Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,481,974 G→T 100% S225* (TCA→TAA)  yfdE ← acetyl‑CoA:oxalate CoA‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,481,9740GT97.4% 739.2 / 8.6 235S225* (TCA→TAA) yfdEacetyl‑CoA:oxalate CoA‑transferase
Reads supporting (aligned to +/- strand):  ref base G (2/4);  new base T (133/96);  total (135/100)
Fisher's exact test for biased strand distribution p-value = 4.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 240 total aligned reads displayed.

TTAACCAGTTCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACAC  >  CP009273/2481830‑2482118
                                                                                                                                                |                                                                                                                                                
ttAACCAGTTCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCAGTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACt                                                                                                                                               <  2:2506106/148‑1 (MQ=255)
 tAACCAGTTCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACtt                                                                                                                                              <  2:281940/148‑1 (MQ=255)
   aCCAGTTCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTcc                                                                                                                                            >  1:379887/1‑148 (MQ=255)
    ccAGTTCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTccc                                                                                                                                           >  2:501369/1‑148 (MQ=255)
    ccAGTTCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTccc                                                                                                                                           >  2:3498810/1‑148 (MQ=255)
        ttCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGtt                                                                                                                                       >  1:3274848/1‑148 (MQ=255)
         tCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCAGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCAGGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTg                                                                                                                                      >  2:2428128/1‑148 (MQ=255)
          ccGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGc                                                                                                                                     <  1:746765/148‑1 (MQ=255)
            gTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGa                                                                                                                                   <  1:1027040/148‑1 (MQ=255)
              aaGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGata                                                                                                                                 >  1:1302576/1‑148 (MQ=255)
                gCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGatata                                                                                                                               <  2:723280/148‑1 (MQ=255)
                  tCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATg                                                                                                                             >  1:1615450/1‑148 (MQ=255)
                    cAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGcc                                                                                                                           >  2:1879954/1‑148 (MQ=255)
                      gTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGTCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCAt                                                                                                                         >  2:889105/1‑148 (MQ=255)
                       tGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATc                                                                                                                        >  1:3176578/1‑148 (MQ=255)
                       tGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATc                                                                                                                        >  2:24421/1‑148 (MQ=255)
                         ccTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAg                                                                                                                      <  1:454687/148‑1 (MQ=255)
                         catGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAg                                                                                                                      <  1:2577623/146‑1 (MQ=255)
                          cTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGa                                                                                                                     <  1:2971757/148‑1 (MQ=255)
                          cTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGa                                                                                                                     <  2:773735/148‑1 (MQ=255)
                           tGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGAc                                                                                                                    <  2:262424/148‑1 (MQ=255)
                           tGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGAc                                                                                                                    >  1:2001373/1‑148 (MQ=255)
                            ggCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGAcc                                                                                                                   <  2:916102/148‑1 (MQ=255)
                             gCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCa                                                                                                                  >  2:1743470/1‑148 (MQ=255)
                                tAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGc                                                                                                               >  2:2133295/1‑148 (MQ=255)
                                tAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGc                                                                                                               >  1:873839/1‑148 (MQ=255)
                                 aaCGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCt                                                                                                              >  2:2808247/1‑148 (MQ=255)
                                 aaCGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCt                                                                                                              >  2:3036708/1‑148 (MQ=255)
                                  aCGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTc                                                                                                             <  2:1131816/148‑1 (MQ=255)
                                        aaaaaaGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGaaa                                                                                                       >  1:3496371/1‑148 (MQ=255)
                                         aaaaaGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGaaaa                                                                                                      <  2:2972394/148‑1 (MQ=255)
                                          aaaaGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAAc                                                                                                     >  1:2297205/1‑148 (MQ=255)
                                          aaaaGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAAc                                                                                                     >  1:504231/1‑148 (MQ=255)
                                          aaaaGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAAc                                                                                                     >  2:816357/1‑148 (MQ=255)
                                           aaaGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACt                                                                                                    <  2:2896446/148‑1 (MQ=255)
                                           aaaGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACt                                                                                                    <  2:1118171/148‑1 (MQ=255)
                                           aaaGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACt                                                                                                    >  1:115174/1‑148 (MQ=255)
                                             aGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCa                                                                                                  >  2:1550966/1‑148 (MQ=255)
                                              gCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAg                                                                                                 >  1:1456610/1‑148 (MQ=255)
                                                    cATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGc                                                                                           >  1:2180328/1‑148 (MQ=255)
                                                      ttACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCAt                                                                                         >  2:1587889/1‑148 (MQ=255)
                                                       tACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATc                                                                                        >  2:2659079/1‑148 (MQ=255)
                                                         ccACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCaa                                                                                      <  1:2174828/148‑1 (MQ=255)
                                                           acaacaAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAAc                                                                                    <  2:2118807/148‑1 (MQ=255)
                                                           acaacaAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAAc                                                                                    <  2:1808447/148‑1 (MQ=255)
                                                             aacaaATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACAt                                                                                  >  2:1011081/1‑148 (MQ=255)
                                                             aacaaATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACAt                                                                                  <  1:265672/148‑1 (MQ=255)
                                                             aacaaATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACAt                                                                                  >  2:3147371/1‑148 (MQ=255)
                                                               caaATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCg                                                                                >  1:169424/1‑148 (MQ=255)
                                                                 aaTCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCAATGTAGGGATGGCGATTTCCCATACTTTGTGGTTACTTCACAGTTGCGATATATGCCATCAGACAATGCTCCAGAAAACTCAGCATGGCATCAAACATCGCt                                                                              >  2:1198201/1‑148 (MQ=255)
                                                                  aTCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCta                                                                             >  1:1813924/1‑148 (MQ=255)
                                                                   tCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCtat                                                                            <  1:549232/148‑1 (MQ=255)
                                                                     gTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTatat                                                                          <  1:3032964/148‑1 (MQ=255)
                                                                       aaTCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCg                                                                        >  2:2687615/1‑148 (MQ=255)
                                                                         tCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGAc                                                                      >  1:974827/1‑148 (MQ=255)
                                                                         tCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGAc                                                                      >  2:2612769/1‑148 (MQ=255)
                                                                         tCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGAc                                                                      >  2:1774535/1‑148 (MQ=255)
                                                                          cGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACa                                                                     >  2:347212/1‑148 (MQ=255)
                                                                             cTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATgc                                                                  >  1:1926719/1‑148 (MQ=255)
                                                                             cTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATgc                                                                  >  1:220939/1‑148 (MQ=255)
                                                                               tATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATgcgc                                                                <  1:1841924/148‑1 (MQ=255)
                                                                                     gAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCctct                                                          >  1:3138524/1‑148 (MQ=255)
                                                                                      aGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCctctc                                                         <  1:1031130/148‑1 (MQ=255)
                                                                                      aGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCctctc                                                         <  1:2810756/148‑1 (MQ=255)
                                                                                        tATTGTAAAAATCAAAAGGTTCCATGTAGGGATGTCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATTCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTg                                                       <  2:450591/148‑1 (MQ=255)
                                                                                        tATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTg                                                       >  1:1932492/1‑148 (MQ=255)
                                                                                         aTTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAATCATCGCTATAGCGACATGCGCCCCTCTCTgg                                                      >  2:3330778/1‑148 (MQ=255)
                                                                                         aTTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTgg                                                      >  1:2765429/1‑148 (MQ=255)
                                                                                           tGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGct                                                    <  1:716263/148‑1 (MQ=255)
                                                                                             aaaaCATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGctct                                                  <  1:9442/148‑1 (MQ=255)
                                                                                               aaCATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCttt                                                >  1:1772616/1‑148 (MQ=255)
                                                                                                aCATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCtttt                                               >  2:3219013/1‑148 (MQ=255)
                                                                                                   tCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTcgc                                            >  2:328232/1‑148 (MQ=255)
                                                                                                    cAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTcgcg                                           >  1:3113834/1‑148 (MQ=255)
                                                                                                    cAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTcgcg                                           <  2:2749549/148‑1 (MQ=255)
                                                                                                      aaaGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGc                                         <  2:2533157/148‑1 (MQ=255)
                                                                                                      aaaGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGc                                         <  1:1431307/148‑1 (MQ=255)
                                                                                                            gCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATaaa                                   >  2:1479929/1‑148 (MQ=255)
                                                                                                             ccATGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAg                                  <  1:2133295/148‑1 (MQ=255)
                                                                                                               aTGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTg                                >  2:1678965/1‑148 (MQ=255)
                                                                                                               aTGTAGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTg                                <  2:2242244/148‑1 (MQ=255)
                                                                                                                   aGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGcact                            >  2:234854/1‑148 (MQ=255)
                                                                                                                   aGGGATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGcact                            >  2:234754/1‑148 (MQ=255)
                                                                                                                     ggATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGcactca                          >  1:2372386/1‑148 (MQ=255)
                                                                                                                     ggATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGcactca                          >  1:425401/1‑148 (MQ=255)
                                                                                                                     ggATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGcactca                          >  1:118643/1‑148 (MQ=255)
                                                                                                                      gATGGCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGcactcac                         >  2:471012/1‑148 (MQ=255)
                                                                                                                          gCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTAtt                     <  2:582909/148‑1 (MQ=255)
                                                                                                                          gCGATTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCAGCAGACCATGATCAATACCACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTAtt                     >  1:3497441/1‑148 (MQ=255)
                                                                                                                             aTTTCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCg                  >  1:345698/1‑148 (MQ=255)
                                                                                                                              tttCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGc                 >  1:2105889/1‑148 (MQ=255)
                                                                                                                               ttCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCt                >  2:1659162/1‑148 (MQ=255)
                                                                                                                               ttCCCAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCt                <  2:2571522/148‑1 (MQ=255)
                                                                                                                                 cccAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGa              >  1:609743/1‑148 (MQ=255)
                                                                                                                                  ccAGACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGaa             >  2:196877/1‑148 (MQ=255)
                                                                                                                                     gACGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGaataa          <  2:334980/148‑1 (MQ=255)
                                                                                                                                      aCGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGaataaa         >  2:458168/1‑148 (MQ=255)
                                                                                                                                       cGTTGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGaataaat        <  1:2594539/148‑1 (MQ=255)
                                                                                                                                         ttGTGGTTACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAg      >  1:3527146/1‑148 (MQ=255)
                                                                                                                                             ggttACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGacac  >  1:1284952/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TTAACCAGTTCCGTAAGCTCCAGTGCCTGGCATAACGCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACAC  >  CP009273/2481830‑2482118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑