Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,689,267 C→A 90.7% I36I (ATC→ATA)  mltF → membrane‑bound lytic transglycosylase F, murein hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,689,2670CA90.7% 467.0 / 37.3 179I36I (ATC→ATA) mltFmembrane‑bound lytic transglycosylase F, murein hydrolase
Reads supporting (aligned to +/- strand):  ref base C (10/7);  new base A (87/75);  total (97/82)
Fisher's exact test for biased strand distribution p-value = 8.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 184 total aligned reads displayed.

TTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGTG  >  CP009273/2689122‑2689411
                                                                                                                                                 |                                                                                                                                                
ttCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATagc                                                                                                                                                >  2:126573/1‑148 (MQ=255)
      cTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCAt                                                                                                                                          >  1:1862217/1‑148 (MQ=255)
         ggACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCa                                                                                                                                       >  2:976460/1‑148 (MQ=255)
         ggACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCa                                                                                                                                       >  1:651157/1‑148 (MQ=255)
         ggACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCa                                                                                                                                       >  1:1961795/1‑148 (MQ=255)
          gACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTATAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCaa                                                                                                                                      <  2:3258361/148‑1 (MQ=255)
          gACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCaa                                                                                                                                      >  2:310587/1‑148 (MQ=255)
                     cacaGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGgaga                                                                                                                           <  2:1018874/148‑1 (MQ=255)
                     cacaGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGgaga                                                                                                                           >  2:2980025/1‑148 (MQ=255)
                     cacaGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGgaga                                                                                                                           >  2:3098962/1‑148 (MQ=255)
                      acaGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGagag                                                                                                                          <  1:848200/148‑1 (MQ=255)
                      acaGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGagag                                                                                                                          <  2:2919442/148‑1 (MQ=255)
                      acaGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGagag                                                                                                                          <  2:701210/148‑1 (MQ=255)
                       caGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGt                                                                                                                         >  2:131763/1‑148 (MQ=255)
                       caGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGt                                                                                                                         >  2:1398781/1‑148 (MQ=255)
                        agagAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGtt                                                                                                                        >  1:163141/1‑148 (MQ=255)
                           gaATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCg                                                                                                                     >  1:608129/1‑148 (MQ=255)
                              ttAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCgtgt                                                                                                                  >  2:811902/1‑148 (MQ=255)
                                   tAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCa                                                                                                             >  1:3399411/1‑148 (MQ=255)
                                   tAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCAGTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCa                                                                                                             <  1:859041/148‑1 (MQ=255)
                                    aaTTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCAc                                                                                                            >  2:1658514/1‑148 (MQ=255)
                                     aTTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACCACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCAcc                                                                                                           >  1:3321464/1‑148 (MQ=255)
                                     aTTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGGGAGTTGCGTGGGAGCAcc                                                                                                           >  1:73261/1‑148 (MQ=255)
                                     aTTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGTGCAcc                                                                                                           >  1:518573/1‑148 (MQ=255)
                                      ttGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCCTTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCa                                                                                                          >  2:3324965/1‑148 (MQ=255)
                                      ttGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCa                                                                                                          <  1:3478359/148‑1 (MQ=255)
                                      ttGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCa                                                                                                          >  2:3563430/1‑148 (MQ=255)
                                      ttGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCa                                                                                                          >  2:362542/1‑148 (MQ=255)
                                      ttGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGCCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCa                                                                                                          <  1:3478474/148‑1 (MQ=255)
                                         aaaaaaTTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTc                                                                                                       >  1:2798661/1‑148 (MQ=255)
                                         aaaaaaTTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTc                                                                                                       >  1:2798671/1‑148 (MQ=255)
                                         aaaaaaTTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTc                                                                                                       <  2:413064/148‑1 (MQ=255)
                                             aaTTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATAc                                                                                                   >  2:3520783/1‑148 (MQ=255)
                                             aaTTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATAc                                                                                                   >  2:591430/1‑148 (MQ=255)
                                               ttAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTc                                                                                                 >  2:946500/1‑148 (MQ=255)
                                                tAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTcc                                                                                                >  1:3263964/1‑148 (MQ=255)
                                                tAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTcc                                                                                                >  2:2106472/1‑148 (MQ=255)
                                                tAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTcc                                                                                                <  2:3029784/148‑1 (MQ=255)
                                                tAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTcc                                                                                                >  1:1492745/1‑148 (MQ=255)
                                                 aaaGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTccc                                                                                               <  2:2466923/148‑1 (MQ=255)
                                                 aaaGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTATGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTccc                                                                                               >  1:436168/1‑148 (MQ=255)
                                                  aatattaattaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTcccc                                                                                              <  2:2058524/145‑1 (MQ=255)
                                                  aaGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTcccc                                                                                              <  1:1053968/148‑1 (MQ=255)
                                                  aaGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTcccc                                                                                              >  1:1489885/1‑148 (MQ=255)
                                                   aGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCt                                                                                             >  1:3365347/1‑148 (MQ=255)
                                                     attaattaTCTGTTCATCGGCATTCTGGCTCTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGa                                                                                           <  2:1981639/148‑1 (MQ=255)
                                                     attaattaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGa                                                                                           >  1:2636861/1‑148 (MQ=255)
                                                       taattaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACt                                                                                         <  2:1862217/148‑1 (MQ=255)
                                                       taattaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACt                                                                                         >  2:3486489/1‑148 (MQ=255)
                                                        aattaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACtt                                                                                        <  1:2429313/148‑1 (MQ=255)
                                                         attaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTta                                                                                       <  2:3084772/148‑1 (MQ=255)
                                                         attaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTta                                                                                       <  1:1424612/148‑1 (MQ=255)
                                                          ttaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTtat                                                                                      >  2:2742622/1‑148 (MQ=255)
                                                            aTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATaa                                                                                    >  2:2333321/1‑148 (MQ=255)
                                                             tCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAAc                                                                                   >  2:3238463/1‑148 (MQ=255)
                                                               tGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGa                                                                                 >  1:1156664/1‑148 (MQ=255)
                                                                gTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGaa                                                                                <  2:181959/148‑1 (MQ=255)
                                                                gTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGaa                                                                                >  1:887462/1‑148 (MQ=255)
                                                                 ttCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGaaa                                                                               >  1:1428631/1‑148 (MQ=255)
                                                                    aTCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCa                                                                            >  1:3157097/1‑148 (MQ=255)
                                                                     tCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTNTAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCaa                                                                           <  1:1810643/148‑1 (MQ=255)
                                                                      cGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAAc                                                                          <  1:310093/148‑1 (MQ=255)
                                                                         cATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACggg                                                                       >  2:2805692/1‑148 (MQ=255)
                                                                           ttCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGaa                                                                     >  1:1930989/1‑148 (MQ=255)
                                                                           ttCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGaa                                                                     <  1:1987903/148‑1 (MQ=255)
                                                                            tCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGaaa                                                                    >  2:2973383/1‑148 (MQ=255)
                                                                              tGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAAcc                                                                  <  1:976460/148‑1 (MQ=255)
                                                                              tGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAAcc                                                                  <  2:1961810/148‑1 (MQ=255)
                                                                               ggCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCt                                                                 <  2:609264/148‑1 (MQ=255)
                                                                                gCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCtt                                                                >  1:1956916/1‑148 (MQ=255)
                                                                                   ctgctgCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTg                                                             >  1:796399/1‑148 (MQ=255)
                                                                                     gctgctCGCGGTCGCTCTCTTGCCATCCAATACCTGGTTTGGTAAATCCGACAACCCTATAGCCGCCCATCAAGCGCGGGGAGAGTTGCGTGTGTGCACCATTCATAATACCCAGACATATAAACAAATAAAACGGAAACCATTTTGc                                                           >  1:3279290/1‑148 (MQ=255)
                                                                                     gctgctCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGc                                                           <  1:1050232/148‑1 (MQ=255)
                                                                                       tgctCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCt                                                         >  2:3027005/1‑148 (MQ=255)
                                                                                         ctCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTgg                                                       <  1:1992708/148‑1 (MQ=255)
                                                                                         ctCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTgg                                                       <  1:1717759/148‑1 (MQ=255)
                                                                                          tCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGa                                                      <  2:608323/148‑1 (MQ=255)
                                                                                                       ctGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCg                                         <  2:3231156/148‑1 (MQ=255)
                                                                                                          gCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGaaa                                      <  1:1217610/148‑1 (MQ=255)
                                                                                                          gCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGaaa                                      >  1:246092/1‑148 (MQ=255)
                                                                                                          gCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGaaa                                      <  1:1214574/148‑1 (MQ=255)
                                                                                                          gCCATCCAGTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGaaa                                      <  1:3297975/148‑1 (MQ=255)
                                                                                                              tccatTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGt                                  >  2:2514675/1‑148 (MQ=255)
                                                                                                              tccatTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGt                                  >  2:2634826/1‑148 (MQ=255)
                                                                                                                      cTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGTGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGAt                          <  2:2107574/148‑1 (MQ=255)
                                                                                                                      cTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTGTTGGCCTGGATTACGAACAGGCGAAACAGTTTGCCGAt                          >  2:3249045/1‑148 (MQ=255)
                                                                                                                       tGGTTTGTTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGAtt                         <  2:1815010/148‑1 (MQ=255)
                                                                                                                       tGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGAtt                         >  1:227772/1‑148 (MQ=255)
                                                                                                                       tGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGAtt                         >  2:584806/1‑148 (MQ=255)
                                                                                                                          tttGGTAAAGCCGTCAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTAcc                      <  2:257515/148‑1 (MQ=255)
                                                                                                                           ttGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCt                     <  1:126573/148‑1 (MQ=255)
                                                                                                                           ttGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCt                     <  1:1782059/148‑1 (MQ=255)
                                                                                                                           ttGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCt                     <  1:2779903/148‑1 (MQ=255)
                                                                                                                              gTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCgg                  <  1:3391447/148‑1 (MQ=255)
                                                                                                                                aaaGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCg                <  2:1001753/148‑1 (MQ=255)
                                                                                                                                     cGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTaaaa           <  2:2451651/148‑1 (MQ=255)
                                                                                                                                      gACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAAc          <  1:97360/148‑1 (MQ=255)
                                                                                                                                          aCCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGaa      >  1:2984824/1‑148 (MQ=255)
                                                                                                                                           ccGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGaaa     >  2:749693/1‑148 (MQ=255)
                                                                                                                                              tataGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGTg  <  2:3157097/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGTG  >  CP009273/2689122‑2689411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑