Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,668,397 C→A 65.4% G242C (GGT→TGT)  yphC ← putative Zn‑dependent NAD(P)‑binding oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,668,3970CA65.4% 117.5 / 209.0 182G242C (GGT→TGT) yphCputative Zn‑dependent NAD(P)‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (31/32);  new base A (61/58);  total (92/90)
Fisher's exact test for biased strand distribution p-value = 8.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 189 total aligned reads displayed.

GATGGTGCATCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCGCCA  >  CP009273/2668250‑2668543
                                                                                                                                                   |                                                                                                                                                  
gATGGTGCATCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCAcc                                                                                                                                                    >  1:2138935/1‑148 (MQ=255)
 aTGGTGCATCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCg                                                                                                                                                   >  1:2358455/1‑148 (MQ=255)
 aTGGTGCATCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCg                                                                                                                                                   <  1:50710/148‑1 (MQ=255)
  tGGTGCATCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGt                                                                                                                                                  <  1:2805256/148‑1 (MQ=255)
     tGCATCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTggg                                                                                                                                               <  2:1094247/148‑1 (MQ=255)
      gCATCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGt                                                                                                                                              >  1:2448109/1‑148 (MQ=255)
      gCATCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTCTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGt                                                                                                                                              >  1:2832547/1‑148 (MQ=255)
         tCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGTGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAg                                                                                                                                           <  2:3278731/148‑1 (MQ=255)
         tCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAg                                                                                                                                           >  1:1187090/1‑148 (MQ=255)
         tCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAg                                                                                                                                           >  1:594781/1‑148 (MQ=255)
         tCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAg                                                                                                                                           >  2:3520462/1‑148 (MQ=255)
         tCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAg                                                                                                                                           >  2:1880069/1‑148 (MQ=255)
         tCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACAGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAg                                                                                                                                           >  2:1970837/1‑148 (MQ=255)
          cAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGt                                                                                                                                          >  1:1145124/1‑148 (MQ=255)
           aGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGtt                                                                                                                                         <  2:1366934/148‑1 (MQ=255)
            gATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTc                                                                                                                                        >  1:89534/1‑148 (MQ=255)
             aTCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCg                                                                                                                                       >  2:1529191/1‑148 (MQ=255)
                ggCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCg                                                                                                                                    <  1:1731035/148‑1 (MQ=255)
                    cTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATaa                                                                                                                                <  1:413151/148‑1 (MQ=255)
                    cTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATaa                                                                                                                                >  2:2587448/1‑148 (MQ=255)
                      gACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATc                                                                                                                              <  1:2736473/148‑1 (MQ=255)
                          tCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCg                                                                                                                          >  2:1112989/1‑148 (MQ=255)
                          tCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCg                                                                                                                          >  1:1756910/1‑148 (MQ=255)
                           cGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCgg                                                                                                                         <  1:1650996/148‑1 (MQ=255)
                             aaTTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCa                                                                                                                       >  1:3117817/1‑148 (MQ=255)
                              aTTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAg                                                                                                                      >  1:1015145/1‑148 (MQ=255)
                               ttCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGa                                                                                                                     >  1:828079/1‑148 (MQ=255)
                               ttCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGa                                                                                                                     <  2:2304175/148‑1 (MQ=255)
                                tCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGAc                                                                                                                    >  1:2124539/1‑148 (MQ=255)
                                   aCTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCtt                                                                                                                 <  1:1206679/148‑1 (MQ=255)
                                   aCTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCtt                                                                                                                 >  2:1757930/1‑148 (MQ=255)
                                     ttttCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCg                                                                                                               <  2:3091467/148‑1 (MQ=255)
                                     ttttCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCg                                                                                                               >  2:156598/1‑148 (MQ=255)
                                         ccGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCggtgg                                                                                                           <  2:1667656/148‑1 (MQ=255)
                                         ccGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCggtgg                                                                                                           >  1:461406/1‑148 (MQ=255)
                                          cGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCggtggt                                                                                                          >  1:1379972/1‑148 (MQ=255)
                                          cGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCggtggt                                                                                                          >  1:1431234/1‑148 (MQ=255)
                                            gTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTg                                                                                                        <  2:1206468/148‑1 (MQ=255)
                                             tttCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGTCGATAATCTGCGGCAGACCTTCGGTGGTTGc                                                                                                       >  1:3040547/1‑148 (MQ=255)
                                                  ccAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAAt                                                                                                  <  1:3500310/148‑1 (MQ=255)
                                                   cAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCACCATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATa                                                                                                 >  1:1832711/1‑148 (MQ=255)
                                                     aTGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGc                                                                                               >  2:808703/1‑148 (MQ=255)
                                                           accaccCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGAt                                                                                         <  2:2891296/148‑1 (MQ=255)
                                                             cacgCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATcc                                                                                       <  2:2507500/148‑1 (MQ=255)
                                                             caccCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATcc                                                                                       <  1:367819/148‑1 (MQ=255)
                                                                ccGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATc                                                                                    >  1:412379/1‑148 (MQ=255)
                                                                 cGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCa                                                                                   >  2:213171/1‑148 (MQ=255)
                                                                 cGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCa                                                                                   >  2:1441550/1‑148 (MQ=255)
                                                                   tCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCAcc                                                                                 <  2:2388955/148‑1 (MQ=255)
                                                                   tCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCAcc                                                                                 <  2:2389092/148‑1 (MQ=255)
                                                                    ccccAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCAccc                                                                                <  1:123884/148‑1 (MQ=255)
                                                                     cccAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCAcccc                                                                               >  2:210096/1‑148 (MQ=255)
                                                                       cAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTa                                                                             >  1:2419033/1‑148 (MQ=255)
                                                                        aGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTaa                                                                            <  1:1214220/148‑1 (MQ=255)
                                                                           cAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTg                                                                         >  2:3514739/1‑148 (MQ=255)
                                                                           cAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTg                                                                         <  1:2326059/148‑1 (MQ=255)
                                                                            aGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGt                                                                        <  2:2703068/148‑1 (MQ=255)
                                                                             gCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGtt                                                                       <  1:2277097/148‑1 (MQ=255)
                                                                                  ggATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGcc                                                                  <  2:89534/148‑1 (MQ=255)
                                                                                  ggATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGcc                                                                  <  1:1028401/148‑1 (MQ=255)
                                                                                    aTTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCAt                                                                >  1:2135726/1‑148 (MQ=255)
                                                                                        cAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGcc                                                            <  2:465227/148‑1 (MQ=255)
                                                                                        cAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGcc                                                            <  2:2448577/148‑1 (MQ=255)
                                                                                             ccAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACg                                                       >  1:3190015/1‑148 (MQ=255)
                                                                                             ccAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACg                                                       >  1:3190043/1‑148 (MQ=255)
                                                                                             ccAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACg                                                       >  1:3190079/1‑148 (MQ=255)
                                                                                             ccAGCAAGCGACCTGCGGCATTACCGGAACAAACGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTATTGCCATCGCCAGACg                                                       >  1:2636813/1‑148 (MQ=255)
                                                                                               aGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGtt                                                     <  1:2484504/148‑1 (MQ=255)
                                                                                               aGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGtt                                                     <  1:143129/148‑1 (MQ=255)
                                                                                               aGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGtt                                                     <  1:808563/148‑1 (MQ=255)
                                                                                               aGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGtt                                                     >  2:3330103/1‑148 (MQ=255)
                                                                                                 cAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTcc                                                   >  2:3463575/1‑148 (MQ=255)
                                                                                                       aCCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGgcagc                                             >  2:1590009/1‑148 (MQ=255)
                                                                                                       aCCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGgcagc                                             >  2:3404350/1‑148 (MQ=255)
                                                                                                       aCCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGgcagc                                             >  2:2584977/1‑148 (MQ=255)
                                                                                                         cTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCat                                           <  2:2772696/148‑1 (MQ=255)
                                                                                                           gCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCatat                                         <  2:79318/148‑1 (MQ=255)
                                                                                                           gCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCatat                                         <  1:761738/148‑1 (MQ=255)
                                                                                                             ggCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCa                                       <  2:1187090/148‑1 (MQ=255)
                                                                                                             ggCATTACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCa                                       <  1:883210/148‑1 (MQ=255)
                                                                                                                aTTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACg                                    >  2:2824170/1‑148 (MQ=255)
                                                                                                                  tACCGGAACAATCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGcc                                  >  2:880198/1‑148 (MQ=255)
                                                                                                                        aaCAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCgatgat                            <  1:3514739/148‑1 (MQ=255)
                                                                                                                         aCAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATc                           >  1:2149962/1‑148 (MQ=255)
                                                                                                                          cAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATcc                          >  1:3383338/1‑148 (MQ=255)
                                                                                                                            aTCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGt                        >  2:1344625/1‑148 (MQ=255)
                                                                                                                            aTCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGt                        >  1:2604109/1‑148 (MQ=255)
                                                                                                                             tCGAGCGCAAGATCCGCTCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACATTAGGTGGTTGATAAATAGCAGTGATCCATCACACATAACTGTTTTGCCATCGCCAGACGTTACGGCAGCATATCAACGCCTATGATCCGtt                       <  2:2276713/148‑1 (MQ=255)
                                                                                                                             tCGAGCGCAACATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGtt                       <  1:970250/148‑1 (MQ=255)
                                                                                                                              cGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGttt                      >  2:302021/1‑148 (MQ=255)
                                                                                                                                  cgcAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTGTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCa                  <  2:2442943/148‑1 (MQ=255)
                                                                                                                                       cATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACcgcg             >  2:40005/1‑148 (MQ=255)
                                                                                                                                       cATCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACcgcg             <  2:594781/148‑1 (MQ=255)
                                                                                                                                         tCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGAc           >  1:1615132/1‑148 (MQ=255)
                                                                                                                                         tCCGCGCCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGAc           >  1:1090184/1‑148 (MQ=255)
                                                                                                                                           cgcgCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCt         >  1:394745/1‑148 (MQ=255)
                                                                                                                                            gcgcCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCtt        <  1:1973548/148‑1 (MQ=255)
                                                                                                                                             cgcCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCttt       <  2:3118085/148‑1 (MQ=255)
                                                                                                                                             cgcCACAGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCttt       <  1:2582044/148‑1 (MQ=255)
                                                                                                                                                  ccGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCGCCa  >  2:884062/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GATGGTGCATCAGATCGGCGCTGACCTCGAATTCCACTTTTCCGGTTTCACCAATGTAAACCACCCGTCCCCAGTCAGCGGTGGATTGCAGTGCCAGCAAGCGACCTGCGGCATTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCGCCA  >  CP009273/2668250‑2668543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑