BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,082,332	T→A	5.2%	Q93L (CAA→CTA)	yeeZ ←	putative epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,082,332	0	T	A	5.2%	473.1 / 13.2	154	Q93L (CAA→CTA)	yeeZ	putative epimerase
Reads supporting (aligned to +/- strand): ref base T (81/65); new base A (3/5); total (84/70)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

Only 100 of 156 total aligned reads displayed.

GTTCTTCTAATACTCGTCCACTGTTGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGC  >  CP009273/2082186‑2082479

gTTCTTCTAATACTCGTCCACTGTTGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTg                                                                                                                                                    <  2:2738920/148‑1 (MQ=255)

 ttcttcTAATACTCGTCCACTGTTGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGt                                                                                                                                                   >  1:3452878/1‑148 (MQ=255)

     tctAATACTCGTCCACTGTTTGTTACTGGATTTCGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATTGACAGATGTTGAGCTGGTTAAAATAATGCGGGGAATACGATGGGCCAGCGCGCGATCCACTAACTCTTGTACCg                                                                                                                                               <  2:3048227/148‑1 (MQ=255)

       tAATACTCGTCCACTGTTGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCt                                                                                                                                             <  2:500009/148‑1 (MQ=255)

       tAATACTCGTCCACTGTTGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCt                                                                                                                                             <  2:2292691/148‑1 (MQ=255)

          tACTCGTCCACTGTTGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGt                                                                                                                                          >  2:3019684/1‑148 (MQ=255)

           aCTCGTCCACTGTTGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTa                                                                                                                                         >  2:641954/1‑148 (MQ=255)

               gTCCACTGTTGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATa                                                                                                                                     >  1:1453887/1‑148 (MQ=255)

                tCCACTGTTGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAg                                                                                                                                    >  1:294565/1‑148 (MQ=255)

                tCCACTGTTGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAg                                                                                                                                    >  1:294777/1‑148 (MQ=255)

                tCCACTGTTGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAg                                                                                                                                    >  1:2044306/1‑148 (MQ=255)

                 ccACTGTTGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGa                                                                                                                                   <  2:715334/148‑1 (MQ=255)

                 ccACTGTTGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGa                                                                                                                                   <  2:715236/148‑1 (MQ=255)

                 ccACTGGTGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGa                                                                                                                                   <  2:2091163/148‑1 (MQ=255)

                  cACTGTTGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGaa                                                                                                                                  <  1:819096/148‑1 (MQ=255)

                   aCTGTTGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTAGTACCGCTTGTAAATAGAAc                                                                                                                                 <  1:2764632/148‑1 (MQ=255)

                   aCTGTTGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTAGTACCGCTTGTAAATAGAAc                                                                                                                                 <  1:2821500/148‑1 (MQ=255)

                     tGTTGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTc                                                                                                                               <  1:1203716/148‑1 (MQ=255)

                     tGTTGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTc                                                                                                                               <  1:1203773/148‑1 (MQ=255)

                      gTTGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCa                                                                                                                              >  1:447047/1‑148 (MQ=255)

                      gTTGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCa                                                                                                                              <  2:2740632/148‑1 (MQ=255)

                      gTTGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCa                                                                                                                              <  2:1885056/148‑1 (MQ=255)

                      gTTGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCa                                                                                                                              <  2:81442/148‑1 (MQ=255)

                       ttGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCAt                                                                                                                             >  2:771246/1‑148 (MQ=255)

                       ttGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCAt                                                                                                                             <  1:1363052/148‑1 (MQ=255)

                       ttGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCAt                                                                                                                             <  1:3069395/148‑1 (MQ=255)

                       ttGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCAt                                                                                                                             >  2:771788/1‑148 (MQ=255)

                        tGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATc                                                                                                                            >  1:3533049/1‑148 (MQ=255)

                        tGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATc                                                                                                                            >  1:1771954/1‑148 (MQ=255)

                             aCTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCgg                                                                                                                       >  2:3388116/1‑148 (MQ=255)

                                ggATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGGCAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGc                                                                                                                    >  2:105957/1‑148 (MQ=255)

                                   ttACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGc                                                                                                                 >  1:1608752/1‑148 (MQ=255)

                                    tACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCt                                                                                                                >  1:2822682/1‑148 (MQ=255)

                                    tACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCt                                                                                                                >  1:1042234/1‑148 (MQ=255)

                                    tACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCt                                                                                                                >  2:1174051/1‑148 (MQ=255)

                                    tACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCt                                                                                                                >  2:2965560/1‑148 (MQ=255)

                                           ggtggtTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGt                                                                                                         >  2:3479387/1‑148 (MQ=255)

                                              ggtTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGcc                                                                                                      >  1:2637283/1‑148 (MQ=255)

                                                tttctttcACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCgg                                                                                                    >  1:1782765/1‑148 (MQ=255)

                                                 ttctttcACCGTGCGTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATAAACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGa                                                                                                   >  2:2787426/1‑148 (MQ=255)

                                                 ttctttcACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGa                                                                                                   <  2:1413558/148‑1 (MQ=255)

                                                 ttctttcACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTAGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGa                                                                                                   <  2:1173584/148‑1 (MQ=255)

                                                 ttctttcACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATACACTCACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGa                                                                                                   >  2:1005999/1‑148 (MQ=255)

                                                    tttcACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGACTACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGc                                                                                                <  2:3498718/148‑1 (MQ=255)

                                                       cACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTa                                                                                             <  1:609869/148‑1 (MQ=255)

                                                           gTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGa                                                                                         <  1:2072701/148‑1 (MQ=255)

                                                            tGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGAc                                                                                        <  1:1109342/148‑1 (MQ=255)

                                                                         gCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGca                                                                           <  2:1453887/148‑1 (MQ=255)

                                                                          ccATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGcat                                                                          <  1:595058/148‑1 (MQ=255)

                                                                              aGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGcatccat                                                                      >  2:413407/1‑148 (MQ=255)

                                                                              aGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGcatccat                                                                      <  2:864450/148‑1 (MQ=255)

                                                                              aGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGcatccat                                                                      >  2:411689/1‑148 (MQ=255)

                                                                               gACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGcatccatc                                                                     <  2:447047/148‑1 (MQ=255)

                                                                               gACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAAATCATCGCCGGGGCCGCTACGACGAGCCGGAAGCGTAATGACCAGCGCATCGGcatccatc                                                                     >  2:1852609/1‑148 (MQ=255)

                                                                                 cAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAg                                                                   >  1:3127719/1‑148 (MQ=255)

                                                                                   gATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAggg                                                                 >  1:3381361/1‑148 (MQ=255)

                                                                                    aTGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCGGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGc                                                                >  1:963143/1‑148 (MQ=255)

                                                                                    aTGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGc                                                                <  1:928935/148‑1 (MQ=255)

                                                                                     tGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCa                                                               <  1:866326/148‑1 (MQ=255)

                                                                                      gTTGAGCTGGTAAACATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCAt                                                              <  2:2673913/148‑1 (MQ=255)

                                                                                      gTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCAt                                                              <  2:271643/148‑1 (MQ=255)

                                                                                          aGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAg                                                          <  1:3019653/148‑1 (MQ=255)

                                                                                           gCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGa                                                         >  2:1945792/1‑148 (MQ=255)

                                                                                               gTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGt                                                     <  2:1095078/148‑1 (MQ=255)

                                                                                                tAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTc                                                    >  1:1799073/1‑148 (MQ=255)

                                                                                                tAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTc                                                    >  1:1799080/1‑148 (MQ=255)

                                                                                                tAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTc                                                    >  1:3408555/1‑148 (MQ=255)

                                                                                                tAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTc                                                    <  1:2146298/148‑1 (MQ=255)

                                                                                                tAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTc                                                    >  1:1966532/1‑148 (MQ=255)

                                                                                                    aataatGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGaa                                                >  2:1855256/1‑148 (MQ=255)

                                                                                                    aataatGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGaa                                                >  2:2705554/1‑148 (MQ=255)

                                                                                                    aataatGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTAGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGaa                                                >  2:252934/1‑148 (MQ=255)

                                                                                                     ataatGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAAt                                               <  2:524180/148‑1 (MQ=255)

                                                                                                        atGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGc                                            >  1:1118287/1‑148 (MQ=255)

                                                                                                        atGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGc                                            >  1:2312849/1‑148 (MQ=255)

                                                                                                             gggAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACt                                       <  1:3019684/148‑1 (MQ=255)

                                                                                                               gAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAg                                     <  1:771246/148‑1 (MQ=255)

                                                                                                               gAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAg                                     <  1:771788/148‑1 (MQ=255)

                                                                                                               gAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAg                                     >  1:2663470/1‑148 (MQ=255)

                                                                                                                   aCGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCa                                 >  2:2902713/1‑148 (MQ=255)

                                                                                                                     gATGGGCCAGCGCGCTATCCACTAACTCTAGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAgg                               <  1:1320312/148‑1 (MQ=255)

                                                                                                                       tGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCt                             <  1:1331098/148‑1 (MQ=255)

                                                                                                                         ggCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTcc                           <  2:98315/148‑1 (MQ=255)

                                                                                                                            cAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATg                        >  2:3296792/1‑148 (MQ=255)

                                                                                                                              gcgcgcTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCg                      >  1:2394891/1‑148 (MQ=255)

                                                                                                                                 cgcTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAg                   <  2:3298185/148‑1 (MQ=255)

                                                                                                                                 cgcTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAg                   >  1:2804403/1‑148 (MQ=255)

                                                                                                                                   cTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCa                 >  1:1087043/1‑148 (MQ=255)

                                                                                                                                   cTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCa                 >  1:539461/1‑148 (MQ=255)

                                                                                                                                     aTCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGa               >  2:112836/1‑148 (MQ=255)

                                                                                                                                     aTCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGa               >  2:112521/1‑148 (MQ=255)

                                                                                                                                     aTCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGa               >  1:2531617/1‑148 (MQ=255)

                                                                                                                                     aTCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGa               >  2:2653191/1‑148 (MQ=255)

                                                                                                                                      tCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGAt              >  1:2457487/1‑148 (MQ=255)

                                                                                                                                         aCTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGc           <  2:1604525/148‑1 (MQ=255)

                                                                                                                                         aCTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGc           <  2:1604714/148‑1 (MQ=255)

                                                                                                                                           tAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTa         >  1:3358033/1‑148 (MQ=255)

                                                                                                                                              ctctTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCa      >  2:3300610/1‑148 (MQ=255)

                                                                                                                                               tctTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCaa     >  1:3167046/1‑148 (MQ=255)

                                                                                                                                                  tGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGc  >  2:1220043/1‑148 (MQ=255)

GTTCTTCTAATACTCGTCCACTGTTGGTTACTGGATTACGCGGGGTGGTTTCTTTCACCGTGCCTTGCGCGTCGCCATAGACAGATGTTGAGCTGGTAAAAATAATGCGGGGAATACGATGGGCCAGCGCGCTATCCACTAACTCTTGTACCGCTTGTAAATAGAACTCATCGCCGGGGCCGCTACGACGTGCCGGAAGCGTAATGACCAGCGCATCGGCATCCATCAGGGCATCCAGATCGTCAGAATCGCAAACTAGCTCAGGCTCCATGCGAAGCAGATAGCTATCAATGC  >  CP009273/2082186‑2082479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`