Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 217,932 A→C 100% I59M (ATT→ATG)  metI ← DL‑methionine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273217,9320AC97.3% 762.5 / 1.4 194I59M (ATT→ATG) metIDL‑methionine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (1/4);  new base C (106/82);  total (107/87)
Fisher's exact test for biased strand distribution p-value = 1.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 196 total aligned reads displayed.

CCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATACGCCA  >  CP009273/217787‑218076
                                                                                                                                                 |                                                                                                                                                
        gCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGaaa                                                                                                                                        >  2:873594/1‑148 (MQ=255)
         cAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAAc                                                                                                                                       <  2:1160201/148‑1 (MQ=255)
           aTAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACgg                                                                                                                                     >  2:3084283/1‑148 (MQ=255)
             aaaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTa                                                                                                                                   >  2:3132657/1‑148 (MQ=255)
              aaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTAc                                                                                                                                  >  2:2879649/1‑148 (MQ=255)
               agggTGCTTGACCAAGGTTTAACGGAAGAATCGCTGCCTGCACACCAATCGCTGTACCGACATTAACGCGGGTTAACGGAATCATACATACAAGCAAGATAATGCACGGGATGGAACGGAAAATGTTCACTATCGCAGAAACGGTACg                                                                                                                                 <  2:429306/146‑1 (MQ=255)
               aCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACg                                                                                                                                 >  2:262447/1‑148 (MQ=255)
               aCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACg                                                                                                                                 <  2:2045118/148‑1 (MQ=255)
               aCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACg                                                                                                                                 >  1:1541147/1‑148 (MQ=255)
               aCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACg                                                                                                                                 <  1:651264/148‑1 (MQ=255)
                cGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGa                                                                                                                                >  1:510311/1‑148 (MQ=255)
                  gTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATa                                                                                                                              <  1:2467743/148‑1 (MQ=255)
                  gTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATa                                                                                                                              >  2:1412360/1‑148 (MQ=255)
                  gTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATa                                                                                                                              <  1:1165259/148‑1 (MQ=255)
                   tgctgcACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATAc                                                                                                                             <  2:1981159/148‑1 (MQ=255)
                    gctgcACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACa                                                                                                                            <  1:1725193/148‑1 (MQ=255)
                       gcACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCt                                                                                                                         <  2:1148069/148‑1 (MQ=255)
                           cAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTcgc                                                                                                                     >  1:1219939/1‑148 (MQ=255)
                            aaCGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTcgcg                                                                                                                    <  1:982591/148‑1 (MQ=255)
                            aaCGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTcgcg                                                                                                                    <  1:3262147/148‑1 (MQ=255)
                             aCGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGt                                                                                                                   <  2:1823573/148‑1 (MQ=255)
                                  tAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCa                                                                                                              <  2:849825/148‑1 (MQ=255)
                                    aCGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaat                                                                                                            <  2:1187777/148‑1 (MQ=255)
                                     cGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaata                                                                                                           <  2:940210/148‑1 (MQ=255)
                                     cGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaata                                                                                                           >  2:1008760/1‑148 (MQ=255)
                                       gAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaataat                                                                                                         >  2:912139/1‑148 (MQ=255)
                                        aaCAATCGCTGCCTTCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAAtt                                                                                                        <  2:1861037/148‑1 (MQ=255)
                                        aaCAATCGCTGCCTGCAAACCAATCGATGTACCGACAATACCGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAAtt                                                                                                        <  2:2767545/148‑1 (MQ=255)
                                         aCAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGTAACGGTACGATACAGCTTCGCGTTAGCAATAAttt                                                                                                       >  2:250304/1‑148 (MQ=255)
                                          cAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTCGCAATAATTTg                                                                                                      >  1:453126/1‑148 (MQ=255)
                                          cAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTg                                                                                                      <  2:1782281/148‑1 (MQ=255)
                                          cAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTg                                                                                                      >  1:536642/1‑148 (MQ=255)
                                          cAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTg                                                                                                      >  1:1547712/1‑148 (MQ=255)
                                               gCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATTGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCg                                                                                                 <  2:3319667/148‑1 (MQ=255)
                                                      gcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGt                                                                                          <  1:11018/148‑1 (MQ=255)
                                                      gcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGt                                                                                          >  2:3090365/1‑148 (MQ=255)
                                                      gcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGt                                                                                          <  1:1932349/148‑1 (MQ=255)
                                                      gcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGt                                                                                          <  1:1248496/148‑1 (MQ=255)
                                                          aCCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACa                                                                                      <  2:1219939/148‑1 (MQ=255)
                                                             aaTCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATaa                                                                                   >  2:2244633/1‑148 (MQ=255)
                                                             aaTCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATaa                                                                                   <  1:2096133/148‑1 (MQ=255)
                                                               tCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAg                                                                                 <  2:1257004/148‑1 (MQ=255)
                                                                cGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGc                                                                                <  1:3079490/148‑1 (MQ=255)
                                                                cGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGc                                                                                <  2:483692/148‑1 (MQ=255)
                                                                cGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGc                                                                                <  1:2510045/148‑1 (MQ=255)
                                                                  aTGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAg                                                                              >  1:2618884/1‑148 (MQ=255)
                                                                          aCAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCaa                                                                      >  1:271893/1‑148 (MQ=255)
                                                                          aCAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCaa                                                                      >  2:1080628/1‑148 (MQ=255)
                                                                           cAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAAc                                                                     <  2:196122/148‑1 (MQ=255)
                                                                              tAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCgg                                                                  <  1:1194276/148‑1 (MQ=255)
                                                                              tAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCgg                                                                  >  1:1826875/1‑148 (MQ=255)
                                                                               aaCGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGc                                                                 <  1:2044164/148‑1 (MQ=255)
                                                                                aCGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCa                                                                >  1:865655/1‑148 (MQ=255)
                                                                                      gTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCaa                                                          <  1:2338501/148‑1 (MQ=255)
                                                                                      gTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCaa                                                          >  2:497313/1‑148 (MQ=255)
                                                                                       tAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAAt                                                         >  1:2521438/1‑148 (MQ=255)
                                                                                       tAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAAt                                                         >  1:2521412/1‑148 (MQ=255)
                                                                                        aaaCGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATc                                                        <  1:3551073/148‑1 (MQ=255)
                                                                                             gAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACaaa                                                   >  2:2967529/1‑148 (MQ=255)
                                                                                             gAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACaaa                                                   >  2:2989836/1‑148 (MQ=255)
                                                                                               atcattCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCACTAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGc                                                 >  2:3429061/1‑148 (MQ=255)
                                                                                               atcattCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAATGCAGAACGCCAACCGGCAGACCAATCACAAAGc                                                 <  2:3261764/148‑1 (MQ=255)
                                                                                               atcattCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGc                                                 >  1:2655143/1‑148 (MQ=255)
                                                                                                    tcATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCaaaa                                            >  1:3378679/2‑148 (MQ=255)
                                                                                                      aTACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCaacaaa                                          >  2:3183152/1‑148 (MQ=255)
                                                                                                      aTACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCaaaaaa                                          >  1:436269/1‑148 (MQ=255)
                                                                                                      aTACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCaaaaaa                                          <  2:2205476/148‑1 (MQ=255)
                                                                                                      aTACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCaaaaaa                                          >  2:1878201/1‑148 (MQ=255)
                                                                                                       tACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCaaaaaaa                                         >  1:17671/1‑148 (MQ=255)
                                                                                                       tACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCaaaaaaa                                         >  1:670450/1‑148 (MQ=255)
                                                                                                           agcaagATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCgg                                     <  2:2143451/148‑1 (MQ=255)
                                                                                                             caagATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGAt                                   <  2:1596292/148‑1 (MQ=255)
                                                                                                             caagATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGAt                                   >  1:1070099/1‑148 (MQ=255)
                                                                                                               agATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATAc                                 >  2:524533/1‑148 (MQ=255)
                                                                                                                 aTAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGa                               <  1:1608273/148‑1 (MQ=255)
                                                                                                                 aTAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGa                               <  1:2550700/148‑1 (MQ=255)
                                                                                                                 aTAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGa                               <  2:2815198/148‑1 (MQ=255)
                                                                                                                   aaTGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAg                             <  2:2553110/148‑1 (MQ=255)
                                                                                                                   aaTGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAg                             <  2:766218/148‑1 (MQ=255)
                                                                                                                    aTGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAgg                            >  1:493847/1‑148 (MQ=255)
                                                                                                                     tGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGt                           <  2:1226169/148‑1 (MQ=255)
                                                                                                                     tGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGt                           >  1:1156551/1‑148 (MQ=255)
                                                                                                                     tGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGt                           >  2:1008854/1‑148 (MQ=255)
                                                                                                                         cGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCAtt                       >  2:2648619/1‑148 (MQ=255)
                                                                                                                               ggAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGc                 <  1:1361437/148‑1 (MQ=255)
                                                                                                                                  aCGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGtt              <  1:2446462/148‑1 (MQ=255)
                                                                                                                                  aCGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGtt              >  1:1161762/1‑148 (MQ=255)
                                                                                                                                   cGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGttt             <  1:3417206/148‑1 (MQ=255)
                                                                                                                                   cGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGttt             >  2:3314591/1‑148 (MQ=255)
                                                                                                                                        aaTGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCAt        <  2:1705463/148‑1 (MQ=255)
                                                                                                                                        aaTGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCAt        >  2:1360377/1‑148 (MQ=255)
                                                                                                                                         aTGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATa       <  1:1505491/148‑1 (MQ=255)
                                                                                                                                         aTGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATa       <  2:1566738/148‑1 (MQ=255)
                                                                                                                                          tGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATAc      >  1:3179307/1‑148 (MQ=255)
                                                                                                                                          tGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATAc      >  1:742965/1‑148 (MQ=255)
                                                                                                                                           gTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATACg     >  1:2840817/1‑148 (MQ=255)
                                                                                                                                             tCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATACGcc   <  1:1443346/148‑1 (MQ=255)
                                                                                                                                             tCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATACGcc   >  1:2954586/1‑148 (MQ=255)
                                                                                                                                              caccATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATACGCCa  >  1:2932353/1‑148 (MQ=255)
                                                                                                                                              caccATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATACGCCa  >  1:1370194/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATACGCCA  >  CP009273/217787‑218076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑