Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,904,418 C→A 15.5% intergenic (‑62/‑115) kdgR ← / → yebQ KDG regulon transcriptional repressor/putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,904,4180CA15.5% 582.9 / 57.0 194intergenic (‑62/‑115)kdgR/yebQKDG regulon transcriptional repressor/putative transporter
Reads supporting (aligned to +/- strand):  ref base C (85/78);  new base A (16/15);  total (101/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 198 total aligned reads displayed.

TCTTCACCCAGCGCCTGCAAAATGCCAAAAACTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTG  >  CP009273/1904271‑1904565
                                                                                                                                                   |                                                                                                                                                   
   tCACCCAGCGCCAGCAAAATGCCAAAAACTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACAAgg                                                                                                                                                  <  2:810362/148‑1 (MQ=255)
    cACCCAGCGCCTGCAAAATGCCAAAAACTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAggg                                                                                                                                                 <  1:758864/148‑1 (MQ=255)
            gcCTGCAAAATGCCAAAAACTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCa                                                                                                                                         <  1:472985/148‑1 (MQ=255)
            gcCTGCAAAATGCCAAAAACTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCAGTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCa                                                                                                                                         <  1:1271980/148‑1 (MQ=255)
               tGCAAAATGCCAAAAACTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATc                                                                                                                                      >  1:3040923/1‑148 (MQ=255)
                gCAAAATGCCAAAAACTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATcc                                                                                                                                     >  2:1330230/1‑148 (MQ=255)
                    aaTGCCAAAAACTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACAAGGGTGGTCGCAATCCATCt                                                                                                                                 >  1:3317784/1‑148 (MQ=255)
                     aTGCCAAAAACTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCtt                                                                                                                                >  1:2988943/1‑148 (MQ=255)
                     aTGCCAAAAACTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCtt                                                                                                                                >  1:1591632/1‑148 (MQ=255)
                      tGCCAAAAACTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACAAGGGTGGTCGCACTCCATCttt                                                                                                                               >  1:1314850/1‑148 (MQ=255)
                       gCCAAAAACTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCtttt                                                                                                                              >  2:862329/1‑148 (MQ=255)
                          aaaaaCTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGcc                                                                                                                           >  2:137390/1‑148 (MQ=255)
                           aaaaCTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCg                                                                                                                          >  1:2383134/1‑148 (MQ=255)
                            aaaCTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACAAGGGTGGTCGCAATCCATCTTTTGCCgg                                                                                                                         >  2:1043578/1‑148 (MQ=255)
                             aaCTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGgt                                                                                                                        <  1:2848432/148‑1 (MQ=255)
                             aaCTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACAAGGGTGGTCGCAATCCATCTTTTGCCGgt                                                                                                                        <  2:2550647/148‑1 (MQ=255)
                                ttttAGCACGGAAGATACAGAATCAGGCTGTTTATCCATATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGAGGTCGCAATCCATCTTTTTCCGgttag                                                                                                                     >  2:2017622/1‑148 (MQ=255)
                                ttttAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGgttag                                                                                                                     <  2:3176746/148‑1 (MQ=255)
                                 tttAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACAAGGGTGGTCGCAATCCATCTTTTGCCGgttagt                                                                                                                    >  1:1028009/1‑148 (MQ=255)
                                   tAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGgttagtta                                                                                                                  <  1:2136200/148‑1 (MQ=255)
                                       aCGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAAt                                                                                                              >  1:3019864/1‑148 (MQ=255)
                                        cGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAAtt                                                                                                             <  2:2197153/148‑1 (MQ=255)
                                         ggAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTc                                                                                                            >  2:3092115/1‑148 (MQ=255)
                                           aaGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTg                                                                                                          <  1:3076422/148‑1 (MQ=255)
                                           aaGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTg                                                                                                          <  1:2393678/148‑1 (MQ=255)
                                             gATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCg                                                                                                        >  2:1602220/1‑148 (MQ=255)
                                             gATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCg                                                                                                        >  1:2623447/1‑148 (MQ=255)
                                              aTACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGa                                                                                                       >  2:2335973/1‑148 (MQ=255)
                                                  aGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATc                                                                                                   <  1:1281372/148‑1 (MQ=255)
                                                  aGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATc                                                                                                   <  1:3440393/148‑1 (MQ=255)
                                                  aGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATc                                                                                                   <  1:1281213/148‑1 (MQ=255)
                                                          gCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGa                                                                                           >  2:1795437/1‑148 (MQ=255)
                                                           cTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGaa                                                                                          <  1:3310461/148‑1 (MQ=255)
                                                            tGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAat                                                                                         <  2:2219514/148‑1 (MQ=255)
                                                             gTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAata                                                                                        <  2:1540044/148‑1 (MQ=255)
                                                              tttATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAatat                                                                                       <  1:1794585/148‑1 (MQ=255)
                                                              tttATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAatat                                                                                       >  2:1180764/1‑148 (MQ=255)
                                                               ttATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATc                                                                                      <  2:23883/148‑1 (MQ=255)
                                                                tATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCa                                                                                     <  1:1342339/148‑1 (MQ=255)
                                                                tATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCa                                                                                     <  2:157137/148‑1 (MQ=255)
                                                                  tCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGt                                                                                   >  2:3496066/1‑148 (MQ=255)
                                                                   ccAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTg                                                                                  <  1:1424472/148‑1 (MQ=255)
                                                                     aGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCt                                                                                >  2:703238/1‑148 (MQ=255)
                                                                         cTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGaa                                                                            >  1:3285647/1‑148 (MQ=255)
                                                                         cTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGaa                                                                            >  1:359951/1‑148 (MQ=255)
                                                                         cTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACAAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGaa                                                                            <  2:2863901/148‑1 (MQ=255)
                                                                         cTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTAATATAATTTCGCACCAGGGTTGTCGCAAACCATCTATTGACGGTTAGTTACAATTCTGCGAAATCAACCCTTAATATAAGTGCTAGaa                                                                            >  1:3286028/1‑148 (MQ=255)
                                                                          tGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAAt                                                                           >  2:668097/1‑148 (MQ=255)
                                                                           gCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATc                                                                          >  1:3256447/1‑148 (MQ=255)
                                                                               tAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATAc                                                                      >  1:3487759/1‑148 (MQ=255)
                                                                               tAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATAc                                                                      >  1:642044/1‑148 (MQ=255)
                                                                               tAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACAAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATAc                                                                      >  2:2958350/1‑148 (MQ=255)
                                                                                    aTTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACAAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTg                                                                 <  2:1972333/148‑1 (MQ=255)
                                                                                     tttATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGt                                                                <  2:2822100/148‑1 (MQ=255)
                                                                                     tttATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGt                                                                >  2:1662394/1‑148 (MQ=255)
                                                                                        aTCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGa                                                             <  2:3142978/148‑1 (MQ=255)
                                                                                        aTCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACAAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGa                                                             >  1:28528/1‑148 (MQ=255)
                                                                                          cACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGatt                                                           <  1:615749/148‑1 (MQ=255)
                                                                                          cACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGatt                                                           <  2:3461564/148‑1 (MQ=255)
                                                                                          cACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGatt                                                           >  2:981070/1‑148 (MQ=255)
                                                                                           aCCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGatta                                                          >  1:571954/1‑148 (MQ=255)
                                                                                             cTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGattatt                                                        <  2:2151147/148‑1 (MQ=255)
                                                                                              tCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTc                                                       <  1:2632491/148‑1 (MQ=255)
                                                                                               cATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCa                                                      >  1:2740837/1‑148 (MQ=255)
                                                                                                 ttGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCAcc                                                    <  2:184699/148‑1 (MQ=255)
                                                                                                 ttGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTNGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCAcc                                                    >  1:1811267/1‑148 (MQ=255)
                                                                                                  tGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCa                                                   >  2:1162590/1‑148 (MQ=255)
                                                                                                  tGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATACACCa                                                   >  2:1162546/1‑148 (MQ=255)
                                                                                                   gCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCaa                                                  <  1:857860/148‑1 (MQ=255)
                                                                                                      aGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGa                                               <  1:2680702/148‑1 (MQ=255)
                                                                                                       gtgtTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGATAGAATCATACCCCTGTTGATTATTCACCAAAGat                                              >  2:1489070/1‑148 (MQ=255)
                                                                                                              ataAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAAt                                       <  2:1428413/148‑1 (MQ=255)
                                                                                                               taAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAAtt                                      <  1:2440971/148‑1 (MQ=255)
                                                                                                                 aaaaTCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTcc                                    <  2:3040923/148‑1 (MQ=255)
                                                                                                                  aaaTCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTCTTCACCAAAGATATAAAATTCCt                                   >  2:3537816/1‑148 (MQ=255)
                                                                                                                  aaaTCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCt                                   <  2:210875/148‑1 (MQ=255)
                                                                                                                  aaaTCAGAACTGTTTTTTATTATAATTTCGCACAAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCt                                   <  1:1622422/148‑1 (MQ=255)
                                                                                                                     tCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATg                                <  1:3409364/148‑1 (MQ=255)
                                                                                                                     tCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAAATCCTATg                                >  2:1855424/1‑148 (MQ=255)
                                                                                                                      cAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATAATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGc                               >  2:560346/1‑148 (MQ=255)
                                                                                                                              ttttttATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGtt                       >  2:2965647/1‑148 (MQ=255)
                                                                                                                               tttttATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTc                      <  2:2887504/148‑1 (MQ=255)
                                                                                                                               tttttATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTc                      >  1:1909508/1‑148 (MQ=255)
                                                                                                                               tttttATTATAATTTCGCACAAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTc                      <  2:976909/148‑1 (MQ=255)
                                                                                                                                    attatAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGcc                 <  1:862329/148‑1 (MQ=255)
                                                                                                                                    attatAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGcc                 >  1:20191/1‑148 (MQ=255)
                                                                                                                                     ttatAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAATGATATAAAATTCCTATGCCAAAAGTTCAGGCCg                >  2:2300293/1‑148 (MQ=255)
                                                                                                                                     ttatAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCg                >  1:1694938/1‑148 (MQ=255)
                                                                                                                                        taATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACg             >  2:502087/1‑148 (MQ=255)
                                                                                                                                         aaTTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCGGTTGATTAGTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACgg            <  2:3140723/148‑1 (MQ=255)
                                                                                                                                         aaTTTCGCACAAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACgg            >  1:2142014/1‑148 (MQ=255)
                                                                                                                                           tttCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGcc          <  1:451844/148‑1 (MQ=255)
                                                                                                                                           tttCGCACAAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGcc          <  1:1043578/148‑1 (MQ=255)
                                                                                                                                            ttCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCt         >  1:251757/1‑148 (MQ=255)
                                                                                                                                            ttCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCt         >  1:538767/1‑148 (MQ=255)
                                                                                                                                            ttCGCACAAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCt         >  2:1883160/1‑148 (MQ=255)
                                                                                                                                             tCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTg        >  1:1099925/1‑148 (MQ=255)
                                                                                                                                                cACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCa     <  1:2335866/148‑1 (MQ=255)
                                                                                                                                                 aCCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCAt    >  2:2615260/1‑148 (MQ=255)
                                                                                                                                                  ccAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCAtt   <  1:423088/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCTTCACCCAGCGCCTGCAAAATGCCAAAAACTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTG  >  CP009273/1904271‑1904565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑