Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,499,568 C→A 100% D182Y (GAC→TAC)  ypdF ← Xaa‑Pro aminopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,499,5680CA99.4% 518.3 / ‑4.2 161D182Y (GAC→TAC) ypdFXaa‑Pro aminopeptidase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (67/93);  total (67/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 164 total aligned reads displayed.

ACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTTCTC  >  CP009273/2499423‑2499712
                                                                                                                                                 |                                                                                                                                                
aCCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTaaa                                                                                                                                                >  2:3210954/1‑148 (MQ=255)
    gCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAaga                                                                                                                                            <  1:3210920/148‑1 (MQ=255)
    gCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAaga                                                                                                                                            <  2:684602/148‑1 (MQ=255)
       aGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGc                                                                                                                                         <  2:1713878/148‑1 (MQ=255)
         gTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACACTGGTGTAAAAAGAGGCtt                                                                                                                                       >  1:2398837/1‑148 (MQ=255)
         gTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCtt                                                                                                                                       >  1:2398894/1‑148 (MQ=255)
           gcgcGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCtttt                                                                                                                                     <  1:273966/148‑1 (MQ=255)
           gcgcGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCtttt                                                                                                                                     <  1:2631464/148‑1 (MQ=255)
           gcgcGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCtttt                                                                                                                                     <  1:576122/148‑1 (MQ=255)
            cgcgTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCttttt                                                                                                                                    >  2:743152/1‑148 (MQ=255)
            cgcgTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCttttt                                                                                                                                    >  1:3348555/1‑148 (MQ=255)
            cgcgTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCttttt                                                                                                                                    >  1:1594437/1‑148 (MQ=255)
                    atCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGccc                                                                                                                            >  2:1878838/1‑148 (MQ=255)
                    atCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGccc                                                                                                                            <  2:204517/148‑1 (MQ=255)
                      cAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCctg                                                                                                                          <  1:793742/148‑1 (MQ=255)
                        gagCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCctgct                                                                                                                        <  1:909854/148‑1 (MQ=255)
                           cAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGgc                                                                                                                     >  1:2497353/1‑148 (MQ=255)
                           cAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGgc                                                                                                                     >  1:2497599/1‑148 (MQ=255)
                             gTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGgcgc                                                                                                                   <  1:3171091/148‑1 (MQ=255)
                                 cccTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATaa                                                                                                               >  2:1463148/1‑148 (MQ=255)
                                  ccTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATaaa                                                                                                              <  2:580538/148‑1 (MQ=255)
                                  ccTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATaaa                                                                                                              <  2:1502405/148‑1 (MQ=255)
                                   cTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAAc                                                                                                             <  2:3094310/148‑1 (MQ=255)
                                   cTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAAc                                                                                                             >  1:3258504/1‑148 (MQ=255)
                                        aCAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTc                                                                                                        <  2:1551956/148‑1 (MQ=255)
                                          aGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCa                                                                                                      >  1:2878965/1‑148 (MQ=255)
                                            cgcACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGt                                                                                                    >  2:134072/1‑148 (MQ=255)
                                                 cGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGc                                                                                               >  2:746569/1‑148 (MQ=255)
                                                   aaaTCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCgg                                                                                             >  2:2068973/1‑148 (MQ=255)
                                                   aaaTCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCgg                                                                                             >  2:1553737/1‑148 (MQ=255)
                                                    aaTCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGc                                                                                            <  2:2594000/148‑1 (MQ=255)
                                                     aTCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCt                                                                                           >  2:2731468/1‑148 (MQ=255)
                                                     aTCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCt                                                                                           >  1:1097322/1‑148 (MQ=255)
                                                      tCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTa                                                                                          >  1:3395774/1‑148 (MQ=255)
                                                      tCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTa                                                                                          >  1:2817054/1‑148 (MQ=255)
                                                       cGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTAt                                                                                         <  2:95635/148‑1 (MQ=255)
                                                       cGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTAt                                                                                         <  2:1631238/148‑1 (MQ=255)
                                                       cGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGCCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTAt                                                                                         <  2:1630825/148‑1 (MQ=255)
                                                       cGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGACGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTAt                                                                                         <  2:606000/148‑1 (MQ=255)
                                                             ggACAAACTCGCCCGCTGCACCACTCGTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTcgcg                                                                                   <  1:985357/147‑1 (MQ=255)
                                                               aCAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgct                                                                                 <  1:1953290/148‑1 (MQ=255)
                                                                cAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgctc                                                                                >  1:847041/1‑148 (MQ=255)
                                                                  aaCTCGCCCGCTGCAACAATCGTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgctcgc                                                                              <  2:1753926/148‑1 (MQ=255)
                                                                   aCTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgctcgct                                                                             >  2:3418063/1‑148 (MQ=255)
                                                                   aCTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgctcgct                                                                             >  2:1164473/1‑148 (MQ=255)
                                                                   aCTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgctcgct                                                                             >  2:3418099/1‑148 (MQ=255)
                                                                       gCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATc                                                                         >  2:1865002/1‑148 (MQ=255)
                                                                       gCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAAGGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATc                                                                         >  2:2597798/1‑148 (MQ=255)
                                                                       gCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGGGCGGCCGCGCCCCACGCCAGCCACTGGCGACAATGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATc                                                                         <  1:2394310/148‑1 (MQ=255)
                                                                         ccGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATccc                                                                       <  1:2172224/148‑1 (MQ=255)
                                                                            cTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGc                                                                    <  1:1137213/148‑1 (MQ=255)
                                                                            cTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGc                                                                    <  1:240447/148‑1 (MQ=255)
                                                                               caacaaTCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTg                                                                 >  2:3229879/1‑148 (MQ=255)
                                                                                aacaaTCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGa                                                                >  2:1806368/1‑148 (MQ=255)
                                                                                  caaTCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAAt                                                              >  2:3187686/1‑148 (MQ=255)
                                                                                  caaTCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAAt                                                              >  1:1396037/1‑148 (MQ=255)
                                                                                    aTCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCCCGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATaa                                                            <  1:84275/148‑1 (MQ=255)
                                                                                    aTCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACATTGGTTTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATaa                                                            <  1:86854/148‑1 (MQ=255)
                                                                                    aTCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATaa                                                            >  2:1488364/1‑148 (MQ=255)
                                                                                    aTCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATaa                                                            <  1:84164/148‑1 (MQ=255)
                                                                                     tCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATaaa                                                           >  2:145641/1‑148 (MQ=255)
                                                                                     tCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATaaa                                                           >  2:107267/1‑148 (MQ=255)
                                                                                      cTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAg                                                          <  1:743152/148‑1 (MQ=255)
                                                                                       ttGTCGCTGTCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGAATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAgc                                                         <  1:1937732/148‑1 (MQ=255)
                                                                                            gCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGa                                                    <  1:2618298/148‑1 (MQ=255)
                                                                                             cTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGTGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGaa                                                   <  2:909318/148‑1 (MQ=255)
                                                                                             cTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAAGGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGaa                                                   <  2:909090/148‑1 (MQ=255)
                                                                                              tGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCGGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAat                                                  <  1:1318010/148‑1 (MQ=255)
                                                                                               ggCTTTGCGGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAAGGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAata                                                 <  1:457284/148‑1 (MQ=255)
                                                                                               ggCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAata                                                 <  2:190485/148‑1 (MQ=255)
                                                                                                  tttGCCGTGCGGCAGCGCCCCACGCCAGCCACGGGCGAAAATGGTTTAAAAAGTGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGc                                              <  1:2118594/148‑1 (MQ=255)
                                                                                                  tttGCCGTGCGGCAGCCCCCCCCGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGATTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGc                                              <  1:774647/148‑1 (MQ=255)
                                                                                                   ttGCCGTGCGGCCGCCCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCGGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGct                                             <  1:1286358/148‑1 (MQ=255)
                                                                                                   ttGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGct                                             >  2:2966978/1‑148 (MQ=255)
                                                                                                    tGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACATTGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGctc                                            <  1:3176397/148‑1 (MQ=255)
                                                                                                        gTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGctc                                            <  1:3526009/144‑1 (MQ=255)
                                                                                                         tGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCAc                                       >  1:2115101/1‑148 (MQ=255)
                                                                                                           cGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACcg                                     <  2:2510179/148‑1 (MQ=255)
                                                                                                            ggCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACcgc                                    <  2:860701/148‑1 (MQ=255)
                                                                                                              cAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGa                                  <  2:1431167/148‑1 (MQ=255)
                                                                                                              cAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGa                                  <  1:2223532/148‑1 (MQ=255)
                                                                                                              cAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGa                                  >  2:1002721/1‑148 (MQ=255)
                                                                                                                      cACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAAt                          <  1:2678988/148‑1 (MQ=255)
                                                                                                                      cACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAAt                          <  1:134072/148‑1 (MQ=255)
                                                                                                                       aCGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATc                         <  2:1559749/148‑1 (MQ=255)
                                                                                                                        cGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATcc                        <  2:2497599/148‑1 (MQ=255)
                                                                                                                        cGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATcc                        <  2:2497353/148‑1 (MQ=255)
                                                                                                                        cGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATcc                        <  1:2351109/148‑1 (MQ=255)
                                                                                                                         gcccgccaCTGGCGACACTGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATccc                       <  1:2535584/148‑1 (MQ=255)
                                                                                                                          ccagccaCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCca                      <  1:2946074/148‑1 (MQ=255)
                                                                                                                               caCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACaggc                 >  2:2278744/1‑148 (MQ=255)
                                                                                                                                 cTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACaggcga               >  1:1833819/1‑148 (MQ=255)
                                                                                                                                    gCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACaggcgaggc            >  1:1034228/1‑148 (MQ=255)
                                                                                                                                    gCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGATCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCACACaggcgaggc            >  1:1034229/1‑148 (MQ=255)
                                                                                                                                     cGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACaggcgaggcg           >  1:1694077/1‑148 (MQ=255)
                                                                                                                                      gACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCgg          <  1:447831/148‑1 (MQ=255)
                                                                                                                                       aCAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTAGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGa         >  2:1026937/1‑148 (MQ=255)
                                                                                                                                              tgtAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTtctc  >  2:3510380/1‑148 (MQ=255)
                                                                                                                                              tgtAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTtctc  >  1:409953/1‑148 (MQ=255)
                                                                                                                                              tgtAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTtctc  <  1:2922862/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTTCTC  >  CP009273/2499423‑2499712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑