Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,383,897 C→A 100% R363L (CGC→CTC)  nuoN ← NADH:ubiquinone oxidoreductase, membrane subunit N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,383,8970CA98.1% 478.7 / 1.1 155R363L (CGC→CTC) nuoNNADH:ubiquinone oxidoreductase, membrane subunit N
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (70/82);  total (72/83)
Fisher's exact test for biased strand distribution p-value = 5.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 155 total aligned reads displayed.

CACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGC  >  CP009273/2383752‑2384040
                                                                                                                                                 |                                                                                                                                               
caccaGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAgg                                                                                                                                               >  2:2961051/1‑148 (MQ=255)
caccaGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAgg                                                                                                                                               <  1:1753485/148‑1 (MQ=255)
 accaGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGt                                                                                                                                              <  2:2925116/148‑1 (MQ=255)
 accaGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGt                                                                                                                                              <  2:2769592/148‑1 (MQ=255)
 accaGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCATCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGt                                                                                                                                              >  1:877087/1‑148 (MQ=255)
  ccaGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTa                                                                                                                                             <  2:2256403/148‑1 (MQ=255)
  ccaGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTa                                                                                                                                             <  2:269885/148‑1 (MQ=255)
       caccacAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAgaga                                                                                                                                        <  2:3259398/148‑1 (MQ=255)
        accacAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGaa                                                                                                                                       <  1:487208/148‑1 (MQ=255)
        accacAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGaa                                                                                                                                       <  1:3493866/148‑1 (MQ=255)
        accacAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGaa                                                                                                                                       >  2:614377/1‑148 (MQ=255)
        accacAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGaa                                                                                                                                       >  2:611151/1‑148 (MQ=255)
        accacAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGaa                                                                                                                                       >  2:2049376/1‑148 (MQ=255)
         ccacAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGAAAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAAc                                                                                                                                      >  2:1895239/1‑148 (MQ=255)
          cacaAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACa                                                                                                                                     >  1:3562470/1‑148 (MQ=255)
          cacaAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACa                                                                                                                                     <  1:2242258/148‑1 (MQ=255)
             aaGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAggg                                                                                                                                  <  2:2749001/148‑1 (MQ=255)
              aGTTTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCCGGATACCGGCCAGAGACAGCATCAACACCGTCAACACTCCCGCCAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGa                                                                                                                                 >  1:1401156/1‑148 (MQ=255)
               gtgtGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGaa                                                                                                                                <  2:1362216/148‑1 (MQ=255)
                 gtgCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCTGAACAGACCGCGGTAAGAGAACAGGGAATc                                                                                                                              <  2:407858/148‑1 (MQ=255)
                 gtgCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATc                                                                                                                              <  1:2839404/148‑1 (MQ=255)
                     cTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCa                                                                                                                          <  1:3484829/148‑1 (MQ=255)
                        gACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATcc                                                                                                                       >  1:2682819/1‑148 (MQ=255)
                         acacCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCg                                                                                                                      >  1:2038625/1‑148 (MQ=255)
                          cacCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGACCAGGGAATCAGCATCCgg                                                                                                                     >  1:3115142/1‑148 (MQ=255)
                           accgaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCggg                                                                                                                    >  2:3396360/1‑148 (MQ=255)
                            ccgaccgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGc                                                                                                                   >  2:2640819/1‑148 (MQ=255)
                                 cgCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGa                                                                                                              <  2:129270/148‑1 (MQ=255)
                                    cAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATAc                                                                                                           <  1:408579/148‑1 (MQ=255)
                                        aCGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGc                                                                                                       <  2:2802621/148‑1 (MQ=255)
                                         cGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCt                                                                                                      >  1:2299838/1‑148 (MQ=255)
                                          gTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTg                                                                                                     <  1:876797/148‑1 (MQ=255)
                                               aCTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACAt                                                                                                <  1:2027923/148‑1 (MQ=255)
                                                cTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATc                                                                                               <  2:2518277/148‑1 (MQ=255)
                                                cTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATc                                                                                               <  2:2518356/148‑1 (MQ=255)
                                                cTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATc                                                                                               <  2:1772439/148‑1 (MQ=255)
                                                  tACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACTATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGTACATCAg                                                                                             >  1:2981615/1‑148 (MQ=255)
                                                   aCCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAgg                                                                                            <  2:102611/148‑1 (MQ=255)
                                                   aCCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAgg                                                                                            <  1:2412812/148‑1 (MQ=255)
                                                     cGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACAGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCt                                                                                          >  1:1051918/1‑148 (MQ=255)
                                                      gATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATc                                                                                               >  1:646985/1‑142 (MQ=255)
                                                       aTAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGa                                                                                        <  2:3126896/148‑1 (MQ=255)
                                                       aTAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGa                                                                                        <  2:3127603/148‑1 (MQ=255)
                                                        tAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGAc                                                                                       >  1:565866/1‑148 (MQ=255)
                                                         aaaGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGAcc                                                                                      >  1:1859693/1‑148 (MQ=255)
                                                           aGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCAc                                                                                    >  2:633007/1‑148 (MQ=255)
                                                            gCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACg                                                                                   >  2:3482029/1‑148 (MQ=255)
                                                               cAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCg                                                                                <  2:3014368/148‑1 (MQ=255)
                                                               cAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGACTGGACATCAGGCTGACCACGCCg                                                                                >  2:2277077/1‑148 (MQ=255)
                                                               cAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGACTGGACATCAGGCTGACCACGCCg                                                                                >  2:2277076/1‑148 (MQ=255)
                                                                aGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGa                                                                               >  2:2507765/1‑148 (MQ=255)
                                                                aGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGa                                                                               >  2:2387618/1‑148 (MQ=255)
                                                                 gCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGaa                                                                              <  2:2682819/148‑1 (MQ=255)
                                                                 gCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGAGGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGaa                                                                              <  2:3197468/148‑1 (MQ=255)
                                                                     cATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAAcgcg                                                                          >  2:1297122/1‑148 (MQ=255)
                                                                     cATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAAcgcg                                                                          >  2:1155825/1‑148 (MQ=255)
                                                                     cATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAAcgcg                                                                          <  1:2718234/148‑1 (MQ=255)
                                                                        cGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCg                                                                       <  2:1097114/148‑1 (MQ=255)
                                                                        cGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCg                                                                       <  2:1869519/148‑1 (MQ=255)
                                                                                      gagaCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAg                                                         <  2:1370450/148‑1 (MQ=255)
                                                                                      gagaCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAg                                                         >  2:2134951/1‑148 (MQ=255)
                                                                                       agaCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGa                                                        <  1:3202149/148‑1 (MQ=255)
                                                                                       agaCAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGa                                                        >  2:3205855/1‑148 (MQ=255)
                                                                                          cAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATaa                                                     <  2:1406172/148‑1 (MQ=255)
                                                                                          cAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATaa                                                     <  1:2961051/148‑1 (MQ=255)
                                                                                          cAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATaa                                                     <  1:2640819/148‑1 (MQ=255)
                                                                                          aatcataatCACCGTCATCACTGCAGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTTACCACGCCGAACGCGCCGAGGCTGCTGAACAGATaa                                                     <  2:565860/145‑1 (MQ=255)
                                                                                            gCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAAcc                                                   >  2:3293805/1‑148 (MQ=255)
                                                                                              atcatcACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCAGAGGCTGCTGAACAGATAACCgg                                                 >  1:2861418/1‑148 (MQ=255)
                                                                                               tcatcaCCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGc                                                <  2:1251997/148‑1 (MQ=255)
                                                                                               tcatcaCCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGc                                                <  1:566860/148‑1 (MQ=255)
                                                                                               tcatcaCCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGc                                                >  1:636549/1‑148 (MQ=255)
                                                                                                 atcaCCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCa                                              <  1:2512533/148‑1 (MQ=255)
                                                                                                    aCCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGt                                           <  1:2385812/148‑1 (MQ=255)
                                                                                                     ccGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGTCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa                                          >  1:455402/1‑148 (MQ=255)
                                                                                                         catcaCTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAAcc                                      >  2:1018634/1‑148 (MQ=255)
                                                                                                         catcaCTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAAcc                                      >  2:1478371/1‑148 (MQ=255)
                                                                                                         catcaCTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAAcc                                      >  2:2796803/1‑148 (MQ=255)
                                                                                                          atcaCTGCCGCGAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGATGCTGAACAGATAACCGGCCAGGTAAAccc                                     >  1:1915357/1‑148 (MQ=255)
                                                                                                               tGCCGCGAGGATCGGACGATGCCTGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTAcc                                >  2:3481830/1‑148 (MQ=255)
                                                                                                                    cgAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTc                           >  2:1707490/1‑148 (MQ=255)
                                                                                                                    cgAGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTc                           >  1:1522369/1‑148 (MQ=255)
                                                                                                                      aGGATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCa                         <  1:3396360/148‑1 (MQ=255)
                                                                                                                       ggATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCAt                        <  1:1155836/148‑1 (MQ=255)
                                                                                                                       ggATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCAt                        <  1:1297122/148‑1 (MQ=255)
                                                                                                                       ggATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCAt                        <  1:1155825/148‑1 (MQ=255)
                                                                                                                       ggATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCAt                        <  2:877087/148‑1 (MQ=255)
                                                                                                                        gATCGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATc                       >  1:1963640/1‑148 (MQ=255)
                                                                                                                           cGGACGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGAc                    <  1:3272557/148‑1 (MQ=255)
                                                                                                                              aCGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACAtc                 >  2:3299974/1‑148 (MQ=255)
                                                                                                                              aCGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACAtc                 >  2:3299982/1‑148 (MQ=255)
                                                                                                                              aCGATGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACAtc                 >  2:2091337/1‑148 (MQ=255)
                                                                                                                                 aTGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCg              >  1:3099345/1‑148 (MQ=255)
                                                                                                                                  tGCCAGAACAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGc             <  1:3482029/148‑1 (MQ=255)
                                                                                                                                        aaCAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGttt       >  2:2195781/1‑148 (MQ=255)
                                                                                                                                          cAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGc     <  1:2049376/148‑1 (MQ=255)
                                                                                                                                          cAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGc     <  1:2796803/148‑1 (MQ=255)
                                                                                                                                          cAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGc     >  1:1345027/1‑148 (MQ=255)
                                                                                                                                          cAGACCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGc     <  1:1018634/148‑1 (MQ=255)
                                                                                                                                             aCCGAGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAgc  >  2:488219/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGACAGCATCATCACCGTCATCACTGCCGCGAGGATCGGACGATGCCAGAACAGACCGCGGTAAGAGAACAGGGAATCAGCATCCGGGCCACGATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGC  >  CP009273/2383752‑2384040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑