BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,839,854	C→A	63.4%	R455L (CGC→CTC)	topB ←	DNA topoisomerase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,839,854	0	C	A	63.4%	58.0 / 210.1	195	R455L (CGC→CTC)	topB	DNA topoisomerase III
Reads supporting (aligned to +/- strand): ref base C (32/38); new base A (60/64); total (93/102)
Fisher's exact test for biased strand distribution p-value = 7.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.25e-02

Only 100 of 201 total aligned reads displayed.

TCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAA  >  CP009273/1839711‑1840001

 cccGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGctct                                                                                                                                                  >  2:1750320/1‑148 (MQ=255)

 cccGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTAATCCAGctcg                                                                                                                                                  >  1:739793/1‑147 (MQ=255)

 cccGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTATTCATCGAGctct                                                                                                                                                  >  1:894303/1‑148 (MQ=255)

  ccGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCtt                                                                                                                                                 <  2:1873288/148‑1 (MQ=255)

     gTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTCAAAGCGCGCttttg                                                                                                                                              >  1:1600689/1‑146 (MQ=255)

     gTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTtgc                                                                                                                                              <  1:815847/148‑1 (MQ=255)

     gTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTtgc                                                                                                                                              <  2:3237722/148‑1 (MQ=255)

     gTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTtgc                                                                                                                                              <  2:3237285/148‑1 (MQ=255)

     gTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTAATCGCGCTCttttc                                                                                                                                              >  1:1450311/1‑146 (MQ=255)

                  gCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGt                                                                                                                                 >  2:361184/1‑148 (MQ=255)

                   cAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTCATCGCGCTCTTTTGTGGCTAACAGCGTg                                                                                                                                >  1:147997/1‑148 (MQ=255)

                   cAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTGTTGATCGAGCTCTGTTGTGCCGATCAGCGTg                                                                                                                                >  1:1022295/1‑148 (MQ=255)

                        ttGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCa                                                                                                                           >  2:1321670/1‑148 (MQ=255)

                            aTCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTGTTGATCGGGGGCTTTTGTGCCTAAAAGCGTGcgcccgccg                                                                                                                       >  1:1682818/1‑147 (MQ=255)

                             tCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTg                                                                                                                      <  2:744319/148‑1 (MQ=255)

                              cGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGc                                                                                                                     >  2:145821/1‑148 (MQ=255)

                              cGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGc                                                                                                                     <  2:2932594/148‑1 (MQ=255)

                              cGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGc                                                                                                                     <  1:2223653/148‑1 (MQ=255)

                              cGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGc                                                                                                                     <  1:1126304/148‑1 (MQ=255)

                                  aaaaTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCa                                                                                                                 >  2:2132839/1‑148 (MQ=255)

                                      tGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCaa                                                                                                             >  1:2603241/1‑148 (MQ=255)

                                       ggCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCCGCCGGCGTCAGccac                                                                                                            >  2:642032/1‑145 (MQ=255)

                                          gcggcgGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGaaa                                                                                                         <  1:419403/148‑1 (MQ=255)

                                          gcggcgGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGaaa                                                                                                         <  1:279232/148‑1 (MQ=255)

                                            ggcggcTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCCCAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAc                                                                                                       <  2:3304984/148‑1 (MQ=255)

                                             gcggcTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAcg                                                                                                      >  2:1092079/1‑148 (MQ=255)

                                              cggcTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAcgc                                                                                                     <  2:608291/148‑1 (MQ=255)

                                                gcTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTTCTGCCTAAAAGCGTGCGCCAGCCTGCTTAAGCAAGAAAAAgcgc                                                                                                   >  1:1171040/1‑148 (MQ=255)

                                                gcTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTATTAATAGAGCTATTTGATGCCGAAAACCGTGCGCCCCCCAGCTTAAGCAACAAAACacgc                                                                                                   >  1:548060/1‑148 (MQ=255)

                                                gcTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTGTCTTCATCGAGCTCTTTGGTGCCTAACAGCGTGCGCCACCCAGCTTCAGCAACAAAAccccc                                                                                                   >  1:413994/1‑144 (MQ=255)

                                                 cTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCt                                                                                                  <  1:3461435/148‑1 (MQ=255)

                                                 cTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCt                                                                                                  <  2:2353574/148‑1 (MQ=255)

                                                 cTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTAATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCt                                                                                                  <  1:1753433/148‑1 (MQ=255)

                                                   gggTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCttt                                                                                                <  1:2241095/148‑1 (MQ=255)

                                                   gggTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTATTCATAGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAAGCGCttt                                                                                                >  2:87784/1‑148 (MQ=255)

                                                    ggTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTAATACCCCTCATTTCTGCCAAAAAACGTGCGCCAGCCAGCTTAAGCAAAAAAAATCGCATTa                                                                                               >  1:3056689/1‑148 (MQ=255)

                                                     gTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAg                                                                                              <  1:2541456/148‑1 (MQ=255)

                                                     gTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTCTTCATCGAGCTCTTTGCTGCCTAACAGCCAGCGGCCGCCAGCTTAACCAAGAAAAAGCGCATTat                                                                                              >  2:1210242/1‑147 (MQ=255)

                                                      tttGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGc                                                                                             >  2:173172/1‑148 (MQ=255)

                                                        tGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCCCAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGa                                                                                           <  2:580995/148‑1 (MQ=255)

                                                            gCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑Ca‑aa                                                                                     <  2:1577698/148‑1 (MQ=255)

                                                             ctctACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AAt                                                                                    <  2:1055615/148‑1 (MQ=255)

                                                             ctctACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AAt                                                                                    >  2:105788/1‑148 (MQ=255)

                                                              tctACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTGTTAAAACAGCTCTTTTATGCCTAACAGCGTGCGCCAGCCAGCTTCAACCAGAAAACCCGCGTTAGCgcccaaat                                                                                     >  1:3438044/1‑141 (MQ=255)

                                                                  ccACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTgac                                                                               <  2:41015/148‑3 (MQ=255)

                                                                   cACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCt                                                                              <  1:2067315/148‑1 (MQ=255)

                                                                   cACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCt                                                                              <  1:2957106/148‑1 (MQ=255)

                                                                   cACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCt                                                                              <  1:2873092/148‑1 (MQ=255)

                                                                   cACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCt                                                                              <  1:2872891/148‑1 (MQ=255)

                                                                    aCTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTTCCtt                                                                             >  2:2588445/1‑148 (MQ=255)

                                                                    aCTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGGGCGCCCGCCTGCGTCAGCAAGAAAACGCGCTTTAGCGA‑CC‑AATTTGCCtt                                                                             >  2:3499864/1‑148 (MQ=255)

                                                                       tCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTAATCGAGCTCTTTTCTGCCTAACAGCGTGCGCCACCCGGCTTAAGCAAGAAAAAACGCGTTAGAGACCA‑AAtttaattttg                                                                           >  1:943144/1‑141 (MQ=255)

                                                                       tCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTgg                                                                          <  2:3085919/148‑1 (MQ=255)

                                                                         aCCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAAGCGCTTTATCGA‑CA‑AATTTTCCGTTGGCa                                                                        >  1:2967676/1‑148 (MQ=255)

                                                                         aCCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATAGAGCTCATTTCTGCCGAAAAGCGTGCGCCAGCCTGCTTCAGCCAGAAAACGCGCGTTATCGA‑CA‑AAATTTCCTTTTGCa                                                                        >  1:1961219/1‑148 (MQ=255)

                                                                         aCCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTCTTCATCGAGCTCTTTTCTGCCGAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAAGCGCTTTAGCGA‑CA‑AAATTTCCTTTggga                                                                        >  1:1494733/1‑146 (MQ=255)

                                                                           cTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTGTTCATCGCGCTCGTTGCTGCCTAACAGCGTGCGCCAGCCGGCTTCAGCAAGAAAAAGCGCGTTAGCGA‑CA‑AAATTTACGTTTGCAAt                                                                      >  1:1091822/1‑148 (MQ=255)

                                                                             ttttCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGt                                                                    <  2:167954/148‑1 (MQ=255)

                                                                             ttttCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGt                                                                    <  2:168938/148‑1 (MQ=255)

                                                                               ttCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTCTTCATCGAGCTCTTTGGTGCCGAACAGCGTGCGCCCGCCGGCGTCAGCCAGAAAACGCGCTTTAGCGA‑CACAATTTCCGTTTggactgtcc                                                                   >  1:2203513/1‑141 (MQ=255)

                                                                                  acacaGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGt                                                               <  2:2228460/148‑1 (MQ=255)

                                                                                   cacaGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGtt                                                              <  2:664220/148‑1 (MQ=255)

                                                                                        cAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTGTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATa                                                         <  1:1256876/148‑1 (MQ=255)

                                                                                          aCTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAac                                                       <  2:1085958/148‑1 (MQ=255)

                                                                                              aTCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAacacac                                                   <  1:3236916/148‑1 (MQ=255)

                                                                                                cGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACtt                                                 <  2:112636/148‑1 (MQ=255)

                                                                                                 gCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTg                                                <  1:2664371/148‑1 (MQ=255)

                                                                                                  ccTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGc                                               <  1:3540694/148‑1 (MQ=255)

                                                                                                    tttCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCgg                                             >  2:2877507/1‑148 (MQ=255)

                                                                                                      tCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGaa                                           <  1:3152860/148‑1 (MQ=255)

                                                                                                        gCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTCAACGCGCTCGTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCGTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTGGATAAAACACATTCGGAaca                                         >  2:929013/1‑148 (MQ=255)

                                                                                                        gCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAaca                                         <  1:343271/148‑1 (MQ=255)

                                                                                                            ccacAGGCAGTGGCGTGCCGTCGTGTTCTTCATCGAGCTCTTTGCTGCCTAAAAGCCTGCGCCAGCCGGCGTCAGCAAGAAAACCCCCTTTAGCGA‑CA‑AAATTTCCATTGGCCATGTGCAGTTCGATAACACACTTTCGGAACAcccc                                     >  1:3257965/1‑146 (MQ=255)

                                                                                                             cacaGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCa                                    <  1:490731/148‑1 (MQ=255)

                                                                                                             cacaGGCAGTGGCGTGCCGTCGTGTTCTTAATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCCGCCTGCTTCAGCCAGAAAACGCGCGTTAGCGA‑CA‑AATTTGCCTTTGGCCATGTCCAGTTCGATAACACACTTGCGGAACACCCCa                                    >  2:187702/1‑148 (MQ=255)

                                                                                                                aGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATcc                                 <  1:3114969/148‑1 (MQ=255)

                                                                                                                aGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCATTGGCAATGTCCAGTTCGATAACACACATTCGGAACACCGCATcc                                 >  2:586362/1‑148 (MQ=255)

                                                                                                                 ggCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCg                                <  1:2132494/148‑1 (MQ=255)

                                                                                                                 ggCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCg                                <  2:3696/148‑1 (MQ=255)

                                                                                                                 ggCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCg                                <  1:610417/148‑1 (MQ=255)

                                                                                                                    aGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGc                             <  2:1082788/148‑1 (MQ=255)

                                                                                                                      tGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCGTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑ACTTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAg                           <  2:2596033/148‑1 (MQ=255)

                                                                                                                             ccGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCa                    <  2:2667969/148‑1 (MQ=255)

                                                                                                                               gtcgtTTTCTTCATAGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAAGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTTCGGAACACCGCATCCGGGCAGAATTGCATc                  >  1:3424620/1‑148 (MQ=255)

                                                                                                                                tcgtTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCa                 >  2:77676/1‑148 (MQ=255)

                                                                                                                                tcgtTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCa                 >  2:2470052/1‑148 (MQ=255)

                                                                                                                                     ttcttcATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATAc            <  2:2759756/148‑1 (MQ=255)

                                                                                                                                      tcttcATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACt           >  2:707341/1‑148 (MQ=255)

                                                                                                                                       cttcATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTg          <  2:312214/148‑1 (MQ=255)

                                                                                                                                       cttcATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTg          <  1:173587/148‑1 (MQ=255)

                                                                                                                                       cttcATCGAGCTCTTTGCTGCCTAACTGAGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACGGCATCCGGGCAGAATTGCATCAGATTCTg          <  2:1961520/148‑1 (MQ=255)

                                                                                                                                        ttcATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGa         <  1:642032/148‑1 (MQ=255)

                                                                                                                                         tcATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGAc        >  1:498165/1‑148 (MQ=255)

                                                                                                                                         tcATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGAc        >  2:3358035/1‑148 (MQ=255)

                                                                                                                                         tcATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGAc        >  1:1294351/1‑148 (MQ=255)

                                                                                                                                          cATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACg       <  2:1450311/148‑1 (MQ=255)

                                                                                                                                            tCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACggg     >  2:1158884/1‑148 (MQ=255)

                                                                                                                                               cgcTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCaa  <  1:2913937/148‑1 (MQ=255)

                                                                                                                                               cgcTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCaa  <  1:145821/148‑1 (MQ=255)

                                                                                                                                               cgcTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCaa  >  2:2715208/1‑148 (MQ=255)

TCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAA  >  CP009273/1839711‑1840001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`