Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,512,279 G→T 51.4% D112Y (GAT→TAT)  yfeC → DUF1323 family putative DNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,512,2790GT51.4% 43.6 / inf 197D112Y (GAT→TAT) yfeCDUF1323 family putative DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (54/42);  new base T (57/44);  total (111/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 199 total aligned reads displayed.

GAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAATTTCCCGGCGTGAAAGGCGGTCG  >  CP009273/2512132‑2512425
                                                                                                                                                   |                                                                                                                                                  
gaAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTcgcg                                                                                                                                                    <  1:1557384/148‑1 (MQ=255)
       cTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCaa                                                                                                                                             <  1:3186742/148‑1 (MQ=255)
       cTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCaa                                                                                                                                             >  1:379882/1‑148 (MQ=255)
       cTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCaa                                                                                                                                             <  2:2285155/148‑1 (MQ=255)
       cTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCaa                                                                                                                                             <  1:2301322/148‑1 (MQ=255)
       cTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCaa                                                                                                                                             <  1:2301096/148‑1 (MQ=255)
        tGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCaaa                                                                                                                                            >  1:2340814/1‑148 (MQ=255)
         gCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAaat                                                                                                                                           <  2:1180282/148‑1 (MQ=255)
          cccTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAaata                                                                                                                                          <  1:3372487/148‑1 (MQ=255)
           ccTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAaataa                                                                                                                                         <  1:1199089/148‑1 (MQ=255)
            cTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAaataat                                                                                                                                        <  2:1919136/148‑1 (MQ=255)
            cTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAaataat                                                                                                                                        <  2:1092444/148‑1 (MQ=255)
                cTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGa                                                                                                                                    <  1:2443556/148‑1 (MQ=255)
                     gATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGa                                                                                                                               <  1:592887/148‑1 (MQ=255)
                     gATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGa                                                                                                                               <  2:185083/148‑1 (MQ=255)
                     gATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGa                                                                                                                               <  2:1857590/148‑1 (MQ=255)
                       tGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGAtt                                                                                                                             <  1:3037805/148‑1 (MQ=255)
                           ccgcTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGc                                                                                                                         <  1:3462832/148‑1 (MQ=255)
                            cgcTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCa                                                                                                                        <  1:240393/148‑1 (MQ=255)
                                 gAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATaaa                                                                                                                   <  1:2045860/148‑1 (MQ=255)
                                  aaGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATaaaa                                                                                                                  <  1:3558755/148‑1 (MQ=255)
                                  aaGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATaaaa                                                                                                                  >  1:3180064/1‑148 (MQ=255)
                                  aaGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATaaaa                                                                                                                  >  1:3180020/1‑148 (MQ=255)
                                  aaGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATaaaa                                                                                                                  >  1:217935/1‑148 (MQ=255)
                                      aTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGac                                                                                                              >  2:564717/1‑148 (MQ=255)
                                      aTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGac                                                                                                              <  1:2814236/148‑1 (MQ=255)
                                      aTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGac                                                                                                              >  2:1256277/1‑148 (MQ=255)
                                      aTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGac                                                                                                              >  2:1396926/1‑148 (MQ=255)
                                      aTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGac                                                                                                              >  2:2229543/1‑148 (MQ=255)
                                        tGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGacca                                                                                                            >  1:1275818/1‑148 (MQ=255)
                                        tGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGacca                                                                                                            >  1:3228854/1‑148 (MQ=255)
                                         gCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGaccac                                                                                                           <  2:1272579/148‑1 (MQ=255)
                                            ggTGACACTTGCGAAAGAGATGACTCCAGTTTAACAAATAAAGTTTACATCCTTGCTTCTGCGGGAAGTGATTATGTGATTGTTTCAACGCTTAGGGATTCGCTATATCAATTAATATTAAAAGATTACGCAATAAAATGACATCCga                                                                                                        <  2:1282962/148‑1 (MQ=255)
                                              tGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCgaag                                                                                                      <  2:1103736/148‑1 (MQ=255)
                                               gACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCgaaga                                                                                                     <  2:647480/148‑1 (MQ=255)
                                                      gCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCt                                                                                              >  2:1878415/1‑148 (MQ=255)
                                                        gAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGa                                                                                            <  2:2653488/148‑1 (MQ=255)
                                                        gAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGa                                                                                            >  2:731054/1‑148 (MQ=255)
                                                                 gACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCgg                                                                                   <  2:968291/148‑1 (MQ=255)
                                                                   cGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGgtg                                                                                 <  1:2598827/148‑1 (MQ=255)
                                                                   cGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGgtg                                                                                 >  1:1784938/1‑148 (MQ=255)
                                                                   cGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGgtg                                                                                 >  1:649027/1‑148 (MQ=255)
                                                                    gCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGgtgt                                                                                <  1:1992032/148‑1 (MQ=255)
                                                                       aGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGATGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGc                                                                             <  1:1615900/148‑1 (MQ=255)
                                                                           gAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTaaa                                                                         >  1:859805/1‑148 (MQ=255)
                                                                           gAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTaaa                                                                         >  1:809071/1‑148 (MQ=255)
                                                                             aCAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTaaaca                                                                       <  2:3393547/148‑1 (MQ=255)
                                                                              cAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTaaacaa                                                                      >  1:2882439/1‑148 (MQ=255)
                                                                                aaaaCAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTaaacaaac                                                                    >  1:1579050/1‑148 (MQ=255)
                                                                                aaaaCAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTaaacaaac                                                                    >  1:2651647/1‑148 (MQ=255)
                                                                                aaaaCAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTaaacaaac                                                                    >  2:2939569/1‑148 (MQ=255)
                                                                                aaaaCAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTaaacaaac                                                                    >  2:2716922/1‑148 (MQ=255)
                                                                                 aaaCAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACt                                                                   >  2:2344995/1‑148 (MQ=255)
                                                                                 aaaCAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACt                                                                   <  1:1305489/148‑1 (MQ=255)
                                                                                    cAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGtt                                                                >  1:1035218/1‑148 (MQ=255)
                                                                                     aGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTa                                                               <  1:2540970/148‑1 (MQ=255)
                                                                                        ttACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAAcc                                                            >  2:3104524/1‑148 (MQ=255)
                                                                                        ttACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAAcc                                                            >  1:1710315/1‑148 (MQ=255)
                                                                                        ttACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAAcc                                                            >  2:2455530/1‑148 (MQ=255)
                                                                                        ttACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAAcc                                                            >  2:770369/1‑148 (MQ=255)
                                                                                         tACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCg                                                           <  2:379882/148‑1 (MQ=255)
                                                                                          aCATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGt                                                          <  2:1997056/148‑1 (MQ=255)
                                                                                          aCATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGt                                                          >  2:3046990/1‑148 (MQ=255)
                                                                                           cATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGtt                                                         >  2:1653103/1‑148 (MQ=255)
                                                                                           cATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGtt                                                         >  2:1653420/1‑148 (MQ=255)
                                                                                              ccTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGa                                                      <  1:1101561/148‑1 (MQ=255)
                                                                                                 tGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCa                                                   >  1:2994839/1‑148 (MQ=255)
                                                                                                 tGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCa                                                   <  2:2897585/148‑1 (MQ=255)
                                                                                                     tCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAgaga                                               <  2:2041603/148‑1 (MQ=255)
                                                                                                       tGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGaaa                                             >  2:1543906/1‑148 (MQ=255)
                                                                                                        gCGGGAAGGGATTATCGTATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAATAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGaaaa                                            >  1:541676/1‑148 (MQ=255)
                                                                                                           ggAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAgg                                         <  1:1352023/148‑1 (MQ=255)
                                                                                                                ggATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGa                                    >  1:3128075/1‑148 (MQ=255)
                                                                                                                 gATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGaa                                   >  2:1900167/1‑148 (MQ=255)
                                                                                                                 gATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGaa                                   >  1:181355/1‑148 (MQ=255)
                                                                                                                  aTTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGATGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGATGGaaa                                  >  2:432239/1‑148 (MQ=255)
                                                                                                                  aTTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGaaa                                  >  2:1963818/1‑148 (MQ=255)
                                                                                                                   ttATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGaaaa                                 >  2:1603270/1‑148 (MQ=255)
                                                                                                                   ttATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGaaaa                                 >  1:3306826/1‑148 (MQ=255)
                                                                                                                       cGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGa                             >  1:3166602/1‑148 (MQ=255)
                                                                                                                       cGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGa                             >  1:3167610/1‑148 (MQ=255)
                                                                                                                          aTTGTTACCACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGaaaa                          <  2:477815/148‑1 (MQ=255)
                                                                                                                          aTTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGaaaa                          >  2:1779438/1‑148 (MQ=255)
                                                                                                                           ttGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAATATTACGCAATAAAATGACCACCTAAGAACTGGCTGAATGCCTCGGTGTGGGTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGaaaaa                         <  2:363117/148‑1 (MQ=255)
                                                                                                                                 cAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAATTTccc                   <  2:137500/148‑1 (MQ=255)
                                                                                                                                   aCGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAAAAAAATGACCACCCAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAATTTCCCgg                 >  1:3498386/1‑148 (MQ=255)
                                                                                                                                    cGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAATTTCCCGGc                >  2:1637294/1‑148 (MQ=255)
                                                                                                                                     gCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAATTTCCCGGCg               <  1:800692/148‑1 (MQ=255)
                                                                                                                                      cTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAATTTCCCGGCGt              >  2:2696811/1‑148 (MQ=255)
                                                                                                                                      cTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAATTTCCCGGCGt              >  1:2627286/1‑148 (MQ=255)
                                                                                                                                      cTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAATTTCCCGGCGt              >  1:3156648/1‑148 (MQ=255)
                                                                                                                                        tAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAATTTCCCGGCGTGa            >  1:2531755/1‑148 (MQ=255)
                                                                                                                                        tAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAATTTCCCGGCGTGa            >  2:1000425/1‑148 (MQ=255)
                                                                                                                                          gggATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAATTTCCCGGCGTGaaa          >  1:850486/1‑148 (MQ=255)
                                                                                                                                          gggATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAATTTCCCGGCGTGaaa          <  2:181355/148‑1 (MQ=255)
                                                                                                                                           ggATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAATTTCCCGGCGTGAAAg         <  2:810945/148‑1 (MQ=255)
                                                                                                                                               tCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAGCGGAAAAATTTCCCGGCGTGAAAGGCgg     >  2:1788463/1‑148 (MQ=255)
                                                                                                                                               tCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAATTTCCCGGCGTGAAAGGCgg     >  2:2986617/1‑148 (MQ=255)
                                                                                                                                               tCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAATTTCCCGGCGTGAAAGGCgg     <  1:468206/148‑1 (MQ=255)
                                                                                                                                                  cgATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAACAATTTCCCGGCGTGAAAGGCGGTCg  >  2:1227454/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAATTTCCCGGCGTGAAAGGCGGTCG  >  CP009273/2512132‑2512425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑