Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,922,164 C→A 68.0% P311P (CCG→CCT)  ptrB ← protease II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,922,1640CA68.0% 203.5 / 240.7 225P311P (CCG→CCT) ptrBprotease II
Reads supporting (aligned to +/- strand):  ref base C (35/37);  new base A (78/75);  total (113/112)
Fisher's exact test for biased strand distribution p-value = 7.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 229 total aligned reads displayed.

AGGCCGGATCATCAAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAA  >  CP009273/1922019‑1922309
                                                                                                                                                 |                                                                                                                                                 
aGGCCGGATCATCAAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCgg                                                                                                                                                 <  1:3308220/148‑1 (MQ=255)
aGGCCGGATCATCAAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAgg                                                                                                                                                 >  1:354226/1‑148 (MQ=255)
 ggCCGGATCATCAAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTggagga                                                                                                                                                <  1:384730/148‑1 (MQ=255)
  gCCGGATCATCAAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGaa                                                                                                                                               <  2:1036074/148‑1 (MQ=255)
   ccGGATCATCAAAGGCCATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGCTAACCCGCGCTGACGCTCTTCGACCACCAGCCAGTCGGTAAACAGCGGAAACCCGTCCAGCTTGATGGTTTCGCGTggaggact                                                                                                                                              >  2:2612651/1‑146 (MQ=255)
    cGGATCATCAAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAAtt                                                                                                                                             <  1:812722/148‑1 (MQ=255)
      gATCATCAAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTaa                                                                                                                                           <  2:2700850/148‑1 (MQ=255)
       atcatcAAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAAc                                                                                                                                          >  2:2834622/1‑148 (MQ=255)
       atcatcAAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAAc                                                                                                                                          <  1:498736/148‑1 (MQ=255)
        tcatcaAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAAct                                                                                                                                         <  1:3040421/148‑1 (MQ=255)
            caAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTc                                                                                                                                     <  1:3235201/148‑1 (MQ=255)
               aGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCa                                                                                                                                  <  2:2121524/148‑1 (MQ=255)
               aGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCa                                                                                                                                  <  2:560619/148‑1 (MQ=255)
               aGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCa                                                                                                                                  >  2:3557195/1‑148 (MQ=255)
               aGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCa                                                                                                                                  <  2:450429/148‑1 (MQ=255)
               aGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCa                                                                                                                                  >  1:1939474/1‑148 (MQ=255)
                          ctGACTTCCCGGGTCTTGCGGTTAATTTGGCGCACACTGGTTACCCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCtcat                                                                                                                       <  2:1905732/147‑1 (MQ=255)
                               ttCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGc                                                                                                                  <  2:3274701/148‑1 (MQ=255)
                                tCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCa                                                                                                                 >  1:2387890/1‑148 (MQ=255)
                                tCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCa                                                                                                                 >  1:2753531/1‑148 (MQ=255)
                                   cGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATAc                                                                                                              <  1:3181366/148‑1 (MQ=255)
                                   cGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATAc                                                                                                              >  1:1902743/1‑148 (MQ=255)
                                       tCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGt                                                                                                          >  2:6245/1‑148 (MQ=255)
                                        cTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTa                                                                                                         <  1:3486467/148‑1 (MQ=255)
                                          tGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACg                                                                                                       >  1:51961/1‑148 (MQ=255)
                                          tGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACg                                                                                                       <  2:1108787/148‑1 (MQ=255)
                                             ggTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGta                                                                                                    <  2:1694758/148‑1 (MQ=255)
                                             ggTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGCTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGta                                                                                                    <  2:1694544/148‑1 (MQ=255)
                                               ttAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGtata                                                                                                  <  2:1408328/148‑1 (MQ=255)
                                                        cgcAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGt                                                                                         <  2:354226/148‑1 (MQ=255)
                                                             aCTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTg                                                                                    >  1:1506817/1‑148 (MQ=255)
                                                              cTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGc                                                                                   >  2:313604/1‑148 (MQ=255)
                                                                  ttAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTg                                                                               <  1:366023/148‑1 (MQ=255)
                                                                   tAACCCGCGCTGACGCTCTTCAACCACCCGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTgg                                                                              <  1:2941573/148‑1 (MQ=255)
                                                                     aCCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCg                                                                            >  2:812259/1‑148 (MQ=255)
                                                                     aCCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCg                                                                            <  1:2491814/148‑1 (MQ=255)
                                                                      cccGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCgg                                                                           >  1:972008/1‑148 (MQ=255)
                                                                      cccGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCgg                                                                           <  2:3283750/148‑1 (MQ=255)
                                                                      cccGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCgg                                                                           <  1:1379638/148‑1 (MQ=255)
                                                                       ccGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGt                                                                          <  2:3382245/148‑1 (MQ=255)
                                                                       ccGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGt                                                                          <  1:3557195/148‑1 (MQ=255)
                                                                          cgcTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTgg                                                                       <  1:2439037/148‑1 (MQ=255)
                                                                            cTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGaa                                                                     <  1:1762161/148‑1 (MQ=255)
                                                                            cTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGaa                                                                     <  1:3143837/148‑1 (MQ=255)
                                                                            cTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGaa                                                                     <  1:2688882/148‑1 (MQ=255)
                                                                                  ctctTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGa                                                               <  1:3326951/148‑1 (MQ=255)
                                                                                   tctTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGAt                                                              <  1:3008968/148‑1 (MQ=255)
                                                                                   tctTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGAt                                                              <  1:3494161/148‑1 (MQ=255)
                                                                                    ctTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATa                                                             >  2:1481628/1‑148 (MQ=255)
                                                                                     ttCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATaa                                                            <  2:901272/148‑1 (MQ=255)
                                                                                      tCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATaaa                                                           >  1:537363/1‑148 (MQ=255)
                                                                                        aaCCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATaaaaa                                                         >  2:1977193/1‑148 (MQ=255)
                                                                                          ccaccaGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACg                                                       <  1:929705/148‑1 (MQ=255)
                                                                                          ccaccaGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACg                                                       >  2:2752644/1‑148 (MQ=255)
                                                                                          ccaccaGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACg                                                       <  1:1607351/148‑1 (MQ=255)
                                                                                               agccagTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCt                                                  >  1:69370/1‑148 (MQ=255)
                                                                                               agccagTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCt                                                  >  1:1873705/1‑148 (MQ=255)
                                                                                                gccagTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTg                                                 >  2:3173401/1‑148 (MQ=255)
                                                                                                 ccagTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTgg                                                <  1:3216002/148‑1 (MQ=255)
                                                                                                    gTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAg                                             >  1:545572/1‑148 (MQ=255)
                                                                                                     tCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGt                                            >  1:594259/1‑148 (MQ=255)
                                                                                                      cGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTg                                           >  2:116959/1‑148 (MQ=255)
                                                                                                      cGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTg                                           >  1:1078056/1‑148 (MQ=255)
                                                                                                       ggTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGa                                          <  1:1663000/148‑1 (MQ=255)
                                                                                                        gTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTGCCCACTGTTGCTCATCACGCATCGGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCATATAAAAACGATGCTGGTAGTGAt                                         <  1:2116/148‑1 (MQ=255)
                                                                                                          aaaCAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCa                                       >  1:1047128/1‑148 (MQ=255)
                                                                                                          aaaCAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCa                                       <  1:226013/148‑1 (MQ=255)
                                                                                                           aaCAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCaa                                      <  1:433373/148‑1 (MQ=255)
                                                                                                             cAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAgg                                    <  1:418107/148‑1 (MQ=255)
                                                                                                              aGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGc                                   <  1:845101/148‑1 (MQ=255)
                                                                                                              aGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGc                                   <  2:2553952/148‑1 (MQ=255)
                                                                                                                cGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTg                                 >  1:1749743/1‑148 (MQ=255)
                                                                                                                cGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTg                                 >  1:1163728/1‑148 (MQ=255)
                                                                                                                cGTAAACCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTg                                 >  1:2205151/1‑148 (MQ=255)
                                                                                                                 gTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGt                                <  1:267251/148‑1 (MQ=255)
                                                                                                                  tAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTa                               >  1:2913824/1‑148 (MQ=255)
                                                                                                                   aaaCCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTAt                              >  1:2532199/1‑148 (MQ=255)
                                                                                                                    aaCCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTAtt                             >  1:74286/1‑148 (MQ=255)
                                                                                                                    aaCCCTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTAtt                             >  1:1209894/1‑148 (MQ=255)
                                                                                                                      cccTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCACAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCg                           <  2:2375716/148‑1 (MQ=255)
                                                                                                                      cccTTCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCg                           >  2:1182167/1‑148 (MQ=255)
                                                                                                                       ccTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGt                          >  2:2884776/1‑148 (MQ=255)
                                                                                                                        cTTCCATCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGTTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTg                         <  1:2609199/148‑1 (MQ=255)
                                                                                                                          tCCAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGAt                       >  1:2076265/1‑148 (MQ=255)
                                                                                                                           ccAGCATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATc                      >  2:3521218/1‑148 (MQ=255)
                                                                                                                               cATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTg                  >  2:2235228/1‑148 (MQ=255)
                                                                                                                               cATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTg                  >  2:39053/1‑148 (MQ=255)
                                                                                                                               cATGATGTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTg                  <  1:3521218/148‑1 (MQ=255)
                                                                                                                                 tgatgTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTgcg                >  2:524802/1‑148 (MQ=255)
                                                                                                                                  gatgTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTgcgg               >  2:1792937/1‑148 (MQ=255)
                                                                                                                                  gatgTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTgcgg               >  2:1235974/1‑148 (MQ=255)
                                                                                                                                  gatgTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTgcgg               >  1:701293/1‑148 (MQ=255)
                                                                                                                                   atgTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTgcggc              >  2:1963699/1‑148 (MQ=255)
                                                                                                                                   atgTTTTCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTgcggc              >  1:2816896/1‑148 (MQ=255)
                                                                                                                                     gTTTACGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTgcggcgc            >  2:2162901/1‑148 (MQ=255)
                                                                                                                                      ttttCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTgcggcgcg           >  1:2448111/1‑148 (MQ=255)
                                                                                                                                       tttCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTgcggcgcgg          >  1:1577695/1‑148 (MQ=255)
                                                                                                                                       tttCGCGTGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTgcggcgcga          >  2:812799/1‑147 (MQ=255)
                                                                                                                                              tGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGaaaa   <  2:44032/148‑1 (MQ=255)
                                                                                                                                              tGGAGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGaaaa   <  2:2414284/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
AGGCCGGATCATCAAAGGCAATACCAATGACTTCCCGGGTCTTGCGGTTAATTTGGCGCAAACTGGTTAACCCGCGCTGACGCTCTTCAACCACCAGCCAGTCGGTAAACAGCGTAAACCCTTCCAGCATGATGTTTTCGCGTGGCGGAATTAACTCTTCCCACTGTTGCTCATCACGCATACGGGTACGGTATAAGCCAAAGTTTTTGCCGTGGCGGTTGGAACGCAGATAAAAACGATGCTGGTAGTGATCAAGGCTGTATTCGTGATCTTTGCGGCGCGGCAGAAAAA  >  CP009273/1922019‑1922309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑