Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,803,109 C→T 100% A128T (GCC→ACC)  yniB ← putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,803,1090CT95.3% 674.1 / 17.6 214A128T (GCC→ACC) yniBputative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (6/4);  new base T (120/84);  total (126/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 219 total aligned reads displayed.

CCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAA  >  CP009273/1802964‑1803252
                                                                                                                                                 |                                                                                                                                               
 cAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGtttt                                                                                                                                              <  1:3101350/148‑1 (MQ=255)
   aGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGtttttt                                                                                                                                            >  2:1256770/1‑148 (MQ=255)
     cAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTcc                                                                                                                                          >  1:3148614/1‑148 (MQ=255)
     cAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTcc                                                                                                                                          >  2:3287912/1‑148 (MQ=255)
       gagaAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAg                                                                                                                                        >  1:2286330/1‑148 (MQ=255)
        agaAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGa                                                                                                                                       >  2:135485/1‑148 (MQ=255)
        agaAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGa                                                                                                                                       >  1:2957686/1‑148 (MQ=255)
            aaGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGa                                                                                                                                   <  2:3413360/148‑1 (MQ=255)
             aGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAg                                                                                                                                  >  2:3527863/1‑148 (MQ=255)
             aGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAg                                                                                                                                  <  1:2063630/148‑1 (MQ=255)
               aaCACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGtt                                                                                                                                <  1:1125091/148‑1 (MQ=255)
                 caaaTAACCTGCAGCAATAAAAATAACCGGCAGGATATACATTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGa                                                                                                                              <  1:2429965/145‑1 (MQ=255)
                     tAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATCGTATGGTGCGGAACAACAATACGGGCCTCAATTTGTTCACGCGTTGGCCCCTCTTCCCCTGTGGTTTTTGCCAGACGGAGTTgtgctt                                                                                                                          >  2:1933756/1‑143 (MQ=255)
                       aCCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCa                                                                                                                        <  1:1034564/148‑1 (MQ=255)
                        ccTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCaa                                                                                                                       >  1:1980767/1‑148 (MQ=255)
                               caATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTc                                                                                                                >  1:1535640/1‑148 (MQ=255)
                               caATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTc                                                                                                                <  2:1597668/148‑1 (MQ=255)
                               caATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTc                                                                                                                >  2:2676586/1‑148 (MQ=255)
                                aaTACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTcc                                                                                                               <  2:2972204/148‑1 (MQ=255)
                                aaTACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTcc                                                                                                               >  1:3117563/1‑148 (MQ=255)
                                aaTACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTcc                                                                                                               <  2:1314579/148‑1 (MQ=255)
                                     aaaTAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaa                                                                                                          <  2:2840129/148‑1 (MQ=255)
                                       aTAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaaga                                                                                                        <  1:1622158/148‑1 (MQ=255)
                                       aTAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaaga                                                                                                        <  1:1695541/148‑1 (MQ=255)
                                       aTAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaaga                                                                                                        <  2:487437/148‑1 (MQ=255)
                                       aTAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaaga                                                                                                        <  1:3458348/148‑1 (MQ=255)
                                        tAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaagaa                                                                                                       <  1:1803105/148‑1 (MQ=255)
                                        tAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaagaa                                                                                                       >  1:1509033/1‑148 (MQ=255)
                                          aCCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAgc                                                                                                     <  1:2117041/148‑1 (MQ=255)
                                           ccGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAgcg                                                                                                    >  1:526456/1‑148 (MQ=255)
                                             ggCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAgcgcg                                                                                                  <  2:2713971/148‑1 (MQ=255)
                                              gCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAgcgcgc                                                                                                 <  2:2444993/148‑1 (MQ=255)
                                              gCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAgcgcgc                                                                                                 >  1:1909217/1‑148 (MQ=255)
                                               cAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGcc                                                                                                >  1:2826531/1‑148 (MQ=255)
                                                 ggATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTg                                                                                              >  2:2199707/1‑148 (MQ=255)
                                                  gATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTgg                                                                                             >  1:1492338/1‑148 (MQ=255)
                                                  gATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTgg                                                                                             >  1:3046074/1‑148 (MQ=255)
                                                     ataCAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGa                                                                                          >  2:1402602/1‑148 (MQ=255)
                                                     ataCAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGa                                                                                          >  1:2695997/1‑148 (MQ=255)
                                                       aCAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACt                                                                                        >  2:3483120/1‑148 (MQ=255)
                                                        cAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTc                                                                                       >  1:874310/1‑148 (MQ=255)
                                                           tGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATc                                                                                    <  1:3119283/148‑1 (MQ=255)
                                                            gagaAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATcc                                                                                   >  1:1302783/1‑148 (MQ=255)
                                                             agaAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCg                                                                                  <  2:500669/148‑1 (MQ=255)
                                                                aaaaCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGc                                                                               >  1:1423336/1‑148 (MQ=255)
                                                                aaaaCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGc                                                                               <  1:317279/148‑1 (MQ=255)
                                                                  aaCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCAc                                                                             >  1:2091049/1‑148 (MQ=255)
                                                                  aaCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCAc                                                                             >  1:471132/1‑148 (MQ=255)
                                                                    cTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCg                                                                           >  2:892461/1‑148 (MQ=255)
                                                                       cAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGaa                                                                        <  1:2886127/148‑1 (MQ=255)
                                                                         ggAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATc                                                                      <  2:2826531/148‑1 (MQ=255)
                                                                            aaaTAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGACTCCTg                                                                   >  2:3373254/1‑148 (MQ=255)
                                                                               tAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCaa                                                                <  2:3148614/148‑1 (MQ=255)
                                                                                  tATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCACGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGc                                                             >  2:1701689/1‑148 (MQ=255)
                                                                                   aTGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGcc                                                            >  2:3561084/1‑148 (MQ=255)
                                                                                    tGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCa                                                           >  1:1618660/1‑148 (MQ=255)
                                                                                          ggtacaacaATACGGGTCTCAATTTGTTCAAGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGa                                                     <  1:1376507/145‑1 (MQ=255)
                                                                                          ggAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGa                                                     <  1:1758787/148‑1 (MQ=255)
                                                                                           gAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGAc                                                    >  2:6518/1‑148 (MQ=255)
                                                                                                acaaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACaaaaa                                               >  1:2346717/1‑148 (MQ=255)
                                                                                                 caaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCCGTCCGACAAAAAt                                              >  1:1039119/1‑148 (MQ=255)
                                                                                                 caaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAAt                                              <  2:2905253/148‑1 (MQ=255)
                                                                                                 caaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAAt                                              >  1:1760899/1‑148 (MQ=255)
                                                                                                  aaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATc                                             <  1:767975/148‑1 (MQ=255)
                                                                                                    tACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAg                                           >  1:92925/1‑148 (MQ=255)
                                                                                                     aCGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAgg                                          <  2:2237491/148‑1 (MQ=255)
                                                                                                     aCGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAgg                                          <  1:2171771/148‑1 (MQ=255)
                                                                                                     aCGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAgg                                          <  1:2768583/148‑1 (MQ=255)
                                                                                                      cGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGa                                         <  2:1996087/148‑1 (MQ=255)
                                                                                                        ggACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATg                                       >  2:732679/1‑148 (MQ=255)
                                                                                                          aCTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGaa                                     >  1:963096/1‑148 (MQ=255)
                                                                                                           cTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGaaa                                    >  1:1113226/1‑148 (MQ=255)
                                                                                                              aaTTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATc                                 <  2:2264609/148‑1 (MQ=255)
                                                                                                                tttGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCAc                               >  1:2647515/1‑148 (MQ=255)
                                                                                                                tttGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGAGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCAc                               >  2:2274623/1‑148 (MQ=255)
                                                                                                                 ttGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCAcc                              <  2:1909217/148‑1 (MQ=255)
                                                                                                                 ttGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCAcc                              >  1:327463/1‑148 (MQ=255)
                                                                                                                  tGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCAccc                             <  2:2957686/148‑1 (MQ=255)
                                                                                                                     tCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGa                          >  1:1884589/1‑148 (MQ=255)
                                                                                                                     tCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGa                          >  1:81773/1‑148 (MQ=255)
                                                                                                                          cgTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATa                     <  2:478839/148‑1 (MQ=255)
                                                                                                                          cgTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATa                     <  2:759963/148‑1 (MQ=255)
                                                                                                                           gTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATaa                    <  1:347391/148‑1 (MQ=255)
                                                                                                                           gTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATaa                    >  1:333402/1‑148 (MQ=255)
                                                                                                                           gTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATaa                    <  1:2531401/148‑1 (MQ=255)
                                                                                                                             tAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACg                  >  1:3378603/1‑148 (MQ=255)
                                                                                                                             tAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACg                  >  2:2762210/1‑148 (MQ=255)
                                                                                                                              aGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGt                 <  2:3056659/148‑1 (MQ=255)
                                                                                                                               gCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGtt                >  2:1639051/1‑148 (MQ=255)
                                                                                                                                ccccTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTc               >  2:1071884/1‑148 (MQ=255)
                                                                                                                                ccccTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACAACTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATTAAAATAACCCACAACATAAAGTTc               >  1:2141607/1‑148 (MQ=255)
                                                                                                                                     ctTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGcc          >  2:875087/1‑148 (MQ=255)
                                                                                                                                      ttCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGcca         >  1:645943/1‑148 (MQ=255)
                                                                                                                                         cccTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccacca      <  1:136340/148‑1 (MQ=255)
                                                                                                                                         cccTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccacca      <  1:1097683/148‑1 (MQ=255)
                                                                                                                                          ccTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCAt     <  1:3561084/148‑1 (MQ=255)
                                                                                                                                           cTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCAtt    >  2:1450277/1‑148 (MQ=255)
                                                                                                                                           cTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCAtt    >  2:2633641/1‑148 (MQ=255)
                                                                                                                                           cTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCAtt    >  1:628228/1‑148 (MQ=255)
                                                                                                                                             ttGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTaa  >  1:1159277/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAA  >  CP009273/1802964‑1803252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑