Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,342,897 C→A 100% D19Y (GAC→TAC)  inaA ← acid‑inducible Kdo/WaaP family putative kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,342,8970CA99.5% 691.1 / ‑4.3 214D19Y (GAC→TAC) inaAacid‑inducible Kdo/WaaP family putative kinase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (100/113);  total (101/113)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 214 total aligned reads displayed.

ACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTCGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAATGAATTT  >  CP009273/2342752‑2343041
                                                                                                                                                 |                                                                                                                                                
 cAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGcc                                                                                                                                               <  2:3100464/148‑1 (MQ=255)
   aTCGTTGGTCGTCCGAACGGATAAAGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCct                                                                                                                                             <  2:339298/148‑1 (MQ=255)
         ggTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTg                                                                                                                                       <  2:121523/148‑1 (MQ=255)
         ggTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTg                                                                                                                                       >  2:2685277/1‑148 (MQ=255)
           tCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGcc                                                                                                                                     <  2:3344512/148‑1 (MQ=255)
           tCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGcc                                                                                                                                     >  2:2008991/1‑148 (MQ=255)
              gCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCccac                                                                                                                                  >  2:411597/1‑148 (MQ=255)
                 gAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAg                                                                                                                               >  1:1485353/1‑148 (MQ=255)
                   aCGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTg                                                                                                                             <  1:1279214/148‑1 (MQ=255)
                   aCGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTg                                                                                                                             <  1:1280738/148‑1 (MQ=255)
                   aCGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTg                                                                                                                             >  1:1444062/1‑148 (MQ=255)
                    cGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGa                                                                                                                            <  1:2999967/148‑1 (MQ=255)
                    cGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGa                                                                                                                            <  2:1710724/148‑1 (MQ=255)
                        tAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTaa                                                                                                                        >  1:798935/1‑148 (MQ=255)
                        tAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTaa                                                                                                                        <  1:3336791/148‑1 (MQ=255)
                         aaCGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCAACAGTGATTaaa                                                                                                                       <  1:730982/148‑1 (MQ=255)
                             tACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATtcg                                                                                                                   >  2:2606380/1‑148 (MQ=255)
                             tACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATtcg                                                                                                                   >  1:1767727/1‑148 (MQ=255)
                              aCGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATtcgt                                                                                                                  >  1:1593072/1‑148 (MQ=255)
                              aCGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATtcgt                                                                                                                  >  1:1564736/1‑148 (MQ=255)
                                  aaTGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTa                                                                                                              <  2:347155/148‑1 (MQ=255)
                                  aaTGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTa                                                                                                              >  1:865628/1‑148 (MQ=255)
                                  aaTGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTa                                                                                                              <  1:1064436/148‑1 (MQ=255)
                                  aaTGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTa                                                                                                              >  1:1846934/1‑148 (MQ=255)
                                   aTGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTAc                                                                                                             >  1:197193/1‑148 (MQ=255)
                                   aTGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTAc                                                                                                             <  2:1582070/148‑1 (MQ=255)
                                   aTGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGAGTAAATTCGTCGTAc                                                                                                             >  2:1098286/1‑148 (MQ=255)
                                    tGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACt                                                                                                            <  1:634112/148‑1 (MQ=255)
                                       aaCAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTg                                                                                                         <  2:66180/148‑1 (MQ=255)
                                       aaCAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTg                                                                                                         <  2:66154/148‑1 (MQ=255)
                                        aCAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGc                                                                                                        <  1:1902626/148‑1 (MQ=255)
                                         cAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCt                                                                                                       >  1:1636753/1‑148 (MQ=255)
                                           gatgatGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGa                                                                                                     >  1:2331991/1‑148 (MQ=255)
                                            atgatgCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGaa                                                                                                    >  2:1128410/1‑148 (MQ=255)
                                            atgatgCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGaa                                                                                                    >  1:1639517/1‑148 (MQ=255)
                                             tgatgCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGaaa                                                                                                   >  1:677006/1‑148 (MQ=255)
                                                tgCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTg                                                                                                <  2:3486965/148‑1 (MQ=255)
                                                 gCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGc                                                                                               <  1:1937467/148‑1 (MQ=255)
                                                 gCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGAGATTAAATTCGTCGTACTTTGCTGAAACTGc                                                                                               >  1:2633869/1‑148 (MQ=255)
                                                           cTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTAt                                                                                     >  1:1213554/1‑148 (MQ=255)
                                                             ttACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCg                                                                                   >  1:3259691/1‑148 (MQ=255)
                                                               acatacaGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGcc                                                                                 >  2:1422988/1‑148 (MQ=255)
                                                                catacaGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCt                                                                                <  1:2408860/148‑1 (MQ=255)
                                                                catacaGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCt                                                                                <  2:1703853/148‑1 (MQ=255)
                                                                 atacaGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGATCATACCATTACGGCGATAGTTAGGCACTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTTCCATACACTATCGCCTg                                                                               >  2:1255570/1‑148 (MQ=255)
                                                                    caGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCa                                                                            <  1:1875719/148‑1 (MQ=255)
                                                                    caGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCa                                                                            >  1:2967847/1‑148 (MQ=255)
                                                                       cTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCAttt                                                                         >  2:598697/1‑148 (MQ=255)
                                                                          tttGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTtta                                                                      >  1:2283892/1‑148 (MQ=255)
                                                                            tGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTttatt                                                                    >  2:1191162/1‑148 (MQ=255)
                                                                              ccGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTaa                                                                  >  1:712220/1‑148 (MQ=255)
                                                                               cGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAAc                                                                 >  1:2232472/1‑148 (MQ=255)
                                                                                gTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACg                                                                <  2:3044759/148‑1 (MQ=255)
                                                                                gTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACg                                                                <  2:908720/148‑1 (MQ=255)
                                                                                 ttGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGa                                                               >  2:765601/1‑148 (MQ=255)
                                                                                 ttGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGa                                                               >  1:1032190/1‑148 (MQ=255)
                                                                                 ttGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGa                                                               >  2:2332784/1‑148 (MQ=255)
                                                                                 ttGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGa                                                               <  1:3230442/148‑1 (MQ=255)
                                                                                  tGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGAc                                                              >  1:3142495/1‑148 (MQ=255)
                                                                                    cgcTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACaa                                                            <  1:1626755/148‑1 (MQ=255)
                                                                                      cTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATg                                                          >  1:1231665/1‑148 (MQ=255)
                                                                                       tCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGa                                                         >  1:447608/1‑148 (MQ=255)
                                                                                         gACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACt                                                       <  2:1940379/148‑1 (MQ=255)
                                                                                           cGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACtat                                                     <  1:477721/148‑1 (MQ=255)
                                                                                                 gCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAggtgg                                               <  2:1767727/148‑1 (MQ=255)
                                                                                                  cACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAggtggt                                              <  1:212177/148‑1 (MQ=255)
                                                                                                    ccccGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTa                                            >  2:1921058/1‑148 (MQ=255)
                                                                                                     cccGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTAc                                           >  2:3172585/1‑148 (MQ=255)
                                                                                                     cccGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTAc                                           <  2:1661487/148‑1 (MQ=255)
                                                                                                     cccGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTAc                                           <  1:2722880/148‑1 (MQ=255)
                                                                                                     cccGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTCATTAAATTAGTCGTACTTTGATGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATAACAATAGGTGGTTAc                                           >  2:2271450/1‑148 (MQ=255)
                                                                                                      ccGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCAATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTAcc                                          >  2:2287359/1‑148 (MQ=255)
                                                                                                         cTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGa                                       <  1:2184573/148‑1 (MQ=255)
                                                                                                         cTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGa                                       <  1:1189963/148‑1 (MQ=255)
                                                                                                         cTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGa                                       <  1:392160/148‑1 (MQ=255)
                                                                                                          tCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAg                                      >  2:450961/1‑148 (MQ=255)
                                                                                                             tACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGaa                                   <  2:1000050/148‑1 (MQ=255)
                                                                                                              aCCATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGaaa                                  <  2:1636753/148‑1 (MQ=255)
                                                                                                               ccATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGaaaa                                 <  2:2832988/148‑1 (MQ=255)
                                                                                                                cATTACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAAt                                >  2:2419091/1‑148 (MQ=255)
                                                                                                                  ttACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCt                              <  2:1964625/148‑1 (MQ=255)
                                                                                                                   tACGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCtt                             <  1:343945/148‑1 (MQ=255)
                                                                                                                     cGGCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTaa                           >  2:835441/1‑148 (MQ=255)
                                                                                                                       gCGATAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATg                         <  2:3039631/148‑1 (MQ=255)
                                                                                                                          gtAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGaaa                      <  2:1487916/147‑1 (MQ=255)
                                                                                                                           tAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAAc                     >  1:3132781/1‑148 (MQ=255)
                                                                                                                           tAGTTAGGCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCAATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAAc                     <  1:2477109/148‑1 (MQ=255)
                                                                                                                                  gCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGt              >  1:2935822/1‑148 (MQ=255)
                                                                                                                                  gCTCTTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGt              <  2:2187899/148‑1 (MQ=255)
                                                                                                                                    tctTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTAt            >  1:1168841/1‑148 (MQ=255)
                                                                                                                                    tctTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTAt            <  2:3004596/148‑1 (MQ=255)
                                                                                                                                    tctTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTAt            <  1:2685277/148‑1 (MQ=255)
                                                                                                                                    tctTCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTAt            <  2:814667/148‑1 (MQ=255)
                                                                                                                                      ttCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTa          <  1:3057401/148‑1 (MQ=255)
                                                                                                                                      ttCAACCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTa          >  1:2522100/1‑148 (MQ=255)
                                                                                                                                      ttCAACCCAGTAGCCCTCTGTTGCACACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTAATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTa          <  2:3218599/148‑1 (MQ=255)
                                                                                                                                         aaCCCAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAATg       >  2:246368/1‑148 (MQ=255)
                                                                                                                                           cccAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTATTGaa     >  2:3320444/1‑148 (MQ=255)
                                                                                                                                           cccAGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAATGaa     >  2:2559370/1‑148 (MQ=255)
                                                                                                                                              aGTAGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAATGAAttt  >  2:505979/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCACCCCGCTCATACCATTACGGCGATAGTTAGGCTCTTCAACCCAGTCGCCCTCTGTTGCCCACCAGTGATTAAATTCGTCGTACTTTGCTGAAACTGCCATACCCTATCGCCTGTCATTTTTATTAACGACAATGACTATAGGTGGTTACCTGAGGAAAATCTTAATGAAACGTGTCGTATTAATGAATTT  >  CP009273/2342752‑2343041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑