Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,567,211 C→A 100% D152Y (GAT→TAT)  eutT ← cobalamin adenosyltransferase involved in ethanolamine utilization

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,567,2110CA96.5% 500.7 / 9.2 170D152Y (GAT→TAT) eutTcobalamin adenosyltransferase involved in ethanolamine utilization
Reads supporting (aligned to +/- strand):  ref base C (4/2);  new base A (72/92);  total (76/94)
Fisher's exact test for biased strand distribution p-value = 4.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 170 total aligned reads displayed.

CAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTG  >  CP009273/2567068‑2567353
                                                                                                                                               |                                                                                                                                              
     cagcaTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCAt                                                                                                                                       >  1:307499/1‑148 (MQ=255)
      agcaTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATa                                                                                                                                      <  1:2041546/148‑1 (MQ=255)
         aTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATg                                                                                                                                   <  2:314228/148‑1 (MQ=255)
         aTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATg                                                                                                                                   <  1:2621303/148‑1 (MQ=255)
          tCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGt                                                                                                                                  >  2:1284643/1‑148 (MQ=255)
           cgcgCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGtt                                                                                                                                 >  1:447182/1‑148 (MQ=255)
                  gTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGa                                                                                                                          <  1:1239150/148‑1 (MQ=255)
                    ggctggctTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGAcg                                                                                                                        <  2:2490013/148‑1 (MQ=255)
                      ctggctTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGAcgcg                                                                                                                      >  2:366798/1‑148 (MQ=255)
                       tggctTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGa                                                                                                                     >  2:2214769/1‑148 (MQ=255)
                       tggctTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGa                                                                                                                     <  2:195778/148‑1 (MQ=255)
                         gctTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAAc                                                                                                                   <  1:3333056/148‑1 (MQ=255)
                         gctTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAAc                                                                                                                   <  1:2346953/148‑1 (MQ=255)
                            tCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGa                                                                                                                >  1:1343107/1‑148 (MQ=255)
                            tCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGa                                                                                                                >  2:3375474/1‑148 (MQ=255)
                            tCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGa                                                                                                                >  2:683514/1‑148 (MQ=255)
                               ggCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGatat                                                                                                             <  1:130852/148‑1 (MQ=255)
                                   ccAGATGATCGTGGTCGAGATAGCGCCGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCgc                                                                                                         <  1:2563332/148‑1 (MQ=255)
                                          atCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCNGGCAGCCGAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCAc                                                                                                  >  1:1811120/1‑148 (MQ=255)
                                             gTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGc                                                                                               <  1:876855/148‑1 (MQ=255)
                                             gTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGc                                                                                               <  1:2225969/148‑1 (MQ=255)
                                               ggTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTg                                                                                             <  2:1990426/148‑1 (MQ=255)
                                                gTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGc                                                                                            >  1:3091077/1‑148 (MQ=255)
                                                gTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGc                                                                                            <  1:2617315/148‑1 (MQ=255)
                                                gTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGc                                                                                            <  1:2617308/148‑1 (MQ=255)
                                                 tCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGcc                                                                                           <  1:2170217/148‑1 (MQ=255)
                                                 tCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGcc                                                                                           >  1:3234891/1‑148 (MQ=255)
                                                    agaTAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACg                                                                                        <  1:1695619/148‑1 (MQ=255)
                                                      aTAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGt                                                                                      >  2:2582132/1‑148 (MQ=255)
                                                      aTAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGt                                                                                      >  2:3103803/1‑148 (MQ=255)
                                                         gcgcAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTcc                                                                                   >  2:1459609/1‑148 (MQ=255)
                                                         gcgcAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTcc                                                                                   >  2:1459571/1‑148 (MQ=255)
                                                          cgcAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCg                                                                                  >  2:1704436/1‑148 (MQ=255)
                                                          cgcAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCg                                                                                  >  1:831808/1‑148 (MQ=255)
                                                          cgcAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCg                                                                                  >  1:832363/1‑148 (MQ=255)
                                                              gCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAg                                                                              >  1:1204808/1‑148 (MQ=255)
                                                                  cTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCa                                                                          >  2:2060052/1‑148 (MQ=255)
                                                                  cTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCa                                                                          >  2:3140023/1‑148 (MQ=255)
                                                                   tgatgaGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCaa                                                                         <  1:3009185/148‑1 (MQ=255)
                                                                     atgaGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAAtt                                                                       >  2:661198/1‑148 (MQ=255)
                                                                     atgaGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAAtt                                                                       <  2:1113792/148‑1 (MQ=255)
                                                                     atgaGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCANAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAAtt                                                                       >  1:1805838/1‑148 (MQ=255)
                                                                       gagaAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCATCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCAAAGACTCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGATCAATTTg                                                                     >  1:2025888/1‑148 (MQ=255)
                                                                       gagaAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTg                                                                     >  1:2876991/1‑148 (MQ=255)
                                                                        agaAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGc                                                                    <  2:178919/148‑1 (MQ=255)
                                                                        agaAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGc                                                                    <  1:1750179/148‑1 (MQ=255)
                                                                        agaAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGc                                                                    >  2:400636/1‑148 (MQ=255)
                                                                         gaAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCCGTTCAATTTGCa                                                                   >  2:3521598/1‑148 (MQ=255)
                                                                          aaaGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAg                                                                  >  2:1504724/1‑148 (MQ=255)
                                                                          aaaGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAg                                                                  >  2:1504698/1‑148 (MQ=255)
                                                                             gCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCca                                                               >  1:1548328/1‑148 (MQ=255)
                                                                             gCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCca                                                               >  2:1731196/1‑148 (MQ=255)
                                                                              ccGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCcac                                                              <  1:1768522/148‑1 (MQ=255)
                                                                                gATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCacac                                                            >  2:3093725/1‑148 (MQ=255)
                                                                                   gCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGc                                                         <  2:2497421/148‑1 (MQ=255)
                                                                                   gCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGc                                                         >  1:1059326/1‑148 (MQ=255)
                                                                                      gATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAg                                                      <  1:3202025/148‑1 (MQ=255)
                                                                                       aTCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAgc                                                     >  1:3504557/1‑148 (MQ=255)
                                                                                        tCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAgcg                                                    >  2:2782963/1‑148 (MQ=255)
                                                                                          ttCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCa                                                  <  1:2241685/148‑1 (MQ=255)
                                                                                          ttCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCa                                                  <  2:3351391/148‑1 (MQ=255)
                                                                                           tCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCaa                                                 <  1:1785941/148‑1 (MQ=255)
                                                                                           tCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCaa                                                 <  1:3415395/148‑1 (MQ=255)
                                                                                               cAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGt                                             >  2:708762/1‑148 (MQ=255)
                                                                                               cAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGt                                             >  2:1391307/1‑148 (MQ=255)
                                                                                                aGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTg                                            <  1:903826/148‑1 (MQ=255)
                                                                                                 gAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGc                                           <  2:3206964/148‑1 (MQ=255)
                                                                                                   aaGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTg                                         <  1:2211296/148‑1 (MQ=255)
                                                                                                   aaGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTg                                         >  2:2934599/1‑148 (MQ=255)
                                                                                                    aGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGt                                        >  1:8553/1‑148 (MQ=255)
                                                                                                     gCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTc                                       >  1:1346641/1‑148 (MQ=255)
                                                                                                         gACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCgaga                                   <  1:429316/148‑1 (MQ=255)
                                                                                                          aCAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGaa                                  <  1:1071896/148‑1 (MQ=255)
                                                                                                          aCAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGaa                                  <  1:2781037/148‑1 (MQ=255)
                                                                                                              tCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACcgc                              >  2:1107323/1‑148 (MQ=255)
                                                                                                              tCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACcgc                              >  1:2995366/1‑148 (MQ=255)
                                                                                                               cGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACcgcg                             >  1:2337374/1‑148 (MQ=255)
                                                                                                                 gcTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACcgcgcg                           >  2:1713008/2‑148 (MQ=255)
                                                                                                                    ggCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGaaa                        <  2:1023519/148‑1 (MQ=255)
                                                                                                                     gCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGaaaa                       <  1:1930881/148‑1 (MQ=255)
                                                                                                                       aGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAAcc                     <  1:1083725/148‑1 (MQ=255)
                                                                                                                       aGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAAcc                     <  1:1523011/148‑1 (MQ=255)
                                                                                                                       aGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAAcc                     <  1:2928550/148‑1 (MQ=255)
                                                                                                                           cAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGa                 <  1:2341919/148‑1 (MQ=255)
                                                                                                                           cAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGa                 <  1:2341853/148‑1 (MQ=255)
                                                                                                                               ggTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGAcgcg             <  1:2353623/148‑1 (MQ=255)
                                                                                                                                gTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCgg            >  2:322606/1‑148 (MQ=255)
                                                                                                                                 ttCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGa           <  1:814250/148‑1 (MQ=255)
                                                                                                                                 ttCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGa           <  1:814462/148‑1 (MQ=255)
                                                                                                                                  tCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGAt          >  1:253181/1‑148 (MQ=255)
                                                                                                                                    ccccAGCGCATAGGCGCGCATAATGTTGCCAAGAGGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCg        <  2:2823138/148‑1 (MQ=255)
                                                                                                                                     cccAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGc       >  2:3504799/1‑148 (MQ=255)
                                                                                                                                     cccAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGc       <  2:2680484/148‑1 (MQ=255)
                                                                                                                                     cccAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGc       <  2:2642427/148‑1 (MQ=255)
                                                                                                                                       cAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGTACGGATATCCGCCAGCCACGGCTGCCACGGTTGCGCCAGTTCAATTTGCAGCCTCACGGCCAGCGCAATGGTGCTGTCGAGAACCGCTCGAAAACCCAGACGCGGATCGCtt     <  2:292679/148‑1 (MQ=255)
                                                                                                                                       cAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCtt     <  2:447182/148‑1 (MQ=255)
                                                                                                                                        aGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCttt    <  1:368798/148‑1 (MQ=255)
                                                                                                                                        aGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCttt    <  2:1343107/148‑1 (MQ=255)
                                                                                                                                          cgcATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTg  <  2:831797/148‑1 (MQ=255)
                                                                                                                                          cgcATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTg  <  2:832363/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                              
CAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTG  >  CP009273/2567068‑2567353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑