTTGTCAGACCACGCCGTACCGTAAATACGATGCAACATTTTGTTGTTGATGTCGCCACGCCAGTAAGCCCATGCCGTTTTc                >  2:2508867/1‑148 (MQ=255)
                                                                                                                                   gATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCAtt             >  1:2730392/1‑148 (MQ=255)
                                                                                                                                     ttttACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAg           >  2:1868003/1‑148 (MQ=255)
                                                                                                                                     ttttACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAg           <  1:847094/148‑1 (MQ=255)
                                                                                                                                      tttACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTGATTAGt          >  1:3391702/1‑148 (MQ=255)
                                                                                                                                       ttACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGtt         <  2:532520/148‑1 (MQ=255)
                                                                                                                                         aCGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTg       <  2:792521/148‑1 (MQ=255)
                                                                                                                                          cGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGa      <  1:1284401/148‑1 (MQ=255)
                                                                                                                                          cGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTGTTGATTAGTTTgt      >  1:2138215/1‑147 (MQ=255)
                                                                                                                                           ggtggtAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGaa     <  2:3511178/148‑1 (MQ=255)
                                                                                                                                            gtggtAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGaaa    <  2:2899667/148‑1 (MQ=255)
                                                                                                                                            gtggtAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGaaa    <  2:1917615/148‑1 (MQ=255)
                                                                                                                                             tggtAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAat   >  2:2651769/1‑148 (MQ=255)
                                                                                                                                              ggtAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAatg  <  2:555716/148‑1 (MQ=255)
                                                                                                                                              ggtAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAatg  <  2:3386589/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAATG  >  CP009273/1795956‑1796245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑