Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 196,832 C→A 100% I806I (ATC→ATA)  bamA → BamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273196,8320CA95.9% 835.1 / 21.1 281I806I (ATC→ATA) bamABamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor
Reads supporting (aligned to +/- strand):  ref base C (6/6);  new base A (123/146);  total (129/152)
Fisher's exact test for biased strand distribution p-value = 7.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 282 total aligned reads displayed.

CGGACTATAGTGATCCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATCGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAA  >  CP009273/196687‑196976
                                                                                                                                                 |                                                                                                                                                
cGGACTATAGTGATCCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATCgg                                                                                                                                                >  2:3157190/1‑148 (MQ=255)
     tataGTGATCCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTaaaa                                                                                                                                           >  1:1246872/1‑148 (MQ=255)
      ataGTGATCCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAAc                                                                                                                                          <  2:2480397/148‑1 (MQ=255)
      ataGTGATCCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAAc                                                                                                                                          <  1:2630179/148‑1 (MQ=255)
             tCCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTaa                                                                                                                                   >  2:791862/1‑148 (MQ=255)
             tCCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTaa                                                                                                                                   >  2:2902485/1‑148 (MQ=255)
               cAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAgt                                                                                                                                 <  2:927227/148‑1 (MQ=255)
                aaGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATCGGTAAAACCTGGTAAgtg                                                                                                                                <  2:2101047/148‑1 (MQ=255)
                     atatCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTtctc                                                                                                                           <  1:3489344/148‑1 (MQ=255)
                     atatCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTtctc                                                                                                                           <  1:3414693/148‑1 (MQ=255)
                      tatCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTcc                                                                                                                          >  2:2076512/1‑148 (MQ=255)
                      tatCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTcc                                                                                                                          <  1:3279915/148‑1 (MQ=255)
                       atCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAAACTGGTAAGTGTTCTCca                                                                                                                         >  1:786077/1‑148 (MQ=255)
                        tCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCcac                                                                                                                        >  2:2406430/1‑148 (MQ=255)
                           gTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCCCaaa                                                                                                                     >  1:753391/1‑148 (MQ=255)
                           gTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACaaa                                                                                                                     >  2:1421201/1‑148 (MQ=255)
                           gTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCACCTAAGCCCAGTCGTTCAAAAAATACGATGGAGACAAAGCACAACAATTACAATTTAACAAAGGTAAAAACACGTAATTGTTCTACACaaa                                                                                                                     >  1:2999685/1‑148 (MQ=255)
                            tATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGGACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCGGGTACGTGGTCTCcacccgg                                                                                                                    >  1:1922591/1‑144 (MQ=255)
                             aTGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAgg                                                                                                                   >  2:1951957/1‑148 (MQ=255)
                                tCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTGGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAAt                                                                                                                >  1:22387/1‑148 (MQ=255)
                                     gggTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATgtagt                                                                                                           <  2:592614/148‑1 (MQ=255)
                                     gggTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATgtagt                                                                                                           <  1:1449224/148‑1 (MQ=255)
                                       gTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGATACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATgtagtgg                                                                                                         >  1:269529/1‑148 (MQ=255)
                                       gTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATgtagtgg                                                                                                         <  2:2595578/148‑1 (MQ=255)
                                       gTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATgtagtgg                                                                                                         <  2:540160/148‑1 (MQ=255)
                                       gTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACCATGGATACAAGGCAGAACAGTTCCAGTTTAAAATAGGTAAAACCAGGTAAGTGTTATCCACAAAGGAATgtagtgg                                                                                                         >  1:643138/1‑148 (MQ=255)
                                        tATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATgtagtggt                                                                                                        <  2:2948848/148‑1 (MQ=255)
                                        tATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATgtagtggt                                                                                                        <  1:3292576/148‑1 (MQ=255)
                                          tCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATgtagtggtag                                                                                                      >  2:2504404/1‑148 (MQ=255)
                                          tCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTAAGATGGAGAAAAGGCAGAACAGTTCAAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATgtagtggtag                                                                                                      >  1:1318813/1‑148 (MQ=255)
                                                tACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGc                                                                                                >  2:3051333/1‑148 (MQ=255)
                                                  cAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGa                                                                                              >  1:86007/1‑148 (MQ=255)
                                                  cAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGa                                                                                              <  1:1686403/148‑1 (MQ=255)
                                                   aaTGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGAt                                                                                             <  2:713850/148‑1 (MQ=255)
                                                    aTGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGAAAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTTGTGTAGCGATg                                                                                            <  1:1753645/148‑1 (MQ=255)
                                                     tGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGa                                                                                           >  2:3525207/1‑148 (MQ=255)
                                                     tGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGa                                                                                           <  2:1521632/148‑1 (MQ=255)
                                                       gATGTCCCCCTTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACt                                                                                         <  2:3082179/148‑1 (MQ=255)
                                                       gATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACt                                                                                         <  2:1246872/148‑1 (MQ=255)
                                                       gATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACt                                                                                         <  2:1597021/148‑1 (MQ=255)
                                                       gATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACt                                                                                         <  2:63866/148‑1 (MQ=255)
                                                         tGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACttt                                                                                       <  1:1464744/148‑1 (MQ=255)
                                                          gTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTa                                                                                      <  1:3502891/148‑1 (MQ=255)
                                                           tCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACACTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTACACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAg                                                                                     >  2:3148783/1‑148 (MQ=255)
                                                              ccATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCg                                                                                  >  2:2672980/1‑148 (MQ=255)
                                                              ccATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCg                                                                                  <  1:1149617/148‑1 (MQ=255)
                                                               cATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGa                                                                                 <  2:2563207/148‑1 (MQ=255)
                                                                aTTGGGGCCGTTGGTGTTCTCCTTCGCCCAGCCGTTCAAACAGTACGATGGTGACAAGGCAGAACAGTTCCAGTTTAACATCGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGAt                                                                                <  1:1406020/148‑1 (MQ=255)
                                                                  tGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCa                                                                              <  1:1800758/148‑1 (MQ=255)
                                                                   ggggCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCaa                                                                             <  1:2030417/148‑1 (MQ=255)
                                                                        cGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATaa                                                                        >  2:1988067/1‑148 (MQ=255)
                                                                          ttGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATCGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGa                                                                      >  2:1201872/1‑148 (MQ=255)
                                                                          ttGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGa                                                                      >  1:1370462/1‑148 (MQ=255)
                                                                                  ctcCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCggg                                                              >  2:2830559/1‑148 (MQ=255)
                                                                                   tcCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGc                                                             <  2:1334413/148‑1 (MQ=255)
                                                                                   tcCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGc                                                             <  2:2433212/148‑1 (MQ=255)
                                                                                     cTACGCCCAGCCGTTAAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCATTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATAAATATAAGATCGCCGGGCCa                                                           <  1:2461614/148‑1 (MQ=255)
                                                                                            cAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAg                                                    >  1:2318924/1‑148 (MQ=255)
                                                                                                cGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACt                                                <  2:2619176/148‑1 (MQ=255)
                                                                                                  ttCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGc                                              <  1:2841843/148‑1 (MQ=255)
                                                                                                   tCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCa                                             >  1:2747182/1‑148 (MQ=255)
                                                                                                    cAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCAc                                            >  1:1040926/1‑148 (MQ=255)
                                                                                                    cAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCAc                                            <  2:3355903/148‑1 (MQ=255)
                                                                                                      aaaaGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCAccc                                          >  1:1898116/1‑148 (MQ=255)
                                                                                                      aaaaGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCAccc                                          >  2:2022454/1‑148 (MQ=255)
                                                                                                       aaaGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCt                                         <  2:22387/148‑1 (MQ=255)
                                                                                                       aaaGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCt                                         >  2:865003/1‑148 (MQ=255)
                                                                                                       aaaGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCt                                         <  2:700623/148‑1 (MQ=255)
                                                                                                       aaaGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCt                                         >  1:995279/1‑148 (MQ=255)
                                                                                                        caGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTc                                        <  2:3503666/147‑1 (MQ=255)
                                                                                                        aaGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTc                                        >  2:138229/1‑148 (MQ=255)
                                                                                                         aGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATCGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTcc                                       <  2:3019794/148‑1 (MQ=255)
                                                                                                         aGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTcc                                       >  1:1029385/1‑148 (MQ=255)
                                                                                                           tACGATGGCGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCgg                                     <  1:2144107/148‑1 (MQ=255)
                                                                                                            aCGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGt                                    >  1:1874356/1‑148 (MQ=255)
                                                                                                             cGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTg                                   <  2:1791167/148‑1 (MQ=255)
                                                                                                                tGGAGACAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCaa                                >  2:2350650/1‑148 (MQ=255)
                                                                                                                  gagaCAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAAt                              >  1:1994578/1‑148 (MQ=255)
                                                                                                                   agaCAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATg                             <  2:3458686/148‑1 (MQ=255)
                                                                                                                    gaaaatgCAGAACAGTTCCAGTTTAACATCGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATgg                            <  1:1637455/142‑1 (MQ=255)
                                                                                                                      cAAGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGa                          <  1:882725/148‑1 (MQ=255)
                                                                                                                       aaGGCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGAt                         <  1:1674807/148‑1 (MQ=255)
                                                                                                                          gCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGt                      <  1:1786256/148‑1 (MQ=255)
                                                                                                                          gCAGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGt                      <  2:2492867/148‑1 (MQ=255)
                                                                                                                           cAGACCAGGTCCAGTTCAACATCGCTAAAACCGGGTAAGTGTTCTCCACCCAGGAAGGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGAACGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTa                     <  2:666829/148‑1 (MQ=255)
                                                                                                                            aGAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTaa                    <  2:1091697/148‑1 (MQ=255)
                                                                                                                             gAACAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAg                   <  2:3401241/148‑1 (MQ=255)
                                                                                                                              aaCAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAgg                  <  2:3056189/148‑1 (MQ=255)
                                                                                                                              aaCAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAgg                  <  1:3051354/148‑1 (MQ=255)
                                                                                                                               aCAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGa                 >  1:2701430/1‑148 (MQ=255)
                                                                                                                                cAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAg                <  1:2973474/148‑1 (MQ=255)
                                                                                                                                cAGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAg                >  2:1312596/1‑148 (MQ=255)
                                                                                                                                 aGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGt               <  1:2902485/148‑1 (MQ=255)
                                                                                                                                 aGTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGt               <  1:423868/148‑1 (MQ=255)
                                                                                                                                  gTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGtt              <  1:853854/148‑1 (MQ=255)
                                                                                                                                  gTTCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGtt              >  1:1604941/1‑148 (MQ=255)
                                                                                                                                    tCCAGTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTa            <  2:1029733/148‑1 (MQ=255)
                                                                                                                                        gTTTAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATtgt        <  2:329580/148‑1 (MQ=255)
                                                                                                                                         tttAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATtgtg       >  1:3005769/1‑148 (MQ=255)
                                                                                                                                          ttAACATAGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGa      >  2:184792/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CGGACTATAGTGATCCAAGCAATATCCGTATGTCTGCGGGTATCGCATTACAATGGATGTCCCCATTGGGGCCGTTGGTGTTCTCCTACGCCCAGCCGTTCAAAAAGTACGATGGAGACAAGGCAGAACAGTTCCAGTTTAACATCGGTAAAACCTGGTAAGTGTTCTCCACAAAGGAATGTAGTGGTAGTGTAGCGATGACTTTAGGCGATCAATATAAGATCGCCGGGCCACGCAAAGAACTGCACCCTCCGGTGCAAATGGGATGGTAAGGAGTTTATTGTGAAAAA  >  CP009273/196687‑196976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑