Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 12,901 G→A 100% D247N (GAT→AAT)  dnaK → chaperone Hsp70, with co‑chaperone DnaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927312,9010GA99.5% 640.1 / ‑4.5 195D247N (GAT→AAT) dnaKchaperone Hsp70, with co‑chaperone DnaJ
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (118/76);  total (119/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 200 total aligned reads displayed.

ACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAAC  >  CP009273/12757‑13045
                                                                                                                                                |                                                                                                                                                
aCTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATc                                                                                                                                               >  1:104155/1‑148 (MQ=255)
aCTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATc                                                                                                                                               >  2:2279230/1‑148 (MQ=255)
 cTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCa                                                                                                                                              >  2:867848/1‑148 (MQ=255)
 cTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCa                                                                                                                                              <  2:3256416/148‑1 (MQ=255)
  tttCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAg                                                                                                                                             <  1:2111316/148‑1 (MQ=255)
  tttCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAg                                                                                                                                             >  2:1397196/1‑148 (MQ=255)
   ttCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAgg                                                                                                                                            <  2:3490421/148‑1 (MQ=255)
    tCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAggg                                                                                                                                           <  1:1532069/148‑1 (MQ=255)
      gATATTTCTATTATCGAAATCGACGAAGTTGACGGAGAAAAAACCTTAGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTCTCTGGTTGAAGAATTCAAGAAAAATCAGGGCa                                                                                                                                         <  1:2030538/148‑1 (MQ=255)
        tatTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCAtt                                                                                                                                       >  2:636932/1‑148 (MQ=255)
        tatTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCAtt                                                                                                                                       <  1:330037/148‑1 (MQ=255)
        tatTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCAtt                                                                                                                                       >  1:23159/1‑148 (MQ=255)
          tttCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGa                                                                                                                                     >  1:1101546/1‑148 (MQ=255)
            tCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCACCTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGAcc                                                                                                                                   <  1:2627010/148‑1 (MQ=255)
            tCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGAcc                                                                                                                                   <  1:3399290/148‑1 (MQ=255)
              tattatCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTg                                                                                                                                 <  1:2509251/148‑1 (MQ=255)
                 tatCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTgcgc                                                                                                                              >  2:925571/1‑148 (MQ=255)
                      aaaTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGa                                                                                                                         >  1:3186657/1‑148 (MQ=255)
                      aaaTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGa                                                                                                                         <  2:2711664/148‑1 (MQ=255)
                      aaaTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGa                                                                                                                         >  1:1241869/1‑148 (MQ=255)
                       aaTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGAt                                                                                                                        <  1:286774/148‑1 (MQ=255)
                        aTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATc                                                                                                                       >  2:2307608/1‑148 (MQ=255)
                        aTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATc                                                                                                                       <  2:531553/148‑1 (MQ=255)
                         tCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATcc                                                                                                                      >  1:3142527/1‑148 (MQ=255)
                          cgacgaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCg                                                                                                                     >  2:2897175/1‑148 (MQ=255)
                          cgacgaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCg                                                                                                                     >  2:2515478/1‑148 (MQ=255)
                           gacgaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGc                                                                                                                    <  2:2626329/148‑1 (MQ=255)
                            acgaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCt                                                                                                                   >  2:577627/1‑148 (MQ=255)
                                aGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGcc                                                                                                               >  1:2300542/1‑147 (MQ=255)
                                aGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCa                                                                                                               <  1:702348/148‑1 (MQ=255)
                                  ttGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAAt                                                                                                             >  1:1308225/1‑148 (MQ=255)
                                   tGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATg                                                                                                            >  1:2372158/1‑148 (MQ=255)
                                   tGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATg                                                                                                            >  2:2194201/1‑148 (MQ=255)
                                   tGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATg                                                                                                            >  2:2194177/1‑148 (MQ=255)
                                     aCGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCa                                                                                                          <  1:146341/148‑1 (MQ=255)
                                         cGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAgcgc                                                                                                      <  2:112022/148‑1 (MQ=255)
                                         cGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAgcgc                                                                                                      <  1:2415022/148‑1 (MQ=255)
                                         cGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAgcgc                                                                                                      >  2:766011/1‑148 (MQ=255)
                                          gAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGcc                                                                                                     <  2:1532366/148‑1 (MQ=255)
                                              aaaCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCACTGCAGCGCCTGaa                                                                                                 >  1:620670/1‑148 (MQ=255)
                                              aaaCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGCGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGaa                                                                                                 <  2:2962169/148‑1 (MQ=255)
                                                aCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAaag                                                                                               <  2:1293396/148‑1 (MQ=255)
                                                  cTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAaagaa                                                                                             >  2:2120461/1‑148 (MQ=255)
                                                   ttCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAaagaag                                                                                            <  2:1834477/148‑1 (MQ=255)
                                                    tCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGc                                                                                           >  1:1003827/1‑148 (MQ=255)
                                                      gAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCgg                                                                                         <  2:2388635/148‑1 (MQ=255)
                                                           tCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGaa                                                                                    >  2:3243356/1‑148 (MQ=255)
                                                              ggCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGaaaaa                                                                                 <  2:1183398/148‑1 (MQ=255)
                                                                caaccaacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGc                                                                               >  2:2872652/1‑148 (MQ=255)
                                                                  accaacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGa                                                                             >  1:1712122/1‑148 (MQ=255)
                                                                   ccaacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGaa                                                                            >  2:100492/1‑148 (MQ=255)
                                                                   ccaacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGaa                                                                            >  2:2317325/1‑148 (MQ=255)
                                                                    caacGGTGATACCCACGTGGGGGGTGAAGACTTCGACAGCCGTCTTATAAAATATCTGGTTGAAGAATTCAATAAAAATCAGGGCATTGACCTGCGCACCGATCCGCTGGAAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGaaa                                                                           <  1:3501887/148‑1 (MQ=255)
                                                                     aacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGaaaa                                                                          >  1:3475217/1‑148 (MQ=255)
                                                                      acGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCACTGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAAt                                                                         >  1:3470130/1‑148 (MQ=255)
                                                                      acGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAAt                                                                         >  1:3134428/1‑148 (MQ=255)
                                                                       cGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATc                                                                        >  1:3564922/1‑148 (MQ=255)
                                                                       cGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATc                                                                        >  1:3519151/1‑148 (MQ=255)
                                                                       cGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATc                                                                        >  1:1757176/1‑148 (MQ=255)
                                                                          tGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGaa                                                                     >  1:2276042/1‑148 (MQ=255)
                                                                          tGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGaa                                                                     <  1:2982282/148‑1 (MQ=255)
                                                                               cccACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTc                                                                <  1:771827/148‑1 (MQ=255)
                                                                                ccACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCt                                                               <  2:120217/148‑1 (MQ=255)
                                                                                  aCCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTc                                                             >  2:480378/1‑148 (MQ=255)
                                                                                       gggggTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTc                                                        >  2:551626/1‑148 (MQ=255)
                                                                                       gggggTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTc                                                        >  2:835385/1‑148 (MQ=255)
                                                                                              aaGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAGGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGAc                                                 >  1:1849133/1‑148 (MQ=255)
                                                                                              aaGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGAc                                                 >  2:1872511/1‑148 (MQ=255)
                                                                                                gACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCg                                               <  2:1132914/148‑1 (MQ=255)
                                                                                                 aCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGa                                              <  2:606673/148‑1 (MQ=255)
                                                                                                 aCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGa                                              >  2:1278899/1‑148 (MQ=255)
                                                                                                  cTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGAc                                             >  2:1714217/1‑148 (MQ=255)
                                                                                                   ttCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACg                                            >  2:1216788/1‑148 (MQ=255)
                                                                                                   ttCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACg                                            <  1:1900423/148‑1 (MQ=255)
                                                                                                     cGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGtt                                          >  1:508562/1‑148 (MQ=255)
                                                                                                      gACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTa                                         <  2:3138086/148‑1 (MQ=255)
                                                                                                       aCAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTaa                                        >  1:2314015/1‑148 (MQ=255)
                                                                                                            cGTCTGATCAACTATCTGGTTTAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGACGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGc                                   <  1:929300/148‑1 (MQ=255)
                                                                                                             gTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGcc                                  >  1:1637752/1‑148 (MQ=255)
                                                                                                               cTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCAt                                >  2:79728/1‑148 (MQ=255)
                                                                                                                   tCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACAt                            >  2:3043506/1‑148 (MQ=255)
                                                                                                                     aaCTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCa                          <  2:2098879/148‑1 (MQ=255)
                                                                                                                      aCTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCAc                         >  2:2966352/1‑148 (MQ=255)
                                                                                                                      aCTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCAc                         >  1:2734311/1‑148 (MQ=255)
                                                                                                                        tATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTg                       >  1:271026/1‑148 (MQ=255)
                                                                                                                         aTCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGc                      <  2:2372158/148‑1 (MQ=255)
                                                                                                                           cTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAg                    >  2:2172160/1‑148 (MQ=255)
                                                                                                                           cTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAg                    <  1:1159991/148‑1 (MQ=255)
                                                                                                                           cTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAg                    <  2:2448508/148‑1 (MQ=255)
                                                                                                                            tGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGa                   >  1:3314712/1‑148 (MQ=255)
                                                                                                                             ggTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGAc                  >  1:2791525/1‑148 (MQ=255)
                                                                                                                             ggTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGAc                  >  2:1999679/1‑148 (MQ=255)
                                                                                                                              gTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGAcg                 <  2:1628722/148‑1 (MQ=255)
                                                                                                                                  aagaaTTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGAc             >  1:823060/1‑148 (MQ=255)
                                                                                                                                    gaaTTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCg           >  2:2553220/1‑148 (MQ=255)
                                                                                                                                     aaTTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCgg          >  1:2184402/1‑148 (MQ=255)
                                                                                                                                        tCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTcc       <  1:130140/148‑1 (MQ=255)
                                                                                                                                           agaaaaaTCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGaa    <  2:3071352/148‑1 (MQ=255)
                                                                                                                                           agaaaaaTCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGaa    <  1:1448062/148‑1 (MQ=255)
                                                                                                                                             aaaaaTCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAac  <  2:1539290/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
ACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAAC  >  CP009273/12757‑13045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑