Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,359,673 G→T 100% M89I (ATG→ATT)  arnB → uridine 5'‑(beta‑1‑threo‑pentapyranosyl‑4‑ulose diphosphate) aminotransferase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,359,6730GT99.5% 744.7 / ‑4.3 230M89I (ATG→ATT) arnBuridine 5'‑(beta‑1‑threo‑pentapyranosyl‑4‑ulose diphosphate) aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (139/89);  total (140/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 240 total aligned reads displayed.

CTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCCAGCAGATATT  >  CP009273/2359528‑2359817
                                                                                                                                                 |                                                                                                                                                
cTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAAtattat                                                                                                                                                >  2:1218491/1‑148 (MQ=255)
cTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAAtattat                                                                                                                                                >  2:812141/1‑148 (MQ=255)
  ttttGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTAttt                                                                                                                                              >  2:2222842/1‑148 (MQ=255)
  ttttGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTAttt                                                                                                                                              <  2:2943150/148‑1 (MQ=255)
    ttGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTcc                                                                                                                                            <  1:1177402/148‑1 (MQ=255)
       ccAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCttg                                                                                                                                         <  1:2752763/148‑1 (MQ=255)
       ccAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCttg                                                                                                                                         >  1:1222829/1‑148 (MQ=255)
        cAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCttgt                                                                                                                                        <  1:1015947/148‑1 (MQ=255)
         aGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACTCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCttgtt                                                                                                                                       >  1:2564818/1‑148 (MQ=255)
         aGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACTCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCttgtt                                                                                                                                       >  1:1313038/1‑148 (MQ=255)
          gTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCttgttg                                                                                                                                      >  1:3008396/1‑148 (MQ=255)
              aCGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTg                                                                                                                                  >  1:2336282/1‑148 (MQ=255)
                  gAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAAc                                                                                                                              >  2:3400125/1‑148 (MQ=255)
                     aTCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGcc                                                                                                                           >  1:1662392/1‑148 (MQ=255)
                     aTCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGcc                                                                                                                           <  1:362224/148‑1 (MQ=255)
                       cAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCgg                                                                                                                         >  2:1069817/1‑148 (MQ=255)
                             gCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAAtgg                                                                                                                   <  1:3339830/148‑1 (MQ=255)
                                aTCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAAtggtgg                                                                                                                <  1:3449716/148‑1 (MQ=255)
                                 tCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGa                                                                                                               >  1:3462594/1‑148 (MQ=255)
                                   gcgGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATg                                                                                                             <  1:2222694/148‑1 (MQ=255)
                                   gcgGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATg                                                                                                             <  1:3394738/148‑1 (MQ=255)
                                      gTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCg                                                                                                          <  2:910157/148‑1 (MQ=255)
                                       tCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGa                                                                                                         <  2:2802532/148‑1 (MQ=255)
                                        cAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGAc                                                                                                        >  2:1621150/1‑148 (MQ=255)
                                           ttCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTNCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACcgc                                                                                                     <  1:1807610/148‑1 (MQ=255)
                                           ttCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACcgc                                                                                                     >  2:733567/1‑148 (MQ=255)
                                           ttCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACcgc                                                                                                     >  2:2029848/1‑148 (MQ=255)
                                            tCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACcgcg                                                                                                    >  2:2249781/1‑148 (MQ=255)
                                            tCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACcgcg                                                                                                    >  2:26055/1‑148 (MQ=255)
                                            tCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACcgcg                                                                                                    <  1:3255433/148‑1 (MQ=255)
                                               gCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATa                                                                                                 <  2:2410968/148‑1 (MQ=255)
                                               gCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATa                                                                                                 >  1:1342457/1‑148 (MQ=255)
                                                     gccgGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGa                                                                                           <  1:812141/148‑1 (MQ=255)
                                                     gccgGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGa                                                                                           <  2:3005510/148‑1 (MQ=255)
                                                            tGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCAc                                                                                    >  2:3550135/1‑148 (MQ=255)
                                                             gCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACg                                                                                   >  2:1739935/1‑148 (MQ=255)
                                                              cATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGc                                                                                  <  1:3549807/148‑1 (MQ=255)
                                                                tatCACGCTAATTGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCt                                                                                >  1:2711120/1‑148 (MQ=255)
                                                                tatCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCt                                                                                >  1:2512465/1‑148 (MQ=255)
                                                                   cACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGaa                                                                             >  1:3162270/1‑148 (MQ=255)
                                                                    aCGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAg                                                                            >  2:683355/1‑148 (MQ=255)
                                                                        tAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTAt                                                                        <  1:2250890/148‑1 (MQ=255)
                                                                         aaTGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATc                                                                       >  1:1874024/1‑148 (MQ=255)
                                                                         aaTGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATc                                                                       >  2:1065945/1‑148 (MQ=255)
                                                                           tGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGa                                                                     >  2:3347559/1‑148 (MQ=255)
                                                                            ggCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAg                                                                    <  1:2222842/148‑1 (MQ=255)
                                                                            ggCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAg                                                                    >  2:168167/1‑148 (MQ=255)
                                                                             gCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGt                                                                   <  1:2636824/148‑1 (MQ=255)
                                                                              cGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTc                                                                  >  1:1274660/1‑148 (MQ=255)
                                                                                ttGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAg                                                                >  1:108887/1‑148 (MQ=255)
                                                                                    aaaTTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCAt                                                            <  2:2886106/148‑1 (MQ=255)
                                                                                    aaaTTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCAt                                                            <  2:2670251/148‑1 (MQ=255)
                                                                                    aaaTTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCAt                                                            <  2:3325233/148‑1 (MQ=255)
                                                                                     aaTTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCAtt                                                           <  2:1398548/148‑1 (MQ=255)
                                                                                     aaTTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCAtt                                                           >  1:1069160/1‑148 (MQ=255)
                                                                                       ttGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTAc                                                         >  2:1827649/1‑148 (MQ=255)
                                                                                       ttGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTAc                                                         >  1:515771/1‑148 (MQ=255)
                                                                                       ttGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTAc                                                         >  1:1234281/1‑148 (MQ=255)
                                                                                          gCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGcc                                                      >  2:2118043/1‑148 (MQ=255)
                                                                                              gggCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGc                                                  >  1:86876/1‑148 (MQ=255)
                                                                                               ggCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCa                                                 >  2:2702095/1‑148 (MQ=255)
                                                                                               ggCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCa                                                 >  1:1487712/1‑148 (MQ=255)
                                                                                               ggCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCa                                                 >  2:2982646/1‑148 (MQ=255)
                                                                                                  gATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTa                                              <  2:3471092/148‑1 (MQ=255)
                                                                                                   aTGAAGTGATTACGCCTTCCCTGACCTGGGTGTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTaa                                             >  2:966070/1‑148 (MQ=255)
                                                                                                    tGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTaaa                                            >  2:45770/1‑148 (MQ=255)
                                                                                                     gAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAg                                           <  2:1277874/148‑1 (MQ=255)
                                                                                                        gTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCca                                        <  1:291667/148‑1 (MQ=255)
                                                                                                            ttACGCCTTCCCTGACCTGGGTTTCAACCCTCAAGATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCcatcat                                    <  2:2005440/148‑1 (MQ=255)
                                                                                                             tACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCAtt                                   >  2:416511/1‑148 (MQ=255)
                                                                                                             tACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCAtt                                   >  1:2127521/1‑148 (MQ=255)
                                                                                                             tACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCAtt                                   <  1:2167963/148‑1 (MQ=255)
                                                                                                             tACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCAtt                                   >  2:2751133/1‑148 (MQ=255)
                                                                                                                gCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCg                                <  1:2023343/148‑1 (MQ=255)
                                                                                                                 ccTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCgg                               <  2:3427478/148‑1 (MQ=255)
                                                                                                                  cTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAATCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGt                              >  2:1677969/1‑148 (MQ=255)
                                                                                                                   ttCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTg                             >  1:1882728/1‑148 (MQ=255)
                                                                                                                    tCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGc                            <  2:1792815/148‑1 (MQ=255)
                                                                                                                     cccTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCa                           >  1:2881045/1‑148 (MQ=255)
                                                                                                                       cTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCAtt                         <  1:3347559/148‑1 (MQ=255)
                                                                                                                        tGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTa                        >  1:1769577/1‑148 (MQ=255)
                                                                                                                         gACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTAt                       >  2:2464992/1‑148 (MQ=255)
                                                                                                                          aCCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATg                      >  2:2797641/1‑148 (MQ=255)
                                                                                                                          aCCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATg                      <  1:2574249/148‑1 (MQ=255)
                                                                                                                            cTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGcc                    >  1:821503/1‑148 (MQ=255)
                                                                                                                             tGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCg                   >  2:3408159/1‑148 (MQ=255)
                                                                                                                             tGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCAGCATTCCGGTGCCTTCTGCCg                   >  2:2260910/1‑148 (MQ=255)
                                                                                                                               ggTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGt                 >  2:327677/1‑148 (MQ=255)
                                                                                                                                 tttCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTgc               >  2:365608/1‑148 (MQ=255)
                                                                                                                                   tCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTgcgc             >  1:1578872/1‑148 (MQ=255)
                                                                                                                                   tCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTgcgc             <  1:683355/148‑1 (MQ=255)
                                                                                                                                       cccTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCcagc         >  1:3253283/1‑148 (MQ=255)
                                                                                                                                         cTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCcagcag       >  2:350074/1‑148 (MQ=255)
                                                                                                                                          tCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCCAGCAGa      >  2:100125/1‑148 (MQ=255)
                                                                                                                                          tCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCCAGCAGa      >  2:104642/1‑148 (MQ=255)
                                                                                                                                           cAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCCAGCAGat     >  1:2362027/1‑148 (MQ=255)
                                                                                                                                            aaTATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCCAGCAGata    >  2:2903401/1‑148 (MQ=255)
                                                                                                                                             atatTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCCAGCAGatat   >  1:1154091/1‑148 (MQ=255)
                                                                                                                                              tattatTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCCAGCAGATAtt  <  2:2519454/148‑1 (MQ=255)
                                                                                                                                              tattatGTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCCAGCAGATAtt  <  2:1307797/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCCAGCAGATATT  >  CP009273/2359528‑2359817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑