Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,692,793 C→A 100% H226N (CAT→AAT)  yfhH → putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,692,7930CA96.1% 528.8 / 12.3 179H226N (CAT→AAT) yfhHputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (4/3);  new base A (85/87);  total (89/90)
Fisher's exact test for biased strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 179 total aligned reads displayed.

CGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTATTCGTCAT  >  CP009273/2692647‑2692936
                                                                                                                                                  |                                                                                                                                               
cgtcCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTCa                                                                                                                                                <  2:1927809/148‑1 (MQ=255)
 gtcCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAAt                                                                                                                                               <  1:2486031/148‑1 (MQ=255)
   ccccTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTg                                                                                                                                             >  1:287107/1‑148 (MQ=255)
   ccccTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTg                                                                                                                                             >  1:1299808/1‑148 (MQ=255)
    cccTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGc                                                                                                                                            >  2:2123250/1‑148 (MQ=255)
       tGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTg                                                                                                                                         >  1:3344398/1‑148 (MQ=255)
        gACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGt                                                                                                                                        <  2:1539/148‑1 (MQ=255)
             tCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATAcc                                                                                                                                   >  2:1573295/1‑148 (MQ=255)
             tCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATAcc                                                                                                                                   >  2:2560569/1‑148 (MQ=255)
              cTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCa                                                                                                                                  >  1:3333473/1‑148 (MQ=255)
               tGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCAt                                                                                                                                 >  1:3534819/1‑148 (MQ=255)
                 ttattaGCCATTTCCTACACCGGTGTACGACTCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTg                                                                                                                               <  2:2634902/148‑1 (MQ=255)
                 ttattaGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTg                                                                                                                               <  2:946216/148‑1 (MQ=255)
                     taGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCga                                                                                                                           >  1:3126302/1‑148 (MQ=255)
                          aTTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAAc                                                                                                                      <  1:3394463/148‑1 (MQ=255)
                          aTTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAAc                                                                                                                      <  1:2553702/148‑1 (MQ=255)
                            ttCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACaa                                                                                                                    >  2:2667284/1‑148 (MQ=255)
                               cTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCg                                                                                                                 <  1:959495/148‑1 (MQ=255)
                                 acacCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGAc                                                                                                               >  2:3150758/1‑148 (MQ=255)
                                  cacCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACa                                                                                                              >  1:3271410/1‑148 (MQ=255)
                                   ccgggTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGTGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGAAACAGCGTGCTTCTAATTGCCTTTATACCTTTGCCTAATAACAAGCGACaa                                                                                                             <  1:1507813/145‑1 (MQ=255)
                                    ccGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACaaa                                                                                                            <  2:2593002/148‑1 (MQ=255)
                                     cGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAAc                                                                                                           >  1:1999870/1‑148 (MQ=255)
                                      ggTGTACGACGCGAGTTAAACCTGGCGGCAGAGGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACa                                                                                                          >  1:1267323/1‑148 (MQ=255)
                                        tgtACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTATTTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGt                                                                                                        <  2:1558847/148‑1 (MQ=255)
                                        tgtACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGt                                                                                                        <  1:3091405/148‑1 (MQ=255)
                                         gtACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTg                                                                                                       >  1:1632756/1‑148 (MQ=255)
                                          tACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGc                                                                                                      <  1:3321533/148‑1 (MQ=255)
                                          tACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGc                                                                                                      <  2:446597/148‑1 (MQ=255)
                                           acgacgCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCt                                                                                                     >  2:163765/1‑148 (MQ=255)
                                              acgCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCa                                                                                                  <  1:1042950/148‑1 (MQ=255)
                                                   aGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAAtctc                                                                                             >  1:824344/1‑148 (MQ=255)
                                                    gTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATctct                                                                                            >  2:3383736/1‑148 (MQ=255)
                                                    gTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATctct                                                                                            >  2:215760/1‑148 (MQ=255)
                                                     ttAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTg                                                                                           >  1:245771/1‑148 (MQ=255)
                                                     ttAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTg                                                                                           <  2:533993/148‑1 (MQ=255)
                                                      tAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGc                                                                                          <  2:3142701/148‑1 (MQ=255)
                                                      tAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGc                                                                                          >  2:3295305/1‑148 (MQ=255)
                                                        aaCCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCtt                                                                                        <  1:2196027/148‑1 (MQ=255)
                                                         aCCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTg                                                                                       >  1:2516636/1‑148 (MQ=255)
                                                           cTGGCGGCAGATGCTATGCTGCTAGTGGGCGTAAAAGTGCTGGCGATTACCGGCTTTACTCCGATTGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTc                                                                                     <  1:3089267/148‑1 (MQ=255)
                                                            tGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCa                                                                                    <  1:3555696/148‑1 (MQ=255)
                                                            tGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCa                                                                                    <  2:553454/148‑1 (MQ=255)
                                                                ggcAGTTGAGATGCTGCGAGTGGGCGGAATAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCt                                                                                <  1:2341675/148‑1 (MQ=255)
                                                                ggcAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCt                                                                                <  1:2341691/148‑1 (MQ=255)
                                                                ggcAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCt                                                                                <  1:3226147/148‑1 (MQ=255)
                                                                  cAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCa                                                                              <  2:1252551/148‑1 (MQ=255)
                                                                     atgagatgCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAggg                                                                           >  2:295855/1‑148 (MQ=255)
                                                                          atgCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGt                                                                      <  2:1971153/148‑1 (MQ=255)
                                                                           tgctgcGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGACTGtt                                                                     >  1:983635/1‑148 (MQ=255)
                                                                            gctgcGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTa                                                                    >  2:1546045/1‑148 (MQ=255)
                                                                            gctgcGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTa                                                                    <  2:137949/148‑1 (MQ=255)
                                                                            gctgcGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTa                                                                    <  1:1469932/148‑1 (MQ=255)
                                                                               gcGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACg                                                                 >  1:3172961/1‑148 (MQ=255)
                                                                                cGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACgg                                                                <  2:104711/148‑1 (MQ=255)
                                                                                       gCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCt                                                         <  2:3408458/148‑1 (MQ=255)
                                                                                         ggAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGt                                                       <  2:727272/148‑1 (MQ=255)
                                                                                         ggAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGt                                                       <  2:727445/148‑1 (MQ=255)
                                                                                             aaGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCAt                                                   >  1:1002418/1‑148 (MQ=255)
                                                                                               gTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTg                                                 >  1:71706/1‑148 (MQ=255)
                                                                                                    ggCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGACTGTTAACGGATTTGCTGTTCATTGCGCTg                                            >  1:280396/1‑148 (MQ=255)
                                                                                                    ggCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTg                                            <  1:1633478/148‑1 (MQ=255)
                                                                                                    ggCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTg                                            <  1:364517/148‑1 (MQ=255)
                                                                                                     gCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGACTGTTAACGGATTTGCTGTTCATTGCGCTGa                                           >  1:2372825/1‑148 (MQ=255)
                                                                                                        aTTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTc                                        >  2:516686/1‑148 (MQ=255)
                                                                                                           aCCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTcagc                                     <  1:400926/148‑1 (MQ=255)
                                                                                                           aCCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTcagc                                     <  2:3333473/148‑1 (MQ=255)
                                                                                                            ccGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTcagca                                    <  1:117327/148‑1 (MQ=255)
                                                                                                              ggCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAGCAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGACTGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAgg                                  >  1:521257/1‑148 (MQ=255)
                                                                                                               gCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGa                                 >  1:3510322/1‑148 (MQ=255)
                                                                                                               gCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGa                                 >  2:1877425/1‑148 (MQ=255)
                                                                                                               gCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGa                                 >  2:74139/1‑148 (MQ=255)
                                                                                                                cTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGAt                                <  2:3267321/148‑1 (MQ=255)
                                                                                                                  ttACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCt                              <  2:1856239/148‑1 (MQ=255)
                                                                                                                  ttACTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCt                              <  2:3031802/148‑1 (MQ=255)
                                                                                                                    aCTCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTgg                            <  1:3165289/148‑1 (MQ=255)
                                                                                                                      tCCGAATGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGaa                          <  1:2057899/148‑1 (MQ=255)
                                                                                                                          aaTGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTgg                      >  2:2805500/1‑148 (MQ=255)
                                                                                                                          aaTGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTgg                      >  2:3195836/1‑148 (MQ=255)
                                                                                                                            tGCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGACCTGGCa                    >  1:691409/1‑148 (MQ=255)
                                                                                                                             gCCCTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGACTGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCAc                   >  1:798925/1‑148 (MQ=255)
                                                                                                                              cccTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCAcc                  <  2:1262656/148‑1 (MQ=255)
                                                                                                                              cccTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGACCTGGAACTGGCAcc                  >  2:2492031/1‑148 (MQ=255)
                                                                                                                                cTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGACTGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAg                >  1:585456/1‑148 (MQ=255)
                                                                                                                                cTGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAg                >  2:1181497/1‑148 (MQ=255)
                                                                                                                                 tGCAACAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGACTGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGa               >  2:874808/1‑148 (MQ=255)
                                                                                                                                     aCAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGt           >  2:1327334/1‑148 (MQ=255)
                                                                                                                                     aCAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGt           >  2:413221/1‑148 (MQ=255)
                                                                                                                                      cAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACATTGCTTCAATCTCTGCTTGTAAAGCTAAGTGAATGTTAACGGATTTTCTGTTCATTGATCTGATTCAGCAGGATCTCGAACTGGCACCAGAACGTa          >  2:3519947/1‑148 (MQ=255)
                                                                                                                                      cAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTa          >  1:2262125/1‑148 (MQ=255)
                                                                                                                                      cAGCGTGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTa          <  2:1147159/148‑1 (MQ=255)
                                                                                                                                        gCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTAtt        <  1:2268182/148‑1 (MQ=255)
                                                                                                                                           tGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTATTCGt     <  2:3322493/148‑1 (MQ=255)
                                                                                                                                           tGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTATTCGt     >  1:1905333/1‑148 (MQ=255)
                                                                                                                                           tGCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTATTCGt     >  1:1905321/1‑148 (MQ=255)
                                                                                                                                            gCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTATTCGTc    >  1:2853486/1‑148 (MQ=255)
                                                                                                                                            gCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTATTCGTc    >  1:2869569/1‑148 (MQ=255)
                                                                                                                                            gCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTATTCGTc    >  1:1090270/1‑148 (MQ=255)
                                                                                                                                            gCTTCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTATTCGTc    >  1:1833022/1‑148 (MQ=255)
                                                                                                                                              ttCTAATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTATTCGTCAt  <  1:1352765/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTCATTGCCTGTATACCATTGCCGAAGAACAAGCGACAAACAGTGCTTCAATCTCTGCTTGTCACGCTCAGGGAATGTTAACGGATTTGCTGTTCATTGCGCTGATTCAGCAGGATCTGGAACTGGCACCAGAACGTATTCGTCAT  >  CP009273/2692647‑2692936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑