Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 185,393 A→T 100% F201I (TTC→ATC)  map ← methionine aminopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273185,3930AT96.6% 644.1 / 9.6 205F201I (TTC→ATC) mapmethionine aminopeptidase
Reads supporting (aligned to +/- strand):  ref base A (1/6);  new base T (114/83);  total (116/89)
Fisher's exact test for biased strand distribution p-value = 4.47e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 206 total aligned reads displayed.

GTCAGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCGC  >  CP009273/185248‑185540
                                                                                                                                                 |                                                                                                                                                   
gTCAGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCg                                                                                                                                                   <  2:1064967/148‑1 (MQ=255)
gTCAGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACg                                                                                                                                                   <  2:2597620/148‑1 (MQ=255)
 tCAGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGt                                                                                                                                                  >  1:343686/1‑148 (MQ=255)
   aGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCa                                                                                                                                                <  1:1706144/148‑1 (MQ=255)
    gAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCAt                                                                                                                                               >  1:2264067/1‑148 (MQ=255)
     aaTTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATc                                                                                                                                              >  1:2273599/1‑148 (MQ=255)
      aTTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATcc                                                                                                                                             >  1:1000594/1‑148 (MQ=255)
       tttCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATccc                                                                                                                                            <  1:2683692/148‑1 (MQ=255)
          cGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAgg                                                                                                                                         <  2:2525115/148‑1 (MQ=255)
          cGCAGCCGTTATCAGTCACCACAAGAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAgg                                                                                                                                         <  2:3235252/148‑1 (MQ=255)
           gCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGt                                                                                                                                        <  1:3305436/148‑1 (MQ=255)
             aGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGttt                                                                                                                                      <  1:2767562/148‑1 (MQ=255)
              gCCGTTTTCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTc                                                                                                                                     <  2:894065/148‑1 (MQ=255)
               ccGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCa                                                                                                                                    <  2:583449/148‑1 (MQ=255)
                cGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAg                                                                                                                                   <  1:2339350/148‑1 (MQ=255)
                  ttATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAGCGTCATCCCAGGTTTCAGTa                                                                                                                                 >  2:1851367/1‑148 (MQ=255)
                     tCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTGACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTacga                                                                                                                              <  2:715329/148‑1 (MQ=255)
                      cAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTacgac                                                                                                                             >  2:2217540/1‑148 (MQ=255)
                       aGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTacgacg                                                                                                                            <  2:3335618/148‑1 (MQ=255)
                        gTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGt                                                                                                                           >  1:3109324/1‑148 (MQ=255)
                         tCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGtt                                                                                                                          <  2:2410857/148‑1 (MQ=255)
                         tCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGtt                                                                                                                          >  1:2124815/1‑148 (MQ=255)
                         tCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGtt                                                                                                                          <  2:1048606/148‑1 (MQ=255)
                         tCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGtt                                                                                                                          >  2:3135105/1‑148 (MQ=255)
                            ccacAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGt                                                                                                                       <  2:1649622/148‑1 (MQ=255)
                            ccacAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGt                                                                                                                       >  1:2708101/1‑148 (MQ=255)
                            ccacAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGt                                                                                                                       <  1:2729329/148‑1 (MQ=255)
                            ccacAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGt                                                                                                                       <  1:947920/148‑1 (MQ=255)
                             cacaATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGtt                                                                                                                      >  1:2242298/1‑148 (MQ=255)
                               caATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTc                                                                                                                    <  1:1305350/148‑1 (MQ=255)
                                aaTAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCa                                                                                                                   >  2:499176/1‑148 (MQ=255)
                                 aTAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCAc                                                                                                                  >  2:1313140/1‑148 (MQ=255)
                                  tAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTTAGTACGACGTTGGTTTCACg                                                                                                                 >  1:257767/1‑148 (MQ=255)
                                     tATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGa                                                                                                              >  2:2071509/1‑148 (MQ=255)
                                     tATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGa                                                                                                              >  2:272798/1‑148 (MQ=255)
                                      aTGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAg                                                                                                             <  2:788902/148‑1 (MQ=255)
                                       tGCTCATATTGTGCTGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGt                                                                                                            <  1:2033829/148‑1 (MQ=255)
                                        gCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTc                                                                                                           >  1:474124/1‑148 (MQ=255)
                                             tatTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGt                                                                                                      >  1:796640/1‑148 (MQ=255)
                                               ttGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTgc                                                                                                    >  2:2984466/1‑148 (MQ=255)
                                               ttGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTgc                                                                                                    >  2:2736190/1‑148 (MQ=255)
                                                tgtgCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTgca                                                                                                   >  1:1496201/1‑148 (MQ=255)
                                                 gtgCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTgcag                                                                                                  <  1:179654/148‑1 (MQ=255)
                                                 gtgCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTgcag                                                                                                  <  1:1413516/148‑1 (MQ=255)
                                                   gCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTgcagca                                                                                                >  1:429678/1‑148 (MQ=255)
                                                   gCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTgcagca                                                                                                >  1:1467375/1‑148 (MQ=255)
                                                     aGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCAcc                                                                                              >  2:1949014/1‑148 (MQ=255)
                                                           gCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGt                                                                                        >  1:770524/1‑148 (MQ=255)
                                                             tGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGttc                                                                                      >  1:1661674/1‑148 (MQ=255)
                                                              gCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGttct                                                                                     <  2:1375283/148‑1 (MQ=255)
                                                              gCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGttct                                                                                     <  1:3139031/148‑1 (MQ=255)
                                                               cGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTacgacg                                                                                                                            >  2:1186394/1‑108 (MQ=255)
                                                               cGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTacgacg                                                                                                                            <  1:1186394/108‑1 (MQ=255)
                                                               cGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGttctt                                                                                    >  1:2231810/1‑148 (MQ=255)
                                                                taTCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTTGCTCGATGGTGATCGTCATCCCAGTTTTCAGTACGACGTTGGTTTCACGGGAGTAATAGTGCATCACCTGCGGttcttc                                                                                   <  2:713182/147‑1 (MQ=255)
                                                                gATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCGTGTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGttcttc                                                                                   <  1:2387523/148‑1 (MQ=255)
                                                                  tCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCAt                                                                                 >  2:2591616/1‑148 (MQ=255)
                                                                  tCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCAt                                                                                 >  2:663850/1‑148 (MQ=255)
                                                                   cTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATg                                                                                >  1:1589792/1‑148 (MQ=255)
                                                                    tttGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATgg                                                                               <  1:252512/148‑1 (MQ=255)
                                                                      tGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGaa                                                                             <  1:1010026/148‑1 (MQ=255)
                                                                       ggTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAg                                                                            <  2:1897474/148‑1 (MQ=255)
                                                                            ttACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCcgcg                                                                       <  1:44693/148‑1 (MQ=255)
                                                                                  tCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAAt                                                                 >  2:1304156/1‑148 (MQ=255)
                                                                                  tCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAAt                                                                 >  1:244698/1‑148 (MQ=255)
                                                                                   ccAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATa                                                                <  1:383090/148‑1 (MQ=255)
                                                                                   ccAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATa                                                                >  2:2839383/1‑148 (MQ=255)
                                                                                   ccAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATa                                                                <  1:756553/148‑1 (MQ=255)
                                                                                         aTCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCgtgt                                                          <  2:2318548/148‑1 (MQ=255)
                                                                                           cTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTcc                                                        >  1:1312973/1‑148 (MQ=255)
                                                                                           cTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTcc                                                        >  1:1125960/1‑148 (MQ=255)
                                                                                              tCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCa                                                     >  2:1569451/1‑148 (MQ=255)
                                                                                                 tGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAata                                                  >  1:1567937/1‑148 (MQ=255)
                                                                                                  ggTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAatat                                                 <  2:382473/148‑1 (MQ=255)
                                                                                                    tGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTc                                               <  2:1000730/148‑1 (MQ=255)
                                                                                                     gCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCa                                              >  2:3088224/1‑148 (MQ=255)
                                                                                                         aTCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacgaa                                          >  1:833309/1‑148 (MQ=255)
                                                                                                         aTCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacgaa                                          >  1:2534138/1‑148 (MQ=255)
                                                                                                           ctctTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacgaacg                                        <  1:1913352/148‑1 (MQ=255)
                                                                                                            tctTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacgaacga                                       >  2:1047642/1‑148 (MQ=255)
                                                                                                                ttttACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGga                                   >  1:221544/1‑148 (MQ=255)
                                                                                                                  ttACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGgaga                                 >  2:267407/1‑148 (MQ=255)
                                                                                                                     cccGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGc                              >  2:3361600/1‑148 (MQ=255)
                                                                                                                      ccGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCTCCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGcc                             <  2:2273599/148‑1 (MQ=255)
                                                                                                                      ccGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGcc                             <  1:2837115/148‑1 (MQ=255)
                                                                                                                       cgcgTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCt                            >  2:1235569/1‑148 (MQ=255)
                                                                                                                           ttGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTg                        >  2:2396760/1‑148 (MQ=255)
                                                                                                                            tGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGc                       <  1:2984573/148‑1 (MQ=255)
                                                                                                                                  ttGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGAc                 >  2:132730/1‑148 (MQ=255)
                                                                                                                                   tGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACa                >  1:2758391/1‑148 (MQ=255)
                                                                                                                                   tGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACa                >  1:2758338/1‑148 (MQ=255)
                                                                                                                                    ggCTCGATGGTGATCGTCATCCCAGGGTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACaa               <  2:3171562/148‑1 (MQ=255)
                                                                                                                                     gCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACaaa              <  2:1762069/148‑1 (MQ=255)
                                                                                                                                      cTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAAt             >  1:2934566/1‑148 (MQ=255)
                                                                                                                                           tGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTg        >  2:212076/1‑148 (MQ=255)
                                                                                                                                           tGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTg        >  2:2087004/1‑148 (MQ=255)
                                                                                                                                            ggTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGa       <  2:770524/148‑1 (MQ=255)
                                                                                                                                             gTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGaa      <  2:482486/148‑1 (MQ=255)
                                                                                                                                             gTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGaa      >  1:705018/1‑148 (MQ=255)
                                                                                                                                                 aCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATcgc  >  2:3482381/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GTCAGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCGC  >  CP009273/185248‑185540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑