Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,835,094 C→A 100% P249Q (CCG→CAG)  ascB → cryptic 6‑phospho‑beta‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,835,0940CA98.2% 509.3 / 0.8 163P249Q (CCG→CAG) ascBcryptic 6‑phospho‑beta‑glucosidase
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base A (81/79);  total (81/82)
Fisher's exact test for biased strand distribution p-value = 2.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 163 total aligned reads displayed.

AATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCCGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGAT  >  CP009273/2834949‑2835236
                                                                                                                                                 |                                                                                                                                              
aaTCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAgg                                                                                                                                              <  2:153955/148‑1 (MQ=255)
 atcaggatcaggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGa                                                                                                                                             >  1:1576676/1‑148 (MQ=255)
 atcaggatcaggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGa                                                                                                                                             >  2:2320931/1‑148 (MQ=255)
   caggatcaggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAg                                                                                                                                           <  1:2980892/148‑1 (MQ=255)
    aggatcaggTGAAATATCAGGCCGCGCATCACCAGCTGTTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGATGGCTGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGATGa                                                                                                                                          <  2:1424938/148‑1 (MQ=255)
    aggatcaggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGa                                                                                                                                          <  1:835504/148‑1 (MQ=255)
     ggatcaggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGAt                                                                                                                                         >  2:2630360/1‑148 (MQ=255)
     ggatcaggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGAt                                                                                                                                         >  2:1832716/1‑148 (MQ=255)
        tcaggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTc                                                                                                                                      >  1:867838/1‑148 (MQ=255)
        tcaggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTc                                                                                                                                      >  1:2671123/1‑148 (MQ=255)
        tcaggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTc                                                                                                                                      >  1:2671161/1‑148 (MQ=255)
        tcaggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTc                                                                                                                                      >  1:2929055/1‑148 (MQ=255)
         caggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCt                                                                                                                                     >  2:1534611/1‑148 (MQ=255)
         caggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCt                                                                                                                                     >  2:1534625/1‑148 (MQ=255)
               aaaTATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGggcgg                                                                                                                               >  2:3372577/1‑148 (MQ=255)
                      aGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGga                                                                                                                        >  1:667649/1‑148 (MQ=255)
                      aGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGAAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGga                                                                                                                        >  1:1554199/1‑148 (MQ=255)
                        gCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGgaga                                                                                                                      >  2:2581247/1‑148 (MQ=255)
                           gcgcATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAg                                                                                                                   >  1:2638664/1‑148 (MQ=255)
                             gcATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGAc                                                                                                                 <  2:1188369/148‑1 (MQ=255)
                              cATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCACGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGAcc                                                                                                                >  2:2456640/1‑148 (MQ=255)
                              cATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAACGAcc                                                                                                                >  1:1852310/1‑148 (MQ=255)
                              cATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGAcc                                                                                                                >  2:3484572/1‑148 (MQ=255)
                               aTCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCg                                                                                                               <  2:3518221/148‑1 (MQ=255)
                               aTCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCg                                                                                                               <  1:1880534/148‑1 (MQ=255)
                                  aCCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGa                                                                                                            >  1:480865/1‑148 (MQ=255)
                                   ccAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGaa                                                                                                           >  1:3312008/1‑148 (MQ=255)
                                    cAGCTGGTTGCAAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGaaa                                                                                                          >  1:3479683/1‑148 (MQ=255)
                                     aGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGaaaa                                                                                                         >  2:622878/1‑148 (MQ=255)
                                         ggTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTg                                                                                                     <  1:2389394/148‑1 (MQ=255)
                                         ggTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTg                                                                                                     >  2:81742/1‑148 (MQ=255)
                                               cAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGtttttt                                                                                               <  1:3155948/148‑1 (MQ=255)
                                               cAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGtttttt                                                                                               <  1:3156000/148‑1 (MQ=255)
                                                aGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTa                                                                                              <  2:48291/148‑1 (MQ=255)
                                                 gTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTAt                                                                                             <  1:2323683/148‑1 (MQ=255)
                                                 gTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTAt                                                                                             <  2:3424284/148‑1 (MQ=255)
                                                 gTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTAt                                                                                             <  1:778520/148‑1 (MQ=255)
                                                    cgcTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGa                                                                                          <  1:3175679/148‑1 (MQ=255)
                                                    cgcTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGa                                                                                          <  2:1210311/148‑1 (MQ=255)
                                                    cgcTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGa                                                                                          <  1:971781/148‑1 (MQ=255)
                                                      cTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTAGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGAtg                                                                                        <  1:10563/148‑1 (MQ=255)
                                                        aGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGAtgtg                                                                                      <  2:2663191/148‑1 (MQ=255)
                                                         gCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGACAACCTGTTTTTTATCGATGTGc                                                                                     >  2:1588054/1‑148 (MQ=255)
                                                         gCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGc                                                                                     >  1:2928173/1‑148 (MQ=255)
                                                         gCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGc                                                                                     >  2:1588080/1‑148 (MQ=255)
                                                          ccaccaAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCa                                                                                    >  1:1340550/1‑148 (MQ=255)
                                                               aaaaTCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGgcgc                                                                               >  2:3334312/1‑148 (MQ=255)
                                                                 aaTCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCgg                                                                             <  1:1872608/148‑1 (MQ=255)
                                                                 aaTCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCgg                                                                             >  2:3232872/1‑148 (MQ=255)
                                                                  aTCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCggg                                                                            >  2:3038455/1‑148 (MQ=255)
                                                                    cGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGc                                                                          >  1:2187798/1‑148 (MQ=255)
                                                                     gCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCa                                                                         >  2:113773/1‑148 (MQ=255)
                                                                     gCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCa                                                                         >  2:113806/1‑148 (MQ=255)
                                                                     gCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCa                                                                         <  2:3061123/148‑1 (MQ=255)
                                                                      cccATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCAc                                                                        >  2:1620984/1‑148 (MQ=255)
                                                                      cccATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCAc                                                                        >  2:1620759/1‑148 (MQ=255)
                                                                         aTGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTa                                                                     >  2:2994715/1‑148 (MQ=255)
                                                                            aGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATcc                                                                  >  2:476058/1‑148 (MQ=255)
                                                                            aGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATcc                                                                  <  1:3041108/148‑1 (MQ=255)
                                                                             ggTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCg                                                                 >  1:2280208/1‑148 (MQ=255)
                                                                             ggTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCg                                                                 >  1:2280620/1‑148 (MQ=255)
                                                                               ttAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGc                                                               <  1:502271/148‑1 (MQ=255)
                                                                                 aaCCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCtt                                                             <  2:329932/148‑1 (MQ=255)
                                                                                  aCCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTa                                                            <  1:1740084/148‑1 (MQ=255)
                                                                                    ccGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTAct                                                          >  2:2285493/1‑148 (MQ=255)
                                                                                     cGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTActc                                                         <  1:1851632/148‑1 (MQ=255)
                                                                                     cGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTActc                                                         <  2:2346994/148‑1 (MQ=255)
                                                                                         aaaTCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGcc                                                     <  2:2929055/148‑1 (MQ=255)
                                                                                            tCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCcgc                                                  >  1:3362690/1‑148 (MQ=255)
                                                                                              aGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGt                                                <  1:427199/148‑1 (MQ=255)
                                                                                               ggTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCCGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTa                                               <  1:547106/148‑1 (MQ=255)
                                                                                               ggTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGtc                                               >  2:1890319/1‑147 (MQ=255)
                                                                                                gTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTAt                                              >  2:3476568/1‑148 (MQ=255)
                                                                                                gTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTAt                                              >  2:1149020/1‑148 (MQ=255)
                                                                                                   ggCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTcc                                           >  2:1680935/1‑148 (MQ=255)
                                                                                                    gCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCcg                                          >  1:1651896/1‑148 (MQ=255)
                                                                                                     cTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCcgc                                         >  1:2549486/1‑148 (MQ=255)
                                                                                                          tGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGaaaa                                    <  2:375011/148‑1 (MQ=255)
                                                                                                              ggcgggcgGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAggg                                >  1:2978878/1‑148 (MQ=255)
                                                                                                               gcgggcggTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAgggg                               <  1:218359/148‑1 (MQ=255)
                                                                                                               gcgggcggTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAgggg                               >  2:578590/1‑148 (MQ=255)
                                                                                                               gcgggcggTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAgggg                               >  1:3134441/1‑148 (MQ=255)
                                                                                                               gcgggcggTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAgggg                               <  1:3372577/148‑1 (MQ=255)
                                                                                                                cgggcggTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGt                              <  2:846946/148‑1 (MQ=255)
                                                                                                                   gcggTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAAc                           >  2:1046820/1‑148 (MQ=255)
                                                                                                                           ttCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATcaaca                   <  2:2198594/148‑1 (MQ=255)
                                                                                                                            tCTACCCTTACAGTTTCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATcaacac                  <  2:2348029/148‑2 (MQ=255)
                                                                                                                            tCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATcaacaa                  >  2:2110342/1‑148 (MQ=255)
                                                                                                                            tCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATcaacaa                  >  1:2430065/1‑148 (MQ=255)
                                                                                                                             cTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGGTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACaaa                 <  1:78375/148‑1 (MQ=255)
                                                                                                                              tACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAg                <  1:1979024/148‑1 (MQ=255)
                                                                                                                                 ccTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCAc             <  1:917610/148‑1 (MQ=255)
                                                                                                                                  cTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCAcc            <  2:1612234/148‑1 (MQ=255)
                                                                                                                                     aCAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCggg         <  1:95832/148‑1 (MQ=255)
                                                                                                                                     aCAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCggg         <  1:28437/148‑1 (MQ=255)
                                                                                                                                        gTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCga      >  1:2053750/1‑148 (MQ=255)
                                                                                                                                          tGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCgatg    >  1:1469573/1‑148 (MQ=255)
                                                                                                                                           gCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCgatga   <  2:209673/148‑1 (MQ=255)
                                                                                                                                            cAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCgatgat  <  1:2376157/148‑1 (MQ=255)
                                                                                                                                            cAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCgatgat  <  1:1416704/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
AATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCCGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGAT  >  CP009273/2834949‑2835236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑