Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,194,695 T→A 100% L313Q (CTA→CAA)  yehI → DUF4132 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,194,6950TA99.6% 747.9 / ‑4.7 225L313Q (CTA→CAA) yehIDUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (101/123);  total (101/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 225 total aligned reads displayed.

AACCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAG  >  CP009273/2194550‑2194838
                                                                                                                                                 |                                                                                                                                               
aaCCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAAc                                                                                                                                               >  2:2668747/1‑148 (MQ=255)
aaCCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGACAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAAc                                                                                                                                               >  2:3094589/1‑148 (MQ=255)
 aCCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACt                                                                                                                                              >  2:2754408/1‑148 (MQ=255)
   cACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGACATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACtgg                                                                                                                                            >  2:3434431/1‑148 (MQ=255)
     cTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACtggtg                                                                                                                                          <  2:2172028/148‑1 (MQ=255)
     cTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACtggtg                                                                                                                                          >  1:1108127/1‑148 (MQ=255)
      ttCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACtggtgg                                                                                                                                         <  1:925474/148‑1 (MQ=255)
      ttCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACtggtgg                                                                                                                                         <  1:2829211/148‑1 (MQ=255)
       tCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGc                                                                                                                                        >  1:148915/1‑148 (MQ=255)
          gCTACGCGGCCTGCGCCTCCCTTGTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGTCAGTCTGCTGGTACACATTAACCATCCTGAAGTCATCGGCACCTTGCAACTGGTGGCTGa                                                                                                                                     <  2:3310544/148‑1 (MQ=255)
          gCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGa                                                                                                                                     >  1:1211681/1‑148 (MQ=255)
          gCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGa                                                                                                                                     >  1:2861689/1‑148 (MQ=255)
           cTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGAt                                                                                                                                    >  2:1410352/1‑148 (MQ=255)
              cgcgGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATaaa                                                                                                                                 >  1:275657/1‑148 (MQ=255)
               gcgGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATaaaa                                                                                                                                <  1:3441475/148‑1 (MQ=255)
                cgGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATaaaaa                                                                                                                               <  1:297751/148‑1 (MQ=255)
                  gCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAaaaca                                                                                                                             <  1:1716731/148‑1 (MQ=255)
                     tGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAaaacaaac                                                                                                                          >  1:2184515/1‑148 (MQ=255)
                      gcgcCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAAcc                                                                                                                         <  2:67518/148‑1 (MQ=255)
                            cccTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCt                                                                                                                   >  2:296033/1‑148 (MQ=255)
                              cTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGc                                                                                                                 >  1:673402/1‑148 (MQ=255)
                              cTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGc                                                                                                                 >  1:2461502/1‑148 (MQ=255)
                               ttttGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCa                                                                                                                <  2:1467477/148‑1 (MQ=255)
                               ttttGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCa                                                                                                                <  1:208037/148‑1 (MQ=255)
                                     gcgAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACgtgt                                                                                                          <  2:2045736/148‑1 (MQ=255)
                                     gcgAACAAGGACTGGCAGCCAGTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACgtgt                                                                                                          <  2:2045659/148‑1 (MQ=255)
                                       gAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAg                                                                                                        <  1:2924663/148‑1 (MQ=255)
                                       gAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAg                                                                                                        <  1:2594715/148‑1 (MQ=255)
                                       gAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAg                                                                                                        >  2:923099/1‑148 (MQ=255)
                                           aaGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTaa                                                                                                    >  2:784509/1‑148 (MQ=255)
                                            aGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTaaa                                                                                                   >  2:783124/1‑148 (MQ=255)
                                             ggACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAAt                                                                                                  <  1:2853547/148‑1 (MQ=255)
                                              gACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATa                                                                                                 <  2:829779/148‑1 (MQ=255)
                                              gACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATa                                                                                                 >  1:42159/1‑148 (MQ=255)
                                               aCTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATAc                                                                                                >  1:2395422/1‑148 (MQ=255)
                                                  ggCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCAt                                                                                             >  2:1568379/1‑148 (MQ=255)
                                                   gCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATa                                                                                            >  2:2999575/1‑148 (MQ=255)
                                                    cAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATaa                                                                                           <  2:3526779/148‑1 (MQ=255)
                                                    cAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATaa                                                                                           <  2:1868500/148‑1 (MQ=255)
                                                         aTTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATCAAAACt                                                                                      >  2:3511456/1‑148 (MQ=255)
                                                         aTTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACt                                                                                      <  2:66037/148‑1 (MQ=255)
                                                          ttCCGCGCCTCGCGATGTATGCCCCTAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCCAGTCATCCGCACCTTGCAACTGGTGGCTGTTCAAAACAGACCCAGCCTGCAACGTGTCGCGAACTCGCATAAAACCtt                                                                                     >  2:2522603/1‑148 (MQ=255)
                                                          ttCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACtt                                                                                     >  2:272976/1‑148 (MQ=255)
                                                           tCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTc                                                                                    >  1:3554298/1‑148 (MQ=255)
                                                            ccGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTcc                                                                                   <  1:2668747/148‑1 (MQ=255)
                                                              gcgcCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTcccc                                                                                 <  1:1066097/148‑1 (MQ=255)
                                                              gcgcCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTcccc                                                                                 <  2:2809130/148‑1 (MQ=255)
                                                               cgcCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTccccc                                                                                <  1:1781547/148‑1 (MQ=255)
                                                                 ccTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCAt                                                                              >  1:1859421/1‑148 (MQ=255)
                                                                  cTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATg                                                                             <  2:2304186/148‑1 (MQ=255)
                                                                    cgcgATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCg                                                                           <  2:1509731/148‑1 (MQ=255)
                                                                     gcgATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGa                                                                          <  1:128237/148‑1 (MQ=255)
                                                                     gcgATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGa                                                                          <  2:3325928/148‑1 (MQ=255)
                                                                     gcgATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGa                                                                          <  1:3434431/148‑1 (MQ=255)
                                                                         tGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCt                                                                      >  2:1128277/1‑148 (MQ=255)
                                                                          gTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTc                                                                     >  2:1621754/1‑148 (MQ=255)
                                                                                ccATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCa                                                               <  1:864402/148‑1 (MQ=255)
                                                                                ccATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCa                                                               <  1:138535/148‑1 (MQ=255)
                                                                                ccATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCCCGCCGCa                                                               <  1:1843937/148‑1 (MQ=255)
                                                                                 cATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCAc                                                              >  2:488041/1‑148 (MQ=255)
                                                                                 cATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCAAAAAAACTTCCCCCATGCGACGCTCGCCGCAc                                                              >  1:570281/1‑148 (MQ=255)
                                                                                  aTAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACt                                                             <  1:820468/148‑1 (MQ=255)
                                                                                    aaaGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTgg                                                           >  1:1005748/1‑148 (MQ=255)
                                                                                     aagaagATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGc                                                          <  2:1288876/148‑1 (MQ=255)
                                                                                     aagaagATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGc                                                          >  1:1042144/1‑148 (MQ=255)
                                                                                         agaTTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGaa                                                      <  2:2586674/148‑1 (MQ=255)
                                                                                         agaTTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGaa                                                      <  2:585315/148‑1 (MQ=255)
                                                                                           aTTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTCGCTAAATACCATAAAAACTTCCCCCCTGCGACGCTCGCCGCACTGGCAGAAct                                                    >  2:2561303/1‑148 (MQ=255)
                                                                                           aTTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGaa                                                      >  1:1167823/1‑146 (MQ=255)
                                                                                           aTTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGaa                                                      <  2:1167509/146‑1 (MQ=255)
                                                                                           aTTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAAct                                                    >  1:491876/1‑148 (MQ=255)
                                                                                             tGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAActgc                                                  <  2:1166049/148‑1 (MQ=255)
                                                                                               cGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAActgctg                                                <  2:2276582/148‑1 (MQ=255)
                                                                                               cGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATACGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAActgctg                                                >  1:3005285/1‑148 (MQ=255)
                                                                                                  cAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCg                                             >  1:1975349/1‑148 (MQ=255)
                                                                                                  cAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCg                                             >  1:174262/1‑148 (MQ=255)
                                                                                                       tgctgGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAAc                                        <  1:1920298/148‑1 (MQ=255)
                                                                                                          tgGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGa                                     <  1:1463779/148‑1 (MQ=255)
                                                                                                           ggTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGaa                                    <  2:2995267/148‑1 (MQ=255)
                                                                                                           ggTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGaa                                    <  2:1220243/148‑1 (MQ=255)
                                                                                                              aCAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAacca                                 >  1:3052129/1‑148 (MQ=255)
                                                                                                                   ttAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgc                            >  2:1253188/1‑148 (MQ=255)
                                                                                                                          tCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCt                     >  1:2355520/1‑148 (MQ=255)
                                                                                                                          tCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCt                     <  2:1815074/148‑1 (MQ=255)
                                                                                                                          tCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCt                     <  2:3207624/148‑1 (MQ=255)
                                                                                                                          tCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCt                     <  2:3208164/148‑1 (MQ=255)
                                                                                                                               aaGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTa                <  2:3565849/148‑1 (MQ=255)
                                                                                                                                aGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTAt               >  1:2269978/1‑148 (MQ=255)
                                                                                                                                  tCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATcc             >  1:3199368/1‑148 (MQ=255)
                                                                                                                                   cATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCa            >  1:2713129/1‑148 (MQ=255)
                                                                                                                                   cATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCa            >  1:2880039/1‑148 (MQ=255)
                                                                                                                                    aTCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCaa           <  2:275664/148‑1 (MQ=255)
                                                                                                                                      ccGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAatc         >  1:2507834/1‑148 (MQ=255)
                                                                                                                                       cGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCCGCCCGCCCTGGTTATCCAatca        >  1:2300585/1‑148 (MQ=255)
                                                                                                                                        gCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAatcat       <  1:2148874/148‑1 (MQ=255)
                                                                                                                                          aCCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCg     >  1:3471362/1‑148 (MQ=255)
                                                                                                                                           ccTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGa    <  1:1613732/148‑1 (MQ=255)
                                                                                                                                            cTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGaa   <  2:1107053/148‑1 (MQ=255)
                                                                                                                                             ttGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGACCTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAg  >  2:3115882/1‑148 (MQ=255)
                                                                                                                                             ttGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAg  <  1:1581582/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
AACCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAG  >  CP009273/2194550‑2194838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑