Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,894,388 C→A 47.8% V146V (GTC→GTA)  yqcE → MFS transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,894,3880CA47.8% 139.4 / 291.6 228V146V (GTC→GTA) yqcEMFS transporter family protein
Reads supporting (aligned to +/- strand):  ref base C (48/71);  new base A (49/60);  total (97/131)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 228 total aligned reads displayed.

TGTGGGTAATGCTCTGTATTCAGATCGCCTTTGCGATAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAA  >  CP009273/2894243‑2894534
                                                                                                                                                 |                                                                                                                                                  
  tgGGTAATGCTCTGTATTCAGATCGCCTTTGCGATAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGgtg                                                                                                                                                <  2:1816207/148‑1 (MQ=255)
    ggTAATGCTCTGTATTCAGATCGCCTTTGCGATAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCCTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTa                                                                                                                                              >  1:3423782/1‑148 (MQ=255)
       aaTGCTCTGTATTCAGATCGCCTTTGCGATAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATg                                                                                                                                           <  1:2080932/148‑1 (MQ=255)
       aaTGCTCTGTATTCAGATCGCCTTTGCGATAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATg                                                                                                                                           >  2:711363/1‑148 (MQ=255)
        aTGCTCTGTATTCAGATCGCCTTTGCGATAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGt                                                                                                                                          >  2:2417434/1‑148 (MQ=255)
         tGCTCTGTATTCAGATCGCCTTTGCGATAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTc                                                                                                                                         >  2:2530981/1‑148 (MQ=255)
          gCTCTGTATTCAGATCGCCTTTGCGATAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCg                                                                                                                                        <  2:1201729/148‑1 (MQ=255)
           ctctGTATTCAGATCGCCTTTGCGATAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGc                                                                                                                                       >  1:2245011/1‑148 (MQ=255)
            tctGTATTCAGATCGCCTTTGCGATAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCt                                                                                                                                      >  2:891787/1‑148 (MQ=255)
              tGTATTCAGATCGCCTTTGCGATAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTgg                                                                                                                                    >  1:3254704/1‑148 (MQ=255)
              tGTATTCAGATCGCCTTTGCGATAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTgg                                                                                                                                    <  1:93403/148‑1 (MQ=255)
                 aTTCAGATCGCCTTTGCGATAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCgg                                                                                                                                 <  2:306864/148‑1 (MQ=255)
                  ttCAGATCGCCTTTGCGATAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGCAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGgt                                                                                                                                >  2:3127083/1‑148 (MQ=255)
                   tCAGATCGCCTTTGCGATAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGgtg                                                                                                                               >  2:605536/1‑148 (MQ=255)
                    cAGATCGCCTTTGCGATAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGgtgt                                                                                                                              <  2:1312450/148‑1 (MQ=255)
                      gATCGCCTTTGCGATAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGttt                                                                                                                            <  2:105761/148‑1 (MQ=255)
                       aTCGCCTTTGCGATAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTa                                                                                                                           <  2:433242/148‑1 (MQ=255)
                           ccTTTGCGATAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCAt                                                                                                                       <  1:1104302/148‑1 (MQ=255)
                             tttGCGATAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCAtgt                                                                                                                     <  1:3073778/148‑1 (MQ=255)
                               tGCGATAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTgg                                                                                                                   <  1:1431747/148‑1 (MQ=255)
                                 cGATAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGt                                                                                                                 <  1:3303945/148‑1 (MQ=255)
                                  gATAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTc                                                                                                                <  2:2587138/148‑1 (MQ=255)
                                  gATAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTc                                                                                                                <  2:69313/148‑1 (MQ=255)
                                    tAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCtt                                                                                                              <  2:1160155/148‑1 (MQ=255)
                                     aaCGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCttt                                                                                                             <  1:1735984/148‑1 (MQ=255)
                                     aaCGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCttt                                                                                                             <  2:2855110/148‑1 (MQ=255)
                                      acgacgATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCtttt                                                                                                            >  1:835467/1‑148 (MQ=255)
                                       cgacgaTTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTtc                                                                                                           >  2:879165/1‑148 (MQ=255)
                                       cgacgaTTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTtc                                                                                                           >  1:2139695/1‑148 (MQ=255)
                                           gaTTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGc                                                                                                       <  1:675884/148‑1 (MQ=255)
                                            aTTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCt                                                                                                      >  1:1366058/1‑148 (MQ=255)
                                             ttttAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCtt                                                                                                     <  2:2698901/148‑1 (MQ=255)
                                                    gCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCg                                                                                              >  2:2556482/1‑148 (MQ=255)
                                                    gCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCg                                                                                              <  1:3290416/148‑1 (MQ=255)
                                                        tgGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATg                                                                                          <  1:2634855/148‑1 (MQ=255)
                                                          gTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGTCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGAc                                                                                        >  1:1021637/1‑148 (MQ=255)
                                                          gTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGAc                                                                                        <  1:2967624/148‑1 (MQ=255)
                                                            cGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAg                                                                                      >  1:2099076/1‑148 (MQ=255)
                                                             ggTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGc                                                                                     >  1:2763660/1‑148 (MQ=255)
                                                                    aTTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGcc                                                                              <  2:256878/148‑1 (MQ=255)
                                                                    aTTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGcc                                                                              <  1:1071643/148‑1 (MQ=255)
                                                                     ttAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCt                                                                             <  2:2822687/148‑1 (MQ=255)
                                                                        aaGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGaa                                                                          >  2:431177/1‑148 (MQ=255)
                                                                         aGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGaaa                                                                         >  1:2567096/1‑148 (MQ=255)
                                                                           cTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAAc                                                                       >  2:2172279/1‑148 (MQ=255)
                                                                             gCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACgg                                                                     >  2:3302038/1‑148 (MQ=255)
                                                                              cATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGt                                                                    <  2:3525176/148‑1 (MQ=255)
                                                                               aTCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTTTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTc                                                                   <  2:201791/148‑1 (MQ=255)
                                                                               aTCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTc                                                                   >  1:2882843/1‑148 (MQ=255)
                                                                                    tGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTat                                                              >  2:1646997/1‑148 (MQ=255)
                                                                                     gCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTatc                                                             >  1:3543797/1‑148 (MQ=255)
                                                                                     gCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTatc                                                             <  2:1583773/148‑1 (MQ=255)
                                                                                     gCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTatc                                                             >  1:3000504/1‑148 (MQ=255)
                                                                                     gCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTatc                                                             >  1:166330/1‑148 (MQ=255)
                                                                                        tGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTatcatc                                                          >  2:486392/1‑148 (MQ=255)
                                                                                         ggCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCt                                                         <  1:950759/148‑1 (MQ=255)
                                                                                          gCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCAGTATCATCta                                                        >  2:84647/1‑148 (MQ=255)
                                                                                          gCGATCATAGCGAGAAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCTGTGTAATGTCGCTGGCGGGGTATACCAAGTGGCTCTTTTCTCGCTTTGCACAGGATGACAGCACCAGACTGAAAACGGTCATAATCATCta                                                        >  2:3215841/1‑148 (MQ=255)
                                                                                             aTCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAg                                                     <  2:2446888/148‑1 (MQ=255)
                                                                                             aTCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAg                                                     <  2:3423782/148‑1 (MQ=255)
                                                                                              tCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAgt                                                    >  1:1721257/1‑148 (MQ=255)
                                                                                               cATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAgtg                                                   <  2:2546407/148‑1 (MQ=255)
                                                                                                  aGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTgg                                                >  1:3007035/1‑148 (MQ=255)
                                                                                                  aGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTgg                                                <  1:1090625/148‑1 (MQ=255)
                                                                                                  aGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTgg                                                >  1:1231102/1‑148 (MQ=255)
                                                                                                   gCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGt                                               <  1:863762/148‑1 (MQ=255)
                                                                                                     gAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGttt                                             <  1:2082575/148‑1 (MQ=255)
                                                                                                          aGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTAGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATc                                        <  1:891780/148‑1 (MQ=255)
                                                                                                           gggAAAATTATGGGCTGGATGGAAGGTCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCt                                       <  2:1021637/148‑1 (MQ=255)
                                                                                                           gggAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCt                                       >  2:2212160/1‑148 (MQ=255)
                                                                                                           gggAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCt                                       >  2:2268623/1‑148 (MQ=255)
                                                                                                            ggAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCtt                                      >  2:1712081/1‑148 (MQ=255)
                                                                                                             gAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCttg                                     <  2:2012411/148‑1 (MQ=255)
                                                                                                             gAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCttg                                     <  1:3465983/148‑1 (MQ=255)
                                                                                                             gAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCttg                                     <  2:1775065/148‑1 (MQ=255)
                                                                                                             gAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCttg                                     <  1:1829985/148‑1 (MQ=255)
                                                                                                                   tATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTgggg                               >  1:493146/1‑148 (MQ=255)
                                                                                                                    aTGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGa                              <  1:2312327/148‑1 (MQ=255)
                                                                                                                     tGGGCTGGATGGACGGGCTGCGCGGCGTCGGTGTTATGTCGCTTGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGAt                             <  2:3229005/148‑1 (MQ=255)
                                                                                                                     tGGGCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGAt                             <  2:2622753/148‑1 (MQ=255)
                                                                                                                      gggCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGAtt                            <  1:2502456/148‑1 (MQ=255)
                                                                                                                      gggCTGGATGGAAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTTCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGAtt                            >  1:1975739/1‑148 (MQ=255)
                                                                                                                            gatggaAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGc                      <  1:2212155/148‑1 (MQ=255)
                                                                                                                             atggaAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCt                     <  2:29264/148‑1 (MQ=255)
                                                                                                                               ggaAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTgg                   <  1:2669743/148‑1 (MQ=255)
                                                                                                                                gaAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTGGt                  >  2:1473140/1‑148 (MQ=255)
                                                                                                                                gaAGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTGGt                  >  1:600736/1‑148 (MQ=255)
                                                                                                                                 aaGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTGGtt                 <  1:3188056/148‑1 (MQ=255)
                                                                                                                                 aaGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTGGtt                 <  1:2191776/148‑1 (MQ=255)
                                                                                                                                  aGGGCTGCGCGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTGGttt                <  2:3290161/148‑1 (MQ=255)
                                                                                                                                      cTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTGGttttttt            >  1:76390/1‑147 (MQ=255)
                                                                                                                                       tGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTGGTTTTTTGt           <  1:2831243/148‑1 (MQ=255)
                                                                                                                                        gcgcGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTGGTTTTTTGtt          <  2:3373103/148‑1 (MQ=255)
                                                                                                                                        gcgcGGCGTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTGGTTTTTTGtt          <  2:1366058/148‑1 (MQ=255)
                                                                                                                                           cggcgTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGc       <  1:2417434/148‑1 (MQ=255)
                                                                                                                                           cggcgTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGc       >  1:3078915/1‑148 (MQ=255)
                                                                                                                                            ggcgTAGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCg      <  1:2756307/148‑1 (MQ=255)
                                                                                                                                              cgTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGAt    <  1:567156/148‑1 (MQ=255)
                                                                                                                                              cgTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGAt    <  1:2581810/148‑1 (MQ=255)
                                                                                                                                               gTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATa   <  2:1423325/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TGTGGGTAATGCTCTGTATTCAGATCGCCTTTGCGATAACGACGATTTTAATGCTGTGGTCGGTGTCGATTAAAGCTGCATCGTTGCTTGGCGATCATAGCGAGCAAGGGAAAATTATGGGCTGGATGGAAGGGCTGCGCGGCGTCGGTGTAATGTCGCTGGCGGTGTTTACCATGTGGGTCTTTTCTCGCTTTGCACCGGATGACAGCACCAGCCTGAAAACGGTCATTATCATCTATAGTGTGGTTTACATCTTGTTGGGGATTCTGTGCTGGTTTTTTGTTAGCGATAA  >  CP009273/2894243‑2894534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑