Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 233,829 C→A 21.9% S3* (TCA→TAA)  yafT → lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273233,8290CA21.9% 633.8 / 126.0 256S3* (TCA→TAA) yafTlipoprotein
Reads supporting (aligned to +/- strand):  ref base C (107/93);  new base A (28/28);  total (135/121)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.13e-01

Only 100 of 259 total aligned reads displayed.

GAGAATAAATTTATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTT  >  CP009273/233682‑233976
                                                                                                                                                   |                                                                                                                                                   
  gaATAAATTTATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTaaa                                                                                                                                                   <  2:2374478/148‑1 (MQ=255)
   aaTAAATTTATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCaaa                                                                                                                                                  <  2:1561051/148‑1 (MQ=255)
     tAAATTTATATTGATTAACTGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCaaaaa                                                                                                                                                <  1:1896921/148‑1 (MQ=255)
      aaaTTTATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAaaaaaa                                                                                                                                               >  1:969911/1‑148 (MQ=255)
         tttATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCt                                                                                                                                            <  1:1415828/148‑1 (MQ=255)
         tttATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCt                                                                                                                                            <  1:1184772/148‑1 (MQ=255)
          ttATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCtt                                                                                                                                           >  2:1215091/1‑148 (MQ=255)
           tataTTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCttt                                                                                                                                          >  2:3482709/1‑148 (MQ=255)
                tGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGc                                                                                                                                     >  1:1256683/1‑148 (MQ=255)
                     aaaTGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATAtg                                                                                                                                <  1:2676785/148‑1 (MQ=255)
                     aaaTGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATAtg                                                                                                                                <  1:186798/148‑1 (MQ=255)
                      aatgaatgTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATAtgt                                                                                                                               <  1:1070876/148‑1 (MQ=255)
                        tgaatgTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATAtgtgt                                                                                                                             <  1:3350925/148‑1 (MQ=255)
                          aatgTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATgtgtgt                                                                                                                           <  1:3536830/148‑1 (MQ=255)
                           atgTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGtt                                                                                                                          <  1:480135/148‑1 (MQ=255)
                           atgTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGtt                                                                                                                          <  1:3065399/148‑1 (MQ=255)
                           atgTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGtt                                                                                                                          <  1:2199779/148‑1 (MQ=255)
                            tgTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTa                                                                                                                         <  1:2384135/148‑1 (MQ=255)
                              tataTTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTAtt                                                                                                                       <  2:1155609/148‑1 (MQ=255)
                                tatTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATtct                                                                                                                     >  2:2771930/1‑148 (MQ=255)
                                tatTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATtct                                                                                                                     <  1:1064076/148‑1 (MQ=255)
                                 atTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATtctc                                                                                                                    <  1:2784683/148‑1 (MQ=255)
                                 atTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATtctc                                                                                                                    >  2:2394682/1‑148 (MQ=255)
                                   ttCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATTTGTGTTATTCTCgc                                                                                                                  >  2:54146/1‑148 (MQ=255)
                                   ttCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCgc                                                                                                                  <  2:2777010/148‑1 (MQ=255)
                                     cAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCgctg                                                                                                                >  1:262493/1‑148 (MQ=255)
                                        aTTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCtt                                                                                                             >  1:361036/1‑148 (MQ=255)
                                          tGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCtttc                                                                                                           >  2:2186654/1‑146 (MQ=255)
                                          tGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGc                                                                                                           >  2:37758/1‑148 (MQ=255)
                                             ttttGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAgg                                                                                                        <  1:3313359/148‑1 (MQ=255)
                                              tttgtttgTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGa                                                                                                       <  2:1573822/148‑1 (MQ=255)
                                                  tttgttATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGAtgtg                                                                                                   >  2:1715373/1‑148 (MQ=255)
                                                  tttgttATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGAtgtg                                                                                                   >  1:2829472/1‑148 (MQ=255)
                                                     gttATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCct                                                                                                <  1:2875748/148‑1 (MQ=255)
                                                     gttATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCct                                                                                                >  1:2611287/1‑148 (MQ=255)
                                                        attattTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTg                                                                                             <  2:453147/148‑1 (MQ=255)
                                                         ttattTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGa                                                                                            >  1:2668256/1‑148 (MQ=255)
                                                          tattTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGaa                                                                                           >  1:1996262/1‑148 (MQ=255)
                                                            tttAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAAtc                                                                                         >  2:3532752/1‑148 (MQ=255)
                                                              tAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAAtctt                                                                                       <  2:582980/148‑1 (MQ=255)
                                                                 gTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTa                                                                                    >  1:1834377/1‑148 (MQ=255)
                                                                  tGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTAt                                                                                   >  1:811545/1‑148 (MQ=255)
                                                                     ggTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATtga                                                                                <  1:3001589/148‑1 (MQ=255)
                                                                     ggTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATtga                                                                                <  1:2347177/148‑1 (MQ=255)
                                                                     ggTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCGGAATCTTCTATtga                                                                                >  1:2155682/1‑148 (MQ=255)
                                                                        atataATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATtgatga                                                                             <  2:1724418/148‑1 (MQ=255)
                                                                         tataATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGaa                                                                            <  1:3343402/148‑1 (MQ=255)
                                                                          ataATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGaaa                                                                           >  1:1691870/1‑148 (MQ=255)
                                                                           taATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGaaaa                                                                          <  1:2656186/148‑1 (MQ=255)
                                                                            aaTTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAaag                                                                         >  2:3469980/1‑148 (MQ=255)
                                                                            aaTTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGaaatg                                                                         >  2:2957595/1‑146 (MQ=255)
                                                                              ttAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAaagaa                                                                       >  1:10387/1‑148 (MQ=255)
                                                                              ttAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAaagaa                                                                       <  2:1274353/148‑1 (MQ=255)
                                                                               tAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAaagaag                                                                      <  1:510070/148‑1 (MQ=255)
                                                                               tAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAaagaag                                                                      >  2:2903946/1‑148 (MQ=255)
                                                                               tAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAaagaag                                                                      >  2:2087988/1‑148 (MQ=255)
                                                                               tAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAaagaag                                                                      >  2:20049/1‑148 (MQ=255)
                                                                                  agTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGaaa                                                                   >  1:3559380/1‑148 (MQ=255)
                                                                                  agTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGaaa                                                                   >  2:1050911/1‑148 (MQ=255)
                                                                                    tCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGaaaaa                                                                 >  1:74569/1‑148 (MQ=255)
                                                                                     ccGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGaaaaaa                                                                <  1:996282/148‑1 (MQ=255)
                                                                                      cGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAg                                                               >  1:2953234/1‑148 (MQ=255)
                                                                                       gTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGc                                                              >  1:574535/1‑148 (MQ=255)
                                                                                        ttATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCt                                                             >  1:2090847/1‑148 (MQ=255)
                                                                                          aTCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCa                                                           >  2:2986049/1‑148 (MQ=255)
                                                                                            cAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAg                                                         >  1:1140149/1‑148 (MQ=255)
                                                                                            cAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAg                                                         >  1:1295812/1‑148 (MQ=255)
                                                                                             aaTGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGt                                                        <  1:2771549/148‑1 (MQ=255)
                                                                                              aTGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTc                                                       <  2:2700188/148‑1 (MQ=255)
                                                                                              aTGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTc                                                       >  1:669808/1‑148 (MQ=255)
                                                                                               tGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTca                                                      >  1:2146734/1‑148 (MQ=255)
                                                                                                gCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTcac                                                     >  1:1502925/1‑148 (MQ=255)
                                                                                                        ttCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTa                                             >  1:2424610/1‑148 (MQ=255)
                                                                                                         tCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTaa                                            >  1:1612935/1‑148 (MQ=255)
                                                                                                         tCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTaa                                            >  1:835033/1‑148 (MQ=255)
                                                                                                          cTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAat                                           <  2:2256680/148‑1 (MQ=255)
                                                                                                            aaTCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAatat                                         >  1:3102567/1‑148 (MQ=255)
                                                                                                              tCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATta                                       <  1:2506158/148‑1 (MQ=255)
                                                                                                              tCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATta                                       <  2:3255465/148‑1 (MQ=255)
                                                                                                              tCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATta                                       >  2:464126/1‑148 (MQ=255)
                                                                                                               cATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATtaa                                      >  2:1682779/1‑148 (MQ=255)
                                                                                                                    tGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATaaaa                                 <  1:3218986/148‑1 (MQ=255)
                                                                                                                     gACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATaaaaa                                >  1:1729786/1‑148 (MQ=255)
                                                                                                                        aGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAacac                             <  1:1288315/148‑1 (MQ=255)
                                                                                                                           cGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTcc                          >  2:193740/1‑148 (MQ=255)
                                                                                                                            gAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTccc                         <  2:2829472/148‑1 (MQ=255)
                                                                                                                             aGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTcccc                        >  2:2766600/1‑148 (MQ=255)
                                                                                                                              gggAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCa                       >  1:1735763/1‑148 (MQ=255)
                                                                                                                                  gtgtCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCaa                   >  2:2657171/1‑148 (MQ=255)
                                                                                                                                  gtgtCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCaa                   <  2:1442756/148‑1 (MQ=255)
                                                                                                                                     tCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTg                <  1:37758/148‑1 (MQ=255)
                                                                                                                                     tCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTg                >  1:3453520/1‑148 (MQ=255)
                                                                                                                                      ccAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTga               >  2:2466290/1‑148 (MQ=255)
                                                                                                                                       cAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTgac              <  1:1214858/148‑1 (MQ=255)
                                                                                                                                        aaTTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTgaca             <  2:3380588/148‑1 (MQ=255)
                                                                                                                                        aaTTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTgaca             <  2:1612935/148‑1 (MQ=255)
                                                                                                                                          ttATGAATTCAAAAAAGATTTGTTGCATATGTGTGTTATTCTCTCTGCTTGCAGTATGTGCATCTGAAACTTCTATTGATGAAAATAAGAAAAAAGCTCAAGACACACAAATTAATATTAATAAAAACACTACCAACCAACTgacaga           >  1:2034330/1‑148 (MQ=255)
                                                                                                                                              gAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACaaa       >  2:683304/1‑148 (MQ=255)
                                                                                                                                                  tCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGAtt   <  2:1256683/148‑1 (MQ=255)
                                                                                                                                                   cAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTATGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGAttt  <  1:1715373/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GAGAATAAATTTATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTT  >  CP009273/233682‑233976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑