Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,041,721 C→A 12.0% I1101I (ATC→ATA)  yeeJ → putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,041,7210CA12.0% 714.2 / 42.5 215I1101I (ATC→ATA) yeeJputative adhesin
Reads supporting (aligned to +/- strand):  ref base C (100/88);  new base A (12/15);  total (112/103)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 216 total aligned reads displayed.

ATCATTGGGAATGGCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTG  >  CP009273/2041575‑2041868
                                                                                                                                                  |                                                                                                                                                   
aTCATTGGGAATGGCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCa                                                                                                                                                    >  2:2368849/1‑148 (MQ=255)
 tCATTGGGAATGGCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCAc                                                                                                                                                   <  2:248979/148‑1 (MQ=255)
  cATTGGGAATGGCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACt                                                                                                                                                  <  2:1565716/148‑1 (MQ=255)
  cATTGGGAATGGCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACt                                                                                                                                                  <  1:2818681/148‑1 (MQ=255)
          aTGGCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGc                                                                                                                                             <  2:1962344/145‑1 (MQ=255)
                tGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGa                                                                                                                                    >  1:2389859/1‑148 (MQ=255)
                  gATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAg                                                                                                                                  <  2:931590/148‑1 (MQ=255)
                   aTGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAgc                                                                                                                                 >  1:3105145/1‑148 (MQ=255)
                    tGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTAGAAAATAACGGTATTGCCATCACTCAGGACAATGGGGAAgcg                                                                                                                                >  2:949516/1‑148 (MQ=255)
                         cAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGt                                                                                                                           <  1:2579638/148‑1 (MQ=255)
                         cAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAAAAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGt                                                                                                                           >  2:2133458/1‑148 (MQ=255)
                           aCTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCa                                                                                                                         <  1:1214408/148‑1 (MQ=255)
                            ctctGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGTCAATGGGGAAGCGCATGTCAc                                                                                                                        >  1:457341/1‑148 (MQ=255)
                              ctGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGc                                                                                                                      >  2:3535965/1‑148 (MQ=255)
                                gACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTg                                                                                                                    >  2:3125945/1‑148 (MQ=255)
                                 aCAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGa                                                                                                                   >  2:3257189/1‑148 (MQ=255)
                                    gCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAg                                                                                                                >  2:2277576/1‑148 (MQ=255)
                                    gCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAg                                                                                                                <  2:2749531/148‑1 (MQ=255)
                                     cAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCGGAAAgg                                                                                                               >  2:3567040/1‑148 (MQ=255)
                                       aCAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTa                                                                                                             >  2:1351824/1‑148 (MQ=255)
                                       aCAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTa                                                                                                             <  1:543751/148‑1 (MQ=255)
                                         aGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTaaa                                                                                                           >  2:217972/1‑148 (MQ=255)
                                           tGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTaaaaa                                                                                                         >  1:2670570/1‑148 (MQ=255)
                                            gAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTaaaaaa                                                                                                        >  2:1005754/1‑148 (MQ=255)
                                              aaGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGc                                                                                                      <  2:2064430/148‑1 (MQ=255)
                                               aGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAAGGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCg                                                                                                     >  2:2998074/1‑148 (MQ=255)
                                                 aTCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCACTGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCggg                                                                                                   >  2:2251246/1‑148 (MQ=255)
                                                   ccGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGCCa                                                                                                 <  2:3350348/148‑1 (MQ=255)
                                                     gTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACg                                                                                               >  2:2636870/1‑148 (MQ=255)
                                                        gAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCAt                                                                                            <  1:1883014/148‑1 (MQ=255)
                                                          atcatcCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATAc                                                                                          >  1:3006583/1‑148 (MQ=255)
                                                          atcatcCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATAc                                                                                          <  2:715616/148‑1 (MQ=255)
                                                           tcatcCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACg                                                                                         <  2:1612942/148‑1 (MQ=255)
                                                           tcatcCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACg                                                                                         >  2:3033911/1‑148 (MQ=255)
                                                           tcatcCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACg                                                                                         >  2:1257348/1‑148 (MQ=255)
                                                           tcatcCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACg                                                                                         >  1:136731/1‑148 (MQ=255)
                                                             atcCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGt                                                                                       >  2:662488/1‑148 (MQ=255)
                                                             atcCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGt                                                                                       <  2:1767168/148‑1 (MQ=255)
                                                                    ggCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCa                                                                                >  1:803510/1‑148 (MQ=255)
                                                                        gggATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGc                                                                            >  2:426901/1‑148 (MQ=255)
                                                                         ggATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCt                                                                           >  2:772117/1‑148 (MQ=255)
                                                                          gATAACGGTAAACTTGACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTg                                                                          <  1:3122942/148‑1 (MQ=255)
                                                                           aTAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTgg                                                                         >  1:879889/1‑148 (MQ=255)
                                                                            tAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTggg                                                                        >  1:3237404/1‑148 (MQ=255)
                                                                            tAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTggg                                                                        <  1:2263950/148‑1 (MQ=255)
                                                                             aaCGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGt                                                                       <  2:1149631/148‑1 (MQ=255)
                                                                              aCGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGta                                                                      >  1:2663274/1‑148 (MQ=255)
                                                                               cGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGtaa                                                                     <  2:1866879/148‑1 (MQ=255)
                                                                               cGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGtaa                                                                     <  2:1548201/148‑1 (MQ=255)
                                                                                   aaaCTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAAc                                                                 <  2:316423/148‑1 (MQ=255)
                                                                                   aaaCGTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAAc                                                                 <  2:2111585/148‑1 (MQ=255)
                                                                                    aaCTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACa                                                                <  1:2768128/148‑1 (MQ=255)
                                                                                    aaCTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACa                                                                <  1:3163838/148‑1 (MQ=255)
                                                                                     aCTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACaa                                                               >  1:763069/1‑148 (MQ=255)
                                                                                     aCTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACaa                                                               <  2:2699773/148‑1 (MQ=255)
                                                                                     aCTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACaa                                                               <  1:1349527/148‑1 (MQ=255)
                                                                                      cTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAAt                                                              <  2:258568/148‑1 (MQ=255)
                                                                                        tCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATAc                                                            <  1:3290302/148‑1 (MQ=255)
                                                                                        tCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATAc                                                            <  1:3290089/148‑1 (MQ=255)
                                                                                          aCCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCa                                                          <  1:2340435/148‑1 (MQ=255)
                                                                                           ccATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAg                                                         >  1:746940/1‑148 (MQ=255)
                                                                                            cATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGt                                                        <  2:14644/148‑1 (MQ=255)
                                                                                              tGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGa                                                      >  1:3279056/1‑148 (MQ=255)
                                                                                              tGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGa                                                      <  1:3255404/148‑1 (MQ=255)
                                                                                              tGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGa                                                      <  1:3125945/148‑1 (MQ=255)
                                                                                               gCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGAt                                                     >  2:18929/1‑148 (MQ=255)
                                                                                               gCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGAt                                                     >  1:3145233/1‑148 (MQ=255)
                                                                                                ccACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGAtt                                                    <  1:2347466/148‑1 (MQ=255)
                                                                                                 cacaGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTc                                                   <  1:126278/148‑1 (MQ=255)
                                                                                                      gACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGc                                              >  1:2469411/1‑148 (MQ=255)
                                                                                                           tGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTa                                         <  1:3293497/148‑1 (MQ=255)
                                                                                                               gCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACAt                                     >  2:1156233/1‑148 (MQ=255)
                                                                                                                      ttACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGc                              >  1:3438981/1‑148 (MQ=255)
                                                                                                                      ttACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGc                              >  2:2876482/1‑148 (MQ=255)
                                                                                                                       tACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCg                             >  2:2925397/1‑148 (MQ=255)
                                                                                                                         cccTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGa                           >  1:102120/1‑148 (MQ=255)
                                                                                                                           cTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACa                         >  1:197645/1‑148 (MQ=255)
                                                                                                                            ttGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACaa                        >  1:1737912/1‑148 (MQ=255)
                                                                                                                            ttGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACaa                        >  2:1137961/1‑148 (MQ=255)
                                                                                                                             tGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACaaa                       >  1:2760417/1‑148 (MQ=255)
                                                                                                                             tGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACaaa                       >  2:2494923/1‑148 (MQ=255)
                                                                                                                                aaaTAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGcc                    >  2:2006690/1‑148 (MQ=255)
                                                                                                                                 aaTAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCt                   <  1:3400481/148‑1 (MQ=255)
                                                                                                                                  aTAACGGTATTGCCATCACTCAGGCCCAGGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGGGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGAGACAGCCTc                  >  2:1505278/1‑148 (MQ=255)
                                                                                                                                   tAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCg                 >  1:539265/1‑148 (MQ=255)
                                                                                                                                      cGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCt              >  1:201778/1‑148 (MQ=255)
                                                                                                                                       ggTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTc             <  1:1792890/148‑1 (MQ=255)
                                                                                                                                       ggTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTc             <  1:2117541/148‑1 (MQ=255)
                                                                                                                                         tATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAg           >  1:491199/1‑148 (MQ=255)
                                                                                                                                          aTTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAgg          >  2:3457985/1‑148 (MQ=255)
                                                                                                                                           ttGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGt         <  1:3535965/148‑1 (MQ=255)
                                                                                                                                            tGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGtt        >  1:1674382/1‑148 (MQ=255)
                                                                                                                                            tGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGtt        >  1:3060124/1‑148 (MQ=255)
                                                                                                                                             gCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTg       <  1:3554840/148‑1 (MQ=255)
                                                                                                                                              ccATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGt      >  1:1481968/1‑148 (MQ=255)
                                                                                                                                              ccATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGt      >  1:1295471/1‑148 (MQ=255)
                                                                                                                                               cATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTc     >  2:2085997/1‑148 (MQ=255)
                                                                                                                                               cATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTc     <  2:752483/148‑1 (MQ=255)
                                                                                                                                                 tCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCt   >  2:2517148/1‑148 (MQ=255)
                                                                                                                                                  cACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTg  >  2:1703178/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
ATCATTGGGAATGGCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTG  >  CP009273/2041575‑2041868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑