Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,422,841 C→A 100% D293Y (GAT→TAT)  purF ← amidophosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,422,8410CA99.4% 548.4 / ‑4.2 172D293Y (GAT→TAT) purFamidophosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (83/88);  total (83/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 172 total aligned reads displayed.

GCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGC  >  CP009273/2422697‑2422985
                                                                                                                                                |                                                                                                                                                
gctgGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATGACCACGTCGATATCCAGATATTc                                                                                                                                               <  2:224217/148‑1 (MQ=255)
gctgGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTc                                                                                                                                               <  2:1983478/148‑1 (MQ=255)
  tgGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTccc                                                                                                                                             <  1:2568058/148‑1 (MQ=255)
  tgGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTccc                                                                                                                                             <  1:450302/148‑1 (MQ=255)
  tgGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTccc                                                                                                                                             <  1:2115857/148‑1 (MQ=255)
   ggCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCa                                                                                                                                            <  1:1918098/148‑1 (MQ=255)
    gCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCAt                                                                                                                                           >  1:2993597/1‑148 (MQ=255)
    gCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCAt                                                                                                                                           >  2:2449459/1‑148 (MQ=255)
     cccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCAtt                                                                                                                                          >  1:1730646/1‑148 (MQ=255)
             gATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCa                                                                                                                                  >  2:787311/1‑148 (MQ=255)
                aaaGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAAtt                                                                                                                               >  2:3153912/1‑148 (MQ=255)
                aaaGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAAtt                                                                                                                               <  1:973189/148‑1 (MQ=255)
                aaaGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAAtt                                                                                                                               >  1:3147105/1‑148 (MQ=255)
                aaaGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAAtt                                                                                                                               >  2:348143/1‑148 (MQ=255)
                aaaGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAAtt                                                                                                                               >  1:753217/1‑148 (MQ=255)
                aaaGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAAtt                                                                                                                               >  1:753128/1‑148 (MQ=255)
                 aaGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAAttt                                                                                                                              <  1:2308101/148‑1 (MQ=255)
                    gTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTtct                                                                                                                           <  2:3330374/148‑1 (MQ=255)
                    gTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTtct                                                                                                                           <  1:1304447/148‑1 (MQ=255)
                        ggCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGcc                                                                                                                       <  2:625710/148‑1 (MQ=255)
                          ccAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCa                                                                                                                     >  1:3564338/1‑148 (MQ=255)
                            aaCATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGt                                                                                                                   <  1:207159/148‑1 (MQ=255)
                            aaCATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGt                                                                                                                   >  2:471868/1‑148 (MQ=255)
                             aCATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGtt                                                                                                                  >  1:790829/1‑148 (MQ=255)
                             aCATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGtt                                                                                                                  >  1:3413031/1‑148 (MQ=255)
                              cATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGttt                                                                                                                 >  2:49347/1‑148 (MQ=255)
                              cATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGttt                                                                                                                 >  2:2352687/1‑148 (MQ=255)
                                 aGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGt                                                                                                              >  2:1272821/1‑148 (MQ=255)
                                 aGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGt                                                                                                              >  2:969641/1‑148 (MQ=255)
                                   cGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGc                                                                                                            >  2:1605954/1‑148 (MQ=255)
                                   cGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGc                                                                                                            >  2:1606121/1‑148 (MQ=255)
                                   cGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGc                                                                                                            >  2:3375269/1‑148 (MQ=255)
                                    ggTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGcc                                                                                                           <  1:2873864/148‑1 (MQ=255)
                                    ggTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGcc                                                                                                           <  1:2873906/148‑1 (MQ=255)
                                     gTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGccc                                                                                                          <  2:2471939/148‑1 (MQ=255)
                                     gTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGccc                                                                                                          <  1:3146888/148‑1 (MQ=255)
                                     gTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGccc                                                                                                          <  1:3146941/148‑1 (MQ=255)
                                             cGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTcac                                                                                                  >  2:1933440/1‑148 (MQ=255)
                                             cGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTcac                                                                                                  <  1:1012407/148‑1 (MQ=255)
                                              gAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTcaca                                                                                                 <  1:1432560/148‑1 (MQ=255)
                                               aaGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCacac                                                                                                >  2:857004/1‑148 (MQ=255)
                                                    cTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACAcgcgcg                                                                                           >  2:2797927/1‑148 (MQ=255)
                                                    cTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACAcgcgcg                                                                                           <  2:1268588/148‑1 (MQ=255)
                                                       gCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACCCGCGCGCTg                                                                                        >  1:3352018/1‑148 (MQ=255)
                                                       gCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTg                                                                                        <  1:1302512/148‑1 (MQ=255)
                                                           tACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTaaa                                                                                    <  1:730770/148‑1 (MQ=255)
                                                           tACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTaaa                                                                                    >  1:2697289/1‑148 (MQ=255)
                                                           tACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTaaa                                                                                    >  2:434671/1‑148 (MQ=255)
                                                              ggTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACgg                                                                                 >  2:1901250/1‑148 (MQ=255)
                                                              ggTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACgg                                                                                 <  2:3217946/148‑1 (MQ=255)
                                                               gTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGa                                                                                >  2:1277337/1‑148 (MQ=255)
                                                                tttGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGAAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGaa                                                                               <  1:2539396/148‑1 (MQ=255)
                                                                        gAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTc                                                                       >  2:515464/1‑148 (MQ=255)
                                                                        gAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTc                                                                       >  2:516080/1‑148 (MQ=255)
                                                                           tACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGAt                                                                    >  2:2977735/1‑148 (MQ=255)
                                                                             cGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATaa                                                                  <  1:3269518/148‑1 (MQ=255)
                                                                                   aTTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAACTTTTGTCGATAAACGAGt                                                            >  2:643518/1‑148 (MQ=255)
                                                                                   aTTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGt                                                            >  1:1612132/1‑148 (MQ=255)
                                                                                   aTTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGt                                                            <  2:1498067/148‑1 (MQ=255)
                                                                                    tttCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTc                                                           >  2:503840/1‑148 (MQ=255)
                                                                                     ttCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTcc                                                          >  1:1501063/1‑148 (MQ=255)
                                                                                       ccAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcgg                                                        <  2:2677621/148‑1 (MQ=255)
                                                                                          gcgcGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcgggcg                                                     >  1:1459244/1‑148 (MQ=255)
                                                                                          gcgcGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcgggcg                                                     >  2:3414762/1‑148 (MQ=255)
                                                                                          gcgcGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcgggcg                                                     >  2:1033642/1‑148 (MQ=255)
                                                                                                 atCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAg                                              >  1:2576844/1‑148 (MQ=255)
                                                                                                  tCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGt                                             <  1:396095/148‑1 (MQ=255)
                                                                                                     cacGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGtata                                          <  1:1552352/148‑1 (MQ=255)
                                                                                                            tttCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTc                                   <  2:1367188/148‑1 (MQ=255)
                                                                                                              tCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCaa                                 <  2:1920248/148‑1 (MQ=255)
                                                                                                              tCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCaa                                 <  2:136386/148‑1 (MQ=255)
                                                                                                                tGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAAc                               <  2:2993597/148‑1 (MQ=255)
                                                                                                                tGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAAc                               <  1:785259/148‑1 (MQ=255)
                                                                                                                 gggatcgggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACa                              >  1:141075/1‑148 (MQ=255)
                                                                                                                 gggatcgggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACa                              <  1:1157977/148‑1 (MQ=255)
                                                                                                                 gggatcgggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACa                              >  1:1941248/1‑148 (MQ=255)
                                                                                                                 gggatcgggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACa                              <  1:2936021/148‑1 (MQ=255)
                                                                                                                  ggatcgggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAg                             >  1:1261152/1‑148 (MQ=255)
                                                                                                                  ggatcgggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAg                             >  1:3036949/1‑148 (MQ=255)
                                                                                                                   gatcgggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAgg                            >  2:1974401/1‑148 (MQ=255)
                                                                                                                   gatcgggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAgg                            >  2:172618/1‑148 (MQ=255)
                                                                                                                    atcgggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGc                           <  2:1982339/148‑1 (MQ=255)
                                                                                                                    atcgggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGc                           <  2:1247869/148‑1 (MQ=255)
                                                                                                                        ggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACgg                       <  1:1901250/148‑1 (MQ=255)
                                                                                                                        ggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACgg                       <  1:2357538/148‑1 (MQ=255)
                                                                                                                        ggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACgg                       <  1:2357544/148‑1 (MQ=255)
                                                                                                                        ggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACgg                       <  2:790772/148‑1 (MQ=255)
                                                                                                                          atcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGAt                     >  2:2294409/1‑148 (MQ=255)
                                                                                                                              ccacGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCt                 >  1:1657375/1‑148 (MQ=255)
                                                                                                                               cacGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCACAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTGGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTg                <  2:727009/148‑1 (MQ=255)
                                                                                                                                    cGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGGAAAGTATACATACTCAAACAGGCACGGATTGCTGACCgg           >  2:2220317/1‑148 (MQ=255)
                                                                                                                                      atatCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGAt         <  1:1042126/148‑1 (MQ=255)
                                                                                                                                        atCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTg       <  1:938626/148‑1 (MQ=255)
                                                                                                                                        atCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTg       >  1:3396579/1‑148 (MQ=255)
                                                                                                                                         tCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGt      >  1:314251/1‑148 (MQ=255)
                                                                                                                                         tCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGt      >  1:2460923/1‑148 (MQ=255)
                                                                                                                                          ccAGATAGTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTc     <  2:2253406/148‑1 (MQ=255)
                                                                                                                                            aGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAg   >  1:2170349/1‑148 (MQ=255)
                                                                                                                                             gATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGc  >  2:657432/1‑148 (MQ=255)
                                                                                                                                             gATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGc  >  1:259699/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGC  >  CP009273/2422697‑2422985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑