Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,699,380 C→A 100% P368P (CCG→CCT)  lepA ← back‑translocating elongation factor EF4, GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,699,3800CA96.9% 684.5 / 11.3 223P368P (CCG→CCT) lepAback‑translocating elongation factor EF4, GTPase
Reads supporting (aligned to +/- strand):  ref base C (2/5);  new base A (92/124);  total (94/129)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 223 total aligned reads displayed.

TGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCG  >  CP009273/2699235‑2699524
                                                                                                                                                 |                                                                                                                                                
        tatGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAgtgg                                                                                                                                        <  2:1415967/148‑1 (MQ=255)
        tatGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAgtgg                                                                                                                                        <  2:3521773/148‑1 (MQ=255)
         atGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAgtggt                                                                                                                                       >  1:1788301/1‑148 (MQ=255)
           gCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGa                                                                                                                                     <  2:1529162/148‑1 (MQ=255)
            ccTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGAt                                                                                                                                    <  2:1475979/148‑1 (MQ=255)
            ccTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGAt                                                                                                                                    <  2:653176/148‑1 (MQ=255)
            ccTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGAt                                                                                                                                    <  2:3246457/148‑1 (MQ=255)
             cTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATc                                                                                                                                   <  2:2416954/148‑1 (MQ=255)
               gCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATcag                                                                                                                                 <  1:2661756/148‑1 (MQ=255)
                  gcagcagcaTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATcagatc                                                                                                                              >  2:2695815/1‑148 (MQ=255)
                   cagcagcaTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATcagatcc                                                                                                                             <  1:1099181/148‑1 (MQ=255)
                     gcagcaTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATcagatccag                                                                                                                           >  1:2893663/1‑148 (MQ=255)
                      cagcaTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATcagatccaga                                                                                                                          <  1:3027244/148‑1 (MQ=255)
                      cagcaTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATcagatccaga                                                                                                                          <  2:2440231/148‑1 (MQ=255)
                      cagcaTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATcagatccaga                                                                                                                          <  2:20990/148‑1 (MQ=255)
                         caTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCg                                                                                                                       <  2:1665334/148‑1 (MQ=255)
                         caTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCg                                                                                                                       >  2:1894941/1‑148 (MQ=255)
                         caTGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCg                                                                                                                       >  1:2761987/1‑148 (MQ=255)
                           tgtgACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTa                                                                                                                     <  2:2314011/148‑1 (MQ=255)
                               acacTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCa                                                                                                                 <  1:2242218/148‑1 (MQ=255)
                                  ctctGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGt                                                                                                              >  1:664817/1‑148 (MQ=255)
                                      gCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCa                                                                                                          >  1:1726298/1‑148 (MQ=255)
                                      gCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCa                                                                                                          >  1:112227/1‑148 (MQ=255)
                                      gCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCa                                                                                                          >  1:2344264/1‑148 (MQ=255)
                                       cAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAg                                                                                                         <  2:2761987/148‑1 (MQ=255)
                                        aaTCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGa                                                                                                        <  1:776910/148‑1 (MQ=255)
                                        aaTCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGa                                                                                                        <  1:2728292/148‑1 (MQ=255)
                                         aTCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGAc                                                                                                       >  1:3262906/1‑148 (MQ=255)
                                           cGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGTTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGt                                                                                                     >  1:3162831/1‑148 (MQ=255)
                                           cGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGt                                                                                                     >  1:491223/1‑148 (MQ=255)
                                              cTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTcc                                                                                                  >  1:1366252/1‑148 (MQ=255)
                                              cTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTcc                                                                                                  <  1:775304/148‑1 (MQ=255)
                                                  cgcAGTTCGTAGATGTTATTGACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGga                                                                                              <  1:3406380/148‑1 (MQ=255)
                                                      gTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGgatgat                                                                                          <  1:471471/148‑1 (MQ=255)
                                                      gTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGgatgat                                                                                          >  2:268999/1‑148 (MQ=255)
                                                       ttCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGAtc                                                                                         <  1:471280/148‑1 (MQ=255)
                                                       ttCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGAtc                                                                                         <  1:450606/148‑1 (MQ=255)
                                                        tCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGAtct                                                                                        <  1:1139029/148‑1 (MQ=255)
                                                         cGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGAtctc                                                                                       >  2:3386214/1‑148 (MQ=255)
                                                         cGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGAtctc                                                                                       <  2:171418/148‑1 (MQ=255)
                                                         cGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGAtctc                                                                                       >  2:3386763/1‑148 (MQ=255)
                                                           tAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCa                                                                                     <  1:286222/148‑1 (MQ=255)
                                                            aGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGtt                                                                                                    >  2:1685781/1‑132 (MQ=255)
                                                            aGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGtt                                                                                                    <  1:1685781/132‑1 (MQ=255)
                                                            aGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCAt                                                                                    >  1:2551748/1‑148 (MQ=255)
                                                               tGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCAtgtg                                                                                 >  1:2097500/1‑148 (MQ=255)
                                                               tGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCAtgtg                                                                                 >  2:3069703/1‑148 (MQ=255)
                                                                gTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTgc                                                                                <  2:2315926/148‑1 (MQ=255)
                                                                gTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTgc                                                                                <  2:1176542/148‑1 (MQ=255)
                                                                gTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTgc                                                                                <  1:1291009/148‑1 (MQ=255)
                                                                gTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTACATGTgc                                                                                >  1:2619841/1‑148 (MQ=255)
                                                                   aTTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTgcagc                                                                             >  1:2617977/1‑148 (MQ=255)
                                                                    tttACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTgcagca                                                                            >  2:3029648/1‑148 (MQ=255)
                                                                    tttACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTgcagca                                                                            <  2:3359767/148‑1 (MQ=255)
                                                                    tttACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTgcagca                                                                            <  2:644031/148‑1 (MQ=255)
                                                                     ttACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGcagcag                                                                           >  1:2397929/1‑148 (MQ=255)
                                                                       aCCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGc                                                                         <  1:1955972/148‑1 (MQ=255)
                                                                       aCCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGc                                                                         <  1:154152/148‑1 (MQ=255)
                                                                        ccGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGcc                                                                        >  1:1016761/1‑148 (MQ=255)
                                                                         cGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCg                                                                       <  2:3284024/148‑1 (MQ=255)
                                                                          gcaggcagCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGa                                                                      >  2:1011919/1‑148 (MQ=255)
                                                                              gcagCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGaa                                                                  >  1:2504384/1‑148 (MQ=255)
                                                                                agCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGc                                                                <  1:3381973/148‑1 (MQ=255)
                                                                                       aTGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCg                                                         <  2:522228/148‑1 (MQ=255)
                                                                                       aTGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCg                                                         <  1:410262/148‑1 (MQ=255)
                                                                                       aTGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCg                                                         >  2:768895/1‑148 (MQ=255)
                                                                                         gggCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGa                                                       >  2:1840755/1‑148 (MQ=255)
                                                                                         gggCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGa                                                       >  2:2499268/1‑148 (MQ=255)
                                                                                           gCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGaaa                                                     >  1:3522902/1‑148 (MQ=255)
                                                                                           gCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGaaa                                                     <  1:2083314/148‑1 (MQ=255)
                                                                                              gTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCa                                                  <  2:2893663/148‑1 (MQ=255)
                                                                                                cGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCaaa                                                >  2:831535/1‑148 (MQ=255)
                                                                                                  aCGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGc                                              <  2:2504759/148‑1 (MQ=255)
                                                                                                  aCGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGc                                              <  2:1285146/148‑1 (MQ=255)
                                                                                                  aCGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGc                                              >  2:3393711/1‑148 (MQ=255)
                                                                                                   cGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTACTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGcc                                             >  2:326241/1‑148 (MQ=255)
                                                                                                        aTAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAgcg                                        >  2:1753143/1‑148 (MQ=255)
                                                                                                        aTAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAgcg                                        >  2:1513478/1‑148 (MQ=255)
                                                                                                        aTAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAgcg                                        >  2:3075635/1‑148 (MQ=255)
                                                                                                                tctTGACGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAg                                <  2:2830518/148‑1 (MQ=255)
                                                                                                                    gACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCttt                            <  1:1565899/148‑1 (MQ=255)
                                                                                                                     aCGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTc                           <  1:1400401/148‑1 (MQ=255)
                                                                                                                     aCGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTc                           <  1:3546412/148‑1 (MQ=255)
                                                                                                                     aCGTGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTc                           <  2:2617977/148‑1 (MQ=255)
                                                                                                                        tGGTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCgg                        <  2:2282405/148‑1 (MQ=255)
                                                                                                                         ggTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGc                       >  2:338031/1‑148 (MQ=255)
                                                                                                                         ggTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGc                       >  2:338087/1‑148 (MQ=255)
                                                                                                                          gTTTCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCt                      <  1:1657745/148‑1 (MQ=255)
                                                                                                                           tttCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTc                     <  2:835247/148‑1 (MQ=255)
                                                                                                                            ttCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCa                    <  1:1894941/148‑1 (MQ=255)
                                                                                                                            ttCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCa                    <  1:1699101/148‑1 (MQ=255)
                                                                                                                            ttCAACTTCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCa                    >  2:3228199/1‑148 (MQ=255)
                                                                                                                                  ttCATACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAAc              <  1:913442/148‑1 (MQ=255)
                                                                                                                                     aTACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGt           >  2:437160/1‑148 (MQ=255)
                                                                                                                                      tACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTg          <  2:506159/148‑1 (MQ=255)
                                                                                                                                      tACACTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTg          <  2:3158030/148‑1 (MQ=255)
                                                                                                                                         acTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAgg       <  2:94107/148‑1 (MQ=255)
                                                                                                                                          cTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGc      >  1:2595823/1‑148 (MQ=255)
                                                                                                                                          cTACGGTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGc      >  1:800969/1‑148 (MQ=255)
                                                                                                                                              ggTAGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCg  <  1:3386763/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCG  >  CP009273/2699235‑2699524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑