Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,870,743 C→A 100% H272N (CAT→AAT)  yeaN → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,870,7430CA97.7% 537.2 / 3.7 175H272N (CAT→AAT) yeaNputative transporter
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (89/82);  total (91/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 176 total aligned reads displayed.

CTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGG  >  CP009273/1870598‑1870884
                                                                                                                                                 |                                                                                                                                             
cTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTAcataat                                                                                                                                             <  1:608162/148‑1 (MQ=255)
      ggTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGaaa                                                                                                                                       >  2:1448547/1‑148 (MQ=255)
      ggTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGaaa                                                                                                                                       >  2:3197688/1‑148 (MQ=255)
       gTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAg                                                                                                                                      >  1:284621/1‑148 (MQ=255)
         cTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGAt                                                                                                                                    >  2:1144827/1‑148 (MQ=255)
         cTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGAt                                                                                                                                    <  1:3463434/148‑1 (MQ=255)
           aTTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCa                                                                                                                                  >  2:2604658/1‑148 (MQ=255)
            ttACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAg                                                                                                                                 <  1:2904801/148‑1 (MQ=255)
              aCGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCg                                                                                                                               <  1:2543615/148‑1 (MQ=255)
              aCGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCg                                                                                                                               >  1:2455724/1‑148 (MQ=255)
               cGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGt                                                                                                                              >  2:238892/1‑148 (MQ=255)
                 tGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCAGGGTGTGCTGCAACTAGCCACAGCAGCACCCGGGTTGCTGATCCCACTTTTCTTACATATTGTGAAAGATCAGCGTgg                                                                                                                            >  2:147776/1‑148 (MQ=255)
                   attattGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCACACTTTTCTTACATAATGTGAAAGATCAGCGTGGTa                                                                                                                          >  2:482238/1‑148 (MQ=255)
                    ttattGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTAt                                                                                                                         <  2:1752140/148‑1 (MQ=255)
                     tattGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTAtt                                                                                                                        >  2:2717157/1‑148 (MQ=255)
                       ttGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGc                                                                                                                      <  2:1702398/148‑1 (MQ=255)
                           ctggctTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGcg                                                                                                                  <  2:1969312/148‑1 (MQ=255)
                           ctggctTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGcg                                                                                                                  <  2:297001/148‑1 (MQ=255)
                             ggctTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGcgtt                                                                                                                >  2:2268196/1‑148 (MQ=255)
                              gctTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGcgttc                                                                                                               >  2:3243460/1‑148 (MQ=255)
                              gctTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGcgttc                                                                                                               >  2:1974841/1‑148 (MQ=255)
                              gctTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGcgttc                                                                                                               >  2:1541046/1‑148 (MQ=255)
                                 tCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGcgttcgtt                                                                                                            >  1:857197/1‑148 (MQ=255)
                                  ccGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTg                                                                                                           <  1:536356/148‑1 (MQ=255)
                                   cGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGc                                                                                                          <  2:3390508/148‑1 (MQ=255)
                                    ggCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGcc                                                                                                         <  2:3152303/148‑1 (MQ=255)
                                         tCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGAt                                                                                                    >  2:132466/1‑148 (MQ=255)
                                          ccTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCAGGTTTGCTGATCCCACTTTTCTGACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGAtg                                                                                                   <  1:1450612/148‑1 (MQ=255)
                                           cTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGAtgt                                                                                                  <  1:2043215/148‑1 (MQ=255)
                                             catcaGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTgc                                                                                                >  1:727568/1‑148 (MQ=255)
                                             catcaGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTgc                                                                                                <  2:3562815/148‑1 (MQ=255)
                                               tcaGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTgcgc                                                                                              >  1:100916/1‑148 (MQ=255)
                                               tcaGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTgcgc                                                                                              >  2:793787/1‑148 (MQ=255)
                                                caGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCa                                                                                             >  1:3120387/1‑148 (MQ=255)
                                                  gTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGt                                                                                           >  2:1279689/1‑148 (MQ=255)
                                                  gTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGt                                                                                           <  2:3210139/148‑1 (MQ=255)
                                                    cACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTgg                                                                                         >  2:849687/1‑148 (MQ=255)
                                                     aCGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTggg                                                                                        >  1:510482/1‑148 (MQ=255)
                                                     aCGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTggg                                                                                        <  1:3371528/148‑1 (MQ=255)
                                                     aCGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTggg                                                                                        <  1:3371520/148‑1 (MQ=255)
                                                       ggCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGgcg                                                                                      <  1:2735612/148‑1 (MQ=255)
                                                          tataGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTGTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCgg                                                                                   <  1:1203629/148‑1 (MQ=255)
                                                           ataGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGt                                                                                  >  1:1520465/1‑148 (MQ=255)
                                                           ataGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGt                                                                                  <  2:3511427/148‑1 (MQ=255)
                                                              gCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTgg                                                                               >  1:2501754/1‑148 (MQ=255)
                                                                   gCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGctct                                                                          >  2:1318055/1‑148 (MQ=255)
                                                                       aGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCtt                                                                      <  2:3512755/148‑1 (MQ=255)
                                                                       aGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCtt                                                                      >  2:711433/1‑148 (MQ=255)
                                                                            ggTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGc                                                                 <  2:3119152/148‑1 (MQ=255)
                                                                            ggTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGc                                                                 <  1:542471/148‑1 (MQ=255)
                                                                                 aCTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAgcg                                                            <  2:2975681/148‑1 (MQ=255)
                                                                                   tGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCa                                                          >  1:2347947/1‑148 (MQ=255)
                                                                                        ggTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGa                                                     <  1:1350321/148‑1 (MQ=255)
                                                                                        ggTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGa                                                     >  1:2294134/1‑148 (MQ=255)
                                                                                            tgctgcAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCAc                                                 <  1:2372311/148‑1 (MQ=255)
                                                                                                gcAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTgg                                             <  2:630464/148‑1 (MQ=255)
                                                                                                gcAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTgg                                             <  2:199325/148‑1 (MQ=255)
                                                                                                 cAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGa                                            >  2:500611/1‑148 (MQ=255)
                                                                                                 cAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGa                                            >  1:1970667/1‑148 (MQ=255)
                                                                                                     tAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGActct                                        >  2:2575680/1‑148 (MQ=255)
                                                                                                       gCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGc                                      <  2:2528394/148‑1 (MQ=255)
                                                                                                        ccACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCt                                     >  1:3222581/1‑148 (MQ=255)
                                                                                                           cagcagCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCtttt                                  >  2:85832/1‑148 (MQ=255)
                                                                                                             gcagcaCCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCg                                <  1:2447628/148‑1 (MQ=255)
                                                                                                              cagcaCCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCgg                               <  2:1047121/148‑1 (MQ=255)
                                                                                                              cagcaCCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCgg                               <  2:2501754/148‑1 (MQ=255)
                                                                                                              cagcaCCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCgg                               >  1:2230436/1‑148 (MQ=255)
                                                                                                                gcaCCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGtt                             >  2:2494475/1‑148 (MQ=255)
                                                                                                                gcaCCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGtt                             >  1:555116/1‑148 (MQ=255)
                                                                                                                   cccGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTg                          >  2:2611891/1‑148 (MQ=255)
                                                                                                                   cccGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTg                          >  2:882228/1‑148 (MQ=255)
                                                                                                                     cGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGt                        <  1:2608254/148‑1 (MQ=255)
                                                                                                                     cGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGt                        <  2:408255/148‑1 (MQ=255)
                                                                                                                       gTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGATTTTCGGTTTTGGTTc                      <  1:2717157/148‑1 (MQ=255)
                                                                                                                        tttGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTcc                     <  2:3020537/148‑1 (MQ=255)
                                                                                                                        tttGCTGATCCCACTGTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTcc                     >  1:6653/1‑148 (MQ=255)
                                                                                                                        tttGCTGATCCCACTGTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTcc                     >  1:7063/1‑148 (MQ=255)
                                                                                                                          tGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCgg                   >  2:1091173/1‑148 (MQ=255)
                                                                                                                             tGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGgcgc                >  2:468640/1‑148 (MQ=255)
                                                                                                                              gATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGca               >  1:2389172/1‑148 (MQ=255)
                                                                                                                              gATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGca               >  1:1419409/1‑148 (MQ=255)
                                                                                                                              gATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGca               >  2:2326859/1‑148 (MQ=255)
                                                                                                                               aTCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaa              <  1:944763/148‑1 (MQ=255)
                                                                                                                               aTCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaa              >  1:528383/1‑148 (MQ=255)
                                                                                                                               aTCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaa              <  2:3381115/148‑1 (MQ=255)
                                                                                                                                tCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaac             >  2:3385381/1‑148 (MQ=255)
                                                                                                                                tCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaac             >  2:664242/1‑148 (MQ=255)
                                                                                                                                 cccACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaaca            <  1:238892/148‑1 (MQ=255)
                                                                                                                                   cACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAAt          >  1:1000343/1‑148 (MQ=255)
                                                                                                                                    aCTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATg         >  1:345265/1‑148 (MQ=255)
                                                                                                                                     cTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGa        >  1:352769/1‑148 (MQ=255)
                                                                                                                                     cTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGa        >  1:2149312/1‑148 (MQ=255)
                                                                                                                                      ttttCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGAt       >  2:2291420/1‑148 (MQ=255)
                                                                                                                                      ttttCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGAt       <  2:2445697/148‑1 (MQ=255)
                                                                                                                                      ttttCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGAt       <  2:2587465/148‑1 (MQ=255)
                                                                                                                                        ttCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATAc     <  1:1540563/148‑1 (MQ=255)
                                                                                                                                          cTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTg   <  1:1950348/148‑1 (MQ=255)
                                                                                                                                          cTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTg   <  1:370484/148‑1 (MQ=255)
                                                                                                                                           ttACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTgg  <  2:1688124/148‑1 (MQ=255)
                                                                                                                                           ttACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTgg  <  2:2808657/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
CTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGG  >  CP009273/1870598‑1870884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑