Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 173,725 G→T 100% G129G (GGC→GGA)  yadS ← UPF0126 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273173,7250GT98.1% 480.0 / 1.0 154G129G (GGC→GGA) yadSUPF0126 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (66/85);  total (68/86)
Fisher's exact test for biased strand distribution p-value = 5.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 164 total aligned reads displayed.

TCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGC  >  CP009273/173580‑173869
                                                                                                                                                 |                                                                                                                                                
tcatACTGGGTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCTATAATACAGGCAGTTGCGTATATTTCTGTACTTAAAATAATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCgcctcc                                                                                                                                                <  1:1604505/148‑1 (MQ=255)
 catACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCa                                                                                                                                               >  2:940555/1‑148 (MQ=255)
    aCTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACg                                                                                                                                            >  2:3532016/1‑148 (MQ=255)
     cTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGc                                                                                                                                           <  2:1033492/148‑1 (MQ=255)
       ggCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCa                                                                                                                                         >  2:2804025/1‑148 (MQ=255)
       ggCTGTTTACAGTGGTACGGAAAATGTGTATTAAGCCGTAGCGTGGAAAATACCGCCGATAATACAGGCAGTTGCGTATATTTATGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCa                                                                                                                                         <  1:1344871/148‑1 (MQ=255)
       ggCTGTTTACAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCa                                                                                                                                         >  2:1593679/1‑148 (MQ=255)
          tGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTa                                                                                                                                      >  2:3466030/1‑148 (MQ=255)
             ttCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAGTGATCCCGCCTCCAACGCCAGTAATg                                                                                                                                   >  1:3031571/1‑148 (MQ=255)
                  gTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGcc                                                                                                                              <  1:1764552/148‑1 (MQ=255)
                   tGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGccc                                                                                                                             >  1:1801759/1‑148 (MQ=255)
                   tGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGccc                                                                                                                             >  2:3678/1‑148 (MQ=255)
                   tGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGccc                                                                                                                             <  1:1019244/148‑1 (MQ=255)
                      tACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATa                                                                                                                          >  2:1362423/1‑148 (MQ=255)
                       aCGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATAc                                                                                                                         >  2:3190897/1‑148 (MQ=255)
                         ggAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACaa                                                                                                                       <  2:3457759/148‑1 (MQ=255)
                           aaaaTGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAAc                                                                                                                     >  1:839694/1‑148 (MQ=255)
                           aaaaTGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAAc                                                                                                                     >  2:611974/1‑148 (MQ=255)
                             aaTGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACACACcg                                                                                                                   >  1:423585/1‑148 (MQ=255)
                             aaTGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACcg                                                                                                                   >  1:2265070/1‑148 (MQ=255)
                                   taataaGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTa                                                                                                             <  1:3532016/148‑1 (MQ=255)
                                      taaGCCGTAGCGTTGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACg                                                                                                          <  2:3017463/148‑1 (MQ=255)
                                        aGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGa                                                                                                        <  1:894673/148‑1 (MQ=255)
                                             tAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGc                                                                                                   >  2:399873/1‑148 (MQ=255)
                                             tAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGc                                                                                                   >  2:2194751/1‑148 (MQ=255)
                                                 gTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTcc                                                                                               <  2:3016927/148‑1 (MQ=255)
                                                  tGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCg                                                                                              >  2:1045453/1‑148 (MQ=255)
                                                   ggACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGc                                                                                             <  2:2924260/148‑1 (MQ=255)
                                                   ggACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGc                                                                                             <  2:3287906/148‑1 (MQ=255)
                                                       aaTACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTa                                                                                         >  2:2471862/1‑148 (MQ=255)
                                                        aTACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTaa                                                                                        >  1:1147684/1‑148 (MQ=255)
                                                         tACCGCCGATAATACCGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTaaa                                                                                       <  1:982762/148‑1 (MQ=255)
                                                         tACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTaaa                                                                                       <  2:2694324/148‑1 (MQ=255)
                                                          aCCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAg                                                                                      <  1:2441453/148‑1 (MQ=255)
                                                           ccgccgATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAgg                                                                                     <  1:1956481/148‑1 (MQ=255)
                                                           ccgccgATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAgg                                                                                     <  2:2868716/148‑1 (MQ=255)
                                                            cgccgATAATACCGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGc                                                                                    <  1:2753447/148‑1 (MQ=255)
                                                             gccgATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCt                                                                                   <  2:839694/148‑1 (MQ=255)
                                                             gccgATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCt                                                                                   <  1:2804025/148‑1 (MQ=255)
                                                               cgATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGTATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCttt                                                                                 <  1:1817906/148‑1 (MQ=255)
                                                               cgATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCttt                                                                                 <  2:2367332/148‑1 (MQ=255)
                                                               cgATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCttt                                                                                 <  2:774078/148‑1 (MQ=255)
                                                                 ataataCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTAt                                                                               <  1:428466/148‑1 (MQ=255)
                                                                 ataataCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTAt                                                                               <  1:518943/148‑1 (MQ=255)
                                                                 ataataCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTAt                                                                               <  2:2645385/148‑1 (MQ=255)
                                                                  taataCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTAtt                                                                              <  1:2940358/148‑1 (MQ=255)
                                                                    ataCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCa                                                                            <  2:1951056/148‑1 (MQ=255)
                                                                    ataCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCa                                                                            >  1:784292/1‑148 (MQ=255)
                                                                    ataCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCa                                                                            <  2:2925739/148‑1 (MQ=255)
                                                                     taCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCAc                                                                           <  1:949875/148‑1 (MQ=255)
                                                                      aCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACg                                                                          >  2:633739/1‑148 (MQ=255)
                                                                          gCATTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCaa                                                                      <  1:37709/148‑1 (MQ=255)
                                                                          gCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCaa                                                                      <  1:37704/148‑1 (MQ=255)
                                                                           cAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAAt                                                                     >  1:163619/1‑148 (MQ=255)
                                                                               tGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCg                                                                 <  1:2150998/148‑1 (MQ=255)
                                                                               tGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGACCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCg                                                                 <  2:1463749/148‑1 (MQ=255)
                                                                                    aGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACaaa                                                            <  2:2871620/148‑1 (MQ=255)
                                                                                     gATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAac                                                           <  1:47882/148‑1 (MQ=255)
                                                                                      aTTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAaca                                                          >  2:1301692/1‑148 (MQ=255)
                                                                                      aTTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAaca                                                          >  2:1301683/1‑148 (MQ=255)
                                                                                      aTTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAaca                                                          <  1:3124745/148‑1 (MQ=255)
                                                                                           tGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGcc                                                     <  1:1329458/148‑1 (MQ=255)
                                                                                                 tAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCa                                               <  2:1993313/148‑1 (MQ=255)
                                                                                                  aaaaTCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGGTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCaa                                              <  1:1050293/148‑1 (MQ=255)
                                                                                                  aaaaTCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCaa                                              <  1:2409322/148‑1 (MQ=255)
                                                                                                  aaaaTCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCaa                                              <  2:2435328/148‑1 (MQ=255)
                                                                                                   aaaTCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAAc                                             >  1:1196252/1‑148 (MQ=255)
                                                                                                    aaTCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACg                                            >  1:3489554/1‑148 (MQ=255)
                                                                                                     aTCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACgg                                           >  2:1325815/1‑148 (MQ=255)
                                                                                                      tCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGc                                          >  1:3466109/1‑148 (MQ=255)
                                                                                                       cATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCg                                         >  1:3297739/1‑148 (MQ=255)
                                                                                                        aTGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGt                                        <  1:1523509/148‑1 (MQ=255)
                                                                                                         tGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTc                                       <  1:1485760/148‑1 (MQ=255)
                                                                                                         tGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTc                                       <  2:799492/148‑1 (MQ=255)
                                                                                                           gggATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCa                                     >  1:2698840/1‑148 (MQ=255)
                                                                                                            ggATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAg                                    <  1:2388307/148‑1 (MQ=255)
                                                                                                              aTTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCa                                  <  2:1790711/148‑1 (MQ=255)
                                                                                                                      ggCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAAc                          <  2:521044/148‑1 (MQ=255)
                                                                                                                      ggCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGAGTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAAc                          <  2:520617/148‑1 (MQ=255)
                                                                                                                         cAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATc                       >  2:643083/1‑148 (MQ=255)
                                                                                                                          aGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATcc                      >  2:2691203/1‑148 (MQ=255)
                                                                                                                          aGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATcc                      >  1:1837219/1‑148 (MQ=255)
                                                                                                                           gAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCa                     <  1:611974/148‑1 (MQ=255)
                                                                                                                            aaCATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCAt                    >  1:3169239/1‑148 (MQ=255)
                                                                                                                              cATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCAttt                  <  1:2963955/148‑1 (MQ=255)
                                                                                                                                tCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCACCATCCATTTTg                >  2:2532700/1‑148 (MQ=255)
                                                                                                                                tCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTg                >  2:2975959/1‑148 (MQ=255)
                                                                                                                                tCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTg                >  2:2098888/1‑148 (MQ=255)
                                                                                                                                 cACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTgg               <  2:2352156/148‑1 (MQ=255)
                                                                                                                                  aCGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGt              <  1:942347/148‑1 (MQ=255)
                                                                                                                                   cGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTa             <  2:1862794/148‑1 (MQ=255)
                                                                                                                                        gATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGt        >  2:1788704/1‑148 (MQ=255)
                                                                                                                                          tCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCt      >  2:2084668/1‑148 (MQ=255)
                                                                                                                                           cccGACTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCtt     <  2:3289354/148‑1 (MQ=255)
                                                                                                                                            ccgcctCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTtg    >  1:3020996/1‑148 (MQ=255)
                                                                                                                                            ccgcctCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTtg    >  1:3021108/1‑148 (MQ=255)
                                                                                                                                             cgcctcCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTtgg   >  1:1756301/1‑148 (MQ=255)
                                                                                                                                             cgcctcCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTtgg   >  2:3461357/1‑148 (MQ=255)
                                                                                                                                             cgcctcCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTtgg   >  1:1263484/1‑148 (MQ=255)
                                                                                                                                             cgcctcCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTtgg   >  1:411630/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGC  >  CP009273/173580‑173869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑