Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,798,195 A→T 100% K8* (AAA→TAA)  proV → glycine betaine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,798,1950AT98.7% 781.7 / ‑0.6 238K8* (AAA→TAA) proVglycine betaine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base T (121/114);  total (123/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 244 total aligned reads displayed.

ATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGA  >  CP009273/2798051‑2798339
                                                                                                                                                |                                                                                                                                                
atatTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTtaaa                                                                                                                                               >  2:640402/1‑148 (MQ=255)
 tatTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTtaaaa                                                                                                                                              >  1:3138421/1‑148 (MQ=255)
 tatTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTtaaaa                                                                                                                                              >  1:2508506/1‑148 (MQ=255)
  atTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAAt                                                                                                                                             <  1:87378/148‑1 (MQ=255)
  atTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAAt                                                                                                                                             <  1:2991294/148‑1 (MQ=255)
   ttCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTATAAATTTAAAATc                                                                                                                                            >  2:2140899/1‑148 (MQ=255)
   ttCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATc                                                                                                                                            >  2:481632/1‑148 (MQ=255)
    tCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCt                                                                                                                                           <  1:2246694/148‑1 (MQ=255)
       tGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTta                                                                                                                                        <  2:295664/148‑1 (MQ=255)
          cacaTTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATaa                                                                                                                                     <  2:789123/148‑1 (MQ=255)
           acaTTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATaaa                                                                                                                                    <  1:296771/148‑1 (MQ=255)
            caTTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATaaaa                                                                                                                                   <  1:2794829/148‑1 (MQ=255)
              tttGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAata                                                                                                                                 <  2:504997/148‑1 (MQ=255)
                  ccATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTg                                                                                                                             <  2:36260/148‑1 (MQ=255)
                  ccATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTg                                                                                                                             <  2:1929774/148‑1 (MQ=255)
                     tCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCg                                                                                                                          >  2:1577941/1‑148 (MQ=255)
                        ggggTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGc                                                                                                                       >  1:1905231/1‑148 (MQ=255)
                          ggTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCAt                                                                                                                     <  1:2714034/148‑1 (MQ=255)
                           gTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATc                                                                                                                    >  1:959103/1‑148 (MQ=255)
                            ttGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATcc                                                                                                                   <  1:568437/148‑1 (MQ=255)
                             tGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCca                                                                                                                  <  2:1100846/148‑1 (MQ=255)
                              gCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCcac                                                                                                                 <  1:142739/148‑1 (MQ=255)
                                cTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACag                                                                                                               <  1:2808039/148‑1 (MQ=255)
                                cTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACag                                                                                                               <  1:2638223/148‑1 (MQ=255)
                                      ttCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACagcgagcg                                                                                                         >  1:2540088/1‑148 (MQ=255)
                                       tctcAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGt                                                                                                        >  2:909621/1‑148 (MQ=255)
                                       tctcAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGt                                                                                                        <  1:1206178/148‑1 (MQ=255)
                                        ctcAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGtt                                                                                                       <  2:1416958/148‑1 (MQ=255)
                                             tATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAat                                                                                                  <  1:2612473/148‑1 (MQ=255)
                                             tATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAat                                                                                                  <  1:2612073/148‑1 (MQ=255)
                                             tATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAat                                                                                                  <  2:3239086/148‑1 (MQ=255)
                                              aTGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAata                                                                                                 >  1:2638043/1‑148 (MQ=255)
                                                 ttAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAatatat                                                                                              <  1:3066810/148‑1 (MQ=255)
                                                  tAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATc                                                                                             >  2:3202748/1‑148 (MQ=255)
                                                     ggTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGaa                                                                                          <  2:2198930/148‑1 (MQ=255)
                                                     ggTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGaa                                                                                          >  1:3314126/1‑148 (MQ=255)
                                                        aGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACaa                                                                                       <  2:1254341/148‑1 (MQ=255)
                                                        aGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACaa                                                                                       <  1:2453152/148‑1 (MQ=255)
                                                            aaaaGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGAc                                                                                   >  1:672526/1‑148 (MQ=255)
                                                               aGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACttt                                                                                >  1:3409291/1‑148 (MQ=255)
                                                                  gACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCaa                                                                             <  1:1258336/148‑1 (MQ=255)
                                                                  gACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCaa                                                                             <  1:3180227/148‑1 (MQ=255)
                                                                       tttCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGaa                                                                        >  2:2440925/1‑148 (MQ=255)
                                                                        ttCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAAc                                                                       >  1:1036182/1‑148 (MQ=255)
                                                                        ttCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAAc                                                                       >  1:1034789/1‑148 (MQ=255)
                                                                        ttCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAAc                                                                       >  1:3094815/1‑148 (MQ=255)
                                                                         tCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACa                                                                      <  2:3314102/148‑1 (MQ=255)
                                                                         tCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACa                                                                      >  1:2875009/1‑148 (MQ=255)
                                                                               gggTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCt                                                                <  1:1337658/148‑1 (MQ=255)
                                                                                  tAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGa                                                             >  1:1765723/1‑148 (MQ=255)
                                                                                      atatCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGaaaaa                                                         <  2:548514/148‑1 (MQ=255)
                                                                                      atatAGACAGAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGaaaaa                                                         <  2:2759002/148‑1 (MQ=255)
                                                                                       tatCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGaaaaaa                                                        >  1:2362071/1‑148 (MQ=255)
                                                                                        atCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAAc                                                       >  1:509714/1‑148 (MQ=255)
                                                                                        atCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAAc                                                       >  1:1683258/1‑148 (MQ=255)
                                                                                         tCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACt                                                      >  2:2681084/1‑148 (MQ=255)
                                                                                            aCATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTggg                                                   <  2:3053725/148‑1 (MQ=255)
                                                                                            aCATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTggg                                                   <  2:3053694/148‑1 (MQ=255)
                                                                                             cATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGc                                                  >  1:178257/1‑148 (MQ=255)
                                                                                                aGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTAt                                               >  2:2716814/1‑148 (MQ=255)
                                                                                                   cAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGc                                            <  2:971419/148‑1 (MQ=255)
                                                                                                    aaaTAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCt                                           <  2:171688/148‑1 (MQ=255)
                                                                                                    aaaTAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCt                                           <  2:1974864/148‑1 (MQ=255)
                                                                                                    aaaTAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCt                                           <  2:2420597/148‑1 (MQ=255)
                                                                                                     aaTAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCtt                                          >  1:3307930/1‑148 (MQ=255)
                                                                                                     aaTAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCtt                                          >  1:2550462/1‑148 (MQ=255)
                                                                                                        aaaGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGc                                       >  2:83767/1‑148 (MQ=255)
                                                                                                         aaGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCg                                      >  2:3449792/1‑148 (MQ=255)
                                                                                                         aaGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCg                                      >  2:1618661/1‑148 (MQ=255)
                                                                                                                cTTTCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGa                               >  2:1587732/1‑148 (MQ=255)
                                                                                                                 tttCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGAc                              >  2:3465221/1‑148 (MQ=255)
                                                                                                                  ttCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACg                             <  1:2838569/148‑1 (MQ=255)
                                                                                                                   tCTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGc                            >  2:2207268/1‑148 (MQ=255)
                                                                                                                    cTATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGcc                           >  1:137096/1‑148 (MQ=255)
                                                                                                                     tATTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCa                          >  1:364554/1‑148 (MQ=255)
                                                                                                                      aTTGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAg                         >  1:1848006/1‑148 (MQ=255)
                                                                                                                       ttGCTTGGAAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGt                        <  1:694038/148‑1 (MQ=255)
                                                                                                                       ttGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGt                        >  1:402590/1‑148 (MQ=255)
                                                                                                                        tGCATGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTc                       >  2:3329295/1‑148 (MQ=255)
                                                                                                                            tGGCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGATATCGCTTGGCGTAAAAGACGCCAGTCTGGc                   >  2:2832609/1‑148 (MQ=255)
                                                                                                                             ggCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAGCTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGcc                  <  1:2096170/148‑1 (MQ=255)
                                                                                                                             ggCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGcc                  >  1:3451959/1‑148 (MQ=255)
                                                                                                                             ggCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGcc                  >  1:2461755/1‑148 (MQ=255)
                                                                                                                              gCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACCGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCa                 <  2:2991349/148‑1 (MQ=255)
                                                                                                                              gCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCa                 <  2:2936026/148‑1 (MQ=255)
                                                                                                                              gCAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCa                 >  2:1861318/1‑148 (MQ=255)
                                                                                                                               cAATTAAATTAGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCAt                <  1:1738413/148‑1 (MQ=255)
                                                                                                                                 attaaattaGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTg              >  1:950057/1‑148 (MQ=255)
                                                                                                                                 attaaattaGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTg              >  1:1399834/1‑148 (MQ=255)
                                                                                                                                   taaattaGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTgaa            >  1:1504309/1‑148 (MQ=255)
                                                                                                                                   taaattaGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTgaa            >  2:1867224/1‑148 (MQ=255)
                                                                                                                                     aattaGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTgaaga          <  2:3000698/148‑1 (MQ=255)
                                                                                                                                     aattaGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTACAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGTGTAAAAGACGCCAGTCTGGCCATTgaaga          <  2:404674/148‑1 (MQ=255)
                                                                                                                                      attaGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTgaagaa         <  1:543152/148‑1 (MQ=255)
                                                                                                                                       ttaGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGaagaag        >  2:1496967/1‑148 (MQ=255)
                                                                                                                                        taGAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAgg       >  2:3153874/1‑148 (MQ=255)
                                                                                                                                          gAAATTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCg     <  1:1138249/148‑1 (MQ=255)
                                                                                                                                           aaaTTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCga    >  2:136674/1‑148 (MQ=255)
                                                                                                                                           aaaTTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCga    <  2:672526/148‑1 (MQ=255)
                                                                                                                                             aTTTAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCgaga  <  2:2545398/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
ATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGA  >  CP009273/2798051‑2798339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑