Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 168,584 A→T 43.4% L212F (TTA→TTT)  fhuB → fused iron‑hydroxamate transporter subunits of ABC superfamily: membrane components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273168,5840AT43.4% 107.3 / 284.5 207L212F (TTA→TTT) fhuBfused iron‑hydroxamate transporter subunits of ABC superfamily: membrane components
Reads supporting (aligned to +/- strand):  ref base A (51/66);  new base T (44/46);  total (95/112)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 208 total aligned reads displayed.

ACTGGTTATCTTCCATCATGACCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTATCGGCTTTATCGGGTTGTTCGC  >  CP009273/168437‑168730
                                                                                                                                                   |                                                                                                                                                  
  tGGTTATCTTCCATCATGACCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAAc                                                                                                                                                  <  1:2596788/148‑1 (MQ=255)
   ggTTATCTTCCATCATGACCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTAcc                                                                                                                                                 <  1:2280457/148‑1 (MQ=255)
    gTTATCTTCCATCATGACCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAAccc                                                                                                                                                <  2:2849920/148‑1 (MQ=255)
      tATCTTCCATCATGACCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTg                                                                                                                                              >  1:3026525/1‑148 (MQ=255)
       aTCTTCCATCATGACCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGa                                                                                                                                             <  1:1568727/148‑1 (MQ=255)
        tCTTCCATCATGACCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGAt                                                                                                                                            <  2:1246416/148‑1 (MQ=255)
            ccATCATGACCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATgggg                                                                                                                                        <  1:456695/148‑1 (MQ=255)
             catcatGACCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGc                                                                                                                                       <  2:2031888/148‑1 (MQ=255)
              atcatGACCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCt                                                                                                                                      >  2:1625624/1‑148 (MQ=255)
              atcatGACCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCt                                                                                                                                      <  1:3489092/148‑1 (MQ=255)
              atcatGACCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCt                                                                                                                                      <  1:3488723/148‑1 (MQ=255)
               tcatGACCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCtt                                                                                                                                     <  2:1706775/148‑1 (MQ=255)
               tcatGACCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCtt                                                                                                                                     <  2:3470440/148‑1 (MQ=255)
                catGACCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTtg                                                                                                                                    <  2:1737802/148‑1 (MQ=255)
                catGACCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTtg                                                                                                                                    <  2:571014/148‑1 (MQ=255)
                catGACCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTtg                                                                                                                                    <  2:640923/148‑1 (MQ=255)
                    aCCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTtgatga                                                                                                                                <  1:1087899/148‑1 (MQ=255)
                    aCCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTtgatga                                                                                                                                >  1:310768/1‑148 (MQ=255)
                    aCCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTtgatga                                                                                                                                <  2:843550/148‑1 (MQ=255)
                    aCCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTtgatga                                                                                                                                <  1:1105221/148‑1 (MQ=255)
                    aCCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTtgatga                                                                                                                                <  1:685424/148‑1 (MQ=255)
                      cAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGatgatg                                                                                                                              >  1:588894/1‑148 (MQ=255)
                        aCTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTGTGGCCGCCGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGAGGGGGCTTGATGAtggc                                                                                                                            >  1:905405/1‑148 (MQ=255)
                        aCTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGAtggc                                                                                                                            >  1:2686823/1‑148 (MQ=255)
                          tGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGTCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGAtggcgt                                                                                                                          >  1:3384466/1‑148 (MQ=255)
                            cAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGAtggcgtgg                                                                                                                        >  1:427141/1‑148 (MQ=255)
                              aaGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGAtggcgtggcg                                                                                                                      >  1:414880/1‑148 (MQ=255)
                               aGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGgcgc                                                                                                                     >  1:3539242/1‑148 (MQ=255)
                                gCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGgcgcg                                                                                                                    >  1:474484/1‑148 (MQ=255)
                                 cATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGgcgcgc                                                                                                                   >  1:2652037/1‑148 (MQ=255)
                                     tttCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAAtc                                                                                                               >  1:2619072/1‑148 (MQ=255)
                                                cACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGcc                                                                                                    <  1:1522027/148‑1 (MQ=255)
                                                cACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGcc                                                                                                    <  1:1522077/148‑1 (MQ=255)
                                                cACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGcc                                                                                                    <  2:1000671/148‑1 (MQ=255)
                                                    ggAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGt                                                                                                <  1:1005596/148‑1 (MQ=255)
                                                       aCGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGc                                                                                             <  2:730636/148‑1 (MQ=255)
                                                        cGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCt                                                                                            <  1:1929263/148‑1 (MQ=255)
                                                            gACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTgcg                                                                                        >  2:1450275/1‑148 (MQ=255)
                                                             aCGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTgcgc                                                                                       >  2:1846673/1‑148 (MQ=255)
                                                             aCGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCACAATCTCGGGCTGGCCTTGTCGCTTgcgc                                                                                       >  1:1746042/1‑148 (MQ=255)
                                                              cGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGcgcg                                                                                      >  1:1886362/1‑148 (MQ=255)
                                                                  aaCCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTg                                                                                  <  2:1284223/148‑1 (MQ=255)
                                                                  aaCCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTg                                                                                  <  2:2604975/148‑1 (MQ=255)
                                                                   aCCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTgg                                                                                 <  1:503527/148‑1 (MQ=255)
                                                                   aCCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTgg                                                                                 <  1:504508/148‑1 (MQ=255)
                                                                    ccGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGc                                                                                >  2:3541233/1‑148 (MQ=255)
                                                                      gACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAg                                                                              <  2:1447533/148‑1 (MQ=255)
                                                                              cggcgTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCg                                                                      >  1:794144/1‑148 (MQ=255)
                                                                              cggcgTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCg                                                                      >  1:2582091/1‑148 (MQ=255)
                                                                              cggcgTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCg                                                                      <  1:1660330/148‑1 (MQ=255)
                                                                               ggcgTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGc                                                                     <  2:2125608/148‑1 (MQ=255)
                                                                                gcgTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACATCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCt                                                                    >  2:2466541/1‑148 (MQ=255)
                                                                                   ttGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGc                                                                 >  2:791030/1‑148 (MQ=255)
                                                                                     gAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGa                                                               >  1:1376269/1‑148 (MQ=255)
                                                                                     gAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGa                                                               <  2:3183967/148‑1 (MQ=255)
                                                                                     gAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGa                                                               <  2:3332585/148‑1 (MQ=255)
                                                                                           ttATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGtca                                                         >  1:58995/1‑148 (MQ=255)
                                                                                           ttATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGtca                                                         >  2:2791868/1‑148 (MQ=255)
                                                                                           ttATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGtca                                                         <  2:3025872/148‑1 (MQ=255)
                                                                                           ttATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGtca                                                         >  2:1234663/1‑148 (MQ=255)
                                                                                           ttATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGtca                                                         <  1:1839017/148‑1 (MQ=255)
                                                                                           ttATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGtca                                                         >  2:3343896/1‑148 (MQ=255)
                                                                                            tATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGtcat                                                        <  1:3197949/148‑1 (MQ=255)
                                                                                            tATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGtcat                                                        <  2:1557121/148‑1 (MQ=255)
                                                                                              tGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGtcatca                                                      >  2:1286027/1‑148 (MQ=255)
                                                                                              tGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGtcatca                                                      >  2:1285877/1‑148 (MQ=255)
                                                                                               ggCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAg                                                     >  2:2682541/1‑148 (MQ=255)
                                                                                                    cAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTgcgc                                                <  2:2092802/148‑1 (MQ=255)
                                                                                                      gctgctGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCgctg                                              >  2:2861870/1‑148 (MQ=255)
                                                                                                          ctgGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTgg                                          <  1:1308723/148‑1 (MQ=255)
                                                                                                          ctgGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTgg                                          <  1:3473322/148‑1 (MQ=255)
                                                                                                                  tgtgATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCt                                  >  2:599228/1‑148 (MQ=255)
                                                                                                                       tGCTGACGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTggg                             >  1:2094636/1‑148 (MQ=255)
                                                                                                                       tGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTggg                             <  1:431077/148‑1 (MQ=255)
                                                                                                                        gCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTgggg                            >  2:817738/1‑148 (MQ=255)
                                                                                                                            aCGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTa                        >  2:1320208/1‑148 (MQ=255)
                                                                                                                            aCGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTa                        >  1:189698/1‑148 (MQ=255)
                                                                                                                            aCGTTGCTGCTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTa                        <  1:2861870/148‑1 (MQ=255)
                                                                                                                            aCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTa                        <  2:3001455/148‑1 (MQ=255)
                                                                                                                                tgctgcTACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTATCgg                    <  2:3255927/148‑1 (MQ=255)
                                                                                                                                  ctgctACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTATCGGCt                  <  1:1229823/148‑1 (MQ=255)
                                                                                                                                  ctgctACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTATCGGCt                  <  2:427141/148‑1 (MQ=255)
                                                                                                                                  ctgctACTTCGTCCGTGAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTATCGGCt                  <  1:1931489/148‑1 (MQ=255)
                                                                                                                                   tgctACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCGGGCGATTTTGATCAGTGCGGTGCAGGTGGACGCGGTGGGGAGTATAGGGtt                 >  1:603310/1‑148 (MQ=255)
                                                                                                                                      tACTTCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTATCGGCTTTAt              <  2:677599/148‑1 (MQ=255)
                                                                                                                                      tACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTATCGGCTTTAt              <  1:32930/148‑1 (MQ=255)
                                                                                                                                      tACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTATCGGCTTTAt              >  1:2389575/1‑148 (MQ=255)
                                                                                                                                       aCTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGGCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTATCGGCTTTATc             >  2:1811381/1‑148 (MQ=255)
                                                                                                                                          tCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTATCGGCTTTATCggg          <  2:2953794/148‑1 (MQ=255)
                                                                                                                                          tCGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTATCGGCTTTATCggg          <  1:829953/148‑1 (MQ=255)
                                                                                                                                           cGTCCGTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTATCGGCTTTATCGGgt         <  1:2466541/148‑1 (MQ=255)
                                                                                                                                             tCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTATCGGCTTTATCGGgttg       <  2:147782/148‑1 (MQ=255)
                                                                                                                                              ccGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTATCGGCTTTATCGGgttgt      <  2:837009/148‑1 (MQ=255)
                                                                                                                                              ccGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTATCGGCTTTATCGGgttgt      <  1:1282115/148‑1 (MQ=255)
                                                                                                                                              ccGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTATCGGCTTTATCGGgttgt      <  2:264445/148‑1 (MQ=255)
                                                                                                                                                gTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTATCGGCTTTATCGGGTTGTTc    >  1:2963776/1‑148 (MQ=255)
                                                                                                                                                gTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTATCGGCTTTATCGGGTTGTTc    >  1:1600232/1‑148 (MQ=255)
                                                                                                                                                gTTTACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTATCGGCTTTATCGGGTTGTTc    >  1:1325337/1‑148 (MQ=255)
                                                                                                                                                  tAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTATCGGCTTTATCGGGTTGTTcgc  >  2:2353931/1‑148 (MQ=255)
                                                                                                                                                  tAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTATCGGCTTTATCGGGTTGTTcgc  >  2:2555432/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACTGGTTATCTTCCATCATGACCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTGGTGAACGCTGTGGGGATTATCGGCTTTATCGGGTTGTTCGC  >  CP009273/168437‑168730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑