Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,601,971 C→A 7.7% I42I (ATC→ATA)  hyfG → hydrogenase 4, subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,601,9710CA 7.7% 711.9 / 22.4 194I42I (ATC→ATA) hyfGhydrogenase 4, subunit
Reads supporting (aligned to +/- strand):  ref base C (73/106);  new base A (6/9);  total (79/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 208 total aligned reads displayed.

GCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATT  >  CP009273/2601824‑2602118
                                                                                                                                                   |                                                                                                                                                   
gCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAaatc                                                                                                                                                     >  2:827778/1‑148 (MQ=255)
 cATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAaatca                                                                                                                                                    <  1:1652961/148‑1 (MQ=255)
  aTCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAaatcaa                                                                                                                                                   <  1:3246878/148‑1 (MQ=255)
    cTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAaatcaatc                                                                                                                                                 >  2:1918203/1‑148 (MQ=255)
    cTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAaataaatc                                                                                                                                                 >  2:2773694/1‑148 (MQ=255)
       tttCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCACTctgc                                                                                                                                              >  1:2583600/1‑148 (MQ=255)
       tttCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATctgc                                                                                                                                              <  1:2778041/148‑1 (MQ=255)
       tttCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATctgc                                                                                                                                              >  1:229780/1‑148 (MQ=255)
       tttCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATctgc                                                                                                                                              >  1:229554/1‑148 (MQ=255)
        ttCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATctgct                                                                                                                                             >  1:713837/1‑148 (MQ=255)
          cGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCtgctgc                                                                                                                                           <  2:1891431/148‑1 (MQ=255)
            gagaAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCt                                                                                                                                         >  2:3103497/1‑148 (MQ=255)
            gagaAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCt                                                                                                                                         <  1:3447611/148‑1 (MQ=255)
            gagaAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCt                                                                                                                                         >  1:510121/1‑148 (MQ=255)
             agaAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTg                                                                                                                                        >  2:860160/1‑148 (MQ=255)
              gaAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGa                                                                                                                                       <  1:2235852/148‑1 (MQ=255)
               aaaCACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGAc                                                                                                                                      >  1:2598364/1‑148 (MQ=255)
                aaCACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACg                                                                                                                                     <  1:986993/148‑1 (MQ=255)
                 acacAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGt                                                                                                                                    >  1:1295600/1‑148 (MQ=255)
                 acacAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGt                                                                                                                                    >  1:3519828/1‑148 (MQ=255)
                 acacAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGt                                                                                                                                    >  1:2991646/1‑148 (MQ=255)
                    caGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGt                                                                                                                                 <  2:1947766/148‑1 (MQ=255)
                        gAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAg                                                                                                                             >  1:1002331/1‑148 (MQ=255)
                        gAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAg                                                                                                                             >  2:1577591/1‑148 (MQ=255)
                        gAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAg                                                                                                                             >  2:520129/1‑148 (MQ=255)
                         aaCGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGt                                                                                                                            <  2:2474138/148‑1 (MQ=255)
                         aaCGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGt                                                                                                                            >  1:2868084/1‑148 (MQ=255)
                          aCGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTa                                                                                                                           <  2:920721/148‑1 (MQ=255)
                           cGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTAt                                                                                                                          >  1:1752934/1‑148 (MQ=255)
                               aagtCATCGTCAAATCGGGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGTATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCttt                                                                                                                      <  1:2610079/145‑1 (MQ=255)
                                aTTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAGTAAATCTGCTGCCTGACGTTGTACAGTATCTtta                                                                                                                     >  2:2644994/1‑148 (MQ=255)
                                  tCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTttatt                                                                                                                   >  1:1051200/1‑148 (MQ=255)
                                    aTCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATAAATCTGCTGCCTGACGTTGTACAGTATCTTtattat                                                                                                                 <  2:2884045/148‑1 (MQ=255)
                                     tCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATc                                                                                                                <  1:2435360/148‑1 (MQ=255)
                                      cGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCa                                                                                                               >  2:495137/1‑148 (MQ=255)
                                        tCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAAc                                                                                                             <  1:1152223/148‑1 (MQ=255)
                                         cAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACa                                                                                                            >  2:1973640/1‑148 (MQ=255)
                                           aaTCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACatg                                                                                                          >  1:2537207/1‑148 (MQ=255)
                                           aaTCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACatg                                                                                                          <  1:2270459/148‑1 (MQ=255)
                                            aTCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACatga                                                                                                         <  1:1523231/148‑1 (MQ=255)
                                            aTCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACatga                                                                                                         <  1:481600/148‑1 (MQ=255)
                                                     aaGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATggctggct                                                                                                <  2:613289/148‑1 (MQ=255)
                                                      aGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCtt                                                                                               <  1:466299/148‑1 (MQ=255)
                                                       gCGATCCCCGCCGCCCTGAACCCGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTc                                                                                              <  2:3293316/148‑1 (MQ=255)
                                                         gATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCg                                                                                            <  2:2842971/148‑1 (MQ=255)
                                                           ttCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGt                                                                                          >  1:1175327/1‑148 (MQ=255)
                                                             ctcGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTcc                                                                                        <  2:1095923/148‑1 (MQ=255)
                                                              tcGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCt                                                                                       <  1:827778/148‑1 (MQ=255)
                                                               cgccgcCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTg                                                                                      <  1:3496935/148‑1 (MQ=255)
                                                               cgccgcCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTg                                                                                      <  1:1575642/148‑1 (MQ=255)
                                                                 ccgccCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGtt                                                                                    >  2:1587636/1‑148 (MQ=255)
                                                                   gccCTGAAAACGCAGGTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTg                                                                                  <  2:1998152/148‑1 (MQ=255)
                                                                        gAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATAAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGTCTGGCTTCCGGTCCTGTTTGGCAac                                                                             >  1:3336090/1‑148 (MQ=255)
                                                                           aaCGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATAAATCTGCTGCCTGACGGTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAacgac                                                                          >  2:2079637/1‑148 (MQ=255)
                                                                              gCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAg                                                                       <  2:1295600/148‑1 (MQ=255)
                                                                                    ccccGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGaca                                                                 >  2:10342/1‑148 (MQ=255)
                                                                                     cccGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGacac                                                                <  1:589878/148‑1 (MQ=255)
                                                                                      ccGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACt                                                               <  2:2411665/148‑1 (MQ=255)
                                                                                      ccGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACt                                                               <  2:3021134/148‑1 (MQ=255)
                                                                                         gcgcGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTaa                                                            <  2:2742778/148‑1 (MQ=255)
                                                                                          cgcgGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAAc                                                           <  1:817225/148‑1 (MQ=255)
                                                                                               tGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCa                                                      <  1:2140194/148‑1 (MQ=255)
                                                                                               tGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCa                                                      <  1:844914/148‑1 (MQ=255)
                                                                                                gCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCAt                                                     <  2:3272680/148‑1 (MQ=255)
                                                                                                 cTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCAtt                                                    <  1:3291533/148‑1 (MQ=255)
                                                                                                 cTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCAtt                                                    <  1:2620778/148‑1 (MQ=255)
                                                                                                  tGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTTCCTGACGTTGTACATTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTa                                                   <  2:660834/148‑1 (MQ=255)
                                                                                                  tGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTa                                                   <  1:3103497/148‑1 (MQ=255)
                                                                                                   ggATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTAc                                                  >  1:1833749/1‑148 (MQ=255)
                                                                                                         agagCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGtt                                            <  2:256687/148‑1 (MQ=255)
                                                                                                          gagCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGttt                                           <  2:229554/148‑1 (MQ=255)
                                                                                                          gagCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGttt                                           <  2:229780/148‑1 (MQ=255)
                                                                                                           agCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGGTGCCTGACGTTGTACAGTATCTTTTTTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTtta                                          <  2:3298824/148‑1 (MQ=255)
                                                                                                           agCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTtta                                          <  1:2697586/148‑1 (MQ=255)
                                                                                                               aCAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTtattat                                      >  1:2257716/1‑148 (MQ=255)
                                                                                                                 aaaCGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGc                                    <  1:1980796/148‑1 (MQ=255)
                                                                                                                  aaCGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGcc                                   <  2:3348537/148‑1 (MQ=255)
                                                                                                                  aaCGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGcc                                   <  2:1230616/148‑1 (MQ=255)
                                                                                                                       cTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTc                              >  1:2048250/1‑148 (MQ=255)
                                                                                                                        tGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCa                             <  2:1650900/148‑1 (MQ=255)
                                                                                                                        tGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCa                             <  2:1155436/148‑1 (MQ=255)
                                                                                                                         gAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCaa                            <  1:860160/148‑1 (MQ=255)
                                                                                                                         gAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCaa                            >  1:141693/1‑148 (MQ=255)
                                                                                                                         gAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCaa                            <  1:2341493/148‑1 (MQ=255)
                                                                                                                             aGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGa                        <  2:2537207/148‑1 (MQ=255)
                                                                                                                               gTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAg                      >  1:2176370/1‑148 (MQ=255)
                                                                                                                               gTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTGTATTATGCCCTTTCAATGGAAg                      >  1:382311/1‑148 (MQ=255)
                                                                                                                                tCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAgg                     <  2:2158833/148‑1 (MQ=255)
                                                                                                                                tCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGCATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAgg                     <  2:3442177/148‑1 (MQ=255)
                                                                                                                                 cACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAggg                    >  2:2362537/1‑148 (MQ=255)
                                                                                                                                   ccATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGc                  <  2:3519828/148‑1 (MQ=255)
                                                                                                                                     aTTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCg                >  2:3408204/1‑148 (MQ=255)
                                                                                                                                         cGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGaaaa            <  1:3202808/148‑1 (MQ=255)
                                                                                                                                         cGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGACAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGaaaa            <  1:643318/148‑1 (MQ=255)
                                                                                                                                         cGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGACAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGaaaa            <  1:2430309/148‑1 (MQ=255)
                                                                                                                                          ggTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGaaaaa           <  2:1318354/148‑1 (MQ=255)
                                                                                                                                           gTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAAt          >  1:1585464/1‑148 (MQ=255)
                                                                                                                                            tGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATg         <  2:713837/148‑1 (MQ=255)
                                                                                                                                                  tcaatcTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGAt   <  1:3408204/148‑1 (MQ=255)
                                                                                                                                                   caatcTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGAtt  >  1:3285820/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATT  >  CP009273/2601824‑2602118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑