Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,552,461 A→G 52.4% N82S (AAT→AGT)  intZ → CPZ‑55 prophage; putative phage integrase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,552,4610AG52.4% 62.7 / 276.6 189N82S (AAT→AGT) intZCPZ‑55 prophage; putative phage integrase
Reads supporting (aligned to +/- strand):  ref base A (48/42);  new base G (55/44);  total (103/86)
Fisher's exact test for biased strand distribution p-value = 7.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 195 total aligned reads displayed.

TGCTGGTGCATCCCAACGGTTCGAAATACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGC  >  CP009273/2552314‑2552608
                                                                                                                                                   |                                                                                                                                                   
tGCTGGTGCATCCCAACGGTTCGAAATACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCaa                                                                                                                                                     <  1:2949411/148‑1 (MQ=255)
tGCTGGTGCATCCCAACGGTTCGAAATACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCaa                                                                                                                                                     <  2:359006/148‑1 (MQ=255)
       gCATCCCAACGGTTCGAAATACTGGCGATTCGGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAAtt                                                                                                                                              <  1:1035197/148‑1 (MQ=255)
        cATCCCAACGGTTCGAAATACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTa                                                                                                                                             >  2:1817908/1‑148 (MQ=255)
          tCCCAACGGTTCGAAATACTGGCGATTCAGGTATTGGCTGCATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAAc                                                                                                                                           <  1:1459334/148‑1 (MQ=255)
           cccAACGGTTCGAAATACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCTCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAAcc                                                                                                                                          >  1:1221135/1‑148 (MQ=255)
           cccAACGGTTCGAAATACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCAGTGGAGTTTACCCGCTAATCACACTCAAAGAAGAACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAAcc                                                                                                                                          >  2:2619974/1‑148 (MQ=255)
             cAACGGTTCGAAATACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCa                                                                                                                                        >  2:2447154/1‑148 (MQ=255)
              aaCGGTTCGAAATACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAg                                                                                                                                       >  2:2335399/1‑148 (MQ=255)
               aCGGTTCGAAATACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGt                                                                                                                                      >  1:2070195/1‑148 (MQ=255)
                   ttCGAAATACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGaa                                                                                                                                  >  1:1249665/1‑148 (MQ=255)
                   ttCGAAATACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGaa                                                                                                                                  >  2:3041396/1‑148 (MQ=255)
                       aaaTACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAg                                                                                                                              <  1:1604867/148‑1 (MQ=255)
                       aaaTACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAg                                                                                                                              <  1:3350468/148‑1 (MQ=255)
                       aaaTACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAg                                                                                                                              <  2:2993697/148‑1 (MQ=255)
                          tACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGccc                                                                                                                           >  2:732132/1‑148 (MQ=255)
                                  aTTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGaa                                                                                                                   >  1:1313174/1‑148 (MQ=255)
                                  aTTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGaa                                                                                                                   >  2:1947217/1‑148 (MQ=255)
                                         tATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCa                                                                                                            <  2:2786089/148‑1 (MQ=255)
                                           ttGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCAtt                                                                                                          <  2:975976/148‑1 (MQ=255)
                                            tGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCACGGAAAAAGCCATTg                                                                                                         >  2:2661951/1‑148 (MQ=255)
                                             ggCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGa                                                                                                        >  1:3235036/1‑148 (MQ=255)
                                             ggCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGa                                                                                                        >  1:3234795/1‑148 (MQ=255)
                                             ggCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGa                                                                                                        >  1:3117444/1‑148 (MQ=255)
                                               cTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATg                                                                                                      <  1:2199837/148‑1 (MQ=255)
                                                tGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATgc                                                                                                     >  2:318866/1‑148 (MQ=255)
                                                  aaTAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATgcgc                                                                                                   >  1:1677560/1‑148 (MQ=255)
                                                     aaaCGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCa                                                                                                >  2:1570395/1‑148 (MQ=255)
                                                     aaaCGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCa                                                                                                >  2:1572087/1‑148 (MQ=255)
                                                      aaCGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCaa                                                                                               >  2:1749424/1‑148 (MQ=255)
                                                       aCGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAAc                                                                                              >  1:1074677/1‑148 (MQ=255)
                                                       aCGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAAc                                                                                              >  2:3482887/1‑148 (MQ=255)
                                                         gagaGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACAt                                                                                            >  1:3353778/1‑148 (MQ=255)
                                                         gagaGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACAt                                                                                            <  1:2219288/148‑1 (MQ=255)
                                                         gagaGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACAt                                                                                            <  1:1113021/148‑1 (MQ=255)
                                                            agTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATggc                                                                                         >  1:442446/1‑148 (MQ=255)
                                                            agTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATggc                                                                                         <  1:2253908/148‑1 (MQ=255)
                                                            agTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATggc                                                                                         >  1:162988/1‑148 (MQ=255)
                                                            agTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATggc                                                                                         >  1:117236/1‑148 (MQ=255)
                                                              tttttGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATggcgg                                                                                       >  1:869968/1‑148 (MQ=255)
                                                              tttttGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATggcgg                                                                                       >  2:202388/1‑148 (MQ=255)
                                                                   gCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGa                                                                                  <  2:1168724/148‑1 (MQ=255)
                                                                      ggCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGAttt                                                                               >  1:155446/1‑148 (MQ=255)
                                                                       gCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTa                                                                              >  2:2407275/1‑148 (MQ=255)
                                                                       gCCATTGGAGTTTACCCGATAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTTTTGCAAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGATAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTa                                                                              <  1:246824/148‑1 (MQ=255)
                                                                        ccATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTaa                                                                             <  1:916408/148‑1 (MQ=255)
                                                                        ccATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTaa                                                                             <  2:1160741/148‑1 (MQ=255)
                                                                          aTTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTaaga                                                                           <  1:1852328/148‑1 (MQ=255)
                                                                           ttGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTaagaa                                                                          >  1:25760/1‑148 (MQ=255)
                                                                           ttGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTaagaa                                                                          >  1:1831403/1‑148 (MQ=255)
                                                                            tGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTaagaag                                                                         >  1:2520731/1‑148 (MQ=255)
                                                                             ggAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAgg                                                                        <  2:1656165/148‑1 (MQ=255)
                                                                              gAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGt                                                                       >  1:1028745/1‑148 (MQ=255)
                                                                                gTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTgg                                                                     <  1:3478575/148‑1 (MQ=255)
                                                                                 tttACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGc                                                                    >  1:2052528/1‑148 (MQ=255)
                                                                                    aCCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGa                                                                 <  2:1281470/148‑1 (MQ=255)
                                                                                      ccGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAg                                                               <  2:3284371/148‑1 (MQ=255)
                                                                                      ccGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAg                                                               >  1:1401848/1‑148 (MQ=255)
                                                                                        gCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGAc                                                             <  1:2027421/148‑1 (MQ=255)
                                                                                         cTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACt                                                            <  1:2903155/148‑1 (MQ=255)
                                                                                          tAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTg                                                           <  2:625670/148‑1 (MQ=255)
                                                                                           aaTCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTgg                                                          >  1:828256/1‑148 (MQ=255)
                                                                                              cacaCTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGttt                                                       >  1:3231487/1‑148 (MQ=255)
                                                                                              cacaCTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGttt                                                       >  1:3314841/1‑148 (MQ=255)
                                                                                               acacTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTg                                                      >  2:3538335/1‑148 (MQ=255)
                                                                                                 acTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCt                                                    >  2:1451980/1‑148 (MQ=255)
                                                                                                 acTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCt                                                    >  1:299805/1‑148 (MQ=255)
                                                                                                    cAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTAcc                                                 <  2:2052528/148‑1 (MQ=255)
                                                                                                     aaaGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATTGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCa                                                >  1:400356/1‑148 (MQ=255)
                                                                                                      aagaagCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAg                                               <  1:1451980/148‑1 (MQ=255)
                                                                                                        gaagCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAggg                                             >  2:1343918/1‑148 (MQ=255)
                                                                                                            cACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTgg                                         >  1:3255665/1‑148 (MQ=255)
                                                                                                             aCGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGa                                        <  1:1522106/148‑1 (MQ=255)
                                                                                                              cGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAg                                       <  2:1313174/148‑1 (MQ=255)
                                                                                                               gCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAgg                                      <  1:1750293/148‑1 (MQ=255)
                                                                                                               gCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAgg                                      <  1:732132/148‑1 (MQ=255)
                                                                                                                cAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGAAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAggg                                     <  1:208043/148‑1 (MQ=255)
                                                                                                                cAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAggg                                     <  2:3154688/148‑1 (MQ=255)
                                                                                                                cAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAggg                                     <  1:783299/148‑1 (MQ=255)
                                                                                                                   gcgcgcCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGggtgg                                  >  1:1797100/1‑148 (MQ=255)
                                                                                                                   gcgcgcCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGggtgg                                  >  1:1631659/1‑148 (MQ=255)
                                                                                                                   gcgcgcCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGggtgg                                  >  1:390568/1‑148 (MQ=255)
                                                                                                                    cgcgcCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGggtggt                                 <  2:2766930/148‑1 (MQ=255)
                                                                                                                     gcgcCACGGAGAGCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTc                                <  2:2705215/148‑1 (MQ=255)
                                                                                                                     gcgcCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTc                                <  2:1186063/148‑1 (MQ=255)
                                                                                                                        ccACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGa                             <  2:1009335/148‑1 (MQ=255)
                                                                                                                        ccACGGAGAGCAGATCCCTTATTGCCAAGGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGa                             <  2:1011923/148‑1 (MQ=255)
                                                                                                                           cGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATc                          <  1:2407275/148‑1 (MQ=255)
                                                                                                                            ggAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATcc                         <  2:1086986/148‑1 (MQ=255)
                                                                                                                            ggAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATcc                         <  1:2335399/148‑1 (MQ=255)
                                                                                                                              agagCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCCAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTa                       <  1:594650/148‑1 (MQ=255)
                                                                                                                              agagCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTa                       >  2:347740/1‑148 (MQ=255)
                                                                                                                                agCAGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATg                     >  1:364638/1‑148 (MQ=255)
                                                                                                                                   aGATCCCTTATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCaa                  >  2:1247940/1‑148 (MQ=255)
                                                                                                                                       cccTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACa              <  1:1947217/148‑1 (MQ=255)
                                                                                                                                         cTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAgg            <  1:2355847/148‑1 (MQ=255)
                                                                                                                                          ttATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGt           >  1:2174860/1‑148 (MQ=255)
                                                                                                                                          ttATTGCCAGTGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGt           >  1:1930162/1‑148 (MQ=255)
                                                                                                                                            aTTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCa         >  1:2764755/1‑148 (MQ=255)
                                                                                                                                                   aTGGAAGTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGc  <  2:2191729/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGCTGGTGCATCCCAACGGTTCGAAATACTGGCGATTCAGGTATTGGCTGAATAAACGAGAGTTTTTGCAGGCCATTGGAGTTTACCCGCTAATCACACTCAAAGAAGCACGCAGGCGCGCCACGGAGAGCAGATCCCTTATTGCCAATGGAATTAACCCAGTGGAACAAGCCCGCAAGGAAAAAGCCATTGATGCGCTCAACATGGCGGCAGGATTTAAGAAGGTGGCGGAAGACTGGTTTGCTACCAGGGTTGGAGGGTGGTCGGAATCCTATGCAAAACAGGTCAGATCGGC  >  CP009273/2552314‑2552608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑