Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,049,111 C→A 100% T190T (ACC→ACA)  amn → AMP nucleosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,049,1110CA97.0% 696.2 / 11.7 234T190T (ACC→ACA) amnAMP nucleosidase
Reads supporting (aligned to +/- strand):  ref base C (4/3);  new base A (108/119);  total (112/122)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 234 total aligned reads displayed.

TAACTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAACTGG  >  CP009273/2048966‑2049255
                                                                                                                                                 |                                                                                                                                                
tAACTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAgg                                                                                                                                                <  1:269633/148‑1 (MQ=255)
  aCTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTa                                                                                                                                              >  2:1553363/1‑148 (MQ=255)
  aCTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTa                                                                                                                                              >  2:1712797/1‑148 (MQ=255)
   cTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTAc                                                                                                                                             >  1:1036562/1‑148 (MQ=255)
     cGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGc                                                                                                                                           <  2:1790204/148‑1 (MQ=255)
      gCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGcc                                                                                                                                          >  1:1453882/1‑148 (MQ=255)
       cTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCa                                                                                                                                         <  1:489155/148‑1 (MQ=255)
       cTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCa                                                                                                                                         <  2:3075951/148‑1 (MQ=255)
         aCTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGt                                                                                                                                       >  1:3487095/1‑148 (MQ=255)
         aCTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGt                                                                                                                                       >  1:2031924/1‑148 (MQ=255)
         aCTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGt                                                                                                                                       >  1:1481362/1‑148 (MQ=255)
         aCTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGt                                                                                                                                       >  1:1151643/1‑148 (MQ=255)
               cGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACa                                                                                                                                 <  1:1365150/148‑1 (MQ=255)
                gACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACAt                                                                                                                                >  2:3133006/1‑148 (MQ=255)
                         aCTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCg                                                                                                                       >  1:2412044/1‑148 (MQ=255)
                            ggCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTc                                                                                                                    >  1:3196154/1‑148 (MQ=255)
                             gcgcAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCg                                                                                                                   >  2:2182355/1‑148 (MQ=255)
                                   aTTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGt                                                                                                             <  2:3240486/148‑1 (MQ=255)
                                     tGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGttt                                                                                                           <  2:1481362/148‑1 (MQ=255)
                                       gCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTAc                                                                                                         <  2:3400591/148‑1 (MQ=255)
                                         gATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCa                                                                                                       <  2:2265635/148‑1 (MQ=255)
                                         gATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCa                                                                                                       <  2:875546/148‑1 (MQ=255)
                                            gAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACt                                                                                                    <  2:2482953/148‑1 (MQ=255)
                                             aaaCTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTa                                                                                                   <  2:728050/148‑1 (MQ=255)
                                             aaaCTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTa                                                                                                   <  1:1770611/148‑1 (MQ=255)
                                               aCTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGACTTGTTTACCAACTaca                                                                                                 >  2:2964984/1‑148 (MQ=255)
                                                cTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTacac                                                                                                >  1:3190602/1‑148 (MQ=255)
                                                cTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTacac                                                                                                <  2:3408911/148‑1 (MQ=255)
                                                cTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTacac                                                                                                <  2:2377016/148‑1 (MQ=255)
                                                  gCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTacacac                                                                                              >  2:1965316/1‑148 (MQ=255)
                                                    aGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGt                                                                                            <  1:307991/148‑1 (MQ=255)
                                                      aCGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTa                                                                                          >  1:3243637/1‑148 (MQ=255)
                                                      aCGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTa                                                                                          <  2:2153456/148‑1 (MQ=255)
                                                      aCGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTa                                                                                          <  2:746754/148‑1 (MQ=255)
                                                      aCGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTa                                                                                          <  2:456306/148‑1 (MQ=255)
                                                      aCGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTa                                                                                          >  1:2843249/1‑148 (MQ=255)
                                                        ggCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTAtg                                                                                        >  2:3010584/1‑148 (MQ=255)
                                                        ggCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTAtg                                                                                        <  1:880494/148‑1 (MQ=255)
                                                          cATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTAtgtg                                                                                      >  1:919539/1‑148 (MQ=255)
                                                            tttATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGa                                                                                    >  2:1534027/1‑148 (MQ=255)
                                                             ttATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGAt                                                                                   >  2:3111051/1‑148 (MQ=255)
                                                                tcatcCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGaa                                                                                <  1:3073651/148‑1 (MQ=255)
                                                                tcatcCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGGTATGTGGATGaa                                                                                >  1:3302078/1‑148 (MQ=255)
                                                                 catcCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAt                                                                               >  2:43733/1‑148 (MQ=255)
                                                                 catcCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCAATATACAGGTACGCCAGTTGAACATTTTCAGCCCGTCGTCTTGTTTACCAACTACACACGTTATGTTGATGAAt                                                                               >  1:2938362/1‑148 (MQ=255)
                                                                     cAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAttcgt                                                                           <  1:1534027/148‑1 (MQ=255)
                                                                     cAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAttcgt                                                                           <  1:2274987/148‑1 (MQ=255)
                                                                        cTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAt                                                                               >  1:807482/1‑141 (MQ=255)
                                                                         tGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATtcgttcgt                                                                       <  2:2436376/148‑1 (MQ=255)
                                                                         tGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATtcgttcgt                                                                       <  2:20055/148‑1 (MQ=255)
                                                                          gAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTcgttcgtt                                                                      <  1:2964925/148‑1 (MQ=255)
                                                                           aaTTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTg                                                                     <  2:1188236/148‑1 (MQ=255)
                                                                           aaTTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTg                                                                     <  2:3428873/148‑1 (MQ=255)
                                                                              tctcCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTgggg                                                                  <  2:3103845/148‑1 (MQ=255)
                                                                              tctcCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTgggg                                                                  >  2:2770495/1‑148 (MQ=255)
                                                                               ctcCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGa                                                                 <  1:3133006/148‑1 (MQ=255)
                                                                               ctcCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGa                                                                 <  2:2464031/148‑1 (MQ=255)
                                                                                    cGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAg                                                            <  2:1035602/148‑1 (MQ=255)
                                                                                    cGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAg                                                            >  2:2541193/1‑148 (MQ=255)
                                                                                    cGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCAAGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTGTTTTTTACCAACAACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAg                                                            >  1:1692687/1‑148 (MQ=255)
                                                                                     gCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGc                                                           <  2:3487095/148‑1 (MQ=255)
                                                                                      cTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGcc                                                          >  2:3259936/1‑148 (MQ=255)
                                                                                        aTCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAg                                                        <  2:2333740/148‑1 (MQ=255)
                                                                                         tCGCATTTTGATGCGCGCCGCGTCGATTTTTACCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACAATTTCAGCCGTTCGTCTTGTTTACCAACTAAACACGGTATGTGGATGAATTCGTTCGTTGGGGATTCAGCCAGa                                                       >  1:2185764/1‑148 (MQ=255)
                                                                                         tCGCATTTTGATGCGCGCCGCGTCGATTTTTACCTCGCACGGTTGCGCCAATATACAGGTACGCCAGTTGAACAATTTCAGCCCTTAGTCTTGTTTACCAACTACACACGATATGTGGATGAATTAGTTCGTTGGGGATGCAGCCAGa                                                       >  1:1129255/1‑148 (MQ=255)
                                                                                          cGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGAt                                                      >  2:2616079/1‑148 (MQ=255)
                                                                                           gCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATc                                                     <  1:317575/148‑1 (MQ=255)
                                                                                           gCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATc                                                     <  2:3366200/148‑1 (MQ=255)
                                                                                            cATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATcc                                                    >  2:987940/1‑148 (MQ=255)
                                                                                            cATTTTGATGCGCGCCGCGTCGATTTGTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATcc                                                    <  2:2916125/148‑1 (MQ=255)
                                                                                                ttGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGTAGCCAGATCCTCGa                                                <  1:198511/148‑1 (MQ=255)
                                                                                                   aTGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATcc                                             <  1:3477378/148‑1 (MQ=255)
                                                                                                     gcgcgcCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTg                                           <  2:3145664/148‑1 (MQ=255)
                                                                                                      cgcgcCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGGTCGTTGGGGATGCAGCCCGATCCTCGATCCGGa                                          >  2:2140009/1‑148 (MQ=255)
                                                                                                        cgccgcGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATa                                        <  2:1380680/148‑1 (MQ=255)
                                                                                                           cgcgTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTc                                     <  2:1592000/148‑1 (MQ=255)
                                                                                                            gcgTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTcc                                    <  1:2474859/148‑1 (MQ=255)
                                                                                                            gcgTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTcc                                    <  2:1994005/148‑1 (MQ=255)
                                                                                                             cgTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTccc                                   >  2:268794/1‑148 (MQ=255)
                                                                                                             cgTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTccc                                   >  2:269935/1‑148 (MQ=255)
                                                                                                              gTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCt                                  >  2:1336721/1‑148 (MQ=255)
                                                                                                                cGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTAc                                <  1:56899/148‑1 (MQ=255)
                                                                                                                 gATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACa                               >  2:1885596/1‑148 (MQ=255)
                                                                                                                  aTTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACAt                              >  2:296632/1‑148 (MQ=255)
                                                                                                                   tttttCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACAtt                             >  1:328795/1‑148 (MQ=255)
                                                                                                                    ttttCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTg                            <  1:235791/148‑1 (MQ=255)
                                                                                                                       tCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGccc                         <  2:1190748/148‑1 (MQ=255)
                                                                                                                               aCGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTtgt                 >  1:2860164/1‑148 (MQ=255)
                                                                                                                                cGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTtgtg                <  2:1107445/148‑1 (MQ=255)
                                                                                                                                   ttGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTg             >  2:1128508/1‑148 (MQ=255)
                                                                                                                                    tGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTgg            >  2:565123/1‑148 (MQ=255)
                                                                                                                                          aTTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGaa      >  1:372102/1‑148 (MQ=255)
                                                                                                                                          aTTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGaa      >  1:72723/1‑148 (MQ=255)
                                                                                                                                           ttATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAAc     >  1:565371/1‑148 (MQ=255)
                                                                                                                                           ttATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAAc     <  1:924497/148‑1 (MQ=255)
                                                                                                                                            tataCAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAACt    >  2:2136459/1‑148 (MQ=255)
                                                                                                                                            tataCAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAACt    >  1:728246/1‑148 (MQ=255)
                                                                                                                                             ataCAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAACTg   >  1:2930726/1‑148 (MQ=255)
                                                                                                                                             ataCAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAACTg   >  1:1599260/1‑148 (MQ=255)
                                                                                                                                              taCAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAACTgg  >  1:2386168/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TAACTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAACTGG  >  CP009273/2048966‑2049255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑