Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,828,952 C→A 100% T611T (ACG→ACT)  hypF ← carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,828,9520CA97.7% 527.7 / 1.4 193T611T (ACG→ACT) hypFcarbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases
Reads supporting (aligned to +/- strand):  ref base C (2/5);  new base A (98/88);  total (100/93)
Fisher's exact test for biased strand distribution p-value = 2.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 193 total aligned reads displayed.

TGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAA  >  CP009273/2828808‑2829096
                                                                                                                                                |                                                                                                                                                
tgcctgccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtg                                                                                                                                               <  1:404853/148‑1 (MQ=255)
  cctgccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtggc                                                                                                                                             <  2:2319815/148‑1 (MQ=255)
  cctgccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtggc                                                                                                                                             >  1:130229/1‑148 (MQ=255)
    tgccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtggctg                                                                                                                                           <  1:2273466/148‑1 (MQ=255)
    tgccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtggct                                                                                                                                            >  2:858078/1‑147 (MQ=255)
    tgccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCAACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGCCATGTGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtggctg                                                                                                                                           >  1:2408733/1‑148 (MQ=255)
     gccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtggctgg                                                                                                                                          <  2:1392641/148‑1 (MQ=255)
     gccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtggctgg                                                                                                                                          <  2:1094731/148‑1 (MQ=255)
     gccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtggctgg                                                                                                                                          <  2:1094282/148‑1 (MQ=255)
     gccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtggctgg                                                                                                                                          >  2:3140434/1‑148 (MQ=255)
        aGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGgcgc                                                                                                                                       >  2:746889/1‑148 (MQ=255)
          ttCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGcac                                                                                                                                     <  1:2875648/148‑1 (MQ=255)
          ttCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGcac                                                                                                                                     >  2:731194/1‑148 (MQ=255)
            cAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAg                                                                                                                                   >  2:933090/1‑148 (MQ=255)
             aGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGc                                                                                                                                  <  2:1953070/148‑1 (MQ=255)
              gCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGcc                                                                                                                                 >  1:807865/1‑148 (MQ=255)
              gCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAAcc                                                                                                                                 <  2:1504570/148‑1 (MQ=255)
                cACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCa                                                                                                                               <  1:841210/148‑1 (MQ=255)
                 aCTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAg                                                                                                                              <  1:1830968/148‑1 (MQ=255)
                   tgctgcCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGGATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTg                                                                                                                            <  1:1653939/148‑1 (MQ=255)
                   tgctgcCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTg                                                                                                                            <  1:1654232/148‑1 (MQ=255)
                   tgctgcCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTg                                                                                                                            >  2:891724/1‑148 (MQ=255)
                    gctgcCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTgc                                                                                                                           >  2:67982/1‑148 (MQ=255)
                             aaGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCAc                                                                                                                  >  1:1422777/1‑148 (MQ=255)
                              aGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACt                                                                                                                 >  1:2673653/1‑148 (MQ=255)
                              aGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACt                                                                                                                 >  1:2322475/1‑148 (MQ=255)
                                tGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGc                                                                                                               <  2:3253906/148‑1 (MQ=255)
                                     agaTCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGa                                                                                                          >  2:1066916/1‑148 (MQ=255)
                                      gaTCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGaa                                                                                                         >  1:430911/1‑148 (MQ=255)
                                         ccAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGaaaaa                                                                                                      >  1:3163247/1‑148 (MQ=255)
                                           aGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAAc                                                                                                    >  2:1866137/1‑148 (MQ=255)
                                            gTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACg                                                                                                   >  1:532284/1‑148 (MQ=255)
                                            gTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACg                                                                                                   <  2:3169664/148‑1 (MQ=255)
                                             ttGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGc                                                                                                  <  1:2624718/148‑1 (MQ=255)
                                              tGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGcc                                                                                                 <  2:1116579/148‑1 (MQ=255)
                                                  tGTCCACCCGCGGCATTGACACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCcaca                                                                                             <  1:856695/148‑1 (MQ=255)
                                                     ccACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGa                                                                                          <  1:1503187/148‑1 (MQ=255)
                                                      cACCCGCGGCTTTGTCACCGTATGCGTCACTCGGTGGCTTGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGATTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGAt                                                                                         <  1:913429/148‑1 (MQ=255)
                                                      cACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGAt                                                                                         >  2:1818997/1‑148 (MQ=255)
                                                       aCCCGCGGCATTGTCCCCGGAGGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATg                                                                                        <  1:2795427/148‑1 (MQ=255)
                                                          cgcgGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACg                                                                                     <  1:2025800/148‑1 (MQ=255)
                                                          cgcgGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACg                                                                                     <  1:2025872/148‑1 (MQ=255)
                                                           gcgGCTTTGTCACGGGATGCGTCACTCCGTGGCATGTGGCTGCGAGCGCCTCCAGAGCACAAGCCGATTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGc                                                                                    <  1:2724870/148‑1 (MQ=255)
                                                           gcgGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGc                                                                                    <  2:1801429/148‑1 (MQ=255)
                                                              gCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAg                                                                                 >  2:3236705/1‑148 (MQ=255)
                                                               cATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAgc                                                                                >  2:2386342/1‑148 (MQ=255)
                                                                 ttGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAgcgg                                                                              >  2:3520534/1‑148 (MQ=255)
                                                                  tGTCACCGGATGCGTCACTCCGGGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAgcggc                                                                             <  2:3318970/148‑1 (MQ=255)
                                                                     cACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGcgcg                                                                          <  2:2330883/148‑1 (MQ=255)
                                                                      aCCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGt                                                                         <  2:2843266/148‑1 (MQ=255)
                                                                      aCCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGt                                                                         >  2:1074261/1‑148 (MQ=255)
                                                                           aTGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAAtt                                                                    >  1:735928/1‑148 (MQ=255)
                                                                             gCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTcc                                                                  >  2:2124303/1‑148 (MQ=255)
                                                                             gCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTACAGAGCACAAGCCGCTTCACATACATAACTTAAAGTGGCTGTCGCACAGCCCAGTGCCGCAGCCAATGCATCGAAAAAACCACCACACGATGACTCCAGCGGCGCGTCAAtaac                                                                  >  2:2930227/1‑145 (MQ=255)
                                                                               gTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCAc                                                                <  1:581061/148‑1 (MQ=255)
                                                                                tCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACg                                                               >  2:1891849/1‑148 (MQ=255)
                                                                                     ccGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCaa                                                          <  2:1591850/148‑1 (MQ=255)
                                                                                     ccGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCaa                                                          >  2:2608851/1‑148 (MQ=255)
                                                                                        tGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATgg                                                       >  1:447138/1‑148 (MQ=255)
                                                                                          gCATGAGGCTGCGAGCGCCTCCAGCGCAAAAGCGGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCTAAAACACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGcc                                                     <  1:66108/148‑1 (MQ=255)
                                                                                          gCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGcc                                                     <  2:1023698/148‑1 (MQ=255)
                                                                                           cATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGccc                                                    >  2:2167248/1‑148 (MQ=255)
                                                                                             tGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCcgc                                                  >  2:2002418/1‑148 (MQ=255)
                                                                                             tGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCcgc                                                  >  2:2131462/1‑148 (MQ=255)
                                                                                               aGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCgcgc                                                <  1:1114060/148‑1 (MQ=255)
                                                                                               aGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCgcgc                                                <  1:155467/148‑1 (MQ=255)
                                                                                                       aGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGc                                        >  1:3530949/1‑148 (MQ=255)
                                                                                                       aGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGc                                        >  1:3530849/1‑148 (MQ=255)
                                                                                                         cgcCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTc                                      >  1:1533881/1‑148 (MQ=255)
                                                                                                         cgcCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTc                                      >  2:2959815/1‑148 (MQ=255)
                                                                                                         cgcCTCCAGAGCACAAGCCGCTTCACCTGCATAACGGCCAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAACAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTc                                      <  2:3481222/148‑1 (MQ=255)
                                                                                                              ccAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGtt                                 <  1:864483/148‑1 (MQ=255)
                                                                                                               cAGAGCACACGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGAATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGttt                                <  1:1227404/148‑1 (MQ=255)
                                                                                                               cAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGttt                                <  1:2334143/148‑1 (MQ=255)
                                                                                                               cAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGttt                                <  1:3520282/148‑1 (MQ=255)
                                                                                                                  agCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGc                             >  1:1982128/1‑148 (MQ=255)
                                                                                                                    cacaAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTg                           <  1:1979609/148‑1 (MQ=255)
                                                                                                                      caAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGtt                         >  1:247528/1‑148 (MQ=255)
                                                                                                                      caAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATAGCCCGCGCCAGCACGCTCCAGTTTTGCTGtt                         >  1:2438572/1‑148 (MQ=255)
                                                                                                                          cggCTACACCTTCATAACTTATAGGGGGTGGCGCACAGCCCTGGGCCGCGGCCACTGAATCTAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGcac                     <  1:746793/146‑1 (MQ=255)
                                                                                                                          ccGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGcac                     <  1:933090/148‑1 (MQ=255)
                                                                                                                           cGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGcaca                    >  1:3194251/1‑148 (MQ=255)
                                                                                                                             cTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACt                  >  2:90591/1‑148 (MQ=255)
                                                                                                                              ttCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACtt                 >  1:2144845/1‑148 (MQ=255)
                                                                                                                                cACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGc               >  2:3455344/1‑148 (MQ=255)
                                                                                                                                cACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGc               >  2:3455326/1‑148 (MQ=255)
                                                                                                                                  ccTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTg             >  1:1159217/1‑148 (MQ=255)
                                                                                                                                   cTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGt            >  2:3560380/1‑148 (MQ=255)
                                                                                                                                     tCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGttt          >  1:467735/1‑148 (MQ=255)
                                                                                                                                      cATAACTTAAAGGGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTc         <  2:2169839/148‑1 (MQ=255)
                                                                                                                                        tAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCag       >  2:170135/1‑148 (MQ=255)
                                                                                                                                        tAACTTAAAGTGGCTGGCGCACAGCCCAGGGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCag       <  1:1858675/148‑1 (MQ=255)
                                                                                                                                           cTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGt    >  1:2738302/1‑148 (MQ=255)
                                                                                                                                            ttAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTa   <  1:2475028/148‑1 (MQ=255)
                                                                                                                                            ttAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTa   <  2:2169660/148‑1 (MQ=255)
                                                                                                                                             tAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTaa  <  1:3477183/148‑1 (MQ=255)
                                                                                                                                             tAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTaa  <  1:524440/148‑1 (MQ=255)
                                                                                                                                             tAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTaa  <  1:617039/148‑1 (MQ=255)
                                                                                                                                             tAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTaa  <  2:2472673/148‑1 (MQ=255)
                                                                                                                                             tAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGAACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTaa  >  2:1328870/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAA  >  CP009273/2828808‑2829096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑