Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,524,056 C→A 93.8% A179A (GCG→GCT)  zipA ← FtsZ stabilizer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,524,0560CA93.8% 596.8 / 28.6 209A179A (GCG→GCT) zipAFtsZ stabilizer
Reads supporting (aligned to +/- strand):  ref base C (7/6);  new base A (107/89);  total (114/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

TATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGC  >  CP009273/2523911‑2524200
                                                                                                                                                 |                                                                                                                                                
   tCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAc                                                                                                                                             <  2:3362734/148‑1 (MQ=255)
   tCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAc                                                                                                                                             >  2:2946860/1‑148 (MQ=255)
            cAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGTGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAcaggctcagg                                                                                                                                    <  2:689900/148‑1 (MQ=255)
            cAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTAcaggctcagg                                                                                                                                    <  1:2900369/148‑1 (MQ=255)
            cAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAcaggctcagg                                                                                                                                    >  2:2396090/1‑148 (MQ=255)
             aaaaaTGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAcaggctcaggc                                                                                                                                   <  2:1453155/148‑1 (MQ=255)
              aaaaTGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAcaggctcaggct                                                                                                                                  >  1:3435968/1‑148 (MQ=255)
               aaaTGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCtg                                                                                                                                 >  1:688742/1‑148 (MQ=255)
                aaTGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCtgt                                                                                                                                <  2:3298576/148‑1 (MQ=255)
                 aTGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCtgtg                                                                                                                               >  1:404573/1‑148 (MQ=255)
                  tGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTgtgg                                                                                                                              <  1:3496635/148‑1 (MQ=255)
                  tGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTgtgg                                                                                                                              >  2:117661/1‑148 (MQ=255)
                  tGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTgtgg                                                                                                                              >  2:3560044/1‑148 (MQ=255)
                   gAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTgtggt                                                                                                                             >  1:3103293/1‑148 (MQ=255)
                           cTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTAc                                                                                                                     >  2:1861529/1‑148 (MQ=255)
                            ttgttgAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACg                                                                                                                    >  1:938444/1‑148 (MQ=255)
                                tgAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGTTGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGtt                                                                                                                <  1:608797/148‑1 (MQ=255)
                                tgAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGtt                                                                                                                >  2:2615202/1‑148 (MQ=255)
                                 gAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTc                                                                                                               <  1:1866067/148‑1 (MQ=255)
                                    tGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGc                                                                                                            <  1:1922877/148‑1 (MQ=255)
                                    tGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGc                                                                                                            <  1:1666727/148‑1 (MQ=255)
                                       tGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAgg                                                                                                         >  2:779869/1‑148 (MQ=255)
                                        gTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGc                                                                                                        <  1:3057642/148‑1 (MQ=255)
                                             gaagCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGaa                                                                                                   <  1:2290338/148‑1 (MQ=255)
                                             gaagCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGaa                                                                                                   >  1:2563055/1‑148 (MQ=255)
                                              aagCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGaaa                                                                                                  >  1:686370/1‑148 (MQ=255)
                                               agCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAg                                                                                                 <  1:765845/148‑1 (MQ=255)
                                                gCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGc                                                                                                <  1:3026871/148‑1 (MQ=255)
                                                gCAGTTCACCGTTTAGCTCGCGACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGc                                                                                                <  2:461185/148‑1 (MQ=255)
                                                 cAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTc                                                                                                               >  1:2858338/1‑132 (MQ=255)
                                                   gTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCtg                                                                                             <  1:3152607/148‑1 (MQ=255)
                                                    ttCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCtgt                                                                                            >  1:3422058/1‑148 (MQ=255)
                                                     tCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCtgtt                                                                                           >  2:2341074/1‑148 (MQ=255)
                                                     tCACCGAATAGCTCGCTACCGAGAAGCGCCGCGACGTTCAAGATAATCACCGCATCTATGCGCTTCGGATAATCCATAACTGGAGCACGTACAGCTACAGGCTCAGGCTCTGTTGCAGCTACGGGTTCTGCAGGCTGGAAAGCCtctt                                                                                           >  2:1639082/1‑148 (MQ=255)
                                                        ccGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTtgtg                                                                                        >  2:1301913/1‑148 (MQ=255)
                                                            ttAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCgg                                                                                    <  2:1265538/148‑1 (MQ=255)
                                                               gCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTg                                                                                 >  2:15699/1‑148 (MQ=255)
                                                               gCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTg                                                                                 >  2:564011/1‑148 (MQ=255)
                                                               gCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTg                                                                                 <  1:158181/148‑1 (MQ=255)
                                                                cTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGc                                                                                >  1:1438683/1‑148 (MQ=255)
                                                                cTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGc                                                                                >  1:3211367/1‑148 (MQ=255)
                                                                 tCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCg                                                                               <  1:2523273/148‑1 (MQ=255)
                                                                   gCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGt                                                                             >  2:250531/1‑148 (MQ=255)
                                                                      aCCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCt                                                                          <  1:2491187/148‑1 (MQ=255)
                                                                       ccGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTg                                                                         >  2:230278/1‑148 (MQ=255)
                                                                         gTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGaa                                                                       <  1:1645593/148‑1 (MQ=255)
                                                                           gATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATg                                                                     <  2:3178082/148‑1 (MQ=255)
                                                                               cgccgcGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGcaca                                                                 <  1:2946860/148‑1 (MQ=255)
                                                                                 ccgcGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAgg                                                               >  2:2879611/1‑148 (MQ=255)
                                                                                  cgcgACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGc                                                              <  2:3506071/148‑1 (MQ=255)
                                                                                   gcgACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCt                                                             >  1:2845562/1‑148 (MQ=255)
                                                                                    cgACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTg                                                            >  2:1386717/1‑148 (MQ=255)
                                                                                       cGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTgcgg                                                         <  2:1089869/148‑1 (MQ=255)
                                                                                          tCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGgcgc                                                      <  2:806639/148‑1 (MQ=255)
                                                                                          tCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGgcgc                                                      >  1:2655439/1‑148 (MQ=255)
                                                                                          tCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGgcgc                                                      <  1:2408473/148‑1 (MQ=255)
                                                                                           cATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGACTGCACAGGCTGCGGCGCt                                                     >  2:2461758/1‑148 (MQ=255)
                                                                                            aTGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTg                                                    <  2:32052/148‑1 (MQ=255)
                                                                                            aTGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTg                                                    <  1:2315702/148‑1 (MQ=255)
                                                                                            aTGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTg                                                    <  2:2370604/148‑1 (MQ=255)
                                                                                            aTGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTg                                                    <  2:2370892/148‑1 (MQ=255)
                                                                                               aTAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGgcg                                                 >  2:2604470/1‑148 (MQ=255)
                                                                                                 aaTCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGcgcg                                               >  2:2858855/1‑148 (MQ=255)
                                                                                                 aaTCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGcgcg                                               >  2:2856471/1‑148 (MQ=255)
                                                                                                  aTCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGa                                              >  2:638663/1‑148 (MQ=255)
                                                                                                   tCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGAc                                             >  2:161137/1‑148 (MQ=255)
                                                                                                   tCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGAc                                             >  2:2717139/1‑148 (MQ=255)
                                                                                                      ccGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCTGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCtg                                          >  1:2093286/1‑148 (MQ=255)
                                                                                                      ccGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCtg                                          >  2:866942/1‑148 (MQ=255)
                                                                                                       cGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCtgt                                         >  1:2978298/1‑148 (MQ=255)
                                                                                                           tcttTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCg                                     >  1:127618/1‑148 (MQ=255)
                                                                                                             tttGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGaa                                   >  1:2549539/1‑148 (MQ=255)
                                                                                                             tttGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGaa                                   >  1:761405/1‑148 (MQ=255)
                                                                                                                   cTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGgc                             >  1:114663/1‑148 (MQ=255)
                                                                                                                    ttCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGgct                            >  2:3232299/1‑148 (MQ=255)
                                                                                                                     tCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGgctg                           >  1:2165508/1‑148 (MQ=255)
                                                                                                                     tCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGgctg                           >  2:599044/1‑148 (MQ=255)
                                                                                                                     tCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGgctg                           >  2:732885/1‑148 (MQ=255)
                                                                                                                     tCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGgctg                           >  1:89332/1‑148 (MQ=255)
                                                                                                                     tCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGgctg                           >  1:662591/1‑148 (MQ=255)
                                                                                                                        gTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGctgctg                        >  2:2445287/1‑148 (MQ=255)
                                                                                                                         tttATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCtgctgc                       >  1:725746/1‑148 (MQ=255)
                                                                                                                         tttATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCtgctgc                       >  2:66158/1‑148 (MQ=255)
                                                                                                                          ttATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCa                      <  2:2186104/148‑1 (MQ=255)
                                                                                                                            aTCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAAc                    >  2:36149/1‑148 (MQ=255)
                                                                                                                             tCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACg                   >  2:2540653/1‑148 (MQ=255)
                                                                                                                              ccATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACgg                  >  1:2979998/1‑148 (MQ=255)
                                                                                                                              ccATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACgg                  <  2:42290/148‑1 (MQ=255)
                                                                                                                               cATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGc                 <  2:1908080/148‑1 (MQ=255)
                                                                                                                               cATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGc                 <  1:2444559/148‑1 (MQ=255)
                                                                                                                                aTAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTAGGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCt                >  1:1057958/1‑148 (MQ=255)
                                                                                                                                 tAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTg               <  1:2341074/148‑1 (MQ=255)
                                                                                                                                 tAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTg               <  2:2979998/148‑1 (MQ=255)
                                                                                                                                  aaCTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGt              <  2:515482/148‑1 (MQ=255)
                                                                                                                                    cTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCACCGGCTGTTc            >  1:1015812/1‑148 (MQ=255)
                                                                                                                                     tGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCa           >  2:2803227/1‑148 (MQ=255)
                                                                                                                                        aGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGc        <  2:404573/148‑1 (MQ=255)
                                                                                                                                           aGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGc     >  2:3525196/1‑148 (MQ=255)
                                                                                                                                             gTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCgg   >  1:2682005/1‑148 (MQ=255)
                                                                                                                                              ttCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGc  <  2:1916818/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGC  >  CP009273/2523911‑2524200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑