Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,879,449 G→T 100% S377* (TCA→TAA)  ygcB ← R‑loop helicase‑annealase Cas3 needed for Cascade anti‑viral activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,879,4490GT100.0% 852.6 / NA 257S377* (TCA→TAA) ygcBR‑loop helicase‑annealase Cas3 needed for Cascade anti‑viral activity
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (102/155);  total (102/155)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 257 total aligned reads displayed.

ATCAATCGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTGAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGATCAATAAGTTTC  >  CP009273/2879304‑2879592
                                                                                                                                                 |                                                                                                                                               
aTCAATCGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTaa                                                                                                                                               <  2:1694253/148‑1 (MQ=255)
aTCAATCGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTaa                                                                                                                                               >  1:2766065/1‑148 (MQ=255)
aTCAATCGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTaa                                                                                                                                               >  2:2887088/1‑148 (MQ=255)
aTCAATCGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTaa                                                                                                                                               >  2:668254/1‑148 (MQ=255)
 tCAATCGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAAt                                                                                                                                              <  1:2906950/148‑1 (MQ=255)
  cAATCGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAAtt                                                                                                                                             >  2:1406325/1‑148 (MQ=255)
   aaTCGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTg                                                                                                                                            >  1:2350364/1‑148 (MQ=255)
    aTCGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGc                                                                                                                                           >  1:2508700/1‑148 (MQ=255)
     tCGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGcc                                                                                                                                          >  1:2000997/1‑148 (MQ=255)
      cGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCa                                                                                                                                         <  2:2629549/148‑1 (MQ=255)
      cGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCa                                                                                                                                         <  2:845068/148‑1 (MQ=255)
      cGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGGGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCa                                                                                                                                         <  1:1627919/148‑1 (MQ=255)
          cAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAg                                                                                                                                     <  2:1378557/148‑1 (MQ=255)
             aCGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTACTCGCGCGTGATTTTATGGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCaa                                                                                                                                  >  2:1105681/1‑148 (MQ=255)
             aCGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCaa                                                                                                                                  >  1:1433280/1‑148 (MQ=255)
             aCGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCaa                                                                                                                                  >  2:1012573/1‑148 (MQ=255)
               gCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGa                                                                                                                                <  1:3407998/148‑1 (MQ=255)
               gCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGa                                                                                                                                <  2:800523/148‑1 (MQ=255)
                 cGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGGGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAAt                                                                                                                              <  1:281376/148‑1 (MQ=255)
                   aTTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATaa                                                                                                                            >  1:553342/1‑148 (MQ=255)
                   aTTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATaa                                                                                                                            >  2:1790929/1‑148 (MQ=255)
                    tttGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAg                                                                                                                           >  2:1206186/1‑148 (MQ=255)
                      tGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGAt                                                                                                                         <  1:1231209/148‑1 (MQ=255)
                       gCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGAtt                                                                                                                        <  1:383734/148‑1 (MQ=255)
                            aGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGCTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTgggg                                                                                                                   >  1:1049109/1‑148 (MQ=255)
                             gAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGa                                                                                                                  <  2:2542349/148‑1 (MQ=255)
                             gAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGa                                                                                                                  >  1:1310699/1‑148 (MQ=255)
                               aaCACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATg                                                                                                                <  2:626659/148‑1 (MQ=255)
                                acacTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGa                                                                                                               <  2:1269029/148‑1 (MQ=255)
                                acacTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGa                                                                                                               <  2:1148896/148‑1 (MQ=255)
                                 cacTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGaa                                                                                                              >  2:3325120/1‑148 (MQ=255)
                                   cTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGaaaa                                                                                                            <  1:1513457/148‑1 (MQ=255)
                                   cTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGaaaa                                                                                                            <  1:2089624/148‑1 (MQ=255)
                                   cTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGaaaa                                                                                                            <  1:439838/148‑1 (MQ=255)
                                     ttCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATa                                                                                                          <  2:3091549/148‑1 (MQ=255)
                                      tCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATACGATTTGGGGATGaaacgac                                                                                                         >  2:2531837/1‑144 (MQ=255)
                                      tCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATaa                                                                                                         >  2:123436/1‑148 (MQ=255)
                                      tCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATaa                                                                                                         <  1:787663/148‑1 (MQ=255)
                                      tCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTGCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATaa                                                                                                         >  2:1300526/1‑148 (MQ=255)
                                       cTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAg                                                                                                        <  1:1738170/148‑1 (MQ=255)
                                          aTTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTgg                                                                                                     <  2:149991/148‑1 (MQ=255)
                                           ttGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGgc                                                                                                    <  1:2220605/148‑1 (MQ=255)
                                            tGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGgct                                                                                                   >  2:1676448/1‑148 (MQ=255)
                                                       aaCCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCtt                                                                                        <  2:464159/148‑1 (MQ=255)
                                                         ccACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTcc                                                                                      >  2:3237807/1‑148 (MQ=255)
                                                          cACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCa                                                                                     <  1:1228699/148‑1 (MQ=255)
                                                              gACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTACTCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATtct                                                                                 >  1:1748178/1‑148 (MQ=255)
                                                               acaacaCTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATtctc                                                                                >  1:3551269/1‑148 (MQ=255)
                                                                 aacaCTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGt                                                                              <  1:2192558/148‑1 (MQ=255)
                                                                   cacTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTaa                                                                            <  1:415001/148‑1 (MQ=255)
                                                                   cacTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAAGCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTaa                                                                            >  2:2138568/1‑148 (MQ=255)
                                                                        aaCCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAagcata                                                                       <  1:1344875/148‑1 (MQ=255)
                                                                         aCCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAagcatag                                                                      <  1:2629298/148‑1 (MQ=255)
                                                                          cccACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAagcatagc                                                                     <  1:345633/148‑1 (MQ=255)
                                                                            cACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAagcctagcat                                                                   >  1:978299/1‑148 (MQ=255)
                                                                            cACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAagcatagcat                                                                   <  1:1917528/148‑1 (MQ=255)
                                                                               gCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTcg                                                                <  2:2000997/148‑1 (MQ=255)
                                                                                cttcttcttGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTcgc                                                               >  1:323753/1‑148 (MQ=255)
                                                                                 ttcttcttGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTcgcg                                                              >  1:1820330/1‑148 (MQ=255)
                                                                                   cttcttGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGt                                                            >  1:3253358/1‑148 (MQ=255)
                                                                                     tcttGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAg                                                          >  1:29702/1‑148 (MQ=255)
                                                                                     tcttGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAg                                                          >  2:2928594/1‑148 (MQ=255)
                                                                                     tcttGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAg                                                          >  2:3397380/1‑148 (MQ=255)
                                                                                      cttGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGc                                                         <  1:2698896/148‑1 (MQ=255)
                                                                                       ttGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCt                                                        <  1:2245569/148‑1 (MQ=255)
                                                                                         gCCCCTGTTCAGTAATCGCGCGGGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTtg                                                      <  1:1856995/148‑1 (MQ=255)
                                                                                          ccccTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTtgt                                                     <  1:2719839/148‑1 (MQ=255)
                                                                                             cTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGtt                                                  >  1:107509/1‑148 (MQ=255)
                                                                                              tGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTg                                                 >  1:2732657/1‑148 (MQ=255)
                                                                                                ttCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTggg                                               <  2:2721627/148‑1 (MQ=255)
                                                                                                 tCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGa                                              <  2:264339/148‑1 (MQ=255)
                                                                                                  cAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAg                                             >  2:2383353/1‑148 (MQ=255)
                                                                                                    gTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAggg                                           <  1:521424/148‑1 (MQ=255)
                                                                                                        tCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCaaa                                       <  2:296227/148‑1 (MQ=255)
                                                                                                         cgcgcgTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCaaaa                                      <  2:553342/148‑1 (MQ=255)
                                                                                                           cgcgTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAAt                                    <  1:1772328/148‑1 (MQ=255)
                                                                                                             cgTGATTTTATTGATTGAAAGAGGTGGTTAAACAGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGATTCCATTCTCGTAATCATAGCATTAGCGGTAGCTAGTTTTGGTAGGGCAAAAATaa                                  >  1:2604993/1‑148 (MQ=255)
                                                                                                               tGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAaca                                <  1:1675799/148‑1 (MQ=255)
                                                                                                               tGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAaca                                <  2:3543860/148‑1 (MQ=255)
                                                                                                                gATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAacac                               <  1:1646132/148‑1 (MQ=255)
                                                                                                                gATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAacac                               >  2:2142902/1‑148 (MQ=255)
                                                                                                                gATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAacac                               >  2:2787060/1‑148 (MQ=255)
                                                                                                                 aTTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGGTGGGAGGGCAAAAATAACACt                              >  2:1345430/1‑148 (MQ=255)
                                                                                                                        tgattgaAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCa                       <  2:882366/148‑1 (MQ=255)
                                                                                                                          attgaAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAAt                     <  2:3237996/148‑1 (MQ=255)
                                                                                                                          attgaAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCAGTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAAt                     <  2:2039242/148‑1 (MQ=255)
                                                                                                                           ttgaAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAATTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGAATTCGCGGTAGCTTGTGTAGGGAGGGCAAAAATAACACTATCCGCAAtt                    >  2:2309352/1‑148 (MQ=255)
                                                                                                                            tgaAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAAttt                   <  2:3161803/148‑1 (MQ=255)
                                                                                                                             gaAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATttg                  >  1:3492522/1‑148 (MQ=255)
                                                                                                                             gaAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATttg                  <  1:638959/148‑1 (MQ=255)
                                                                                                                             gaAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATttg                  <  1:639109/148‑1 (MQ=255)
                                                                                                                              aaaGAGTTGGATAAACCGTTAATTGCCATGAGAAAGAATAAGATTTGGGGATGAAAATAAGTGGCTAGCGCTCGCTTCCATTCTCGTAACCAAAGCATTAGCGTTAGATTGTGTAGGTGGGGCAAAAATAACAAAATCCGCAATttgt                 >  1:1908244/1‑148 (MQ=255)
                                                                                                                                agagGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATttgttg               <  2:3146624/148‑1 (MQ=255)
                                                                                                                                  agGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGAt             >  1:2430591/1‑148 (MQ=255)
                                                                                                                                  agGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGAt             <  2:1910747/148‑1 (MQ=255)
                                                                                                                                  agGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGAt             <  2:1865906/148‑1 (MQ=255)
                                                                                                                                    gttgtTATACCTTTAATTGCCATGAGCAAGAATAAGATTTGTTGATGAAAATAAGTGGCTCGCGATCGCTTCCATTCTAGTAAGCATAGCATTCGAGGTAGCTTGTGTTGGGAGTGCAAAAATAACACTATCCGCAATTTGTTGTTCa           <  2:1201597/145‑1 (MQ=255)
                                                                                                                                      ggtTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGATCAAt         <  1:1168822/148‑1 (MQ=255)
                                                                                                                                       gtTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGATCAATa        <  1:1105681/148‑1 (MQ=255)
                                                                                                                                            aCCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGATCAATAAGttt   <  2:1575920/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
ATCAATCGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTGAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGATCAATAAGTTTC  >  CP009273/2879304‑2879592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑