Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 166,662 C→A 100% S133* (TCG→TAG)  fhuC → iron‑hydroxamate transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273166,6620CA95.3% 562.1 / 16.6 192S133* (TCG→TAG) fhuCiron‑hydroxamate transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (5/4);  new base A (96/87);  total (101/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 194 total aligned reads displayed.

CGCAAAGTGGCTTATTTGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCA  >  CP009273/166517‑166809
                                                                                                                                                 |                                                                                                                                                   
cGCAAAGTGGCTTATTTGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGc                                                                                                                                                   >  1:16106/1‑148 (MQ=255)
  cAAAGTGGCTTATTTGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTg                                                                                                                                                 <  1:3308478/148‑1 (MQ=255)
  cAAAGTGGCTTATTTGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTg                                                                                                                                                 <  1:3414059/148‑1 (MQ=255)
       tGGCTTATTTGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTgg                                                                                                                                            <  2:1590246/148‑1 (MQ=255)
        ggCTTATTTGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGc                                                                                                                                           <  1:1509574/148‑1 (MQ=255)
        ggCTTATTTGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGc                                                                                                                                           >  2:283951/1‑148 (MQ=255)
        ggCTTATTTGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGc                                                                                                                                           >  1:1845199/1‑148 (MQ=255)
          cTTATTTGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCtt                                                                                                                                         >  2:1173122/1‑148 (MQ=255)
            tATTTGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTaa                                                                                                                                       >  1:529396/1‑148 (MQ=255)
            tATTTGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTaa                                                                                                                                       >  2:2473666/1‑148 (MQ=255)
               ttGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAAc                                                                                                                                    <  2:661681/148‑1 (MQ=255)
               ttGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAAc                                                                                                                                    >  1:1672477/1‑148 (MQ=255)
                tGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAAcc                                                                                                                                   >  1:3480613/1‑148 (MQ=255)
                 gCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCg                                                                                                                                  <  1:186229/148‑1 (MQ=255)
                    gcagcaGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTg                                                                                                                               >  1:3438731/1‑148 (MQ=255)
                          gcTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCAt                                                                                                                         <  2:2034155/148‑1 (MQ=255)
                           cTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATc                                                                                                                        >  2:2155739/1‑148 (MQ=255)
                           cTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATc                                                                                                                        >  2:359562/1‑148 (MQ=255)
                            ttCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCg                                                                                                                       >  2:1253761/1‑148 (MQ=255)
                            ttCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCg                                                                                                                       >  2:1253918/1‑148 (MQ=255)
                             tcctccGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCgg                                                                                                                      >  2:888826/1‑148 (MQ=255)
                             tcctccGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCgg                                                                                                                      >  2:1858921/1‑148 (MQ=255)
                             tcctccGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCgg                                                                                                                      >  2:54496/1‑148 (MQ=255)
                             tcctccGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCgg                                                                                                                      <  1:1734903/148‑1 (MQ=255)
                              cctccGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGc                                                                                                                     >  2:698919/1‑148 (MQ=255)
                              cctccGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGc                                                                                                                     >  2:2201941/1‑148 (MQ=255)
                              cctccGGCAGAAGGGATGACCGTGCGTGAACTGGTGGAGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCAATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGc                                                                                                                     >  2:911203/1‑148 (MQ=255)
                                 ccGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTgg                                                                                                                  <  1:848578/148‑1 (MQ=255)
                                       gAAGGGATGACCGTGCGTGAACTGGTGGCGAGTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCCGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATa                                                                                                            <  1:2192150/148‑1 (MQ=255)
                                          gggATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGtc                                                                                                         >  1:445528/1‑148 (MQ=255)
                                          gggATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGtc                                                                                                         >  1:2820702/1‑148 (MQ=255)
                                             aTGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGtctct                                                                                                      >  2:1949375/1‑148 (MQ=255)
                                             aTGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGtctct                                                                                                      >  1:1752243/1‑148 (MQ=255)
                                                aCCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTg                                                                                                   >  2:2423702/1‑148 (MQ=255)
                                                 ccGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTgg                                                                                                  >  2:1516576/1‑148 (MQ=255)
                                                   gtgcgtgAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTggcg                                                                                                >  1:3378261/1‑148 (MQ=255)
                                                      cgtgAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGgcggcg                                                                                             >  1:1028575/1‑148 (MQ=255)
                                                        tgAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGaa                                                                                           >  2:792418/1‑148 (MQ=255)
                                                        tgAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGaa                                                                                           >  1:495261/1‑148 (MQ=255)
                                                          aaCTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACg                                                                                         >  1:2079926/1‑148 (MQ=255)
                                                            cTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTc                                                                                       >  2:1236672/1‑148 (MQ=255)
                                                            cTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTc                                                                                       >  1:2030462/1‑148 (MQ=255)
                                                             tggtggCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCa                                                                                      >  2:2328041/1‑148 (MQ=255)
                                                                 ggCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGCTAGTCTCTCTGGCGGCGACCGTCAGCgg                                                                                  >  2:3196076/1‑148 (MQ=255)
                                                                 ggCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCgg                                                                                  >  2:2688759/1‑148 (MQ=255)
                                                                      ttGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTg                                                                             >  1:1112995/1‑148 (MQ=255)
                                                                      ttGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTg                                                                             <  1:861956/148‑1 (MQ=255)
                                                                        ggTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCTAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGa                                                                           <  1:699794/148‑1 (MQ=255)
                                                                            gTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGc                                                                       <  1:1236672/148‑1 (MQ=255)
                                                                             ttACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGcc                                                                      <  1:2007818/148‑1 (MQ=255)
                                                                                cccGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATg                                                                   <  2:959664/148‑1 (MQ=255)
                                                                                cccGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATg                                                                   >  2:3470006/1‑148 (MQ=255)
                                                                                 ccGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGc                                                                  >  2:3141356/1‑148 (MQ=255)
                                                                                 ccGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGc                                                                  <  1:1272063/148‑1 (MQ=255)
                                                                                    tGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCtgg                                                               <  2:161922/148‑1 (MQ=255)
                                                                                              cgcTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGa                                                     >  1:2288051/1‑148 (MQ=255)
                                                                                                   gggCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGACGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGcc                                                >  2:2972252/1‑148 (MQ=255)
                                                                                                   gggCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGcc                                                >  1:682327/1‑148 (MQ=255)
                                                                                                   gggCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGcc                                                <  1:1298291/148‑1 (MQ=255)
                                                                                                    ggCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCg                                               <  1:3075904/148‑1 (MQ=255)
                                                                                                     gcgcTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGt                                              >  1:823190/1‑148 (MQ=255)
                                                                                                        cTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGt                                           <  1:735335/148‑1 (MQ=255)
                                                                                                         tttGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTc                                          <  2:2224483/148‑1 (MQ=255)
                                                                                                          ttGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCt                                         <  1:1971013/148‑1 (MQ=255)
                                                                                                          ttGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCt                                         <  1:989517/148‑1 (MQ=255)
                                                                                                           tGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTg                                        <  1:3522542/148‑1 (MQ=255)
                                                                                                           tGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTg                                        <  1:1106710/148‑1 (MQ=255)
                                                                                                            ggggCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGt                                       <  1:3289057/148‑1 (MQ=255)
                                                                                                              ggcggcAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTg                                     <  1:1253761/148‑1 (MQ=255)
                                                                                                              ggcggcAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTg                                     >  1:1713663/1‑148 (MQ=255)
                                                                                                              ggcggcAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTg                                     <  2:529396/148‑1 (MQ=255)
                                                                                                               gcggcAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGc                                    >  2:3134673/1‑148 (MQ=255)
                                                                                                                   cAGATCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTcga                                >  2:799152/1‑148 (MQ=255)
                                                                                                                      aTCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTcgacga                             >  2:1327315/1‑148 (MQ=255)
                                                                                                                      aTCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTcgacga                             <  1:3102744/148‑1 (MQ=255)
                                                                                                                      aTCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTcgacga                             <  2:16106/148‑1 (MQ=255)
                                                                                                                      aTCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTcgacga                             >  2:3100339/1‑148 (MQ=255)
                                                                                                                       tCGCGAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGaa                            <  1:2900668/148‑1 (MQ=255)
                                                                                                                          cgAAAAAGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCg                         >  1:1605161/1‑148 (MQ=255)
                                                                                                                            aaaaaGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGAc                       >  1:2059828/1‑148 (MQ=255)
                                                                                                                            aaaaaGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGAc                       >  1:1931771/1‑148 (MQ=255)
                                                                                                                            aaaaaGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGAc                       <  1:796765/148‑1 (MQ=255)
                                                                                                                             aaaaGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGAcc                      >  2:2707733/1‑148 (MQ=255)
                                                                                                                             aaaaGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGAcc                      <  1:3242627/148‑1 (MQ=255)
                                                                                                                              aaaGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATGGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGACCCGACCt                     >  2:2295817/1‑148 (MQ=255)
                                                                                                                              aaaGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCt                     >  2:419609/1‑148 (MQ=255)
                                                                                                                                aGTCGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCg                   >  1:526745/1‑148 (MQ=255)
                                                                                                                                   cGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGgcg                <  1:930017/148‑1 (MQ=255)
                                                                                                                                   cGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGgcg                <  2:3341043/148‑1 (MQ=255)
                                                                                                                                   cGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGgcg                <  1:2427283/148‑1 (MQ=255)
                                                                                                                                   cGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGgcg                >  1:2522056/1‑148 (MQ=255)
                                                                                                                                   cGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGgcg                <  1:2126905/148‑1 (MQ=255)
                                                                                                                                   cGAGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGgcg                <  1:362004/148‑1 (MQ=255)
                                                                                                                                     aGGAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCt              >  1:1872519/1‑148 (MQ=255)
                                                                                                                                       gAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTgg            <  2:823266/148‑1 (MQ=255)
                                                                                                                                       gAAGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTgg            <  1:2201974/148‑1 (MQ=255)
                                                                                                                                         aGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGat          <  1:2959121/148‑1 (MQ=255)
                                                                                                                                         aGCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGat          <  1:2577745/148‑1 (MQ=255)
                                                                                                                                          gCTATCTAGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGata         >  1:2384623/1‑148 (MQ=255)
                                                                                                                                                 cGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATCGCcca  <  1:715959/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CGCAAAGTGGCTTATTTGCCGCAGCAGCTTCCTCCGGCAGAAGGGATGACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTTGGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGCTTAAAACCGCTGGCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATGCTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATCGCCCA  >  CP009273/166517‑166809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑