Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,322,609 C→A 100% W223L (TGG→TTG)  yfaQ ← tandem DUF2300 domain protein, putative host defense protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,322,6090CA95.3% 604.3 / 18.0 203W223L (TGG→TTG) yfaQtandem DUF2300 domain protein, putative host defense protein
Reads supporting (aligned to +/- strand):  ref base C (3/7);  new base A (85/108);  total (88/115)
Fisher's exact test for biased strand distribution p-value = 5.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 203 total aligned reads displayed.

ATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCA  >  CP009273/2322468‑2322753
                                                                                                                                             |                                                                                                                                                
 ttttCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCgg                                                                                                                                           <  2:1319163/148‑1 (MQ=255)
     cttcAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTa                                                                                                                                       <  1:977458/148‑1 (MQ=255)
          gCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATc                                                                                                                                  <  2:316107/148‑1 (MQ=255)
          gCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATc                                                                                                                                  <  2:316054/148‑1 (MQ=255)
           cGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTCCCATCg                                                                                                                                 >  1:3307485/1‑148 (MQ=255)
              aTAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGtt                                                                                                                              >  1:958561/1‑148 (MQ=255)
               tAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCACATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTa                                                                                                                             >  2:1158738/1‑148 (MQ=255)
                 gCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAAc                                                                                                                           >  1:2986500/1‑148 (MQ=255)
                 gCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAAc                                                                                                                           <  2:2306386/148‑1 (MQ=255)
                 gCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAAc                                                                                                                           <  2:2307259/148‑1 (MQ=255)
                  cGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAAcc                                                                                                                          <  2:1040594/148‑1 (MQ=255)
                    ggCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAAccag                                                                                                                        <  2:342002/148‑1 (MQ=255)
                          cAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCGGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCa                                                                                                                  >  1:3326408/1‑148 (MQ=255)
                          cAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCa                                                                                                                  >  2:1604227/1‑148 (MQ=255)
                          cAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCa                                                                                                                  >  1:1558408/1‑148 (MQ=255)
                            gCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCaaa                                                                                                                <  1:776763/148‑1 (MQ=255)
                                 aCCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAaccac                                                                                                           <  1:2024163/148‑1 (MQ=255)
                                 aCCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAaccac                                                                                                           <  1:2024402/148‑1 (MQ=255)
                                 aCCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAaccac                                                                                                           <  1:2166108/148‑1 (MQ=255)
                                  ccTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAaccacc                                                                                                          <  2:1111907/148‑1 (MQ=255)
                                  ccTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAaccacc                                                                                                          >  2:3358848/1‑148 (MQ=255)
                                     ctACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTg                                                                                                       <  2:1835708/148‑1 (MQ=255)
                                     ctACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTg                                                                                                       <  2:971327/148‑1 (MQ=255)
                                     cgacacATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTg                                                                                                       <  2:3163095/146‑1 (MQ=255)
                                       acacATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGAc                                                                                                     >  1:3101383/1‑148 (MQ=255)
                                        cacaTTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACg                                                                                                    >  1:1726314/1‑148 (MQ=255)
                                        cacaTTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACg                                                                                                    >  2:2298187/1‑148 (MQ=255)
                                         acaTTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACgg                                                                                                   <  2:689116/148‑1 (MQ=255)
                                         acaTTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACgg                                                                                                   <  2:2366381/148‑1 (MQ=255)
                                         acaTTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACgg                                                                                                   <  2:2819566/148‑1 (MQ=255)
                                          caTTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGa                                                                                                  <  2:1316420/148‑1 (MQ=255)
                                          caTTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGa                                                                                                  <  2:1316157/148‑1 (MQ=255)
                                          caTTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGa                                                                                                  >  1:931497/1‑148 (MQ=255)
                                           aTTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGAt                                                                                                 >  1:3232067/1‑148 (MQ=255)
                                             tGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGAttt                                                                                               >  1:1592086/1‑148 (MQ=255)
                                              gCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTc                                                                                              <  1:1006660/148‑1 (MQ=255)
                                               cccACTGGCAACCTGCGTCGGCACTGTCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTcc                                                                                             >  2:154067/1‑148 (MQ=255)
                                                  aCTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGt                                                                                          <  1:608799/148‑1 (MQ=255)
                                                      gCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTat                                                                                      <  2:2859398/148‑1 (MQ=255)
                                                       cAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTatc                                                                                     <  1:385220/148‑1 (MQ=255)
                                                         aCCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTTCCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTatcat                                                                                   <  2:334393/148‑1 (MQ=255)
                                                              cGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCa                                                                              >  2:1920089/1‑148 (MQ=255)
                                                              cGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCAGACGGATTTCCTGTTTATCATCGGCa                                                                              >  2:120171/1‑148 (MQ=255)
                                                                 cGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAAc                                                                           >  1:469956/1‑148 (MQ=255)
                                                                  ggCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAAcc                                                                          <  1:3509705/148‑1 (MQ=255)
                                                                   gCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCa                                                                         >  1:2265558/1‑148 (MQ=255)
                                                                   gCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCa                                                                         >  1:1972132/1‑148 (MQ=255)
                                                                       tGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTat                                                                                      <  1:3479279/131‑1 (MQ=255)
                                                                            aaaCCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGt                                                                <  1:3310953/148‑1 (MQ=255)
                                                                              aCCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGttt                                                              >  2:2182926/1‑148 (MQ=255)
                                                                              aCCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGttt                                                              >  2:3432844/1‑148 (MQ=255)
                                                                               cccGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGtttt                                                             >  2:1322384/1‑148 (MQ=255)
                                                                               cccGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGtttt                                                             <  2:210637/148‑1 (MQ=255)
                                                                               cccGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGtttt                                                             <  2:1057311/148‑1 (MQ=255)
                                                                                ccGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTc                                                            <  2:1994516/148‑1 (MQ=255)
                                                                                 cGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCa                                                           >  1:242856/1‑148 (MQ=255)
                                                                                   aTTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCAcc                                                         >  2:1798122/1‑148 (MQ=255)
                                                                                    ttAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCAccc                                                        >  1:1650785/1‑148 (MQ=255)
                                                                                       aGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTa                                                     >  2:1854635/1‑148 (MQ=255)
                                                                                       aGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTa                                                     >  1:2686304/1‑148 (MQ=255)
                                                                                           ccGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCg                                                 >  1:76708/1‑148 (MQ=255)
                                                                                              tCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACt                                              >  1:1942584/1‑148 (MQ=255)
                                                                                                  aTAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCg                                          <  2:3502415/148‑1 (MQ=255)
                                                                                                  aTAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCg                                          >  1:735659/1‑148 (MQ=255)
                                                                                                   tAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGa                                         >  1:540262/1‑148 (MQ=255)
                                                                                                    aaCGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAg                                        <  1:1244029/148‑1 (MQ=255)
                                                                                                    aaCGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAg                                        <  1:3017657/148‑1 (MQ=255)
                                                                                                      cGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCa                                      >  2:1006086/1‑148 (MQ=255)
                                                                                                      cGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCa                                      <  2:2348363/148‑1 (MQ=255)
                                                                                                        ggTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATg                                    >  2:154815/1‑148 (MQ=255)
                                                                                                         gTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATgg                                   <  1:3265256/148‑1 (MQ=255)
                                                                                                         gTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATgg                                   >  1:714985/1‑148 (MQ=255)
                                                                                                         gTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATgg                                   <  1:108932/148‑1 (MQ=255)
                                                                                                          ttAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGa                                  <  1:656593/148‑1 (MQ=255)
                                                                                                           tAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGAt                                 >  2:1872775/1‑148 (MQ=255)
                                                                                                             gTACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGc                               >  1:1275103/1‑148 (MQ=255)
                                                                                                              tACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCg                              >  2:729983/1‑148 (MQ=255)
                                                                                                              tACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCg                              >  2:945300/1‑148 (MQ=255)
                                                                                                              tACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCg                              >  1:2503322/1‑148 (MQ=255)
                                                                                                              tACCGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGAGTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCg                              <  1:23897/148‑1 (MQ=255)
                                                                                                                ccGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGac                            <  2:2330005/148‑1 (MQ=255)
                                                                                                                ccGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGac                            <  2:526915/148‑1 (MQ=255)
                                                                                                                 cGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGaca                           >  1:847259/1‑148 (MQ=255)
                                                                                                                 cGTTTTGCTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGaca                           >  2:1347753/1‑148 (MQ=255)
                                                                                                                    tttGCTGGTGCCACAGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCTCGCATGGATGCTACACCg                        >  2:295788/1‑148 (MQ=255)
                                                                                                                       gCTGGTGCCACTGCCAGTTACCAACAGCGGTGGAGCTTCGGTTAACCAGCCTGCAAATCCACCCTGTGGTATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGAtt                     <  1:2879735/148‑1 (MQ=255)
                                                                                                                        cTGGTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGAttt                    <  1:1891448/148‑1 (MQ=255)
                                                                                                                           gTGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCg                 >  2:3050988/1‑148 (MQ=255)
                                                                                                                            tGCCACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGc                <  1:1051889/148‑1 (MQ=255)
                                                                                                                              ccACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCt              <  1:3414749/148‑1 (MQ=255)
                                                                                                                              ccACTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCt              <  1:1805016/148‑1 (MQ=255)
                                                                                                                                 cTGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGt           >  2:389462/1‑148 (MQ=255)
                                                                                                                                  tGCCAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTc          <  1:2301527/148‑1 (MQ=255)
                                                                                                                                    ccAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCa        >  1:317677/1‑148 (MQ=255)
                                                                                                                                     cAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAg       >  2:2769997/1‑148 (MQ=255)
                                                                                                                                     cAGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAg       <  2:856288/148‑1 (MQ=255)
                                                                                                                                      aGTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAAGCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGc      >  2:3137772/1‑148 (MQ=255)
                                                                                                                                       gTAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGca     <  2:3307485/148‑1 (MQ=255)
                                                                                                                                        tAACCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGcac    >  1:1132231/1‑148 (MQ=255)
                                                                                                                                          aCCAACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGcacca  <  2:540262/148‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                
ATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCA  >  CP009273/2322468‑2322753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑