BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,277,743	A→T	27.9%	N46Y (AAC→TAC)	yejL →	UPF0352 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,277,743	0	A	T	27.9%	441.5 / 212.2	254	N46Y (AAC→TAC)	yejL	UPF0352 family protein
Reads supporting (aligned to +/- strand): ref base A (81/102); new base T (28/43); total (109/145)
Fisher's exact test for biased strand distribution p-value = 5.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 254 total aligned reads displayed.

ATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGT  >  CP009273/2277598‑2277889

 tttCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTaca                                                                                                                                                 >  1:2491943/1‑148 (MQ=255)

 tttCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAaca                                                                                                                                                 <  2:3556869/148‑1 (MQ=255)

  ttCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTacac                                                                                                                                                >  1:1018289/1‑148 (MQ=255)

    cGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCa                                                                                                                                              >  2:1422630/1‑148 (MQ=255)

      aTTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGc                                                                                                                                            <  1:1776080/148‑1 (MQ=255)

       tttATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCa                                                                                                                                           <  2:2004889/148‑1 (MQ=255)

         tATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCAtt                                                                                                                                         <  1:2030421/148‑1 (MQ=255)

           tGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGc                                                                                                                                       >  2:2632353/1‑148 (MQ=255)

                caAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCgg                                                                                                                                  >  1:1958615/1‑148 (MQ=255)

                 aaaTTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGc                                                                                                                                 <  1:2435129/148‑1 (MQ=255)

                  aaTTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGcc                                                                                                                                >  1:1610313/1‑148 (MQ=255)

                    tttCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCa                                                                                                                              <  2:557521/148‑1 (MQ=255)

                    tgtCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCa                                                                                                                              <  2:3133141/146‑1 (MQ=255)

                       cccGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACg                                                                                                                           <  2:1358521/148‑1 (MQ=255)

                        ccGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGc                                                                                                                          <  2:1711917/148‑1 (MQ=255)

                         cGCTACAGTGATGAACAGGTTGAACACTTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGcc                                                                                                                         <  2:280914/148‑1 (MQ=255)

                         cGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGcc                                                                                                                         <  2:3241685/148‑1 (MQ=255)

                         cGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGcc                                                                                                                         >  2:1556648/1‑148 (MQ=255)

                         cGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGcc                                                                                                                         >  2:186167/1‑148 (MQ=255)

                              cAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCa                                                                                                                    <  1:1297098/148‑1 (MQ=255)

                              cAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCa                                                                                                                    <  1:1306912/148‑1 (MQ=255)

                              cAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCa                                                                                                                    <  1:2604508/148‑1 (MQ=255)

                                gTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAAt                                                                                                                  <  2:417794/148‑1 (MQ=255)

                                   atgaACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGc                                                                                                               <  2:1724194/148‑1 (MQ=255)

                                      aaCAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTACCaa                                                                                                            >  1:1996687/1‑148 (MQ=255)

                                       aCAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAAc                                                                                                           >  1:1182373/1‑148 (MQ=255)

                                        cAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAAct                                                                                                          <  2:1233210/148‑1 (MQ=255)

                                         aGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAActc                                                                                                         >  2:1233829/1‑148 (MQ=255)

                                          ggTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAActct                                                                                                        <  1:463687/148‑1 (MQ=255)

                                           gTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCtt                                                                                                       <  2:3097203/148‑1 (MQ=255)

                                             tGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCtttt                                                                                                     <  1:1916361/148‑1 (MQ=255)

                                                aCAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGcc                                                                                                  >  2:3336691/1‑148 (MQ=255)

                                                  aaTTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCcg                                                                                                <  1:3134461/148‑1 (MQ=255)

                                                   aTTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCcgc                                                                                               >  2:2224868/1‑148 (MQ=255)

                                                    ttGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCcgcg                                                                                              <  1:2866455/148‑1 (MQ=255)

                                                    ttGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCcgcg                                                                                              >  1:579558/1‑148 (MQ=255)

                                                     tGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCgcgc                                                                                             <  1:1719940/148‑1 (MQ=255)

                                                      gCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGcc                                                                                            >  1:3189772/1‑148 (MQ=255)

                                                          gCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTAc                                                                                        <  2:651932/148‑1 (MQ=255)

                                                          gCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTAc                                                                                        <  2:2439143/148‑1 (MQ=255)

                                                          gCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTAc                                                                                        <  2:2778924/148‑1 (MQ=255)

                                                              agCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTc                                                                                    >  1:2534549/1‑148 (MQ=255)

                                                              agCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTc                                                                                    <  2:2059575/148‑1 (MQ=255)

                                                              agCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTc                                                                                    >  2:1074714/1‑148 (MQ=255)

                                                                ctgctCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCct                                                                                  >  2:277568/1‑148 (MQ=255)

                                                                 tgctCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCctc                                                                                 <  1:3231416/148‑1 (MQ=255)

                                                                  gctCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCctct                                                                                <  1:475684/148‑1 (MQ=255)

                                                                   ctCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTa                                                                               <  2:1617066/148‑1 (MQ=255)

                                                                      aaCGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCa                                                                            >  2:2825733/1‑148 (MQ=255)

                                                                       aCGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCaa                                                                           >  1:2178588/1‑148 (MQ=255)

                                                                        cGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAAc                                                                          >  1:2359726/1‑148 (MQ=255)

                                                                          tACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGa                                                                        <  2:1018289/148‑1 (MQ=255)

                                                                          tACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGa                                                                        <  1:1154825/148‑1 (MQ=255)

                                                                             tGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGa                                                                     >  2:1449670/1‑148 (MQ=255)

                                                                                         aGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTa                                                         <  1:167159/148‑1 (MQ=255)

                                                                                          ggCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTaa                                                        >  1:1065132/1‑148 (MQ=255)

                                                                                           gCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAg                                                       <  2:2126402/148‑1 (MQ=255)

                                                                                           gCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAg                                                       <  2:329647/148‑1 (MQ=255)

                                                                                            cTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAgg                                                      <  2:472925/148‑1 (MQ=255)

                                                                                             tCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAggg                                                     <  1:1372707/148‑1 (MQ=255)

                                                                                             tCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTATTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAggg                                                     <  2:826489/148‑1 (MQ=255)

                                                                                                gaccgaccTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGaaa                                                  <  2:1954380/148‑1 (MQ=255)

                                                                                                gaccgaccTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGaaa                                                  >  1:905309/1‑148 (MQ=255)

                                                                                                gaccgaccTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGaaa                                                  <  1:2632353/148‑1 (MQ=255)

                                                                                                  ccgaccTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACa                                                >  2:1766954/1‑148 (MQ=255)

                                                                                                   cgaccTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAg                                               <  2:2047354/148‑1 (MQ=255)

                                                                                                      ccTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATa                                            <  2:1843648/148‑1 (MQ=255)

                                                                                                        tttCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAAc                                          >  2:3376069/1‑148 (MQ=255)

                                                                                                           cccTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAgg                                       <  2:2491943/148‑1 (MQ=255)

                                                                                                             cTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGtt                                     <  2:906731/148‑1 (MQ=255)

                                                                                                               gATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTAt                                   <  1:1344421/148‑1 (MQ=255)

                                                                                                                  ggTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGt                                >  1:948010/1‑148 (MQ=255)

                                                                                                                  ggTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGt                                <  2:3027475/148‑1 (MQ=255)

                                                                                                                   gtgtTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTa                               <  2:841690/148‑1 (MQ=255)

                                                                                                                    tgtTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTaa                              <  1:2576476/148‑1 (MQ=255)

                                                                                                                     gtTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAAc                             >  2:235309/1‑148 (MQ=255)

                                                                                                                      ttAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACt                            >  2:2312729/1‑148 (MQ=255)

                                                                                                                      ttAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACt                            >  1:3114317/1‑148 (MQ=255)

                                                                                                                       tAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTc                           <  1:2120133/148‑1 (MQ=255)

                                                                                                                        aGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCa                          <  1:655733/148‑1 (MQ=255)

                                                                                                                        aGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCa                          <  2:3444753/148‑1 (MQ=255)

                                                                                                                          gCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAATGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATc                        <  1:613430/148‑1 (MQ=255)

                                                                                                                             aCATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTc                     <  1:1556648/148‑1 (MQ=255)

                                                                                                                              cATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCa                    <  2:751665/148‑1 (MQ=255)

                                                                                                                               aTGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAg                   <  2:625465/148‑1 (MQ=255)

                                                                                                                                 ggTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAgcg                 <  1:2679755/148‑1 (MQ=255)

                                                                                                                                 ggTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAgcg                 >  1:2256897/1‑148 (MQ=255)

                                                                                                                                 ggTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAgcg                 >  2:798588/1‑148 (MQ=255)

                                                                                                                                 ggTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAgcg                 >  2:3262863/1‑148 (MQ=255)

                                                                                                                                  gTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAgcgc                <  2:2901468/148‑1 (MQ=255)

                                                                                                                                   tCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCt               >  2:2182554/1‑148 (MQ=255)

                                                                                                                                   tCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCt               <  1:396411/148‑1 (MQ=255)

                                                                                                                                    cACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTa              <  2:3189772/148‑1 (MQ=255)

                                                                                                                                      cTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTTCCAACTCTTGTTAAAGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTAcc            >  1:3497750/1‑148 (MQ=255)

                                                                                                                                        aaCCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCACTTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGt          >  2:3175453/1‑148 (MQ=255)

                                                                                                                                          ccTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGa        >  1:503200/1‑148 (MQ=255)

                                                                                                                                             tATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGaaaa     <  2:528157/148‑1 (MQ=255)

                                                                                                                                               tCAACACAAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCTCCTTAAAGTCCGCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACATGTTATGGTAACTCATCGTCAGAGCTACCGTGAAAAAg   >  1:2798582/1‑148 (MQ=255)

                                                                                                                                                cAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGt  >  2:194518/1‑148 (MQ=255)

                                                                                                                                                cAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGt  <  1:436749/148‑1 (MQ=255)

ATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGT  >  CP009273/2277598‑2277889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`