Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,141,613 G→T 62.0% V153V (GTG→GTT)  yegD → Hsp70 chaperone family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,141,6130GT62.0% 76.2 / 249.1 192V153V (GTG→GTT) yegDHsp70 chaperone family protein
Reads supporting (aligned to +/- strand):  ref base G (34/39);  new base T (64/55);  total (98/94)
Fisher's exact test for biased strand distribution p-value = 3.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.24e-01

Only 100 of 193 total aligned reads displayed.

GTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGAT  >  CP009273/2141466‑2141754
                                                                                                                                                   |                                                                                                                                             
gTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTg                                                                                                                                               <  2:96073/148‑1 (MQ=255)
   tgtgAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTAtt                                                                                                                                            <  1:1578461/148‑1 (MQ=255)
   tgtgAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGAtt                                                                                                                                            <  2:2039097/148‑1 (MQ=255)
   tgtgAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGAtt                                                                                                                                            <  2:2269275/148‑1 (MQ=255)
     tgAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTgg                                                                                                                                          >  2:1072297/1‑148 (MQ=255)
     tgAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTgg                                                                                                                                          <  2:1494935/148‑1 (MQ=255)
     tgAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTgg                                                                                                                                          >  2:2604541/1‑148 (MQ=255)
             ccAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGTGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGa                                                                                                                                  <  2:428719/148‑1 (MQ=255)
             ccAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGa                                                                                                                                  <  1:9697/148‑1 (MQ=255)
              cAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGAt                                                                                                                                 >  2:576619/1‑148 (MQ=255)
              cAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGAt                                                                                                                                 >  2:3241380/1‑148 (MQ=255)
               aaaaTCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATc                                                                                                                                >  2:948595/1‑148 (MQ=255)
                 aaTCGGTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCaa                                                                                                                              <  1:2150870/148‑1 (MQ=255)
                  aTCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAAc                                                                                                                             <  2:210340/148‑1 (MQ=255)
                   tCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACt                                                                                                                            >  2:3311241/1‑148 (MQ=255)
                    cGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACtt                                                                                                                           <  2:2411334/148‑1 (MQ=255)
                      ttCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTcc                                                                                                                         <  1:2873548/148‑1 (MQ=255)
                      gtCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTcc                                                                                                                         <  2:2917220/147‑1 (MQ=255)
                        ccTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAg                                                                                                                       <  2:167723/148‑1 (MQ=255)
                         cTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAgg                                                                                                                      <  2:2428264/148‑1 (MQ=255)
                          tCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAggg                                                                                                                     <  1:1376395/148‑1 (MQ=255)
                          tCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAggg                                                                                                                     <  1:318987/148‑1 (MQ=255)
                             gTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCt                                                                                                                  >  2:455815/1‑148 (MQ=255)
                             gTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCt                                                                                                                  >  2:1163/1‑148 (MQ=255)
                              tGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTg                                                                                                                 <  1:2165209/148‑1 (MQ=255)
                                 cAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGc                                                                                                              >  1:3074919/1‑148 (MQ=255)
                                   gCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCgg                                                                                                            >  2:2702396/1‑148 (MQ=255)
                                   gCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCgg                                                                                                            >  2:1396688/1‑148 (MQ=255)
                                        ttAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGtgatg                                                                                                       >  1:2818896/1‑148 (MQ=255)
                                            aaCCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGc                                                                                                   >  1:2049799/1‑148 (MQ=255)
                                             aCCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCa                                                                                                  >  2:701737/1‑148 (MQ=255)
                                             aCCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCa                                                                                                  >  2:771864/1‑148 (MQ=255)
                                              ccGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCaa                                                                                                 >  1:3522427/1‑148 (MQ=255)
                                               cGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCaaa                                                                                                <  1:3468453/148‑1 (MQ=255)
                                               cGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCaaa                                                                                                <  1:948595/148‑1 (MQ=255)
                                                 cagcagGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAaca                                                                                              <  2:2964337/148‑1 (MQ=255)
                                                   gcagGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACAcc                                                                                            >  2:3137598/1‑148 (MQ=255)
                                                   gcagGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACAcc                                                                                            >  1:1063610/1‑148 (MQ=255)
                                                    cagGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACAccc                                                                                           <  2:3330397/148‑1 (MQ=255)
                                                    cagGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACAccc                                                                                           <  2:2932367/148‑1 (MQ=255)
                                                          gcgcTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGgcgc                                                                                     >  1:2786915/1‑148 (MQ=255)
                                                          gcgcTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGgcgc                                                                                     >  1:2886090/1‑148 (MQ=255)
                                                           cgcTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCa                                                                                    >  1:44818/1‑148 (MQ=255)
                                                             cTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAg                                                                                  >  1:2801436/1‑148 (MQ=255)
                                                              tGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAgg                                                                                 >  2:930172/1‑148 (MQ=255)
                                                                tttGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGa                                                                               <  2:2083377/148‑1 (MQ=255)
                                                                tttGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGa                                                                               <  2:1193530/148‑1 (MQ=255)
                                                                tttGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGa                                                                               >  2:2312757/1‑148 (MQ=255)
                                                                 ttGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGAt                                                                              >  1:3262322/1‑148 (MQ=255)
                                                                  tGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGAtt                                                                             <  1:900319/148‑1 (MQ=255)
                                                                   gAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTc                                                                            <  2:3197345/148‑1 (MQ=255)
                                                                   gAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTc                                                                            <  2:3378674/148‑1 (MQ=255)
                                                                   gAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTc                                                                            >  2:1133108/1‑148 (MQ=255)
                                                                    aGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCt                                                                           >  2:2193687/1‑148 (MQ=255)
                                                                    aGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCt                                                                           <  1:306665/148‑1 (MQ=255)
                                                                    aGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCt                                                                           >  2:2737493/1‑148 (MQ=255)
                                                                    aGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGAGGAAGCAAACACCCAGGCGCAAGGGATTCt                                                                           >  2:533560/1‑148 (MQ=255)
                                                                     ggATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTg                                                                          >  1:836525/1‑148 (MQ=255)
                                                                      gATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTgg                                                                         >  1:1908812/1‑148 (MQ=255)
                                                                               tGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCgcgg                                                                <  2:2801473/148‑1 (MQ=255)
                                                                                gcgcAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCgcggc                                                               >  2:2882580/1‑148 (MQ=255)
                                                                                gcgcAATGATGTTGCACATTCGCCAGCAGGCGCAGGAACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCAGGGCGGTGAGGACGCAAACACCCAGGCGCACGGGATGCTGGAACGCgcggc                                                               >  2:2215574/1‑148 (MQ=255)
                                                                                 cgcAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCgcggcg                                                              >  1:1978156/1‑148 (MQ=255)
                                                                                  gcAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGa                                                             <  1:3335141/148‑1 (MQ=255)
                                                                                   cAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGaa                                                            >  2:424882/1‑148 (MQ=255)
                                                                                   cAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGaa                                                            >  1:220784/1‑148 (MQ=255)
                                                                                     atgatgTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGc                                                          >  2:1225087/1‑148 (MQ=255)
                                                                                      tgatgTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCg                                                         >  1:2975311/1‑148 (MQ=255)
                                                                                      tgatgTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGAGGGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCg                                                         <  1:3137598/148‑1 (MQ=255)
                                                                                        atgTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTg                                                       <  1:3250893/148‑1 (MQ=255)
                                                                                        atgTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTg                                                       >  1:538052/1‑148 (MQ=255)
                                                                                          gTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGCCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGcc                                                     >  2:1785355/1‑148 (MQ=255)
                                                                                               acaTTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGAtt                                                <  2:653762/148‑1 (MQ=255)
                                                                                               acaTTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGAtt                                                >  2:1258657/1‑148 (MQ=255)
                                                                                                  ttCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAg                                             <  1:636018/148‑1 (MQ=255)
                                                                                                  ttCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAg                                             >  1:2102776/1‑148 (MQ=255)
                                                                                                          caggcgcagacACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGACGCGTGCCGGAGTCAGGGACGTgg                                     >  2:1755016/1‑148 (MQ=255)
                                                                                                             gcgcaggcACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTAt                                  >  2:350475/1‑148 (MQ=255)
                                                                                                              cgcaggcACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGCAGCGTGCCGGATTCAGGGACGTGGTAtt                                 <  2:247225/148‑1 (MQ=255)
                                                                                                               gcaggcACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTc                                <  1:2136140/148‑1 (MQ=255)
                                                                                                                caggcACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTcc                               <  2:1552012/148‑1 (MQ=255)
                                                                                                                   gcACAGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGt                            >  1:1136940/1‑148 (MQ=255)
                                                                                                                    cacaGCTGCCAGAAGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTa                           <  1:1043321/148‑1 (MQ=255)
                                                                                                                         cTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGc                      >  2:1001318/1‑148 (MQ=255)
                                                                                                                                aaGCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTcgc               >  2:1615663/1‑148 (MQ=255)
                                                                                                                                aaGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTcgc               <  1:2423070/148‑1 (MQ=255)
                                                                                                                                  gCGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCgg             >  2:1336466/1‑148 (MQ=255)
                                                                                                                                   cGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCggc            <  1:1485487/148‑1 (MQ=255)
                                                                                                                                   cGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCggc            <  1:1485188/148‑1 (MQ=255)
                                                                                                                                   cGATTACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCggc            >  1:2528774/1‑148 (MQ=255)
                                                                                                                                     aTTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCggctg          <  2:3519443/148‑1 (MQ=255)
                                                                                                                                       tACTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCggctggg        >  1:1066972/1‑148 (MQ=255)
                                                                                                                                       tACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCggctggg        >  1:1686102/1‑148 (MQ=255)
                                                                                                                                       tACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCggctggg        >  1:1656243/1‑148 (MQ=255)
                                                                                                                                         cTCAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCggctgggct      <  2:832978/148‑1 (MQ=255)
                                                                                                                                           cAGGCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGgctgggctgg    <  2:1871614/148‑1 (MQ=255)
                                                                                                                                           cAGGCGGTGATTGGTCGTCCGATCAACTACCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGgctgggctgg    >  1:864727/1‑148 (MQ=255)
                                                                                                                                             ggCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGAt  >  1:397224/1‑148 (MQ=255)
                                                                                                                                             ggCGGTTATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGAt  <  2:705049/148‑1 (MQ=255)
                                                                                                                                             ggCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGAt  >  2:2520964/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
GTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGAT  >  CP009273/2141466‑2141754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑