Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,659,594 C→A 100% L267L (CTC→CTA)  csiE → stationary phase inducible protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,659,5940CA96.5% 606.1 / 11.8 200L267L (CTC→CTA) csiEstationary phase inducible protein
Reads supporting (aligned to +/- strand):  ref base C (2/5);  new base A (111/82);  total (113/87)
Fisher's exact test for biased strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 200 total aligned reads displayed.

GGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTT  >  CP009273/2659450‑2659735
                                                                                                                                                |                                                                                                                                             
ggTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGcc                                                                                                                                            <  2:434946/148‑1 (MQ=255)
ggTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGcc                                                                                                                                            <  1:3425783/148‑1 (MQ=255)
 gTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGccc                                                                                                                                           <  1:2142039/148‑1 (MQ=255)
      cGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCg                                                                                                                                      <  2:1867200/148‑1 (MQ=255)
      cGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCg                                                                                                                                      >  1:2480604/1‑148 (MQ=255)
       gATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGt                                                                                                                                     <  2:3469108/148‑1 (MQ=255)
        aTCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGtt                                                                                                                                    >  1:1751569/1‑148 (MQ=255)
         tCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTa                                                                                                                                   >  1:2636417/1‑148 (MQ=255)
            aTTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAAt                                                                                                                                >  1:1373658/1‑148 (MQ=255)
            aTTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAAt                                                                                                                                >  1:3147631/1‑148 (MQ=255)
             ttGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTCTACCCACCTAGCCCAGGCGTTAAATc                                                                                                                               >  1:3532974/1‑148 (MQ=255)
              tGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCg                                                                                                                              <  1:1557039/148‑1 (MQ=255)
                  ggATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgt                                                                                                                          <  2:2103892/148‑1 (MQ=255)
                  ggATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgt                                                                                                                          >  1:2344981/1‑148 (MQ=255)
                   gATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgtt                                                                                                                         <  2:173374/148‑1 (MQ=255)
                      gcgcATCAGCGGGAGAGAGAATTTCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgtttgt                                                                                                                      <  2:1249650/148‑1 (MQ=255)
                            cAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCa                                                                                                                >  1:104208/1‑148 (MQ=255)
                            cAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCa                                                                                                                >  2:917079/1‑148 (MQ=255)
                             aGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCAt                                                                                                               <  2:3418562/148‑1 (MQ=255)
                             aGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCAt                                                                                                               <  2:467256/148‑1 (MQ=255)
                               cGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCg                                                                                                             >  2:132377/1‑148 (MQ=255)
                                gggACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCgg                                                                                                            <  2:3228823/148‑1 (MQ=255)
                                  gacagacaATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTa                                                                                                          >  1:2564221/1‑148 (MQ=255)
                                   acagacaATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTAt                                                                                                         <  2:139884/148‑1 (MQ=255)
                                       acaATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGat                                                                                                     >  2:1783302/1‑148 (MQ=255)
                                        caATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTCTTGata                                                                                                    >  1:2692470/1‑148 (MQ=255)
                                         aaTTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGataa                                                                                                   <  1:782177/148‑1 (MQ=255)
                                          aTTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGataat                                                                                                  >  1:558439/1‑148 (MQ=255)
                                          aTTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGataat                                                                                                  <  2:1498498/148‑1 (MQ=255)
                                          aTTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGataat                                                                                                  >  1:2826776/1‑148 (MQ=255)
                                          aTTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGataat                                                                                                  >  2:923990/1‑148 (MQ=255)
                                           gtGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGataata                                                                                                 <  2:1379232/147‑1 (MQ=255)
                                            tGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATAc                                                                                                <  2:1322575/148‑1 (MQ=255)
                                            tGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATAc                                                                                                <  2:1322223/148‑1 (MQ=255)
                                            tGCGGCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATAc                                                                                                <  2:710657/148‑1 (MQ=255)
                                              cGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATAccc                                                                                              >  2:2545957/1‑148 (MQ=255)
                                                        tCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAaga                                                                                    <  1:199576/148‑1 (MQ=255)
                                                        tCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAaga                                                                                    >  1:3495646/1‑148 (MQ=255)
                                                         cAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAagag                                                                                   <  1:3029080/148‑1 (MQ=255)
                                                         cAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAagag                                                                                   <  1:2191754/148‑1 (MQ=255)
                                                           aaCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGtt                                                                                 >  1:1220662/1‑148 (MQ=255)
                                                           aaCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGtt                                                                                 >  2:2164525/1‑148 (MQ=255)
                                                            aCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTc                                                                                >  2:1774264/1‑148 (MQ=255)
                                                               tCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGcc                                                                             >  2:2065914/1‑148 (MQ=255)
                                                                  gggaaaCCATTTTCGGGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGa                                                                          <  2:440599/145‑1 (MQ=255)
                                                                   gTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGAc                                                                         >  2:617834/1‑148 (MQ=255)
                                                                    tAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACt                                                                        >  2:928125/1‑148 (MQ=255)
                                                                     aaaCCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTg                                                                       <  1:3249694/148‑1 (MQ=255)
                                                                      aaCCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTTCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGt                                                                      <  1:2594406/148‑1 (MQ=255)
                                                                      aaCCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGt                                                                      <  2:792978/148‑1 (MQ=255)
                                                                       aCCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTa                                                                     >  2:2949506/1‑148 (MQ=255)
                                                                       aCCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTa                                                                     <  2:1373658/148‑1 (MQ=255)
                                                                       aCCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTa                                                                     <  2:3147631/148‑1 (MQ=255)
                                                                         cATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTAcc                                                                   >  1:556067/1‑148 (MQ=255)
                                                                         cATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTAcc                                                                   >  2:1270939/1‑148 (MQ=255)
                                                                         cATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTAcc                                                                   >  2:3041575/1‑148 (MQ=255)
                                                                              tCGTGAGCTGTGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGc                                                              <  2:3440218/148‑1 (MQ=255)
                                                                                   aGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGt                                                         >  2:1820739/1‑148 (MQ=255)
                                                                                   aGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGATTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGt                                                         >  2:2192101/1‑148 (MQ=255)
                                                                                       gggAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTgcgc                                                     >  2:612465/1‑148 (MQ=255)
                                                                                       gggAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTgcgc                                                     >  1:483227/1‑148 (MQ=255)
                                                                                       gggAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTgcgc                                                     >  1:1489764/1‑148 (MQ=255)
                                                                                          aaaTGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGcacc                                                  >  2:1189640/1‑148 (MQ=255)
                                                                                           aaTGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGcacca                                                 >  1:90906/1‑148 (MQ=255)
                                                                                           aaTGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGcacca                                                 >  1:432446/1‑148 (MQ=255)
                                                                                            aTGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATAACCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGcaccac                                                >  1:3308018/1‑148 (MQ=255)
                                                                                             tGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCaccacc                                               >  2:3374259/1‑148 (MQ=255)
                                                                                               ttCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAATAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCcg                                             >  1:2839567/1‑148 (MQ=255)
                                                                                               ttCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCcg                                             >  1:1399277/1‑148 (MQ=255)
                                                                                                tCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCcgc                                            >  1:2713945/1‑148 (MQ=255)
                                                                                                tCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCcgc                                            >  2:2810812/1‑148 (MQ=255)
                                                                                                          gatgaACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTgg                                  <  2:3274509/148‑1 (MQ=255)
                                                                                                           atgaACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGNATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGc                                 >  1:1809748/1‑148 (MQ=255)
                                                                                                            tgaACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGcc                                >  2:1066474/1‑148 (MQ=255)
                                                                                                              aaCAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCgg                              <  2:368008/148‑1 (MQ=255)
                                                                                                              aaCAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCgg                              <  1:633969/148‑1 (MQ=255)
                                                                                                              aaCAGGGGTTATGTGATCAGCTTTAGACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCgg                              <  2:2162600/148‑1 (MQ=255)
                                                                                                               aCAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGa                             <  1:1656754/148‑1 (MQ=255)
                                                                                                                cAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGAt                            <  2:1585676/148‑1 (MQ=255)
                                                                                                                cAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGAt                            <  2:711593/148‑1 (MQ=255)
                                                                                                                 aGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGAtt                           >  1:3091515/1‑148 (MQ=255)
                                                                                                                  ggggTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGAttt                          <  1:3140498/148‑1 (MQ=255)
                                                                                                                   gggTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTg                         >  1:1844765/1‑148 (MQ=255)
                                                                                                                    ggTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGa                        >  2:2086085/1‑148 (MQ=255)
                                                                                                                        aTGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGt                    >  1:1096942/1‑148 (MQ=255)
                                                                                                                        aTGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGt                    <  1:2441493/148‑1 (MQ=255)
                                                                                                                        aTGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGt                    >  1:1098268/1‑148 (MQ=255)
                                                                                                                         tgtgATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTg                   >  1:3042557/1‑148 (MQ=255)
                                                                                                                          gtgATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGa                  <  1:2071101/148‑1 (MQ=255)
                                                                                                                           tgATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGaa                 >  2:516344/1‑148 (MQ=255)
                                                                                                                              tCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATAc              >  2:2391976/1‑148 (MQ=255)
                                                                                                                               cAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACg             >  1:814293/1‑148 (MQ=255)
                                                                                                                                  cTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCg          >  1:3180418/1‑148 (MQ=255)
                                                                                                                                  cTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCg          >  1:1620511/1‑148 (MQ=255)
                                                                                                                                   tttATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGt         <  1:1223622/148‑1 (MQ=255)
                                                                                                                                      ataCCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCa      <  1:2949506/148‑1 (MQ=255)
                                                                                                                                        aCCCACCTAGCCCAGGCTTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCCCCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATc    <  1:1478394/148‑1 (MQ=255)
                                                                                                                                        aCCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATc    >  1:3404171/1‑148 (MQ=255)
                                                                                                                                          ccACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCtt  >  1:732560/1‑148 (MQ=255)
                                                                                                                                             ccTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAAt                <  1:2105495/131‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                             
GGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTT  >  CP009273/2659450‑2659735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑