Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,901,514 C→A 33.1% P504P (CCG→CCT)  pyrG ← CTP synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,901,5140CA33.1% 343.0 / 154.4 196P504P (CCG→CCT) pyrGCTP synthetase
Reads supporting (aligned to +/- strand):  ref base C (52/79);  new base A (35/30);  total (87/109)
Fisher's exact test for biased strand distribution p-value = 6.81e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 197 total aligned reads displayed.

TTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTG  >  CP009273/2901367‑2901660
                                                                                                                                                   |                                                                                                                                                  
ttGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTc                                                                                                                                                    >  1:2782706/1‑148 (MQ=255)
      ctctAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACt                                                                                                                                              <  1:2969430/148‑1 (MQ=255)
      ctctAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACt                                                                                                                                              <  2:65763/148‑1 (MQ=255)
       tctAACTTTTTTAGTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACTGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACtt                                                                                                                                             <  1:190535/148‑1 (MQ=255)
       tctAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACtt                                                                                                                                             >  1:2291043/1‑148 (MQ=255)
               ttttACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGAtc                                                                                                                                     <  2:121242/148‑1 (MQ=255)
               ttttACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGAtc                                                                                                                                     >  2:650235/1‑148 (MQ=255)
                  tacttactTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCg                                                                                                                                  <  2:2175758/148‑1 (MQ=255)
                  tacttactTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCg                                                                                                                                  >  2:97157/1‑148 (MQ=255)
                   acttacttCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGa                                                                                                                                 <  1:2388395/148‑1 (MQ=255)
                      tacttCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCa                                                                                                                              >  1:136474/1‑148 (MQ=255)
                        cttCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAAc                                                                                                                            <  1:2191341/148‑1 (MQ=255)
                         ttCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACt                                                                                                                           >  2:3258369/1‑148 (MQ=255)
                          tCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTg                                                                                                                          >  2:3168399/1‑148 (MQ=255)
                          tCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTg                                                                                                                          <  2:1236769/148‑1 (MQ=255)
                                gACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGGAGAAGTAAACTCCGGATGGAACTGGCACGCCACGAACCACGGGTGAGGAGGAACATCGATGATCGCGACCAACAGttcagc                                                                                                                    >  2:2142195/1‑146 (MQ=255)
                                 aCGTTTCTGGAACTCGCTGGCGGCTTTCACAAATCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAAATTCGATGATCTCTACAAACTGATCATcc                                                                                                                   >  1:597148/1‑148 (MQ=255)
                                  cGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATccc                                                                                                                  >  2:2429715/1‑148 (MQ=255)
                                    tttCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCg                                                                                                                >  1:663624/1‑148 (MQ=255)
                                    tttCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCg                                                                                                                >  2:2162518/1‑148 (MQ=255)
                                     ttCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCgg                                                                                                               >  1:1021930/1‑148 (MQ=255)
                                      tCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGa                                                                                                              <  2:379220/148‑1 (MQ=255)
                                       cTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGaa                                                                                                             <  2:1763777/148‑1 (MQ=255)
                                         ggAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACg                                                                                                           >  1:1385898/1‑148 (MQ=255)
                                           aaCTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGc                                                                                                         >  1:883435/1‑148 (MQ=255)
                                           aaCTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGc                                                                                                         >  2:165146/1‑148 (MQ=255)
                                           aaCTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAAATGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGc                                                                                                         <  1:2461954/148‑1 (MQ=255)
                                                   ggcggcTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAAcg                                                                                                 >  2:3103335/1‑148 (MQ=255)
                                                    gcggcTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAAcgc                                                                                                >  1:1035002/1‑148 (MQ=255)
                                                       gcTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCa                                                                                             <  1:1501422/148‑1 (MQ=255)
                                                       gcTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCa                                                                                             <  1:425065/148‑1 (MQ=255)
                                                       gcTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCa                                                                                             >  2:3029527/1‑148 (MQ=255)
                                                       gcTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCa                                                                                             >  2:1252618/1‑148 (MQ=255)
                                                            cacaACGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCt                                                                                        <  1:1050317/148‑1 (MQ=255)
                                                              caAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGc                                                                                      <  1:43601/148‑1 (MQ=255)
                                                                aaGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCAt                                                                                    <  1:3133302/148‑1 (MQ=255)
                                                                aaGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCAt                                                                                    <  2:2503651/148‑1 (MQ=255)
                                                                aaGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCAt                                                                                    >  2:2004265/1‑148 (MQ=255)
                                                                  gCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCt                                                                                  <  1:305056/148‑1 (MQ=255)
                                                                        aaaCAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATc                                                                            <  2:916529/148‑1 (MQ=255)
                                                                         aaCAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCt                                                                           <  1:1864753/148‑1 (MQ=255)
                                                                         aaCAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCt                                                                           >  1:2017961/1‑148 (MQ=255)
                                                                           cAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGt                                                                         <  2:2800831/148‑1 (MQ=255)
                                                                           cAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGt                                                                         <  2:1021930/148‑1 (MQ=255)
                                                                           cAGCGGGTGACCATCACGTGGAGTAAAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGt                                                                         <  1:3232162/148‑1 (MQ=255)
                                                                              cGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTc                                                                      >  2:791937/1‑148 (MQ=255)
                                                                                ggTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCaa                                                                    <  1:1939441/148‑1 (MQ=255)
                                                                                 gTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAAc                                                                   <  1:1678901/148‑1 (MQ=255)
                                                                                 gTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAAc                                                                   >  1:668100/1‑148 (MQ=255)
                                                                                    aCCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGc                                                                <  2:2321100/148‑1 (MQ=255)
                                                                                    aCCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGc                                                                >  1:3202090/1‑148 (MQ=255)
                                                                                     ccATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCa                                                               <  2:351944/148‑1 (MQ=255)
                                                                                     ccATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCa                                                               >  1:3297017/1‑148 (MQ=255)
                                                                                       aTCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCAtg                                                             >  1:1533286/1‑148 (MQ=255)
                                                                                        tCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCAtgt                                                            <  2:1196649/148‑1 (MQ=255)
                                                                                        tCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCAtgt                                                            <  2:1550942/148‑1 (MQ=255)
                                                                                        tCACGTGGAGTAGAAGTAAACTCCAGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCAtgt                                                            >  2:2668715/1‑148 (MQ=255)
                                                                                             tGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGttgttg                                                       >  2:506853/1‑148 (MQ=255)
                                                                                              ggAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGa                                                      >  2:2699986/1‑148 (MQ=255)
                                                                                               gAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGAc                                                     <  2:1502881/148‑1 (MQ=255)
                                                                                               gAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGAc                                                     <  2:1787003/148‑1 (MQ=255)
                                                                                                aGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACt                                                    <  1:3502164/148‑1 (MQ=255)
                                                                                                   aGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCg                                                 <  2:9288/148‑1 (MQ=255)
                                                                                                     aaGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAAACTGTTTCAACAGCATGTTGTTGACTTCGTa                                               <  2:406696/148‑1 (MQ=255)
                                                                                                         aaaCTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACgg                                           <  2:3036269/148‑1 (MQ=255)
                                                                                                         aaaCTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACgg                                           <  2:2490783/148‑1 (MQ=255)
                                                                                                          aaCTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGt                                          >  2:1083629/1‑148 (MQ=255)
                                                                                                          aaCTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGt                                          <  1:3298847/148‑1 (MQ=255)
                                                                                                           aCTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTg                                         <  1:2246573/148‑1 (MQ=255)
                                                                                                           aCTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTg                                         >  2:582794/1‑148 (MQ=255)
                                                                                                              ccGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACg                                      <  1:950361/148‑1 (MQ=255)
                                                                                                              ccGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACg                                      <  1:2582732/148‑1 (MQ=255)
                                                                                                              ccGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACg                                      >  1:3254654/1‑148 (MQ=255)
                                                                                                              ccGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACg                                      >  1:2841643/1‑148 (MQ=255)
                                                                                                                ggATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGAt                                    <  1:2370108/148‑1 (MQ=255)
                                                                                                                 gATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATg                                   <  2:2720253/148‑1 (MQ=255)
                                                                                                                  aTGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGa                                  >  2:2967365/1‑148 (MQ=255)
                                                                                                                    ggAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACg                                <  2:445525/148‑1 (MQ=255)
                                                                                                                    ggAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACg                                <  1:92087/148‑1 (MQ=255)
                                                                                                                      aaCTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCt                              <  1:3276090/148‑1 (MQ=255)
                                                                                                                         tGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTcaa                           >  1:239851/1‑148 (MQ=255)
                                                                                                                           gCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTcaaca                         >  1:2911356/1‑148 (MQ=255)
                                                                                                                           gCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTcaaca                         >  2:1325146/1‑148 (MQ=255)
                                                                                                                           gCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTcaaca                         <  2:1010514/148‑1 (MQ=255)
                                                                                                                           gCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTcaaca                         <  2:82470/148‑1 (MQ=255)
                                                                                                                           gCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTcaaca                         <  2:2291043/148‑1 (MQ=255)
                                                                                                                            cAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTcaacaa                        >  1:1115869/1‑148 (MQ=255)
                                                                                                                            cAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTcaacaa                        >  2:702502/1‑148 (MQ=255)
                                                                                                                               gCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTg                     <  2:2709302/148‑1 (MQ=255)
                                                                                                                               gCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTg                     >  1:2312133/1‑148 (MQ=255)
                                                                                                                               gCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTg                     >  1:2347672/1‑148 (MQ=255)
                                                                                                                               gCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTg                     <  2:1481192/148‑1 (MQ=255)
                                                                                                                                     aaCCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGgcg               <  2:2167416/148‑1 (MQ=255)
                                                                                                                                       ccACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCa             >  1:509538/1‑148 (MQ=255)
                                                                                                                                       ccACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCa             >  1:3358353/1‑148 (MQ=255)
                                                                                                                                        cACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCAt            <  1:925310/148‑1 (MQ=255)
                                                                                                                                           gggTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGt         <  2:3207282/148‑1 (MQ=255)
                                                                                                                                               gATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCACAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAg     <  1:1278536/148‑1 (MQ=255)
                                                                                                                                                aTTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGc    <  2:663624/148‑1 (MQ=255)
                                                                                                                                                  tCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTg  >  1:2762239/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTG  >  CP009273/2901367‑2901660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑