Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,836,554 G→T 100% I84I (ATC→ATA)  hycH ← hydrogenase 3 maturation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,836,5540GT98.5% 661.8 / 1.0 206I84I (ATC→ATA) hycHhydrogenase 3 maturation protein
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base T (104/99);  total (104/102)
Fisher's exact test for biased strand distribution p-value = 1.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 206 total aligned reads displayed.

TCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAGGTGGTGA  >  CP009273/2836409‑2836698
                                                                                                                                                 |                                                                                                                                                
tcctcACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTAt                                                                                                                                                >  2:3124205/1‑148 (MQ=255)
 cctcACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATg                                                                                                                                               >  1:2774168/1‑148 (MQ=255)
   tcACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGAc                                                                                                                                             >  2:2350620/1‑148 (MQ=255)
    cACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACg                                                                                                                                            <  1:1212115/148‑1 (MQ=255)
     aCCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGa                                                                                                                                           <  1:823987/148‑1 (MQ=255)
     aCCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGa                                                                                                                                           >  2:347703/1‑148 (MQ=255)
      ccATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGAt                                                                                                                                          <  2:2442670/148‑1 (MQ=255)
           aaaTAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCAc                                                                                                                                     <  1:166084/148‑1 (MQ=255)
           aaaTAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCAc                                                                                                                                     <  1:1999068/148‑1 (MQ=255)
            aaTAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCAcc                                                                                                                                    >  2:1204558/1‑148 (MQ=255)
             aTAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCa                                                                                                                                   >  2:2812313/1‑148 (MQ=255)
              tAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCat                                                                                                                                  <  2:14591/148‑1 (MQ=255)
                gATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCatat                                                                                                                                <  2:3246458/148‑1 (MQ=255)
                  tGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATAttt                                                                                                                              >  1:313841/1‑148 (MQ=255)
                  tGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATAttt                                                                                                                              >  2:3473772/1‑148 (MQ=255)
                   ggCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTc                                                                                                                             >  2:1871222/1‑148 (MQ=255)
                    gCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCg                                                                                                                            >  2:1481377/1‑148 (MQ=255)
                      gTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGc                                                                                                                          <  2:624311/148‑1 (MQ=255)
                           ccTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGcc                                                                                                                     <  2:2082475/148‑1 (MQ=255)
                              gatgaATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTc                                                                                                                  >  1:2002428/1‑148 (MQ=255)
                              gatgaATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTc                                                                                                                  >  2:2977033/1‑148 (MQ=255)
                              gatgaATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTc                                                                                                                  >  2:1692909/1‑148 (MQ=255)
                              gatgaATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTc                                                                                                                  >  2:900765/1‑148 (MQ=255)
                               atgaATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTcc                                                                                                                 <  2:2783745/148‑1 (MQ=255)
                               atgaATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTcc                                                                                                                 <  2:518497/148‑1 (MQ=255)
                                  aaTATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCAtt                                                                                                              <  1:1338234/148‑1 (MQ=255)
                                    tatCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCAtttt                                                                                                            >  1:685371/1‑148 (MQ=255)
                                     atCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTg                                                                                                           >  2:1646040/1‑148 (MQ=255)
                                      tCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTgc                                                                                                          >  1:394120/1‑148 (MQ=255)
                                      tCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTgc                                                                                                          >  1:2958600/1‑148 (MQ=255)
                                       cATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTgcg                                                                                                         >  1:2845224/1‑148 (MQ=255)
                                       cATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTGTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTgcg                                                                                                         <  2:3281586/148‑1 (MQ=255)
                                         tGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTgcggc                                                                                                       >  2:3063334/1‑148 (MQ=255)
                                             gcaTACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGc                                                                                                   <  2:2453849/148‑1 (MQ=255)
                                              caTACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCt                                                                                                  >  1:2868534/1‑148 (MQ=255)
                                              caTACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCt                                                                                                  >  1:3478294/1‑148 (MQ=255)
                                              caTACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCt                                                                                                  >  1:3209444/1‑148 (MQ=255)
                                               agaCTTAACAGCAGTGGACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGGCCGGGCTTTATCGAATGGGTTGGCCATCATCTGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCtt                                                                                                 <  2:2342878/146‑1 (MQ=255)
                                               aTACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCtt                                                                                                 <  2:2102742/148‑1 (MQ=255)
                                               aTACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCtt                                                                                                 <  2:3426304/148‑1 (MQ=255)
                                               aTACTTAACAGCAGTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCtt                                                                                                 <  2:1570466/148‑1 (MQ=255)
                                                tACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTc                                                                                                <  1:957798/148‑1 (MQ=255)
                                                 aCTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCa                                                                                               >  1:3023400/1‑148 (MQ=255)
                                                   ttAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCAct                                                                                             >  2:384714/1‑148 (MQ=255)
                                                     aaCAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCActgc                                                                                           <  2:3444428/148‑1 (MQ=255)
                                                      aCAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCActgcc                                                                                          >  1:3201136/1‑148 (MQ=255)
                                                      aCAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCActgcc                                                                                          <  1:31679/148‑1 (MQ=255)
                                                      aCAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGGGTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCActgcc                                                                                          >  2:2096698/1‑148 (MQ=255)
                                                        aGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCActgcctg                                                                                        <  1:1118028/148‑1 (MQ=255)
                                                         gCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCActgcctgc                                                                                       >  1:466122/1‑148 (MQ=255)
                                                          cATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACtgcctgcc                                                                                      >  1:1023868/1‑148 (MQ=255)
                                                          cATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACtgcctgcc                                                                                      >  1:2808223/1‑148 (MQ=255)
                                                            ttGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTc                                                                                    <  1:3397585/148‑1 (MQ=255)
                                                               tACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCag                                                                                 >  1:3376277/1‑148 (MQ=255)
                                                                aCTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCaga                                                                                <  1:1500003/148‑1 (MQ=255)
                                                                 cTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCagag                                                                               >  2:1925898/1‑148 (MQ=255)
                                                                       tCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCaa                                                                         >  1:1544767/1‑148 (MQ=255)
                                                                        ccTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAAt                                                                        >  2:2678806/1‑148 (MQ=255)
                                                                                 gcGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAg                                                               >  2:1034769/1‑148 (MQ=255)
                                                                                 gcGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAg                                                               >  1:2827200/1‑148 (MQ=255)
                                                                                  cGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACAATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGa                                                              >  2:1341699/1‑148 (MQ=255)
                                                                                     aGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGatat                                                           <  1:141788/148‑1 (MQ=255)
                                                                                      gTTTGCGCTGCCCGGGCTTTATCGACTGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATAtt                                                          <  2:3211505/148‑1 (MQ=255)
                                                                                       tttGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTc                                                         <  2:3123565/148‑1 (MQ=255)
                                                                                       tttGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTc                                                         <  1:2330184/148‑1 (MQ=255)
                                                                                            gcTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATccc                                                    >  1:2895170/1‑148 (MQ=255)
                                                                                             cTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCa                                                   >  2:2356678/1‑148 (MQ=255)
                                                                                                 ccGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACggg                                               >  1:2689694/1‑148 (MQ=255)
                                                                                                 ccGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACggg                                               <  2:137968/148‑1 (MQ=255)
                                                                                                  cGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGc                                              <  1:1290581/148‑1 (MQ=255)
                                                                                                     gCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAgg                                           >  1:2329631/1‑148 (MQ=255)
                                                                                                         tATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAg                                       >  1:3110587/1‑148 (MQ=255)
                                                                                                         tATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAg                                       <  1:1167442/148‑1 (MQ=255)
                                                                                                           tCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAgcg                                     <  1:2250809/148‑1 (MQ=255)
                                                                                                              aaTGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGcgccg                                  <  1:1925898/148‑1 (MQ=255)
                                                                                                              aaTGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGcgccg                                  >  1:2368443/1‑148 (MQ=255)
                                                                                                                 gCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCtt                               <  2:965025/148‑1 (MQ=255)
                                                                                                                  cGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCTGATATTCATCCCACGGGCAGGTGAGCGCCGCTTc                              <  2:3505709/148‑1 (MQ=255)
                                                                                                                  cGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTc                              <  2:2002428/148‑1 (MQ=255)
                                                                                                                   gTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTcc                             <  2:2159826/148‑1 (MQ=255)
                                                                                                                    ttGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCa                            >  1:2435969/1‑148 (MQ=255)
                                                                                                                        ccAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCa                        >  1:595620/1‑148 (MQ=255)
                                                                                                                        ccAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCa                        <  1:647883/148‑1 (MQ=255)
                                                                                                                         cAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCaa                       <  1:2088874/148‑1 (MQ=255)
                                                                                                                         cAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCaa                       <  1:3474145/148‑1 (MQ=255)
                                                                                                                         cAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCaa                       <  1:261943/148‑1 (MQ=255)
                                                                                                                          aGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAAt                      <  2:2606941/148‑1 (MQ=255)
                                                                                                                          aGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAAt                      <  1:1668983/148‑1 (MQ=255)
                                                                                                                            cATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCg                    >  1:2630001/1‑148 (MQ=255)
                                                                                                                            cATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCg                    >  1:2235547/1‑148 (MQ=255)
                                                                                                                             aTCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGTACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGa                   <  2:553739/148‑1 (MQ=255)
                                                                                                                               cGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATa                 <  1:3003760/148‑1 (MQ=255)
                                                                                                                                    caTGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAAcccc            >  1:1248710/1‑148 (MQ=255)
                                                                                                                                     aTGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCa           <  2:1226902/148‑1 (MQ=255)
                                                                                                                                      tGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCaa          <  1:1693894/148‑1 (MQ=255)
                                                                                                                                        gTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAgg        <  1:2856245/148‑1 (MQ=255)
                                                                                                                                            aTGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAggtggt    >  2:2463744/1‑148 (MQ=255)
                                                                                                                                            aTGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAggtggt    <  2:3201136/148‑1 (MQ=255)
                                                                                                                                             tGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAGgtggtg   <  1:237568/148‑1 (MQ=255)
                                                                                                                                             tGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAGgtggtg   >  2:1699274/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAGGTGGTGA  >  CP009273/2836409‑2836698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑